FvH4_4g16700

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000725
Physical Location & Seq
Forward (+)
20558427 .. 20562072
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16700.t1

Sequence Viewer

Length: 3003 bp
ATGGTTGAAGCAGTTCCATTTGGTACTGCCGCAGACATACTCATCAAGCTGGGGTCTTCCACTTTCCAGAAATTCGGGCTCATCTACGGCGTCAACAAGGAAATAAAGAAGCTCGAGAACACCCTCTCCACCATCAAAGCTGTGCTCTTGGATGCAGAGGAGAAGCAAGAGAAGAGCCATCTGGTGCAGGACTGGATCAGGAAGCTCAACGAGGTTGTGTATGAGGCAGATGACTTGTTGGATGCTTTCGAAACCAAAGCAAGGCAGCTTGAGAATCATCAGGTGCGTGAGTTCTTTTCAAAGACACTTGCTTTTAAGTACAAGATGAGTCGAAAGTTGAGAGAAACTAGAGAGAGATTGGATGAGATTGCAGGGGATATTTCAAAGTTTAACTTCAGGGACAGGTCATTGATAATATCAGATATTGGGGTTGAGATCAATAATAGGAGAGAAGAGACGCATTCTTTTATGGTGGAATCAGCTGTGGTTGGAAGAAAGAGAGAGAAGGAGGAAATGGTGGAGTTCTTGACGAACCCACCAAGTGAAGGAGAAAATGTTTATGTTACTGCCATTGTTGGTATTGGGGGACTTGGGAAGACGACCCTGGCTAAAATGTTGTATAATGATGATAGGGTAGGCAACTATTTTGAATTTAAGATGTGGGTGTGGGGTTATGGAGACTTTAATTTATATTCAATTGCTGAGAAAATCTTGAGATCTAGTGCTACGAATGAACTAGAGGATTCGGAGTTTTATCACCAGCATATTTTGATGCAGAAGAAACTGTTGATGGAGGAGTTGCAGATTCTTCTTCGGAAGAAATTGGATGGGAAGCGCTACCTTCTTGTTCTGGATGATGTGTGGAATGTGAACCGAGAAAGCTGGCTTCAGCTGAATGATTTGTTGAAAGGTGGTGCCAATGGGACTAAGGTTGTTGTGACAACTCGTAGCACGAAAATTACTTCGCTTATGGATATCGATTCCCCTTTTGTGCTACATGATCTATCGGAGGAAGAGAGTTGGACATTGTTCAAGCAACTGGCTTTTAAAGGTGGAAGGGAGGAGGAGCATCCGGAGTTGGTGCCAATAGGAAGAAGAATTGTGAAGAAATGCGGAGGTGTTCCGCTTGCAATAAGGACTCTAGGAAGCATGATGAGATATAAAGCTAATGAAATCGAGTGGATTGCGATTCAAAATACAGAGATTTGGGAGTTACCAAAAGAGGAGAATGATGTTCTGAAGATACTCAAGTTAAGCTATGATCACATGCCAATTTATTTGAAACAATGCTTCTCCTACTGTGCATTGTTCCCTAAGGGATATGCAATTGAGAAGAAAATGTTAGTGGAACTTTGGATAGCTCAGGGTTACATTCACCTTCCTGGTAAAGAGAGGAACTTAGAAGAAATTGGTGACATGTATTTCAAGGAATTGATAGAGAGATCATTATTTCAAGAGGTCAAGAAAGATGCCAATGGAATTTTAATTAGTTGTCATATGCATGACCTCATACACGATCTTTCTCGGGCAGTTGCAGGATCTGAAGTCTCAGTTTGCAGTGTAGATGTTGGAGATATATCTGAAAGAGTTCATCATCTTTCATTTAGTTGTTATCTGCCTTCTTCATGGGAAGTTCCGATAGCATTGCTCAAAGCAAAGAAGACTAGAACATTTATGCTACCACTTCAGGATCAAGCATATAAAATTTCCAGTCACAATTCAATCATTTCAAACTTGAGGTGCTTACGTGTGTTAGATCTGCATAACCTAAGAATCAATGAGCTTTCAAATTCCGTAGGCAAGTTGACCCATCTTAGGTACCTTGACCTCTCTGGTAATGTTAGTATGACCGCACTCCCTGCTTCTATAAGCAATCTGCAGAATCTGCAGACGCTTAAACTCAACAGGTGCTACAGACTTGAACAACTACCTAGAGATATTGGAAAGATGACCAGCCTTAGACATCTTGAGATCACTGGGTGCCGTAAATTGAATCACATGCCACTTGGGATGGGAAACTTGGTATCTCTTCAGACATTACCGTTATTCATCTTGGGGAAGAAAGATCTCTTTTCCGGGGTTAGTGGTGGACTAGATGAGTTGAGTGGCCTGAACTGTCTTAAAGGGGTACTTCATGTTGTCCATCTTGAACATGTCGACAATGGTACAATGGATTCAAAGAGTTCAAATTTGAGCAGCAAGCAGTACCTAGAATCCTTAAAACTAATTTGGTCCAGAGACGACAACAGTGGAAATAATGAAGATGACATGGTGTTGGAAGGCCTCCAACCCCATCAAAATTTGAAAGTGCTCCATATAGCTGGGTATTGCGGCGTTCACTTCCCCAACTGGCTGATGAACAATGCTGCTTCCTCTCTTCCAAGTTTAGTGGAGCTCACAATTGAGGGCTGTCTCAGATGCCAGCATCTTCCACCACTTGATCAACTCCCTTCTCTCAAGTTTCTCAAACTGTATTCTCTAAGTTCCCTGGAGCACATCAACAACAGTTACTCATCTTCCTCTTCTAGTTTGGCTGGTGAAGGAAGAGAGCCTTTGTTTTTTCCATCATTGAAAGAGCTCACACTTTATGACTTGCCACTCTTGAAGCTGTGGCAAACATGCAGTGATGAAGATGTGACAACAACAATGCCAAATCGACAGCTTTCATTTCCTTCCCTCACCAAATTGACAATCGTGGACGCCCCTAACTTAACATCCATGCCAATGCTTCCACTAGTTGAAGAATTGCTTATGGACACCATCTCGACAAAGCTGTTGCAGTCAATGTTGATGGCAGCAGCCTCAGAAAGACATACAAGCTCTTCCTCCCTGGCTAGATTGAAATTTTTGATCATCAAATCTTGTAATGATCTTGTGTCTTTCCCTGAAGATAGGATAGCCAGCCTCACTTCCCTTAAGCTTCTAAAGATTATAGATTGTGGAAACTTGACATCTCTACCACAGAACCTGCACTGTGAATACGTACTCAGTAATTCCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1001

Amino Acids

113.54

Weight (kDa)

6.27

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 14 - 96 3.9e-22 Rx N-terminal domain
NB-ARC PF00931 170 - 350 3.3e-30 NB-ARC domain
WHD_DRP PF23559 436 - 507 4.8e-25 Disease resistance protein Winged helix domain
LRR_14 PF23598 575 - 863 6e-34 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 698 - 826 6.6e-35 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2976
Acc65I GGTACC 1 cut(s) 1818
AccB1I GGYRCC 4 cut(s) 914, 1081, 1818, 1980
AccI GTMKAC 1 cut(s) 2157
AccIII TCCGGA 1 cut(s) 1072
AciI CCGC 5 cut(s) 30, 1113, 1124, 1851, 2331
AclWI GGATC 3 cut(s) 203, 1546, 1698
AcsI RAATTY 8 cut(s) 71, 650, 1479, 1704, 1789, 2188, 2299, 2843
AcuI CTGAAG 7 cut(s) 379, 872, 1259, 1563, 1670, 2015, 2907
AcyI GRCGYC 2 cut(s) 90, 2700
AdeI CACNNNGTG 2 cut(s) 542, 1980
AfaI GTAC 7 cut(s) 25, 320, 1820, 2130, 2167, 2207, 2985
AfeI AGCGCT 1 cut(s) 836
AfiI CCNNNNNNNGG 3 cut(s) 261, 545, 1815
AflII CTTAAG 1 cut(s) 2915
AflIII ACRYGT 3 cut(s) 1416, 1747, 2152
AhlI ACTAGT 1 cut(s) 2734
AjnI CCWGG 5 cut(s) 603, 1381, 2487, 2829, 2996
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
Alw21I GWGCWC 5 cut(s) 147, 2313, 2397, 2496, 2580
Alw26I GTCTC 5 cut(s) 449, 672, 1552, 2232, 2417
AlwI GGATC 3 cut(s) 203, 1546, 1698
AlwNI CAGNNNCTG 3 cut(s) 1541, 1885, 2968
Ama87I CYCGRG 2 cut(s) 113, 1524
Aor13HI TCCGGA 1 cut(s) 1072
Aor51HI AGCGCT 1 cut(s) 836
