RchiOBHm_Chr4g0398261

Protein of unknown function (DUF3752)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001274
Physical Location & Seq
Reverse (-)
14751326 .. 14760920
9595 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37044

Sequence Viewer

Length: 1734 bp
ATGGGGAGTAGAAGCAAGAGGAAGAAGCATTTGCCGGAAACGAGCGACGACGATTCACCGTCGTCGTCTCCTTCAAACTCCGACGAATCCGACCGGTCCTCGAAACGGCGTCGTCGCCACCGTCACAGAAGCGACGACGGCTCCAGAAGAGAGAAAGCAAGGAGAAGGGAGAGGAGAGAGAAACGGCGAAGTAAGAGAGAGGCGAGGCGGAGCAAATCGAAGACGAAGACGAAGAAGAAGAAGAGGGACTATGACTCCGGTTCGGGGTCTTCGAGTGGTGGCGGTGATTCTGAGCCGGAGAGGAGCCGGGTTGAGCCCCGAGAGGTCTTGCGAGACTTGTTCAGTGAATTTCCTAATGTTGGCGACGATTTGAAGCAGCTTTTGCAAATGATTGATAGTGGGCAAGCAGTTGATATAAAGGGAATATCTGAAAGATCTTTGATTAAGCATCTAAAGAAGTTATTCCTTTCATTAGACCTGAAGGAAAGTGATGGAGTTTTTGTACTGCCTTCAAATGTTCGTCCCACCTTGGAGGTTGTTGGGGTCATGATTCCAACGTCTGTAGATCTTAAATCTTCATTAAATGAGATGCATTCAGAACAACGGGATGAAGAATACAAACAAGTGGTTGGTGATGATAATATGGCATCGCCATGTCCGGAGGATAATGTTGCTGGTCCTAACAGAAGGATAATTGGCCCTGCAATGCCATCGGCCGAGCTACTTGCTGCTGCAGCCAAACTGACAGAGGCACAGGCCGAGCTCAGGGAAGTGGAGTTGGAAGAGGATGCTGGAATGTTTATAGGGCCTCCACCACCTGCTGTTGTTGCTGAAGTTGAATCAGCTAATGAAGCTGAACGGTTTGAAGAGGTCACGAGGATCATAGATGCTGAGGAGAATAGTCCATATGATGTTCTAGGAGCAAACCAAAATATGGCTCCTGAGAACATAAAGAAAAGGTACTGGAAGATGTCTCTTTTGGTGCACCCTGATAAATGCTCACATCCTCAAGCTCAACAAGCATTTGTCAAGTTGAACAAAGCTTTTAAAGATTTACAAGATCCAGAAAAGCGGAAAGTGCTAGATGAGAAGATTAAACTCAAGGAGGAGCATGAGAAATTTAAGGTGGAGTTGCGAGCAATGCGGGAGGCTGCACAATGGAGAAAGTTACAAGGAATATCCATGGAGGGTGATGATGAGCTATTGGCAGACATGGATGTTAAAGTGGCACCACAAAGAGATGAGTGGATGACAACGCTTCCTCCTGAGAGAAGACCTGGTGGTATGCCTACACAGTCAACAAAGTTTAGCAGCACCGTTAAAGAAGGACGTGGTGATACTAGTGCATGGACTGATACCCCTTCTGACAGAGCCCAGAAAGCTAAGATGGATTACTTAGAAGCATATAATGAGGCTGCATCACTTGCTTCAAATGAGGAGAAGAAAATGAATAGTTCAGATGCAGATTTGGTGGACAGATACAACAAAGAAAAACGATCAAAATCATTGGTACAAAAACACCAAGAGACTGCAAGCCGCCCGAAGAGAAAATCCAAGCAACCGTTGGAGAAGGAGAAGAAGGAAGAATGGGTGGGAAAACATCCTTGGAAGCCTTGGGACCGTGAAAAGGATTTGACAGGCGGTAGGCAAAGTGTAAAATTGGATTCAGAAAACATGGCTCAAGGTTTAACGTCTCGGTTTTCTGCGGGCTCCTTTCAGAGAAACTTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001664 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0050780
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3752 DnaJ

Protein Analysis

577

Amino Acids

65.58

Weight (kDa)

9.0

Isoelectric Point (pI)

