RchiOBHm_Chr4g0420141

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000725
Physical Location & Seq
Forward (+)
45808372 .. 45811481
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38991

Sequence Viewer

Length: 3000 bp
ATGGTTGAAGCAGTTCCATTTGGCACTGCAGCTGACATACTCATCAAGCTCGGGTCTTCCACTTTCCGGGAGCTCGGGCTCGTCTACGGCGTCAACAAGGAAATGAAGAAGCTTGAGAACACTCTCTCCACCATCAAAGCTGTTCTCTTAGACGCAGAGGAGAAGCAAGAGAAGAGCCATCTGGTGCAGGACTGGATCAGGAAGCTCAGCGAGGTTGTGTACGAGGCCGATGATTTGTTGGATGCATTCGAAACCAAAGCTAGGCAGCATGAGAATCATCAGGTGCGTGAGTTCTTTTCAAAGACACTTGCTTTTAGATACAAGATGGGTCGAAAACTGAGAGAAACTAGAGAGAGATTGGATGAGATTGCAGGGGATATTTCAAAGTTCAACTTCAGGGATAGGTCTTTGACAATCTCAGATATTGGGATTGAGATGAATAATAAGAGAGAAGAGACGCATTCGTTTATGGTGGAGTCGGCTGTAATTGGAAGAAAGAGAGAGAAGGAGGAAATGGTTAAGTTTTTGATATTCCCACCAAGTACTCAAGAAAATGTGTCTGTGACTGCCATTGTTGGTATTGGGGGACTTGGGAAGACCACCCTTGCTAAAATGTTGTATAATGATGATAGGGTAGTGAACTATTTTGAGATTAAGATGTGGGTGTGGGCGTATGGAAGTTTTGATTTATATTCAGTTGCGGAGAAAATTTTGAGATCTATTGCTACGAATGAACTAGAAGATTCGGAGTTTTATTACCAGCGTATTGTGCTGCAGAAACAGTTTCTTATGGAGGAGTTGCAGATTCTGCTTCGGAAGAAATTGGATGGGAAGCGCTACCTCCTTGTTCTGGATGATGTGTGGAATGTGAAGCGCGAAAGTTGGCTTCAGCTGAATGATTTGTTGAAAGGTGGTGCCAAGGGGAGTAAGATTGTTGTGACAACTCGTAGCACGAAAGTTACCGCGCTTATGGATGTTGATTCCCCTTTTGTGTTGCAAGATCTATCTGAGGAAGAGAGTTGGACATTGTTTAAGCAACTGGCTTTTAAAGATGGAAGGGAGGAGGAGCATCCGGAGTTGGTGCCAATAGGAAGAAGAATTGTGAAGAAATGCCGAGGTGTTCCTCTTGCAATAAGGACTCTAGGAAGCATGATGAGATACAAACCTAATGAAAGAGAGTGGATTTCGATTCAAAATACAGAGATCTGGGAGTTACCAAAAGAGGAGAATGATGTTTTGAAGATACTGAAGTTAAGCTATGATCACATGCCAATTTATTTGAAACAATGCTTCTCCTACTGCGCATTGTTCCCCAAGGGAAATGAAGTTGAGAAAAAAATGTTGGTCGAACTTTGGATAGCTCAGGGTTACATTCACCTTCCTGGTAAAGAGAGGAACTTAGAAGAAATTGGTGACCTGTATTTCAAGGAATTGATAGAGAGATCATTATTTCAAGAGGTCAAGAAAGATGCCAATGGAAATGTAATTAGTTGTCATATGCATGATCTTATACACGATCTTTCACGGGCAGTTGCAGGATCTGAATGCTCAATTTGCAGTGTTGATGTTGGAGATATATCTGAAAGAGTTCATCATGTTTCATTTAGTTGTTATCTGCCTTCTTCATGGGAAGTTCCGATAGCGTTGCTCAAGGCAAAGAAGACTAGGACATTTATTCTACCACTTCAGGATCAAGCATATAGAATTTCCAGTCACAATACAATCATTTCAAACTTGAGGCGCTTACGTGTGTTAGATCTGCATAACCTAAGAATTGATGAGCTTTCAAATTCCATAGGCAAGTTGACCCATCTTAGGTACCTTGACCTCTCTGGTAATGTTAGTATGACAGCACTCCCTGCTTCTATAAGCAATCTGCAGAATCTACAGACGCTTAAACTCAACAGGTGCTACAGACTTGAACAACTACCTAGAGATATTGGAAAGATGATCAGCCTTAGACATCTTGAGATCACTGGGTGCCGCAAATTGAATCACATGCCACCTGGGCTGGGAAAGTTGGTATCTCTTCAGACATTACCGTTATTCATCTTGGGCAGGAAAGCTCTCTTTTCCGGTGTGAGTGGTGGAATAGATGAGTTGAGTGGCTTGAACTGTCTTAAAGGAGTGCTTCATGTTGCCCATCTTGAACATGTTGACAATGTCACAATAGATTCAAGGAGTTCAAATTTGAGCAGCAAGCAATATCTTGAATCCTTAAAACTAATTTGGTCCAGAGATGACGACAGTGAAAGTAATGAAGATCACATGGTGTTGGAAGGCCTTCAGCCCCATCAAAATTTGAAAGTACTGCATATAGCTGGGTATTGCGGTGTTCAATTCCCCAACTGGCTGATGAACAACGCTGCATCGTCTCTTCCAAGTCTAGTTGAGCTCACCATTGAGGGCTGTCTCAGATGCCAGCATCTTCCACCACTGGATCAACTGCCTTCTCTTAAGTTTCTCAAACTATATTCTCTAAGTTGCCTCGAGCACATTAACAACAGTTACTCATCTCCCTCTTGTAGTTTGGTTGGGGGAGGAAGAGAGCCTTTGTTTTTTCCATCATTGAAAGAGCTCACACTTTATGACTTGCCACTCTTGAAGGAGTGGCAAACCTGCAGTGATCAAGATGGGACAACAACAATGCCAAAGCAGCAGCAGCTTTCATTTCCTTCCCTCACCAAATTGACTATCGTGGACGCCCCTAACTTAATGTCCATGCCGATGCTTCCACTAGTTGAAGAATTGCTTATGGACACCGTCTCTGAGAAGTTGTTGCAGTCCATGTTGATGACAGCAGCCTCAGAAAGCCAAACAAGCTCTTCCTCCCTTGCTAGATTGAAATTTCTGATCATCAAATCATGCAATGATCTGGTCTCTTTCCCTGAAGATAGGATAAATAACCTCACTTCTCTTGAGCTCCTAAAGATTATAGATTGTGGAAACTTCACATCCCTACCACATAATCTGCACTGTGAATACGTACTTAGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