AoxI GGCC 2 cut(s) 2107, 2281
ApeKI GCWGC 5 cut(s) 265, 2196, 2366, 2795, 2798
ApoI RAATTY 8 cut(s) 71, 650, 1479, 1704, 1789, 2188, 2299, 2843
Asp700I GAANNNNTTC 2 cut(s) 12, 1587
Asp718I GGTACC 1 cut(s) 1818
AspLEI GCGC 1 cut(s) 837
AspS9I GGNCC 1 cut(s) 2232
AsuC2I CCSGG 1 cut(s) 2077
AsuHPI GGTGA 5 cut(s) 749, 1367, 1424, 2549, 2671
AsuII TTCGAA 1 cut(s) 249
AvaI CYCGRG 2 cut(s) 113, 1524
AvaII GGWCC 1 cut(s) 2232
AxyI CCTNAGG 1 cut(s) 1314
BaeI ACNNNNGTAYC 2 cut(s) 1235, 1268
BanI GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BanII GRGCYC 3 cut(s) 81, 2397, 2580
BbsI GAAGAC 3 cut(s) 48, 602, 1667
Bbv12I GWGCWC 5 cut(s) 147, 2313, 2397, 2496, 2580
BbvI GCAGC 5 cut(s) 277, 2208, 2353, 2807, 2810
BceAI ACGGC 2 cut(s) 103, 1968
BcgI CGANNNNNNTGC 4 cut(s) 1166, 1200, 1627, 1661
BciT130I CCWGG 5 cut(s) 605, 1383, 2489, 2831, 2998
BclI TGATCA 3 cut(s) 1261, 2440, 2850
BcnI CCSGG 1 cut(s) 2077
BcoDI GTCTC 5 cut(s) 449, 672, 1552, 2232, 2417
BcuI ACTAGT 1 cut(s) 2734
BfmI CTRYAG 3 cut(s) 1877, 1886, 1912
BfoI RGCGCY 1 cut(s) 838
BfrI CTTAAG 1 cut(s) 2915
BfuAI ACCTGC 1 cut(s) 2976
BglII AGATCT 3 cut(s) 716, 1756, 2065
BisI GCNGC 7 cut(s) 30, 266, 2197, 2332, 2367, 2796, 2799
BlsI GCNGC 7 cut(s) 31, 267, 2198, 2333, 2368, 2797, 2800
Bme1390I CCNGG 6 cut(s) 605, 1383, 2077, 2489, 2831, 2998
Bme18I GGWCC 1 cut(s) 2232
BmeT110I CYCGRG 2 cut(s) 113, 1524
BmgT120I GGNCC 1 cut(s) 2232
BmiI GGNNCC 4 cut(s) 916, 1083, 1820, 1982
BmrFI CCNGG 6 cut(s) 605, 1383, 2077, 2489, 2831, 2998
BmrI ACTGGG 1 cut(s) 1986
BmsI GCATC 7 cut(s) 142, 232, 762, 1078, 1459, 2408, 2434
BmuI ACTGGG 1 cut(s) 1986
BpiI GAAGAC 3 cut(s) 48, 602, 1667
BpmI CTGGAG 1 cut(s) 2510
Bpu10I CCTNAGC 1 cut(s) 1362
Bpu14I TTCGAA 1 cut(s) 249
BpuEI CTTGAG 6 cut(s) 290, 733, 1232, 1756, 1988, 2441
BpuMI CCSGG 1 cut(s) 2077
Bsa29I ATCGAT 1 cut(s) 978
BsaAI YACGTR 2 cut(s) 1748, 2983
BsaHI GRCGYC 2 cut(s) 90, 2700
BsaJI CCNNGG 4 cut(s) 603, 2076, 2487, 2829
BsaWI WCCGGW 1 cut(s) 1072
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 540, 570
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 545, 1815
Bse1I ACTGG 5 cut(s) 197, 1044, 1710, 1981, 2354
Bse21I CCTNAGG 1 cut(s) 1314
Bse3DI GCAATG 1 cut(s) 1643
BseAI TCCGGA 1 cut(s) 1072
BseBI CCWGG 5 cut(s) 605, 1383, 2489, 2831, 2998
BseCI ATCGAT 1 cut(s) 978
BseDI CCNNGG 4 cut(s) 603, 2076, 2487, 2829