65.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 302 - 362 4.2e-17 DnaJ domain
DUF3752 PF12572 421 - 568 4.6e-28 Protein of unknown function (DUF3752)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 826
Acc36I ACCTGC 1 cut(s) 826
AccB1I GGYRCC 1 cut(s) 1228
AccB7I CCANNNNNTGG 1 cut(s) 934
AccIII TCCGGA 1 cut(s) 658
AciI CCGC 7 cut(s) 208, 282, 1072, 1144, 1537, 1641, 1706
AclWI GGATC 2 cut(s) 887, 1055
AcoI YGGCCR 1 cut(s) 714
AcsI RAATTY 2 cut(s) 347, 1118
AcuI CTGAAG 2 cut(s) 500, 852
AcyI GRCGYC 1 cut(s) 109
AfaI GTAC 3 cut(s) 504, 962, 1512
AfiI CCNNNNNNNGG 6 cut(s) 105, 264, 359, 765, 934, 1627
AgeI ACCGGT 1 cut(s) 93
AgsI TTSAA 7 cut(s) 75, 373, 513, 839, 866, 1036, 1431
AhlI ACTAGT 1 cut(s) 1340
AjiI CACGTC 1 cut(s) 1331
AjnI CCWGG 1 cut(s) 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 1588, 1620
AluBI AGCT 9 cut(s) 379, 721, 763, 845, 854, 1013, 1043, 1201, 1382
AluI AGCT 9 cut(s) 379, 721, 763, 845, 854, 1013, 1043, 1201, 1382
Alw21I GWGCWC 2 cut(s) 765, 987
Alw26I GTCTC 5 cut(s) 72, 327, 978, 1520, 1698
Alw44I GTGCAC 1 cut(s) 983
AlwI GGATC 2 cut(s) 887, 1055
Ama87I CYCGRG 1 cut(s) 318
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 4 cut(s) 697, 714, 756, 806
ApaLI GTGCAC 1 cut(s) 983
ApeKI GCWGC 7 cut(s) 376, 728, 731, 734, 1151, 1311, 1415
ApoI RAATTY 2 cut(s) 347, 1118
AsiGI ACCGGT 1 cut(s) 93
Asp700I GAANNNNTTC 1 cut(s) 461
AspS9I GGNCC 5 cut(s) 96, 677, 698, 806, 1618
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 5 cut(s) 48, 296, 644, 1202, 1346
AvaI CYCGRG 1 cut(s) 318
AvaII GGWCC 3 cut(s) 96, 677, 1618
BaeGI GKGCMC 1 cut(s) 987
BanI GGYRCC 1 cut(s) 1228
BanII GRGCYC 4 cut(s) 318, 765, 1375, 1712
BauI CACGAG 1 cut(s) 874
BbsI GAAGAC 4 cut(s) 227, 233, 261, 1279
Bbv12I GWGCWC 2 cut(s) 765, 987
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 7 cut(s) 388, 715, 718, 746, 1138, 1323, 1402
BccI CCATC 3 cut(s) 485, 718, 1381
BceAI ACGGC 3 cut(s) 122, 154, 200
BcgI CGANNNNNNTGC 4 cut(s) 693, 707, 727, 741
BciT130I CCWGG 1 cut(s) 1278
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 5 cut(s) 72, 327, 978, 1520, 1698
BcuI ACTAGT 1 cut(s) 1340
BfaI CTAG 3 cut(s) 917, 1082, 1341
BfmI CTRYAG 2 cut(s) 561, 732
BfuAI ACCTGC 1 cut(s) 826
BglII AGATCT 2 cut(s) 434, 565
BisI GCNGC 8 cut(s) 377, 729, 732, 735, 1152, 1312, 1416, 1537
BlsI GCNGC 8 cut(s) 378, 730, 733, 736, 1153, 1313, 1417, 1538
Bme1390I CCNGG 2 cut(s) 308, 1278
Bme18I GGWCC 3 cut(s) 96, 677, 1618
BmeT110I CYCGRG 1 cut(s) 318
BmgBI CACGTC 1 cut(s) 1331
BmgT120I GGNCC 5 cut(s) 96, 677, 698, 806, 1618
BmiI GGNNCC 6 cut(s) 142, 305, 939, 1230, 1619, 1711
