Prupe.8G058000_v2.0.a1

Protein of unknown function (DUF3752)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001274
Physical Location & Seq
Forward (+)
7274045 .. 7286921
12877 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G058000.4

Sequence Viewer

Length: 1737 bp
ATGGGCAGCAAAAGCAAGAGAAAGACGCATTTCGATGATACAAGCGATGACGATTCGTCATTGTCGTCTTCTTCAAGTTCTGAGGAATCTGAACGCTCTTCAAAGAGGCACCGGCGCCACCGCCACAGAAAAGACGATAGTTCGAGAAGAGAGAAAGAAAGGAGAAGGGAAAGGAGAGAGAAACGGAGAAGCAAGAGAGAGACGAGGAGAAGGAAATCCAAAAAGGAGGACAATAAGAAGAGGGCTTATGGGGTTGATGATGACTCTGGTTCTGGGGGGTCTTTAAGTGGTGATGATTCAGACCCAGGTAGGAGTCGGGCTGAACCGCAAGCCATTTTGCAAGGCTTGTTCAACGAATTTCCCAATGTTGGGAATGATTTGAAACAGCTTTTAGAAATGATTGATGGTGGGCAAGCAGTTGACATCAAGGGAATATCAGAAAGAACTTTGATTAAGCATCTAAGGAAGTTGTTCCTTGCATTGAACCTGAAGCAAAATGATGGAGTTTTTTTGCTGCCTTCAAATGTTCGTCCCACTTTGGAGGTTGTTGGGCTCATGATTCAAACATCCTTAGAACCTAAATCTGGACTGAATGAGATGCATCCAAAACAACTGGATGAGGAACACAGACAAGTGGCTGGTGAAAGTAATAGGACAGTGCCATGCATGGAGGATGATGATACTGGTCCCAAAAGAAGGGTAATTGGCCCTGCGATGCCATCAGCTGAGTTACTTGCTGCTGCAGCGAAATTGACAGATGCACAGGCTGAGCTCAGAGAAGCCGAGTTGGAAGAAGATAGTGAATTTTTTATAGGACCTCCACCGCCTGCTATGGTTGCTGAAACTGCTTCAGCTAATGAAGCTGAGCGGTTCGAAGAGGTTACAAGGATCATGGATGCTGCAGATGATAGTCTATATGATATTCTAGGAGCAAACCAAAATATGTCTGCTGAGAATATAAAGAAAAGGTATTGGAAGATGTCTCTTTTGGTGCATCCTGATAAATGCTCGCATCCTCAAGCTCAACAAGCATTTGTTAAGTTGAATAAAGCTTTTAAAGAATTACAAGATCCAGATAAGCGAAAAGTGCTGGATGACAAGATTAAACTCAAGGAGGAACATGAAAGATTTAAGGTTGAGTTGAAAGCAATGCGTGAGGCTGCACAGTGGAGAAAGTTACAAGGAATATCTATGGAGGGTGATGATGAGCTGCTAGCAGACATGGATGTTAAGGTGGCACCAAAAAGAGATGAGTGGATGACAACGCTGCCACCTGAAAGGAAACATGGTATGCCTCCTACACAATCAACCAGATTCAACAAGAGCACTAAAGAAGGACGCGGTAATACCAGTGCTTGGACTGATACCCCTTCAGACAGAGCCCAGAAAGCTAAGATGGGTTACTTGGAGGCGTATAATGAGGCTGCAGCCCTTGCTTCAAATGAGGAGGAAAAGAAAAAGCAGAGTTCAGATGCAGAATTGGTGGACAAATACAATAAAGAAAAACGATCAAAATCATTGGTGCAAAAACACAAAGAGGAGACTGCAAAGCGTTCAAAGAGAAAATTCAAGCAACAGACAGAGGAGGACTGGGTGGGGAAACATCCGTGGAAGCCTTGGGATCGTGAGAAGGATTTGTCAGGTGGGAGGCAAAGTGTAAAATTGGATTCAGAAAACATGAATCAAGCTTTAACGTCACGGTTTTCTTCAGGCTCATTTGAGAGAAACTTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001664 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0050780
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3752 DnaJ

