Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001274

Physical Location & Seq

Genomic Coordinates Forward (+)

506117 .. 514115

Gene Structure 7999 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1003500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGGGAAGCAAAAGCAAAAGCAAGAGAAGGAAGCAATTGGAGGATTCAACCGACGACGATTCATATTCATCGCCTTCCTCAAGCTCCGAGGAATCCGAGCGCTCTTCGAAAAGGCACCGTCGCAGAAAAGACGACGGTGGTTCGAGAAGAGAGAAGGAACGGAGAGAGAAACGGAGAAGCAAGCGAGAGACGAAGAGAAGGAAATCAAAGAAGAAGAAGAGGGCTTATGGGGTTGATGACGGCGATGAGTCCGGTTCTGGGAGGAGTGCTGACGATTCAGAGCCAGGCCGGAGTCGGCTCGATCCGCAACCCGTTTTACGAGAATTGTTCATCGAATTTCCCAATGTTGGGAATGATTTGAAACAGCTTTTAGAAATGATTGATTGTGGGCAAGCAGTTGACATCAAGGGAATTTCTGAAAGATCTTTGATTAAGCATCTGAAGAAGTTGTTCCATTCATTAGACCTCAAAGAAAATAATGGGGTCTTTTTGTTGCCTTCAAATGTTCGTCCCACTTTGGAGGTTGTTGGGCTTATGATTCAAAAGTCTTTAGAACCTAAAACTGGACAGAATGAGGTGCATCCAGAACAACCAGATGAGGAATACAATCAAGTGGTTGATGAAAATAAAACGACGGTACGATGCATGGATGATGTTGATGCTGGTCCTAAAAGAAGGACAATTGGCCCTGCAATGCCATCAGCTGAGTTACTTGCAGCAGCAGCCAAATTGACGGAGGCACAGGCTGAGCTCAGAGAAGCTGAGTTGGAAGAAGATTCTGAATATTTTATAGGACCTCCACCACCAGCCGTGGTTGCTGAAACTGAATCAGCTAATGAAGCTGAGCGGTTTGAAGAGGTCACAAGGATCATGGATGCTGAGGATGATAGTCTATATGATATTTTAGGAGCGAACAACAATATGTCTGCTGAGAATATTAAGAAAAGGTACTGGAAGATGTCTCTTTTGGTGCATCCTGATAAATGCTCACATCCTCAAGCTCACCAAGCATTTGTTAAGTTGAATAAAGCTTTTAAGGAACTACAAGATCCAGATAAGCGTAAAGTGCTGGATGACAAGATTAAACTCAAGGAGGAACATGAAAAATTTAAGGTTGAGTTGAAAGCTATGCGTGAGGCAGCACAATGGAGAAGGTTACAAGGAATATCCATGGAGGGTGATGATGAACTCCTAGCAGACATGGATGTTAAAGTAGCACCGAAAAGAGATGAGTGGATGACAACGCTGCCACCTGAAAAAAAACATGGTGTTGTGAGCATGCAATCAACAAGATTCAACAAGAGCACGAAAGAAGGACGTGGTGATACTAGTGCATGGACTGATACCCCTTCAGACAGAGCCCAAAAAGCTAAGATGGGTTACTTAGAAGCGTATAATCAGGCTGCAGCCCTTGCTTCGAATGAGGAGGAAAATAAAAGGCAGAGTTCAGATGCCGATTTGGTGGACAAATACAACAAAGCAAAACGATCAAAATCATTGGTACAAAAGCACAAAGAGGAGACTGCAAGCCGTTCGAAAAGAAAATCCAAGCAACAGTCACAACAGGCAGAGGAAGAATGGGTGGGGAAACATCCGTGGAAACCTTGGGATCGCGAGAAGGATTTGGCAGGTGGGAGGCAAAATGTAAAGTTGGATTCAGAAAACATGACTCAAGTTTTATCGTCTCGGTTTTCATCTGGCTCGTACGAGAGGAACTTCCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001664 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0050780