BseGI GGATG 8 cut(s) 157, 247, 367, 832, 859, 1069, 2016, 2714
BseLI CCNNNNNNNGG 3 cut(s) 261, 545, 1815
BseMI GCAATG 1 cut(s) 1643
BseMII CTCAG 6 cut(s) 693, 1376, 1563, 2428, 2817, 3001
BseNI ACTGG 5 cut(s) 197, 1044, 1710, 1981, 2354
BseRI GAGGAG 5 cut(s) 173, 809, 1076, 1079, 1238
BseXI GCAGC 5 cut(s) 277, 2208, 2353, 2807, 2810
BseYI CCCAGC 2 cut(s) 49, 2321
BsgI GTGCAG 2 cut(s) 206, 2954
BshFI GGCC 2 cut(s) 2109, 2283
BshNI GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BshVI ATCGAT 1 cut(s) 978
BsiHKAI GWGCWC 5 cut(s) 147, 2313, 2397, 2496, 2580
BsiHKCI CYCGRG 2 cut(s) 113, 1524
BsiSI CCGG 2 cut(s) 1073, 2076
BslFI GGGAC 3 cut(s) 413, 600, 937
BslI CCNNNNNNNGG 3 cut(s) 261, 545, 1815
BsmAI GTCTC 5 cut(s) 449, 672, 1552, 2232, 2417
BsmBI CGTCTC 2 cut(s) 449, 2232
BsmFI GGGAC 3 cut(s) 413, 600, 937
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 2 cut(s) 2109, 2283
BsoBI CYCGRG 2 cut(s) 113, 1524
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 6 cut(s) 81, 147, 2313, 2397, 2496, 2580
Bsp13I TCCGGA 1 cut(s) 1072
BspACI CCGC 5 cut(s) 30, 1113, 1124, 1851, 2331
BspANI GGCC 2 cut(s) 2109, 2283
BspCNI CTCAG 6 cut(s) 694, 1375, 1562, 2427, 2816, 3000
BspDI ATCGAT 1 cut(s) 978
BspEI TCCGGA 1 cut(s) 1072
BspLI GGNNCC 4 cut(s) 916, 1083, 1820, 1982
BspMAI CTGCAG 2 cut(s) 1881, 1890
BspMI ACCTGC 1 cut(s) 2976
BspPI GGATC 3 cut(s) 203, 1546, 1698
BspQI GCTCTTC 2 cut(s) 167, 2827
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BspTI CTTAAG 1 cut(s) 2915
BsrDI GCAATG 1 cut(s) 1643
BsrI ACTGG 5 cut(s) 197, 1044, 1710, 1981, 2354
BssECI CCNNGG 4 cut(s) 603, 2076, 2487, 2829
BssNI GRCGYC 2 cut(s) 90, 2700
Bst2UI CCWGG 5 cut(s) 605, 1383, 2489, 2831, 2998
Bst4CI ACNGT 8 cut(s) 786, 1301, 2043, 2117, 2249, 2472, 2507, 2975
Bst6I CTCTTC 8 cut(s) 167, 447, 1008, 2034, 2382, 2527, 2539, 2827
BstACI GRCGYC 2 cut(s) 90, 2700
BstAFI CTTAAG 1 cut(s) 2915
BstAPI GCANNNNNTGC 2 cut(s) 1859, 1885
BstBAI YACGTR 2 cut(s) 1748, 2983
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 5 cut(s) 884, 1128, 2201, 2423, 2902
BstF5I GGATG 8 cut(s) 157, 247, 367, 832, 859, 1069, 2016, 2714
BstH2I RGCGCY 1 cut(s) 838
BstHHI GCGC 1 cut(s) 837
BstMAI GTCTC 5 cut(s) 449, 672, 1552, 2232, 2417
BstMWI GCNNNNNNNGC 2 cut(s) 1859, 1885
BstNI CCWGG 5 cut(s) 605, 1383, 2489, 2831, 2998
BstNSI RCATGY 5 cut(s) 1270, 1420, 2002, 2156, 2622