Protein Analysis

579

Amino Acids

65.95

Weight (kDa)

8.91

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 108, 114, 1237
AccB7I CCANNNNNTGG 1 cut(s) 1356
AccBSI CCGCTC 1 cut(s) 868
AccII CGCG 1 cut(s) 1341
AciI CCGC 5 cut(s) 121, 326, 824, 868, 1341
AclWI GGATC 3 cut(s) 896, 1064, 1629
AcsI RAATTY 3 cut(s) 356, 803, 1565
AcuI CTGAAG 4 cut(s) 509, 834, 1356, 1692
AcyI GRCGYC 1 cut(s) 115
AfiI CCNNNNNNNGG 5 cut(s) 368, 369, 584, 696, 1356
AhdI GACNNNNNGTC 1 cut(s) 55
AjnI CCWGG 1 cut(s) 304
Alw21I GWGCWC 2 cut(s) 774, 1328
Alw26I GTCTC 3 cut(s) 194, 987, 1535
AlwI GGATC 3 cut(s) 896, 1064, 1629
AoxI GGCC 1 cut(s) 706
ApoI RAATTY 3 cut(s) 356, 803, 1565
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 3 cut(s) 686, 707, 815
AsuHPI GGTGA 3 cut(s) 302, 653, 1211
AsuII TTCGAA 1 cut(s) 873
AsuNHI GCTAGC 1 cut(s) 1213
AvaII GGWCC 2 cut(s) 686, 815
BanI GGYRCC 3 cut(s) 108, 114, 1237
BanII GRGCYC 3 cut(s) 555, 774, 1384
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 774, 1328
BccI CCATC 4 cut(s) 398, 494, 727, 1390
BciT130I CCWGG 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 194, 987, 1535
BfaI CTAG 2 cut(s) 926, 1214
BfmI CTRYAG 3 cut(s) 741, 900, 1425
BfoI RGCGCY 1 cut(s) 118
BlpI GCTNAGC 2 cut(s) 768, 864
Bme1390I CCNGG 1 cut(s) 306
Bme18I GGWCC 2 cut(s) 686, 815
BmeRI GACNNNNNGTC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 686, 707, 815
BmiI GGNNCC 4 cut(s) 110, 116, 688, 1239
BmrFI CCNGG 1 cut(s) 306
BmrI ACTGGG 1 cut(s) 1600
BmsI GCATC 9 cut(s) 466, 588, 610, 705, 748, 886, 1003, 1021, 1462
BmtI GCTAGC 1 cut(s) 1217
BmuI ACTGGG 1 cut(s) 1600
BpiI GAAGAC 1 cut(s) 60
Bpu1102I GCTNAGC 2 cut(s) 768, 864
Bpu14I TTCGAA 1 cut(s) 873
BpuEI CTTGAG 2 cut(s) 1002, 1094
BsaBI GATNNNNATC 1 cut(s) 1513
BsaHI GRCGYC 1 cut(s) 115
BsaJI CCNNGG 3 cut(s) 304, 1607, 1616
BsaXI ACNNNNNCTCC 2 cut(s) 1639, 1669
Bsc4I CCNNNNNNNGG 5 cut(s) 368, 369, 584, 696, 1356
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 4 cut(s) 618, 688, 1350, 1595
Bse3DI GCAATG 1 cut(s) 1155
Bse8I GATNNNNATC 1 cut(s) 1513
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 3 cut(s) 304, 1607, 1616
BseJI GATNNNNATC 1 cut(s) 1513
BseLI CCNNNNNNNGG 5 cut(s) 368, 369, 584, 696, 1356
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 6 cut(s) 72, 717, 759, 787, 855, 942
BseNI ACTGG 4 cut(s) 618, 688, 1350, 1595
BseRI GAGGAG 4 cut(s) 220, 1460, 1553, 1598
BsgI GTGCAG 1 cut(s) 1146
Bsh1236I CGCG 1 cut(s) 1341
BshFI GGCC 1 cut(s) 708
BshNI GGYRCC 3 cut(s) 108, 114, 1237
BsiHKAI GWGCWC 2 cut(s) 774, 1328
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 2 cut(s) 516, 672