BmrFI CCNGG 2 cut(s) 308, 1278
BmsI GCATC 7 cut(s) 457, 579, 656, 778, 877, 1427, 1450
BpiI GAAGAC 4 cut(s) 227, 233, 261, 1279
BpmI CTGGAG 1 cut(s) 127
Bpu10I CCTNAGC 2 cut(s) 764, 891
BpuEI CTTGAG 3 cut(s) 993, 1085, 1665
BpuMI CCSGG 1 cut(s) 308
BsaBI GATNNNNATC 1 cut(s) 1501
BsaHI GRCGYC 1 cut(s) 109
BsaJI CCNNGG 4 cut(s) 528, 1182, 1604, 1613
BsaWI WCCGGW 3 cut(s) 93, 257, 658
BsaXI ACNNNNNCTCC 8 cut(s) 125, 155, 239, 269, 486, 516, 1246, 1276
Bsc4I CCNNNNNNNGG 6 cut(s) 105, 264, 359, 765, 934, 1627
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 968
Bse3DI GCAATG 2 cut(s) 711, 1146
Bse8I GATNNNNATC 1 cut(s) 1501
BseAI TCCGGA 1 cut(s) 658
BseBI CCWGG 1 cut(s) 1278
BseDI CCNNGG 4 cut(s) 528, 1182, 1604, 1613
BseGI GGATG 6 cut(s) 613, 793, 1003, 1222, 1254, 1600
BseJI GATNNNNATC 1 cut(s) 1501
BseLI CCNNNNNNNGG 6 cut(s) 105, 264, 359, 765, 934, 1627
BseMI GCAATG 2 cut(s) 711, 1146
BseMII CTCAG 5 cut(s) 282, 778, 882, 933, 1257
BseNI ACTGG 1 cut(s) 968
BseRI GAGGAG 5 cut(s) 187, 316, 908, 1121, 1451
BseSI GKGCMC 1 cut(s) 987
BseX3I CGGCCG 1 cut(s) 714
BseXI GCAGC 7 cut(s) 388, 715, 718, 746, 1138, 1323, 1402
BsgI GTGCAG 1 cut(s) 1137
Bsh1285I CGRYCG 2 cut(s) 94, 717
BshFI GGCC 4 cut(s) 699, 716, 758, 808
BshNI GGYRCC 1 cut(s) 1228
BshTI ACCGGT 1 cut(s) 93
BsiEI CGRYCG 2 cut(s) 94, 717
BsiHKAI GWGCWC 2 cut(s) 765, 987
BsiHKCI CYCGRG 1 cut(s) 318
BsiSI CCGG 6 cut(s) 35, 94, 258, 296, 307, 659
BslFI GGGAC 3 cut(s) 260, 507, 1631
BslI CCNNNNNNNGG 6 cut(s) 105, 264, 359, 765, 934, 1627
BsmAI GTCTC 5 cut(s) 72, 327, 978, 1520, 1698
BsmBI CGTCTC 2 cut(s) 72, 1698
BsmFI GGGAC 3 cut(s) 260, 507, 1631
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 4 cut(s) 699, 716, 758, 808
BsoBI CYCGRG 1 cut(s) 318
Bsp1286I GDGCHC 5 cut(s) 318, 765, 987, 1375, 1712
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 5 cut(s) 434, 565, 879, 1060, 1496
Bsp19I CCATGG 1 cut(s) 1182
BspACI CCGC 7 cut(s) 208, 282, 1072, 1144, 1537, 1641, 1706
BspANI GGCC 4 cut(s) 699, 716, 758, 808
BspCNI CTCAG 5 cut(s) 283, 777, 883, 934, 1258
BspEI TCCGGA 1 cut(s) 658
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 6 cut(s) 142, 305, 939, 1230, 1619, 1711
BspMAI CTGCAG 1 cut(s) 736
BspMI ACCTGC 1 cut(s) 826
BspPI GGATC 2 cut(s) 887, 1055
BspT107I GGYRCC 1 cut(s) 1228
BsrDI GCAATG 2 cut(s) 711, 1146
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 1 cut(s) 968
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 4 cut(s) 528, 1182, 1604, 1613
BssMI GATC 5 cut(s) 434, 565, 879, 1060, 1496
BssNI GRCGYC 1 cut(s) 109
BssSI CACGAG 1 cut(s) 874
BssT1I CCWWGG 4 cut(s) 528, 1182, 1604, 1613