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3752 DnaJ

Protein Analysis

575

Amino Acids

65.49

Weight (kDa)

8.34

Isoelectric Point (pI)

61.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	298 - 358	1.4e-17	DnaJ domain
DUF3752	PF12572	417 - 565	4.2e-27	Protein of unknown function (DUF3752)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001274)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65280 AT1G65280
fragaria_vesca	FvH4_3g44892 FvH4_3g44892 FvH4_3g44892 FvH4_3g44931 FvH4_3g44932 FvH4_3g44960 FvH4_3g44966 FvH4_4g06470
malus_domestica	MD04G1003500.v1.1
prunus_persica	Prupe.8G058000_v2.0.a1 Prupe.8G058000_v2.0.a1 Prupe.8G058000_v2.0.a1 Prupe.8G058000_v2.0.a1
pyrus_communis	pycom04g00360
rosa_chinensis	RchiOBHm_Chr4g0398261 RchiOBHm_Chr5g0081671 RchiOBHm_Chr5g0082081 RchiOBHm_Chr5g0082701
rosa_laevigata	RLG00000036957 RLG00000036962 RLG00000036979
rosa_multiflora	Rmu_co8467303.1_g000001 Rmu_sc0000303.1_g000009 Rmu_sc0001687.1_g000002 Rmu_sc0002176.1_g000036 Rmu_sc0002723.1_g000006 Rmu_sc0002723.1_g000051 Rmu_sc0002723.1_g000052 Rmu_sc0004094.1_g000006 Rmu_sc0004835.1_g000037 Rmu_sc0012399.1_g000001
rosa_roxburghii	Rroxscaffold_5G00343240
rosa_rugosa	Rorug04G0007200 Rorug04G0007300 Rorug05G0477600
rosa_samantha	Rh4AG084700 Rh4BG082100 Rh4CG092100 Rh4DG077200 Rh5AG529100 Rh5BG554300 Rh5BG554800 Rh5BG555700 Rh5CG577200 Rh5CG577700 Rh5CG578400 Rh5DG563900 Rh5DG564100 Rh5DG565000 Rh5DG565100
rosa_wichuraiana	Rw0G022970 Rw4G007040 Rw5G049400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1619
Acc36I	ACCTGC	1 cut(s)	1619
AccB1I	GGYRCC	1 cut(s)	114
AccBSI	CCGCTC	1 cut(s)	847
AccII	CGCG	1 cut(s)	1614
AciI	CCGC	2 cut(s)	305, 847
AclWI	GGATC	4 cut(s)	296, 875, 1043, 1617
AcsI	RAATTY	3 cut(s)	335, 411, 1106
AcuI	CTGAAG	2 cut(s)	461, 1335
AfaI	GTAC	4 cut(s)	639, 950, 1503, 1706
AfeI	AGCGCT	1 cut(s)	101
AfiI	CCNNNNNNNGG	4 cut(s)	258, 347, 348, 563
AgsI	TTSAA	8 cut(s)	48, 361, 501, 542, 854, 1024, 1123, 1297
AhlI	ACTAGT	1 cut(s)	1328
AjiI	CACGTC	1 cut(s)	1319
AjnI	CCWGG	1 cut(s)	283
Alw21I	GWGCWC	2 cut(s)	753, 1307