BstSCI CCNGG 6 cut(s) 603, 1381, 2075, 2487, 2829, 2996
BstSFI CTRYAG 3 cut(s) 1877, 1886, 1912
BstSNI TACGTA 1 cut(s) 2983
BstV1I GCAGC 5 cut(s) 277, 2208, 2353, 2807, 2810
BstV2I GAAGAC 3 cut(s) 48, 602, 1667
BstX2I RGATCY 4 cut(s) 716, 1538, 1756, 2065
BstXI CCANNNNNNTGG 1 cut(s) 2321
BstYI RGATCY 4 cut(s) 716, 1538, 1756, 2065
Bsu15I ATCGAT 1 cut(s) 978
Bsu36I CCTNAGG 1 cut(s) 1314
BsuRI GGCC 2 cut(s) 2109, 2283
BsuTUI ATCGAT 1 cut(s) 978
BtsCI GGATG 8 cut(s) 157, 247, 367, 832, 859, 1069, 2016, 2714
BtsI GCAGTG 2 cut(s) 1564, 2629
BtsIMutI CAGTG 5 cut(s) 1564, 1974, 2254, 2629, 2971
BveI ACCTGC 1 cut(s) 2976
Cac8I GCNNGC 5 cut(s) 884, 1128, 2201, 2423, 2902
CaiI CAGNNNCTG 3 cut(s) 1541, 1885, 2968
CfoI GCGC 1 cut(s) 837
Cfr13I GGNCC 1 cut(s) 2232
ClaI ATCGAT 1 cut(s) 978
CseI GACGC 4 cut(s) 79, 466, 1900, 2708
Csp6I GTAC 7 cut(s) 24, 319, 1819, 2129, 2166, 2206, 2984
CspCI CAANNNNNGTGG 2 cut(s) 2370, 2405
CviQI GTAC 7 cut(s) 24, 319, 1819, 2129, 2166, 2206, 2984
DraI TTTAAA 1 cut(s) 1048
DraIII CACNNNGTG 2 cut(s) 542, 1980
Eam1104I CTCTTC 8 cut(s) 167, 447, 1008, 2034, 2382, 2527, 2539, 2827
EarI CTCTTC 8 cut(s) 167, 447, 1008, 2034, 2382, 2527, 2539, 2827
Ecl136II GAGCTC 2 cut(s) 2395, 2578
Eco105I TACGTA 1 cut(s) 2983
Eco147I AGGCCT 1 cut(s) 2283
Eco24I GRGCYC 3 cut(s) 81, 2397, 2580
Eco32I GATATC 1 cut(s) 976
Eco47I GGWCC 1 cut(s) 2232
Eco47III AGCGCT 1 cut(s) 836
Eco53kI GAGCTC 2 cut(s) 2395, 2578
Eco57I CTGAAG 7 cut(s) 379, 872, 1259, 1563, 1670, 2015, 2907
Eco81I CCTNAGG 1 cut(s) 1314
Eco88I CYCGRG 2 cut(s) 113, 1524
EcoICRI GAGCTC 2 cut(s) 2395, 2578
EcoRII CCWGG 5 cut(s) 603, 1381, 2487, 2829, 2996
EcoRV GATATC 1 cut(s) 976
EcoT22I ATGCAT 1 cut(s) 1503
EcoT38I GRGCYC 3 cut(s) 81, 2397, 2580
Esp3I CGTCTC 2 cut(s) 449, 2232
FalI AAGNNNNNCTT 8 cut(s) 377, 409, 2115, 2147, 2536, 2568, 2733, 2765
FaqI GGGAC 3 cut(s) 413, 600, 937
FauNDI CATATG 1 cut(s) 1497
FbaI TGATCA 3 cut(s) 1261, 2440, 2850
FblI GTMKAC 1 cut(s) 2157
Fnu4HI GCNGC 7 cut(s) 30, 266, 2197, 2332, 2367, 2796, 2799
FokI GGATG 8 cut(s) 164, 254, 374, 839, 866, 1056, 2023, 2701
FriOI GRGCYC 3 cut(s) 81, 2397, 2580
Fsp4HI GCNGC 7 cut(s) 30, 266, 2197, 2332, 2367, 2796, 2799
GlaI GCGC 1 cut(s) 836
GluI GCNGC 7 cut(s) 30, 266, 2197, 2332, 2367, 2796, 2799
GsaI CCCAGC 2 cut(s) 53, 2325
GsuI CTGGAG 1 cut(s) 2510
HaeII RGCGCY 1 cut(s) 838