999

Amino Acids

113.86

Weight (kDa)

6.35

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 15 - 97 4.2e-22 Rx N-terminal domain
NB-ARC PF00931 182 - 350 1.2e-30 NB-ARC domain
WHD_DRP PF23559 436 - 507 6.5e-25 Disease resistance protein Winged helix domain
LRR_14 PF23598 575 - 893 3.2e-33 Leucine-rich repeat region
LRR_8 PF13855 580 - 637 5.1e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 698 - 826 5.2e-35 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1303
Acc36I ACCTGC 1 cut(s) 2627
Acc65I GGTACC 1 cut(s) 1818
AccB1I GGYRCC 4 cut(s) 914, 1081, 1818, 1980
AccI GTMKAC 1 cut(s) 84
AccII CGCG 2 cut(s) 876, 965
AccIII TCCGGA 1 cut(s) 1072
AciI CCGC 4 cut(s) 701, 963, 1984, 2331
AclWI GGATC 4 cut(s) 203, 1546, 1698, 2448
AcsI RAATTY 6 cut(s) 708, 1704, 1789, 2188, 2299, 2846
AcuI CTGAAG 7 cut(s) 379, 872, 1268, 1670, 2015, 2270, 2910
AcyI GRCGYC 2 cut(s) 90, 2703
AdeI CACNNNGTG 2 cut(s) 1980, 2272
AfaI GTAC 5 cut(s) 221, 544, 1820, 2310, 2988
AfeI AGCGCT 1 cut(s) 836
AfiI CCNNNNNNNGG 5 cut(s) 66, 261, 850, 1815, 2012
AflII CTTAAG 1 cut(s) 2456
AflIII ACRYGT 2 cut(s) 1747, 2152
AhlI ACTAGT 1 cut(s) 2737
AjnI CCWGG 2 cut(s) 1381, 2005
AjuI GAANNNNNNNTTGG 2 cut(s) 1325, 1357
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
Alw21I GWGCWC 5 cut(s) 75, 2397, 2496, 2580, 2925
Alw26I GTCTC 5 cut(s) 449, 2379, 2417, 2770, 2884
AlwI GGATC 4 cut(s) 203, 1546, 1698, 2448
AlwNI CAGNNNCTG 2 cut(s) 808, 1541
Ama87I CYCGRG 3 cut(s) 50, 74, 2489
Aor13HI TCCGGA 1 cut(s) 1072
Aor51HI AGCGCT 1 cut(s) 836
AoxI GGCC 2 cut(s) 225, 2281
ApeKI GCWGC 9 cut(s) 29, 265, 772, 2196, 2366, 2656, 2659, 2662, 2801
ApoI RAATTY 6 cut(s) 708, 1704, 1789, 2188, 2299, 2846
Asp700I GAANNNNTTC 3 cut(s) 12, 1587, 2283
Asp718I GGTACC 1 cut(s) 1818
AspLEI GCGC 5 cut(s) 837, 876, 967, 1304, 1743
AspS9I GGNCC 1 cut(s) 2232
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 4 cut(s) 1367, 1424, 2389, 2674
AsuII TTCGAA 1 cut(s) 249
AvaI CYCGRG 3 cut(s) 50, 74, 2489
AvaII GGWCC 1 cut(s) 2232
BaeI ACNNNNGTAYC 2 cut(s) 1235, 1268
BanI GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BanII GRGCYC 5 cut(s) 75, 81, 2397, 2580, 2925
BbsI GAAGAC 3 cut(s) 48, 602, 1667
Bbv12I GWGCWC 5 cut(s) 75, 2397, 2496, 2580, 2925
BbvI GCAGC 9 cut(s) 41, 277, 759, 2208, 2353, 2668, 2671, 2674, 2813
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 1627, 1661
BciT130I CCWGG 2 cut(s) 1383, 2007
BclI TGATCA 4 cut(s) 1261, 1950, 2626, 2853