Alw26I	GTCTC	4 cut(s)	182, 966, 1514, 1689
AlwI	GGATC	4 cut(s)	296, 875, 1043, 1617
Aor51HI	AGCGCT	1 cut(s)	101
AoxI	GGCC	2 cut(s)	286, 685
ApeKI	GCWGC	7 cut(s)	716, 719, 722, 1139, 1246, 1403, 1406
ApoI	RAATTY	3 cut(s)	335, 411, 1106
Asp700I	GAANNNNTTC	1 cut(s)	449
AspLEI	GCGC	1 cut(s)	102
AspS9I	GGNCC	3 cut(s)	665, 686, 794
AsuHPI	GGTGA	3 cut(s)	995, 1190, 1334
AsuII	TTCGAA	3 cut(s)	107, 1418, 1535
AvaII	GGWCC	2 cut(s)	665, 794
BanI	GGYRCC	1 cut(s)	114
BanII	GRGCYC	2 cut(s)	753, 1363
Bbv12I	GWGCWC	2 cut(s)	753, 1307
BbvCI	CCTCAGC	1 cut(s)	879
BbvI	GCAGC	7 cut(s)	728, 731, 734, 1151, 1233, 1390, 1418
BccI	CCATC	2 cut(s)	706, 1369
BceAI	ACGGC	3 cut(s)	256, 794, 1515
BcgI	CGANNNNNNTGC	2 cut(s)	1515, 1549
BciT130I	CCWGG	1 cut(s)	285
BcoDI	GTCTC	4 cut(s)	182, 966, 1514, 1689
BcuI	ACTAGT	1 cut(s)	1328
BfaI	CTAG	2 cut(s)	1193, 1329
BfmI	CTRYAG	1 cut(s)	1404
BfoI	RGCGCY	1 cut(s)	103
BfuAI	ACCTGC	1 cut(s)	1619
BglII	AGATCT	1 cut(s)	422
BisI	GCNGC	7 cut(s)	717, 720, 723, 1140, 1247, 1404, 1407
BlpI	GCTNAGC	2 cut(s)	747, 843
BlsI	GCNGC	7 cut(s)	718, 721, 724, 1141, 1248, 1405, 1408
Bme1390I	CCNGG	1 cut(s)	285
Bme18I	GGWCC	2 cut(s)	665, 794
BmgBI	CACGTC	1 cut(s)	1319
BmgT120I	GGNCC	3 cut(s)	665, 686, 794
BmiI	GGNNCC	1 cut(s)	116
BmrFI	CCNGG	1 cut(s)	285
BmsI	GCATC	7 cut(s)	445, 589, 632, 649, 865, 982, 1441
Bpu10I	CCTNAGC	1 cut(s)	879
Bpu1102I	GCTNAGC	2 cut(s)	747, 843
Bpu14I	TTCGAA	3 cut(s)	107, 1418, 1535
BpuEI	CTTGAG	4 cut(s)	64, 981, 1073, 1656
BsaBI	GATNNNNATC	1 cut(s)	1492
BsaJI	CCNNGG	5 cut(s)	87, 810, 1170, 1595, 1604
BsaWI	WCCGGW	1 cut(s)	251
Bsc4I	CCNNNNNNNGG	4 cut(s)	258, 347, 348, 563
Bse1I	ACTGG	2 cut(s)	568, 956
Bse3DI	GCAATG	1 cut(s)	699
Bse8I	GATNNNNATC	1 cut(s)	1492
BseBI	CCWGG	1 cut(s)	285
BseDI	CCNNGG	5 cut(s)	87, 810, 1170, 1595, 1604
BseJI	GATNNNNATC	1 cut(s)	1492
BseLI	CCNNNNNNNGG	4 cut(s)	258, 347, 348, 563
BseMI	GCAATG	1 cut(s)	699
BseMII	CTCAG	7 cut(s)	696, 738, 753, 766, 834, 870, 921
BseNI	ACTGG	2 cut(s)	568, 956
BseRI	GAGGAG	3 cut(s)	277, 1439, 1532
BseXI	GCAGC	7 cut(s)	728, 731, 734, 1151, 1233, 1390, 1418
Bsh1236I	CGCG	1 cut(s)	1614