BslI CCNNNNNNNGG 5 cut(s) 368, 369, 584, 696, 1356
BsmAI GTCTC 3 cut(s) 194, 987, 1535
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 2 cut(s) 516, 672
BsnI GGCC 1 cut(s) 708
Bsp119I TTCGAA 1 cut(s) 873
Bsp1286I GDGCHC 4 cut(s) 555, 774, 1328, 1384
Bsp143I GATC 4 cut(s) 888, 1069, 1508, 1621
Bsp1720I GCTNAGC 2 cut(s) 768, 864
BspACI CCGC 5 cut(s) 121, 326, 824, 868, 1341
BspANI GGCC 1 cut(s) 708
BspCNI CTCAG 6 cut(s) 73, 718, 760, 786, 856, 943
BspFNI CGCG 1 cut(s) 1341
BspHI TCATGA 1 cut(s) 555
BspLI GGNNCC 4 cut(s) 110, 116, 688, 1239
BspMAI CTGCAG 3 cut(s) 745, 904, 1429
BspOI GCTAGC 1 cut(s) 1217
BspPI GGATC 3 cut(s) 896, 1064, 1629
BspQI GCTCTTC 1 cut(s) 103
BspT104I TTCGAA 1 cut(s) 873
BspT107I GGYRCC 3 cut(s) 108, 114, 1237
BsrBI CCGCTC 1 cut(s) 868
BsrDI GCAATG 1 cut(s) 1155
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 4 cut(s) 618, 688, 1350, 1595
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 3 cut(s) 304, 1607, 1616
BssMI GATC 4 cut(s) 888, 1069, 1508, 1621
BssNI GRCGYC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 1616
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 3 cut(s) 658, 1167, 1701
Bst6I CTCTTC 4 cut(s) 103, 142, 233, 870
BstACI GRCGYC 1 cut(s) 115
BstAPI GCANNNNNTGC 1 cut(s) 1433
BstBI TTCGAA 1 cut(s) 873
BstC8I GCNNGC 5 cut(s) 330, 414, 828, 1010, 1215
BstDEI CTNAG 9 cut(s) 81, 461, 571, 726, 768, 773, 864, 951, 1392
BstDSI CCRYGG 1 cut(s) 1607
BstFNI CGCG 1 cut(s) 1341
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 4 cut(s) 891, 1072, 1511, 1624
BstMAI GTCTC 3 cut(s) 194, 987, 1535
BstMBI GATC 4 cut(s) 888, 1069, 1508, 1621
BstMWI GCNNNNNNNGC 9 cut(s) 12, 743, 836, 845, 860, 1028, 1087, 1388, 1433
BstNI CCWGG 1 cut(s) 306
BstSCI CCNGG 1 cut(s) 304
BstSFI CTRYAG 3 cut(s) 741, 900, 1425
BstUI CGCG 1 cut(s) 1341
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 1069
BsuRI GGCC 1 cut(s) 708
BtgI CCRYGG 1 cut(s) 1607
BtgZI GCGATG 2 cut(s) 60, 728
BtsIMutI CAGTG 3 cut(s) 663, 1172, 1357
Cac8I GCNNGC 5 cut(s) 330, 414, 828, 1010, 1215
CciI TCATGA 1 cut(s) 555
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 111
Cfr13I GGNCC 3 cut(s) 686, 707, 815
CseI GACGC 2 cut(s) 34, 1347
CviAII CATG 8 cut(s) 556, 663, 667, 892, 1121, 1222, 1286, 1678
DdeI CTNAG 9 cut(s) 81, 461, 571, 726, 768, 773, 864, 951, 1392
DinI GGCGCC 1 cut(s) 116
DpnI GATC 4 cut(s) 890, 1071, 1510, 1623
DpnII GATC 4 cut(s) 888, 1069, 1508, 1621
DraI TTTAAA 1 cut(s) 1057
DriI GACNNNNNGTC 1 cut(s) 55
Eam1104I CTCTTC 4 cut(s) 103, 142, 233, 870
Eam1105I GACNNNNNGTC 1 cut(s) 55
EarI CTCTTC 4 cut(s) 103, 142, 233, 870
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 1 cut(s) 1616