Bst2BI CACGAG 1 cut(s) 874
Bst2UI CCWGG 1 cut(s) 1278
Bst4CI ACNGT 7 cut(s) 60, 122, 861, 1296, 1318, 1563, 1622
Bst6I CTCTTC 5 cut(s) 142, 236, 777, 861, 1538
BstACI GRCGYC 1 cut(s) 109
BstAPI GCANNNNNTGC 2 cut(s) 382, 1424
BstC8I GCNNGC 4 cut(s) 405, 1137, 1534, 1708
BstDEI CTNAG 7 cut(s) 291, 764, 891, 942, 1266, 1383, 1396
BstDSI CCRYGG 1 cut(s) 1182
BstF5I GGATG 6 cut(s) 613, 793, 1003, 1222, 1254, 1600
BstKTI GATC 5 cut(s) 437, 568, 882, 1063, 1499
BstMAI GTCTC 5 cut(s) 72, 327, 978, 1520, 1698
BstMBI GATC 5 cut(s) 434, 565, 879, 1060, 1496
BstMCI CGRYCG 2 cut(s) 94, 717
BstNI CCWGG 1 cut(s) 1278
BstSCI CCNGG 2 cut(s) 306, 1276
BstSFI CTRYAG 2 cut(s) 561, 732
BstSLI GKGCMC 1 cut(s) 987
BstV1I GCAGC 7 cut(s) 388, 715, 718, 746, 1138, 1323, 1402
BstV2I GAAGAC 4 cut(s) 227, 233, 261, 1279
BstX2I RGATCY 3 cut(s) 434, 565, 1060
BstYI RGATCY 3 cut(s) 434, 565, 1060
BstZI CGGCCG 1 cut(s) 714
BsuRI GGCC 4 cut(s) 699, 716, 758, 808
BtgI CCRYGG 1 cut(s) 1182
BtgZI GCGATG 1 cut(s) 633
BtrI CACGTC 1 cut(s) 1331
BtsCI GGATG 6 cut(s) 613, 793, 1003, 1222, 1254, 1600
BtsIMutI CAGTG 1 cut(s) 349
BveI ACCTGC 1 cut(s) 826
Cac8I GCNNGC 4 cut(s) 405, 1137, 1534, 1708
CciI TCATGA 1 cut(s) 546
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 5 cut(s) 96, 677, 698, 806, 1618
CseI GACGC 1 cut(s) 98
CsiI ACCWGGT 1 cut(s) 1276
Csp6I GTAC 3 cut(s) 503, 961, 1511
CspAI ACCGGT 1 cut(s) 93
CviAII CATG 7 cut(s) 547, 654, 1112, 1183, 1213, 1347, 1675
CviQI GTAC 3 cut(s) 503, 961, 1511
DdeI CTNAG 7 cut(s) 291, 764, 891, 942, 1266, 1383, 1396
DpnI GATC 5 cut(s) 436, 567, 881, 1062, 1498
DpnII GATC 5 cut(s) 434, 565, 879, 1060, 1496
DraI TTTAAA 1 cut(s) 1048
EaeI YGGCCR 1 cut(s) 714
EagI CGGCCG 1 cut(s) 714
Eam1104I CTCTTC 5 cut(s) 142, 236, 777, 861, 1538
EarI CTCTTC 5 cut(s) 142, 236, 777, 861, 1538
EciI GGCGGA 1 cut(s) 223
Ecl136II GAGCTC 1 cut(s) 763
EclXI CGGCCG 1 cut(s) 714
Eco130I CCWWGG 4 cut(s) 528, 1182, 1604, 1613
Eco24I GRGCYC 4 cut(s) 318, 765, 1375, 1712
Eco47I GGWCC 3 cut(s) 96, 677, 1618
Eco52I CGGCCG 1 cut(s) 714
Eco53kI GAGCTC 1 cut(s) 763
Eco57I CTGAAG 2 cut(s) 500, 852
Eco88I CYCGRG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 763
EcoO109I RGGNCCY 1 cut(s) 806
EcoRII CCWGG 1 cut(s) 1276
EcoT14I CCWWGG 4 cut(s) 528, 1182, 1604, 1613
EcoT22I ATGCAT 1 cut(s) 594
EcoT38I GRGCYC 4 cut(s) 318, 765, 1375, 1712
ErhI CCWWGG 4 cut(s) 528, 1182, 1604, 1613
Esp3I CGTCTC 2 cut(s) 72, 1698
FaeI CATG 7 cut(s) 550, 657, 1115, 1186, 1216, 1350, 1678
FaqI GGGAC 3 cut(s) 260, 507, 1631
FatI CATG 7 cut(s) 546, 653, 1111, 1182, 1212, 1346, 1674
FauI CCCGC 2 cut(s) 1137, 1699