BshFI	GGCC	2 cut(s)	288, 687
BshNI	GGYRCC	1 cut(s)	114
BsiHKAI	GWGCWC	2 cut(s)	753, 1307
BsiSI	CCGG	2 cut(s)	252, 289
BsiWI	CGTACG	1 cut(s)	1704
BslFI	GGGAC	1 cut(s)	495
BslI	CCNNNNNNNGG	4 cut(s)	258, 347, 348, 563
BsmAI	GTCTC	4 cut(s)	182, 966, 1514, 1689
BsmBI	CGTCTC	2 cut(s)	182, 1689
BsmFI	GGGAC	1 cut(s)	495
BsnI	GGCC	2 cut(s)	288, 687
Bsp119I	TTCGAA	3 cut(s)	107, 1418, 1535
Bsp1286I	GDGCHC	3 cut(s)	753, 1307, 1363
Bsp143I	GATC	6 cut(s)	301, 422, 867, 1048, 1487, 1609
Bsp1720I	GCTNAGC	2 cut(s)	747, 843
Bsp19I	CCATGG	1 cut(s)	1170
Bsp68I	TCGCGA	1 cut(s)	1614
BspACI	CCGC	2 cut(s)	305, 847
BspANI	GGCC	2 cut(s)	288, 687
BspCNI	CTCAG	7 cut(s)	697, 739, 754, 765, 835, 871, 922
BspFNI	CGCG	1 cut(s)	1614
BspLI	GGNNCC	1 cut(s)	116
BspMAI	CTGCAG	1 cut(s)	1408
BspMI	ACCTGC	1 cut(s)	1619
BspPI	GGATC	4 cut(s)	296, 875, 1043, 1617
BspQI	GCTCTTC	1 cut(s)	109
BspT104I	TTCGAA	3 cut(s)	107, 1418, 1535
BspT107I	GGYRCC	1 cut(s)	114
BsrBI	CCGCTC	1 cut(s)	847
BsrDI	GCAATG	1 cut(s)	699
BsrI	ACTGG	2 cut(s)	568, 956
BssECI	CCNNGG	5 cut(s)	87, 810, 1170, 1595, 1604
BssMI	GATC	6 cut(s)	301, 422, 867, 1048, 1487, 1609
BssT1I	CCWWGG	2 cut(s)	1170, 1604
Bst2UI	CCWGG	1 cut(s)	285
Bst4CI	ACNGT	4 cut(s)	119, 137, 637, 1557
Bst6I	CTCTTC	5 cut(s)	109, 142, 188, 212, 849
BstAPI	GCANNNNNTGC	1 cut(s)	1412
BstBI	TTCGAA	3 cut(s)	107, 1418, 1535
BstC8I	GCNNGC	4 cut(s)	182, 393, 1280, 1528
BstDEI	CTNAG	9 cut(s)	705, 747, 752, 762, 843, 879, 930, 1371, 1384
BstDSI	CCRYGG	3 cut(s)	810, 1170, 1595
BstFNI	CGCG	1 cut(s)	1614
BstH2I	RGCGCY	1 cut(s)	103
BstHHI	GCGC	1 cut(s)	102
BstKTI	GATC	6 cut(s)	304, 425, 870, 1051, 1490, 1612
BstMAI	GTCTC	4 cut(s)	182, 966, 1514, 1689
BstMBI	GATC	6 cut(s)	301, 422, 867, 1048, 1487, 1609
BstMWI	GCNNNNNNNGC	8 cut(s)	304, 722, 815, 839, 1007, 1066, 1367, 1412
BstNI	CCWGG	1 cut(s)	285
BstNSI	RCATGY	1 cut(s)	1282
BstSCI	CCNGG	1 cut(s)	283
BstSFI	CTRYAG	1 cut(s)	1404
BstUI	CGCG	1 cut(s)	1614
BstV1I	GCAGC	7 cut(s)	728, 731, 734, 1151, 1233, 1390, 1418
BstX2I	RGATCY	2 cut(s)	422, 1048
BstYI	RGATCY	2 cut(s)	422, 1048
BsuRI	GGCC	2 cut(s)	288, 687
BtgI	CCRYGG	3 cut(s)	810, 1170, 1595