Eco24I GRGCYC 3 cut(s) 555, 774, 1384
Eco47I GGWCC 2 cut(s) 686, 815
Eco53kI GAGCTC 1 cut(s) 772
Eco57I CTGAAG 4 cut(s) 509, 834, 1356, 1692
EcoICRI GAGCTC 1 cut(s) 772
EcoO109I RGGNCCY 1 cut(s) 815
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 1616
EcoT22I ATGCAT 2 cut(s) 603, 668
EcoT38I GRGCYC 3 cut(s) 555, 774, 1384
EgeI GGCGCC 1 cut(s) 116
EheI GGCGCC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 1616
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 8 cut(s) 559, 666, 670, 895, 1124, 1225, 1289, 1681
FaqI GGGAC 2 cut(s) 516, 672
FatI CATG 8 cut(s) 555, 662, 666, 891, 1120, 1221, 1285, 1677
FriOI GRGCYC 3 cut(s) 555, 774, 1384
FspBI CTAG 2 cut(s) 926, 1214
GlaI GCGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 708
HapII CCGG 1 cut(s) 112
HgaI GACGC 2 cut(s) 34, 1347
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 8 cut(s) 559, 666, 670, 895, 1124, 1225, 1289, 1681
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 2 cut(s) 1050, 1686
HinfI GANTC 9 cut(s) 53, 86, 263, 296, 313, 559, 1314, 1667, 1681
HpaII CCGG 1 cut(s) 112
HphI GGTGA 3 cut(s) 302, 653, 1211
Hpy166II GTNNAC 2 cut(s) 421, 1486
Hpy188I TCNGA 8 cut(s) 82, 91, 301, 439, 776, 1375, 1471, 1672
Hpy188III TCNNGA 6 cut(s) 144, 556, 585, 998, 1073, 1625
Hpy8I GTNNAC 2 cut(s) 421, 1486
HpyAV CCTTC 7 cut(s) 159, 204, 528, 690, 1328, 1380, 1624
HpyCH4III ACNGT 3 cut(s) 658, 1167, 1701
HpyCH4IV ACGT 1 cut(s) 1694
HpyF10VI GCNNNNNNNGC 9 cut(s) 12, 743, 836, 845, 860, 1028, 1087, 1388, 1433
HpyF3I CTNAG 9 cut(s) 81, 461, 571, 726, 768, 773, 864, 951, 1392
HpySE526I ACGT 1 cut(s) 1694
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 8 cut(s) 559, 666, 670, 895, 1124, 1225, 1289, 1681
HspAI GCGC 1 cut(s) 115
KasI GGCGCC 1 cut(s) 114
Kzo9I GATC 4 cut(s) 888, 1069, 1508, 1621
LguI GCTCTTC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 929
LweI GCATC 9 cut(s) 466, 588, 610, 705, 748, 886, 1003, 1021, 1462
MaeI CTAG 2 cut(s) 926, 1214
MaeII ACGT 1 cut(s) 1694
MaeIII GTNAC 5 cut(s) 729, 880, 1176, 1400, 1695
MalI GATC 4 cut(s) 890, 1071, 1510, 1623
MbiI CCGCTC 1 cut(s) 868
MboI GATC 4 cut(s) 888, 1069, 1508, 1621
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 4 cut(s) 555, 774, 1328, 1384
MluCI AATT 8 cut(s) 356, 702, 749, 803, 1061, 1478, 1565, 1661
Mly113I GGCGCC 1 cut(s) 115
MlyI GAGTC 2 cut(s) 257, 322
MmeI TCCRAC 1 cut(s) 768
Mph1103I ATGCAT 2 cut(s) 603, 668
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 8 cut(s) 284, 453, 1038, 1056, 1104, 1131, 1230, 1691
MslI CAYNNNNRTG 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 306
MvnI CGCG 1 cut(s) 1341