HaeIII GGCC 2 cut(s) 2109, 2283
HapII CCGG 2 cut(s) 1073, 2076
HgaI GACGC 4 cut(s) 79, 466, 1900, 2708
HhaI GCGC 1 cut(s) 837
Hin1I GRCGYC 2 cut(s) 90, 2700
Hin6I GCGC 1 cut(s) 835
HinP1I GCGC 1 cut(s) 835
HincII GTYRAC 3 cut(s) 94, 1806, 2158
HindII GTYRAC 3 cut(s) 94, 1806, 2158
HindIII AAGCTT 1 cut(s) 2918
HpaII CCGG 2 cut(s) 1073, 2076
HphI GGTGA 5 cut(s) 749, 1367, 1424, 2549, 2671
Hpy166II GTNNAC 7 cut(s) 94, 871, 1806, 2090, 2158, 2338, 2698
Hpy8I GTNNAC 7 cut(s) 94, 871, 1806, 2090, 2158, 2338, 2698
HpyCH4III ACNGT 8 cut(s) 786, 1301, 2043, 2117, 2249, 2472, 2507, 2975
HpyCH4IV ACGT 2 cut(s) 1747, 2982
HpyF10VI GCNNNNNNNGC 2 cut(s) 1859, 1885
HpySE526I ACGT 2 cut(s) 1747, 2982
Hsp92I GRCGYC 2 cut(s) 90, 2700
HspAI GCGC 1 cut(s) 835
Kpn2I TCCGGA 1 cut(s) 1072
KpnI GGTACC 1 cut(s) 1822
Ksp22I TGATCA 3 cut(s) 1261, 2440, 2850
LguI GCTCTTC 2 cut(s) 167, 2827
LmnI GCTCC 4 cut(s) 1066, 2316, 2392, 2491
Lsp1109I GCAGC 5 cut(s) 277, 2208, 2353, 2807, 2810
LweI GCATC 7 cut(s) 142, 232, 762, 1078, 1459, 2408, 2434
MaeII ACGT 2 cut(s) 1747, 2982
MaeIII GTNAC 8 cut(s) 562, 937, 1212, 1367, 1412, 1712, 2507, 2635
MfeI CAATTG 3 cut(s) 696, 1326, 2400
MflI RGATCY 4 cut(s) 716, 1538, 1756, 2065
MhlI GDGCHC 6 cut(s) 81, 147, 2313, 2397, 2496, 2580
MlyI GAGTC 2 cut(s) 337, 1132
MmeI TCCRAC 6 cut(s) 219, 469, 1001, 1549, 2256, 2311
Mph1103I ATGCAT 1 cut(s) 1503
MroI TCCGGA 1 cut(s) 1072
MroXI GAANNNNTTC 2 cut(s) 12, 1587
MslI CAYNNNNRTG 2 cut(s) 1500, 2723
MspA1I CMGCKG 2 cut(s) 482, 892
MspCI CTTAAG 1 cut(s) 2915
MspI CCGG 2 cut(s) 1073, 2076
MspR9I CCNGG 6 cut(s) 605, 1383, 2077, 2489, 2831, 2998
MunI CAATTG 3 cut(s) 696, 1326, 2400
Mva1269I GAATGC 1 cut(s) 460
MvaI CCWGG 5 cut(s) 605, 1383, 2489, 2831, 2998
MwoI GCNNNNNNNGC 2 cut(s) 1859, 1885
NciI CCSGG 1 cut(s) 2077
NdeI CATATG 1 cut(s) 1497
NlaIV GGNNCC 4 cut(s) 916, 1083, 1820, 1982
NmuCI GTSAC 4 cut(s) 937, 1412, 1712, 2635
NsiI ATGCAT 1 cut(s) 1503
NspI RCATGY 5 cut(s) 1270, 1420, 2002, 2156, 2622
NspV TTCGAA 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 113
PceI AGGCCT 1 cut(s) 2283
PciI ACATGT 2 cut(s) 1416, 2152
PciSI GCTCTTC 2 cut(s) 167, 2827
PctI GAATGC 1 cut(s) 460
PdmI GAANNNNTTC 2 cut(s) 12, 1587
PkrI GCNGC 7 cut(s) 31, 267, 2198, 2333, 2368, 2797, 2800
PleI GAGTC 2 cut(s) 336, 1132
PpsI GAGTC 2 cut(s) 336, 1132
Ppu21I YACGTR 2 cut(s) 1748, 2983