BtgZI	GCGATG	2 cut(s)	54, 258
BtrI	CACGTC	1 cut(s)	1319
BtuMI	TCGCGA	1 cut(s)	1614
BveI	ACCTGC	1 cut(s)	1619
Cac8I	GCNNGC	4 cut(s)	182, 393, 1280, 1528
CfoI	GCGC	1 cut(s)	102
Cfr13I	GGNCC	3 cut(s)	665, 686, 794
Csp6I	GTAC	4 cut(s)	638, 949, 1502, 1705
CviAII	CATG	9 cut(s)	646, 871, 1100, 1171, 1201, 1265, 1279, 1335, 1666
CviQI	GTAC	4 cut(s)	638, 949, 1502, 1705
DdeI	CTNAG	9 cut(s)	705, 747, 752, 762, 843, 879, 930, 1371, 1384
DpnI	GATC	6 cut(s)	303, 424, 869, 1050, 1489, 1611
DpnII	GATC	6 cut(s)	301, 422, 867, 1048, 1487, 1609
Eam1104I	CTCTTC	5 cut(s)	109, 142, 188, 212, 849
EarI	CTCTTC	5 cut(s)	109, 142, 188, 212, 849
Ecl136II	GAGCTC	1 cut(s)	751
Eco130I	CCWWGG	2 cut(s)	1170, 1604
Eco24I	GRGCYC	2 cut(s)	753, 1363
Eco47I	GGWCC	2 cut(s)	665, 794
Eco47III	AGCGCT	1 cut(s)	101
Eco53kI	GAGCTC	1 cut(s)	751
Eco57I	CTGAAG	2 cut(s)	461, 1335
EcoICRI	GAGCTC	1 cut(s)	751
EcoO109I	RGGNCCY	1 cut(s)	794
EcoRII	CCWGG	1 cut(s)	283
EcoT14I	CCWWGG	2 cut(s)	1170, 1604
EcoT22I	ATGCAT	1 cut(s)	647
EcoT38I	GRGCYC	2 cut(s)	753, 1363
ErhI	CCWWGG	2 cut(s)	1170, 1604
Esp3I	CGTCTC	2 cut(s)	182, 1689
FaeI	CATG	9 cut(s)	649, 874, 1103, 1174, 1204, 1268, 1282, 1338, 1669
FaqI	GGGAC	1 cut(s)	495
FatI	CATG	9 cut(s)	645, 870, 1099, 1170, 1200, 1264, 1278, 1334, 1665
Fnu4HI	GCNGC	7 cut(s)	717, 720, 723, 1140, 1247, 1404, 1407
FriOI	GRGCYC	2 cut(s)	753, 1363
Fsp4HI	GCNGC	7 cut(s)	717, 720, 723, 1140, 1247, 1404, 1407
FspBI	CTAG	2 cut(s)	1193, 1329
GlaI	GCGC	1 cut(s)	101
GluI	GCNGC	7 cut(s)	717, 720, 723, 1140, 1247, 1404, 1407
HaeII	RGCGCY	1 cut(s)	103
HaeIII	GGCC	2 cut(s)	288, 687
HapII	CCGG	2 cut(s)	252, 289
HhaI	GCGC	1 cut(s)	102
Hin1II	CATG	9 cut(s)	649, 874, 1103, 1174, 1204, 1268, 1282, 1338, 1669
Hin6I	GCGC	1 cut(s)	100
HinP1I	GCGC	1 cut(s)	100
HincII	GTYRAC	1 cut(s)	400
HindII	GTYRAC	1 cut(s)	400
HindIII	AAGCTT	1 cut(s)	1029
HpaII	CCGG	2 cut(s)	252, 289
HphI	GGTGA	3 cut(s)	995, 1190, 1334
Hpy166II	GTNNAC	2 cut(s)	400, 1465
Hpy188III	TCNNGA	5 cut(s)	144, 584, 977, 1052, 1613
Hpy8I	GTNNAC	2 cut(s)	400, 1465
Hpy99I	CGWCG	5 cut(s)	56, 59, 123, 137, 637