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 5 cut(s) 449, 2379, 2417, 2770, 2884
BcuI ACTAGT 1 cut(s) 2737
BfaI CTAG 9 cut(s) 261, 348, 737, 1142, 1665, 1932, 2387, 2738, 2838
BfmI CTRYAG 6 cut(s) 27, 773, 1877, 1886, 1912, 2620
BfoI RGCGCY 2 cut(s) 838, 1744
BfrI CTTAAG 1 cut(s) 2456
BfuAI ACCTGC 1 cut(s) 2627
BglI GCCNNNNNGGC 1 cut(s) 2008
BglII AGATCT 4 cut(s) 716, 1000, 1203, 1756
BlpI GCTNAGC 1 cut(s) 206
BmcAI AGTACT 2 cut(s) 544, 2310
Bme1390I CCNGG 3 cut(s) 68, 1383, 2007
Bme18I GGWCC 1 cut(s) 2232
BmeT110I CYCGRG 3 cut(s) 50, 74, 2489
BmgT120I GGNCC 1 cut(s) 2232
BmiI GGNNCC 4 cut(s) 916, 1083, 1820, 1982
BmrFI CCNGG 3 cut(s) 68, 1383, 2007
BmrI ACTGGG 1 cut(s) 1986
BmsI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2718
BmuI ACTGGG 1 cut(s) 1986
BpiI GAAGAC 3 cut(s) 48, 602, 1667
Bpu10I CCTNAGC 1 cut(s) 1362
Bpu1102I GCTNAGC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 249
BpuEI CTTGAG 6 cut(s) 134, 531, 1634, 1756, 1988, 2939
BpuMI CCSGG 1 cut(s) 68
BsaAI YACGTR 2 cut(s) 1748, 2986
BsaHI GRCGYC 2 cut(s) 90, 2703
BsaI GGTCTC 1 cut(s) 2884
BsaJI CCNNGG 4 cut(s) 918, 1114, 1314, 2006
BsaWI WCCGGW 2 cut(s) 1072, 2075
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 261, 850, 1815, 2012
Bse1I ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
Bse3DI GCAATG 1 cut(s) 2875
BseAI TCCGGA 1 cut(s) 1072
BseBI CCWGG 2 cut(s) 1383, 2007
BseDI CCNNGG 4 cut(s) 918, 1114, 1314, 2006
BseGI GGATG 7 cut(s) 247, 367, 832, 859, 979, 1069, 2954
BseLI CCNNNNNNNGG 5 cut(s) 66, 261, 850, 1815, 2012
BseMI GCAATG 1 cut(s) 2875
BseMII CTCAG 8 cut(s) 220, 329, 432, 999, 1376, 2428, 2760, 2820
BseNI ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
BseRI GAGGAG 5 cut(s) 173, 809, 1076, 1079, 1238
BseXI GCAGC 9 cut(s) 41, 277, 759, 2208, 2353, 2668, 2671, 2674, 2813
BseYI CCCAGC 2 cut(s) 2011, 2321
BsgI GTGCAG 2 cut(s) 206, 2957
Bsh1236I CGCG 2 cut(s) 876, 965
BshFI GGCC 2 cut(s) 227, 2283
BshNI GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BsiHKAI GWGCWC 5 cut(s) 75, 2397, 2496, 2580, 2925
BsiHKCI CYCGRG 3 cut(s) 50, 74, 2489
BsiSI CCGG 3 cut(s) 67, 1073, 2076
BslFI GGGAC 2 cut(s) 600, 2650
BslI CCNNNNNNNGG 5 cut(s) 66, 261, 850, 1815, 2012
BsmAI GTCTC 5 cut(s) 449, 2379, 2417, 2770, 2884
BsmBI CGTCTC 3 cut(s) 449, 2379, 2770
BsmFI GGGAC 2 cut(s) 600, 2650
BsmI GAATGC 3 cut(s) 245, 460, 1550
BsnI GGCC 2 cut(s) 227, 2283
Bso31I GGTCTC 1 cut(s) 2884
BsoBI CYCGRG 3 cut(s) 50, 74, 2489