PscI ACATGT 2 cut(s) 1416, 2152
Psp124BI GAGCTC 2 cut(s) 2397, 2580
Psp6I CCWGG 5 cut(s) 603, 1381, 2487, 2829, 2996
PspFI CCCAGC 2 cut(s) 49, 2321
PspGI CCWGG 5 cut(s) 603, 1381, 2487, 2829, 2996
PspN4I GGNNCC 4 cut(s) 916, 1083, 1820, 1982
PspPI GGNCC 1 cut(s) 2232
PstI CTGCAG 2 cut(s) 1881, 1890
PstNI CAGNNNCTG 3 cut(s) 1541, 1885, 2968
PsuI RGATCY 4 cut(s) 716, 1538, 1756, 2065
PvuII CAGCTG 2 cut(s) 482, 892
RsaI GTAC 7 cut(s) 25, 320, 1820, 2130, 2167, 2207, 2985
RsaNI GTAC 7 cut(s) 24, 319, 1819, 2129, 2166, 2206, 2984
RseI CAYNNNNRTG 2 cut(s) 1500, 2723
SacI GAGCTC 2 cut(s) 2397, 2580
SalI GTCGAC 1 cut(s) 2156
SapI GCTCTTC 2 cut(s) 167, 2827
SatI GCNGC 7 cut(s) 30, 266, 2197, 2332, 2367, 2796, 2799
Sau96I GGNCC 1 cut(s) 2232
SchI GAGTC 2 cut(s) 337, 1132
ScrFI CCNGG 6 cut(s) 605, 1383, 2077, 2489, 2831, 2998
SduI GDGCHC 6 cut(s) 81, 147, 2313, 2397, 2496, 2580
SfaNI GCATC 7 cut(s) 142, 232, 762, 1078, 1459, 2408, 2434
SfcI CTRYAG 3 cut(s) 1877, 1886, 1912
Sfr274I CTCGAG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 249
SinI GGWCC 1 cut(s) 2232
SlaI CTCGAG 1 cut(s) 113
SmiMI CAYNNNNRTG 2 cut(s) 1500, 2723
SmlI CTYRAG 8 cut(s) 113, 269, 712, 1247, 1735, 1967, 2456, 2915
SmoI CTYRAG 8 cut(s) 113, 269, 712, 1247, 1735, 1967, 2456, 2915
SnaBI TACGTA 1 cut(s) 2983
SpeI ACTAGT 1 cut(s) 2734
SseBI AGGCCT 1 cut(s) 2283
SsiI CCGC 5 cut(s) 30, 1113, 1124, 1851, 2331
SstI GAGCTC 2 cut(s) 2397, 2580
StuI AGGCCT 1 cut(s) 2283
StyD4I CCNGG 6 cut(s) 603, 1381, 2075, 2487, 2829, 2996
TaaI ACNGT 8 cut(s) 786, 1301, 2043, 2117, 2249, 2472, 2507, 2975
TaiI ACGT 2 cut(s) 1750, 2985
TaqI TCGA 8 cut(s) 114, 249, 331, 978, 1176, 2157, 2656, 2765
TatI WGTACW 1 cut(s) 318
TauI GCSGC 2 cut(s) 32, 2334
TscAI CASTG 5 cut(s) 1564, 1981, 2254, 2629, 2978
TseFI GTSAC 4 cut(s) 937, 1412, 1712, 2635
TseI GCWGC 5 cut(s) 265, 2196, 2366, 2795, 2798
Tsp45I GTSAC 4 cut(s) 937, 1412, 1712, 2635
TspGWI ACGGA 1 cut(s) 1783
TspRI CASTG 5 cut(s) 1564, 1981, 2254, 2629, 2978
Vha464I CTTAAG 1 cut(s) 2915
VpaK11BI GGWCC 1 cut(s) 2232
XapI RAATTY 8 cut(s) 71, 650, 1479, 1704, 1789, 2188, 2299, 2843
XceI RCATGY 5 cut(s) 1270, 1420, 2002, 2156, 2622
XhoI CTCGAG 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 2157
XmnI GAANNNNTTC 2 cut(s) 12, 1587
Zsp2I ATGCAT 1 cut(s) 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.