HpyCH4III	ACNGT	4 cut(s)	119, 137, 637, 1557
HpyCH4IV	ACGT	1 cut(s)	1318
HpyCH4V	TGCA	9 cut(s)	580, 645, 692, 716, 973, 1282, 1334, 1406, 1526
HpyF10VI	GCNNNNNNNGC	8 cut(s)	304, 722, 815, 839, 1007, 1066, 1367, 1412
HpyF3I	CTNAG	9 cut(s)	705, 747, 752, 762, 843, 879, 930, 1371, 1384
HpySE526I	ACGT	1 cut(s)	1318
Hsp92II	CATG	9 cut(s)	649, 874, 1103, 1174, 1204, 1268, 1282, 1338, 1669
HspAI	GCGC	1 cut(s)	100
Kzo9I	GATC	6 cut(s)	301, 422, 867, 1048, 1487, 1609
LguI	GCTCTTC	1 cut(s)	109
LmnI	GCTCC	2 cut(s)	89, 908
Lsp1109I	GCAGC	7 cut(s)	728, 731, 734, 1151, 1233, 1390, 1418
LweI	GCATC	7 cut(s)	445, 589, 632, 649, 865, 982, 1441
MaeI	CTAG	2 cut(s)	1193, 1329
MaeII	ACGT	1 cut(s)	1318
MaeIII	GTNAC	5 cut(s)	708, 859, 1155, 1379, 1557
MalI	GATC	6 cut(s)	303, 424, 869, 1050, 1489, 1611
MbiI	CCGCTC	1 cut(s)	847
MboI	GATC	6 cut(s)	301, 422, 867, 1048, 1487, 1609
MfeI	CAATTG	2 cut(s)	35, 681
MflI	RGATCY	2 cut(s)	422, 1048
MhlI	GDGCHC	3 cut(s)	753, 1307, 1363
MluCI	AATT	7 cut(s)	35, 323, 335, 411, 681, 728, 1106
MlyI	GAGTC	3 cut(s)	257, 301, 1663
MmeI	TCCRAC	2 cut(s)	747, 1632
Mph1103I	ATGCAT	1 cut(s)	647
MroXI	GAANNNNTTC	1 cut(s)	449
MseI	TTAA	7 cut(s)	432, 939, 1017, 1035, 1083, 1110, 1209
MspA1I	CMGCKG	1 cut(s)	704
MspI	CCGG	2 cut(s)	252, 289
MspR9I	CCNGG	1 cut(s)	285
MunI	CAATTG	2 cut(s)	35, 681
MvaI	CCWGG	1 cut(s)	285
MvnI	CGCG	1 cut(s)	1614
MwoI	GCNNNNNNNGC	8 cut(s)	304, 722, 815, 839, 1007, 1066, 1367, 1412
NcoI	CCATGG	1 cut(s)	1170
NdeII	GATC	6 cut(s)	301, 422, 867, 1048, 1487, 1609
NlaIII	CATG	9 cut(s)	649, 874, 1103, 1174, 1204, 1268, 1282, 1338, 1669
NlaIV	GGNNCC	1 cut(s)	116
NmuCI	GTSAC	2 cut(s)	859, 1557
NruI	TCGCGA	1 cut(s)	1614
NsiI	ATGCAT	1 cut(s)	647
NspI	RCATGY	1 cut(s)	1282
NspV	TTCGAA	3 cut(s)	107, 1418, 1535
PaeI	GCATGC	1 cut(s)	1282
PaqCI	CACCTGC	1 cut(s)	1619
PciSI	GCTCTTC	1 cut(s)	109
PdmI	GAANNNNTTC	1 cut(s)	449
PfeI	GAWTC	9 cut(s)	44, 59, 92, 275, 538, 776, 827, 1293, 1655
Pfl23II	CGTACG	1 cut(s)	1704
PkrI	GCNGC	7 cut(s)	718, 721, 724, 1141, 1248, 1405, 1408
PleI	GAGTC	3 cut(s)	256, 300, 1663
PpsI	GAGTC	3 cut(s)	256, 300, 1663
PpuMI	RGGWCCY	1 cut(s)	794