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 8 cut(s) 377, 729, 732, 735, 1152, 1312, 1416, 1537
FokI GGATG 6 cut(s) 620, 800, 990, 1229, 1261, 1587
FriOI GRGCYC 4 cut(s) 318, 765, 1375, 1712
Fsp4HI GCNGC 8 cut(s) 377, 729, 732, 735, 1152, 1312, 1416, 1537
FspBI CTAG 3 cut(s) 917, 1082, 1341
GluI GCNGC 8 cut(s) 377, 729, 732, 735, 1152, 1312, 1416, 1537
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 4 cut(s) 699, 716, 758, 808
HapII CCGG 6 cut(s) 35, 94, 258, 296, 307, 659
HgaI GACGC 1 cut(s) 98
Hin1I GRCGYC 1 cut(s) 109
Hin1II CATG 7 cut(s) 550, 657, 1115, 1186, 1216, 1350, 1678
HincII GTYRAC 1 cut(s) 1299
HindII GTYRAC 1 cut(s) 1299
HindIII AAGCTT 1 cut(s) 1041
HinfI GANTC 7 cut(s) 53, 86, 254, 287, 550, 839, 1664
HpaII CCGG 6 cut(s) 35, 94, 258, 296, 307, 659
HphI GGTGA 5 cut(s) 48, 296, 644, 1202, 1346
Hpy166II GTNNAC 3 cut(s) 985, 1299, 1474
Hpy188I TCNGA 9 cut(s) 82, 91, 292, 430, 598, 1366, 1459, 1669, 1719
Hpy188III TCNNGA 7 cut(s) 144, 547, 659, 874, 941, 1064, 1265
Hpy8I GTNNAC 3 cut(s) 985, 1299, 1474
HpyAV CCTTC 9 cut(s) 81, 159, 475, 519, 681, 1319, 1371, 1564, 1573
HpyCH4III ACNGT 7 cut(s) 60, 122, 861, 1296, 1318, 1563, 1622
HpyCH4IV ACGT 3 cut(s) 557, 1330, 1691
HpyF3I CTNAG 7 cut(s) 291, 764, 891, 942, 1266, 1383, 1396
HpySE526I ACGT 3 cut(s) 557, 1330, 1691
Hsp92I GRCGYC 1 cut(s) 109
Hsp92II CATG 7 cut(s) 550, 657, 1115, 1186, 1216, 1350, 1678
Kpn2I TCCGGA 1 cut(s) 658
Kzo9I GATC 5 cut(s) 434, 565, 879, 1060, 1496
LmnI GCTCC 7 cut(s) 146, 210, 303, 920, 943, 1108, 1715
Lsp1109I GCAGC 7 cut(s) 388, 715, 718, 746, 1138, 1323, 1402
LweI GCATC 7 cut(s) 457, 579, 656, 778, 877, 1427, 1450
MabI ACCWGGT 1 cut(s) 1276
MaeI CTAG 3 cut(s) 917, 1082, 1341
MaeII ACGT 3 cut(s) 557, 1330, 1691
MaeIII GTNAC 3 cut(s) 122, 871, 1167
MalI GATC 5 cut(s) 436, 567, 881, 1062, 1498
MboI GATC 5 cut(s) 434, 565, 879, 1060, 1496
MflI RGATCY 3 cut(s) 434, 565, 1060
MhlI GDGCHC 5 cut(s) 318, 765, 987, 1375, 1712
MluCI AATT 4 cut(s) 347, 693, 1118, 1658
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 5 cut(s) 105, 114, 578, 759, 1545
Mph1103I ATGCAT 1 cut(s) 594
MroI TCCGGA 1 cut(s) 658
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 9 cut(s) 444, 570, 581, 1047, 1095, 1122, 1221, 1320, 1688
MslI CAYNNNNRTG 1 cut(s) 652
MspI CCGG 6 cut(s) 35, 94, 258, 296, 307, 659
MspR9I CCNGG 2 cut(s) 308, 1278
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 1278
NciI CCSGG 1 cut(s) 308
NcoI CCATGG 1 cut(s) 1182
NdeI CATATG 1 cut(s) 907
NdeII GATC 5 cut(s) 434, 565, 879, 1060, 1496
NlaIII CATG 7 cut(s) 550, 657, 1115, 1186, 1216, 1350, 1678