MwoI GCNNNNNNNGC 9 cut(s) 12, 743, 836, 845, 860, 1028, 1087, 1388, 1433
NarI GGCGCC 1 cut(s) 115
NdeII GATC 4 cut(s) 888, 1069, 1508, 1621
NheI GCTAGC 1 cut(s) 1213
NlaIII CATG 8 cut(s) 559, 666, 670, 895, 1124, 1225, 1289, 1681
NlaIV GGNNCC 4 cut(s) 110, 116, 688, 1239
NmeAIII GCCGAG 1 cut(s) 808
NmuCI GTSAC 1 cut(s) 1695
NsiI ATGCAT 2 cut(s) 603, 668
NspV TTCGAA 1 cut(s) 873
PagI TCATGA 1 cut(s) 555
PciSI GCTCTTC 1 cut(s) 103
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 7 cut(s) 53, 86, 296, 559, 1314, 1667, 1681
PflMI CCANNNNNTGG 1 cut(s) 1356
PleI GAGTC 2 cut(s) 257, 321
PluTI GGCGCC 1 cut(s) 118
PpsI GAGTC 2 cut(s) 257, 321
PpuMI RGGWCCY 1 cut(s) 815
Psp124BI GAGCTC 1 cut(s) 774
Psp5II RGGWCCY 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 4 cut(s) 110, 116, 688, 1239
PspPI GGNCC 3 cut(s) 686, 707, 815
PspPPI RGGWCCY 1 cut(s) 815
PstI CTGCAG 3 cut(s) 745, 904, 1429
PsuI RGATCY 1 cut(s) 1069
PvuII CAGCTG 1 cut(s) 725
RseI CAYNNNNRTG 1 cut(s) 33
SacI GAGCTC 1 cut(s) 774
SapI GCTCTTC 1 cut(s) 103
SaqAI TTAA 8 cut(s) 284, 453, 1038, 1056, 1104, 1131, 1230, 1691
Sau3AI GATC 4 cut(s) 888, 1069, 1508, 1621
Sau96I GGNCC 3 cut(s) 686, 707, 815
SchI GAGTC 2 cut(s) 257, 322
ScrFI CCNGG 1 cut(s) 306
SduI GDGCHC 4 cut(s) 555, 774, 1328, 1384
SfaNI GCATC 9 cut(s) 466, 588, 610, 705, 748, 886, 1003, 1021, 1462
SfcI CTRYAG 3 cut(s) 741, 900, 1425
SfoI GGCGCC 1 cut(s) 116
SfuI TTCGAA 1 cut(s) 873
SgrAI CRCCGGYG 1 cut(s) 111
SinI GGWCC 2 cut(s) 686, 815
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 2 cut(s) 1017, 1109
SmoI CTYRAG 2 cut(s) 1017, 1109
Sse9I AATT 8 cut(s) 356, 702, 749, 803, 1061, 1478, 1565, 1661
SsiI CCGC 5 cut(s) 121, 326, 824, 868, 1341
SspDI GGCGCC 1 cut(s) 114
SspMI CTAG 2 cut(s) 926, 1214
SstI GAGCTC 1 cut(s) 774
StyD4I CCNGG 1 cut(s) 304
StyI CCWWGG 1 cut(s) 1616
TaaI ACNGT 3 cut(s) 658, 1167, 1701
TaiI ACGT 1 cut(s) 1697
TaqI TCGA 3 cut(s) 33, 143, 873
TasI AATT 8 cut(s) 356, 702, 749, 803, 1061, 1478, 1565, 1661
TfiI GAWTC 7 cut(s) 53, 86, 296, 559, 1314, 1667, 1681
Tru1I TTAA 8 cut(s) 284, 453, 1038, 1056, 1104, 1131, 1230, 1691
Tru9I TTAA 8 cut(s) 284, 453, 1038, 1056, 1104, 1131, 1230, 1691
TscAI CASTG 3 cut(s) 663, 1172, 1357
TseFI GTSAC 1 cut(s) 1695
Tsp45I GTSAC 1 cut(s) 1695
TspDTI ATGAA 3 cut(s) 873, 1137, 1694
TspGWI ACGGA 2 cut(s) 199, 1596
TspRI CASTG 3 cut(s) 663, 1172, 1357
Van91I CCANNNNNTGG 1 cut(s) 1356
VpaK11BI GGWCC 2 cut(s) 686, 815
XapI RAATTY 3 cut(s) 356, 803, 1565
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 2 cut(s) 926, 1214
Zsp2I ATGCAT 2 cut(s) 603, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.