Psp124BI	GAGCTC	1 cut(s)	753
Psp5II	RGGWCCY	1 cut(s)	794
Psp6I	CCWGG	1 cut(s)	283
PspGI	CCWGG	1 cut(s)	283
PspLI	CGTACG	1 cut(s)	1704
PspN4I	GGNNCC	1 cut(s)	116
PspPI	GGNCC	3 cut(s)	665, 686, 794
PspPPI	RGGWCCY	1 cut(s)	794
PstI	CTGCAG	1 cut(s)	1408
PsuI	RGATCY	2 cut(s)	422, 1048
PvuII	CAGCTG	1 cut(s)	704
RruI	TCGCGA	1 cut(s)	1614
RsaI	GTAC	4 cut(s)	639, 950, 1503, 1706
RsaNI	GTAC	4 cut(s)	638, 949, 1502, 1705
SacI	GAGCTC	1 cut(s)	753
SapI	GCTCTTC	1 cut(s)	109
SaqAI	TTAA	7 cut(s)	432, 939, 1017, 1035, 1083, 1110, 1209
SatI	GCNGC	7 cut(s)	717, 720, 723, 1140, 1247, 1404, 1407
Sau3AI	GATC	6 cut(s)	301, 422, 867, 1048, 1487, 1609
Sau96I	GGNCC	3 cut(s)	665, 686, 794
SchI	GAGTC	3 cut(s)	257, 301, 1663
ScrFI	CCNGG	1 cut(s)	285
SduI	GDGCHC	3 cut(s)	753, 1307, 1363
SfaNI	GCATC	7 cut(s)	445, 589, 632, 649, 865, 982, 1441
SfcI	CTRYAG	1 cut(s)	1404
SfuI	TTCGAA	3 cut(s)	107, 1418, 1535
SinI	GGWCC	2 cut(s)	665, 794
SmlI	CTYRAG	4 cut(s)	79, 996, 1088, 1671
SmoI	CTYRAG	4 cut(s)	79, 996, 1088, 1671
SpeI	ACTAGT	1 cut(s)	1328
SphI	GCATGC	1 cut(s)	1282
Sse9I	AATT	7 cut(s)	35, 323, 335, 411, 681, 728, 1106
SsiI	CCGC	2 cut(s)	305, 847
SspI	AATATT	2 cut(s)	785, 937
SspMI	CTAG	2 cut(s)	1193, 1329
SstI	GAGCTC	1 cut(s)	753
StyD4I	CCNGG	1 cut(s)	283
StyI	CCWWGG	2 cut(s)	1170, 1604
TaaI	ACNGT	4 cut(s)	119, 137, 637, 1557
TaiI	ACGT	1 cut(s)	1321
TaqI	TCGA	6 cut(s)	107, 143, 300, 333, 1418, 1535
TasI	AATT	7 cut(s)	35, 323, 335, 411, 681, 728, 1106
TfiI	GAWTC	9 cut(s)	44, 59, 92, 275, 538, 776, 827, 1293, 1655
Tru1I	TTAA	7 cut(s)	432, 939, 1017, 1035, 1083, 1110, 1209
Tru9I	TTAA	7 cut(s)	432, 939, 1017, 1035, 1083, 1110, 1209
TseFI	GTSAC	2 cut(s)	859, 1557
TseI	GCWGC	7 cut(s)	716, 719, 722, 1139, 1246, 1403, 1406
Tsp45I	GTSAC	2 cut(s)	859, 1557
TspDTI	ATGAA	9 cut(s)	51, 57, 319, 447, 636, 852, 1116, 1200, 1683
TspGWI	ACGGA	4 cut(s)	175, 187, 749, 1584
VpaK11BI	GGWCC	2 cut(s)	665, 794
XapI	RAATTY	3 cut(s)	335, 411, 1106
XceI	RCATGY	1 cut(s)	1282
XmnI	GAANNNNTTC	1 cut(s)	449
XspI	CTAG	2 cut(s)	1193, 1329
Zsp2I	ATGCAT	1 cut(s)	647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.