NlaIV GGNNCC 6 cut(s) 142, 305, 939, 1230, 1619, 1711
NmeAIII GCCGAG 2 cut(s) 742, 784
NmuCI GTSAC 2 cut(s) 122, 871
NsiI ATGCAT 1 cut(s) 594
PagI TCATGA 1 cut(s) 546
PaqCI CACCTGC 1 cut(s) 826
PcsI WCGNNNNNNNCGW 2 cut(s) 118, 269
PctI GAATGC 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 6 cut(s) 53, 86, 287, 550, 839, 1664
PflMI CCANNNNNTGG 1 cut(s) 934
PinAI ACCGGT 1 cut(s) 93
PkrI GCNGC 8 cut(s) 378, 730, 733, 736, 1153, 1313, 1417, 1538
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 1 cut(s) 1276
PspGI CCWGG 1 cut(s) 1276
PspN4I GGNNCC 6 cut(s) 142, 305, 939, 1230, 1619, 1711
PspPI GGNCC 5 cut(s) 96, 677, 698, 806, 1618
PstI CTGCAG 1 cut(s) 736
PsuI RGATCY 3 cut(s) 434, 565, 1060
RsaI GTAC 3 cut(s) 504, 962, 1512
RsaNI GTAC 3 cut(s) 503, 961, 1511
RseI CAYNNNNRTG 1 cut(s) 652
SacI GAGCTC 1 cut(s) 765
SaqAI TTAA 9 cut(s) 444, 570, 581, 1047, 1095, 1122, 1221, 1320, 1688
SatI GCNGC 8 cut(s) 377, 729, 732, 735, 1152, 1312, 1416, 1537
Sau3AI GATC 5 cut(s) 434, 565, 879, 1060, 1496
Sau96I GGNCC 5 cut(s) 96, 677, 698, 806, 1618
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 308, 1278
SduI GDGCHC 5 cut(s) 318, 765, 987, 1375, 1712
SexAI ACCWGGT 1 cut(s) 1276
SfaNI GCATC 7 cut(s) 457, 579, 656, 778, 877, 1427, 1450
SfcI CTRYAG 2 cut(s) 561, 732
SinI GGWCC 3 cut(s) 96, 677, 1618
SmiMI CAYNNNNRTG 1 cut(s) 652
SmlI CTYRAG 3 cut(s) 1008, 1100, 1680
SmoI CTYRAG 3 cut(s) 1008, 1100, 1680
SpeI ACTAGT 1 cut(s) 1340
Sse9I AATT 4 cut(s) 347, 693, 1118, 1658
SsiI CCGC 7 cut(s) 208, 282, 1072, 1144, 1537, 1641, 1706
SspMI CTAG 3 cut(s) 917, 1082, 1341
SstI GAGCTC 1 cut(s) 765
StyD4I CCNGG 2 cut(s) 306, 1276
StyI CCWWGG 4 cut(s) 528, 1182, 1604, 1613
TaaI ACNGT 7 cut(s) 60, 122, 861, 1296, 1318, 1563, 1622
TaiI ACGT 3 cut(s) 560, 1333, 1694
TaqI TCGA 3 cut(s) 101, 218, 272
TasI AATT 4 cut(s) 347, 693, 1118, 1658
TatI WGTACW 1 cut(s) 502
TauI GCSGC 1 cut(s) 1539
TfiI GAWTC 6 cut(s) 53, 86, 287, 550, 839, 1664
Tru1I TTAA 9 cut(s) 444, 570, 581, 1047, 1095, 1122, 1221, 1320, 1688
Tru9I TTAA 9 cut(s) 444, 570, 581, 1047, 1095, 1122, 1221, 1320, 1688
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 2 cut(s) 122, 871
TseI GCWGC 7 cut(s) 376, 728, 731, 734, 1151, 1311, 1415
Tsp45I GTSAC 2 cut(s) 122, 871
TspDTI ATGAA 5 cut(s) 459, 567, 624, 864, 1463
TspRI CASTG 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 934
VneI GTGCAC 1 cut(s) 983
VpaK11BI GGWCC 3 cut(s) 96, 677, 1618
XapI RAATTY 2 cut(s) 347, 1118
XcmI CCANNNNNNNNNTGG 1 cut(s) 1561
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 3 cut(s) 917, 1082, 1341
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.