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 6 cut(s) 75, 81, 2397, 2496, 2580, 2925
Bsp13I TCCGGA 1 cut(s) 1072
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 4 cut(s) 701, 963, 1984, 2331
BspANI GGCC 2 cut(s) 227, 2283
BspCNI CTCAG 8 cut(s) 219, 330, 431, 1000, 1375, 2427, 2761, 2819
BspEI TCCGGA 1 cut(s) 1072
BspFNI CGCG 2 cut(s) 876, 965
BspLI GGNNCC 4 cut(s) 916, 1083, 1820, 1982
BspMAI CTGCAG 4 cut(s) 31, 777, 1881, 2624
BspMI ACCTGC 1 cut(s) 2627
BspPI GGATC 4 cut(s) 203, 1546, 1698, 2448
BspQI GCTCTTC 2 cut(s) 167, 2830
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BspTI CTTAAG 1 cut(s) 2456
BspTNI GGTCTC 1 cut(s) 2884
BsrDI GCAATG 1 cut(s) 2875
BsrI ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
BssECI CCNNGG 4 cut(s) 918, 1114, 1314, 2006
BssNI GRCGYC 2 cut(s) 90, 2703
BssT1I CCWWGG 2 cut(s) 918, 1314
Bst2UI CCWGG 2 cut(s) 1383, 2007
Bst4CI ACNGT 7 cut(s) 783, 2043, 2117, 2249, 2507, 2764, 2978
Bst6I CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2539, 2830
BstACI GRCGYC 2 cut(s) 90, 2703
BstAFI CTTAAG 1 cut(s) 2456
BstAPI GCANNNNNTGC 2 cut(s) 808, 1859
BstBAI YACGTR 2 cut(s) 1748, 2986
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 2201, 2423
BstEII GGTNACC 1 cut(s) 1412
BstF5I GGATG 7 cut(s) 247, 367, 832, 859, 979, 1069, 2954
BstFNI CGCG 2 cut(s) 876, 965
BstH2I RGCGCY 2 cut(s) 838, 1744
BstHHI GCGC 5 cut(s) 837, 876, 967, 1304, 1743
BstMAI GTCTC 5 cut(s) 449, 2379, 2417, 2770, 2884
BstMWI GCNNNNNNNGC 8 cut(s) 769, 808, 1554, 1859, 2008, 2656, 2662, 2820
BstNI CCWGG 2 cut(s) 1383, 2007
BstNSI RCATGY 3 cut(s) 1270, 2002, 2156
BstPI GGTNACC 1 cut(s) 1412
BstSCI CCNGG 3 cut(s) 66, 1381, 2005
BstSFI CTRYAG 6 cut(s) 27, 773, 1877, 1886, 1912, 2620
BstSNI TACGTA 1 cut(s) 2986
BstUI CGCG 2 cut(s) 876, 965
BstV1I GCAGC 9 cut(s) 41, 277, 759, 2208, 2353, 2668, 2671, 2674, 2813
BstV2I GAAGAC 3 cut(s) 48, 602, 1667
BstX2I RGATCY 5 cut(s) 716, 1000, 1203, 1538, 1756
BstYI RGATCY 5 cut(s) 716, 1000, 1203, 1538, 1756
BsuRI GGCC 2 cut(s) 227, 2283
BtsCI GGATG 7 cut(s) 247, 367, 832, 859, 979, 1069, 2954
BtsI GCAGTG 3 cut(s) 24, 1564, 2629
BtsIMutI CAGTG 7 cut(s) 24, 1564, 1974, 2254, 2435, 2629, 2974
BveI ACCTGC 1 cut(s) 2627
Cac8I GCNNGC 2 cut(s) 2201, 2423
CaiI CAGNNNCTG 2 cut(s) 808, 1541
CfoI GCGC 5 cut(s) 837, 876, 967, 1304, 1743
Cfr13I GGNCC 1 cut(s) 2232
CseI GACGC 5 cut(s) 79, 161, 466, 1900, 2711
Csp6I GTAC 5 cut(s) 220, 543, 1819, 2309, 2987
CviQI GTAC 5 cut(s) 220, 543, 1819, 2309, 2987
DraI TTTAAA 1 cut(s) 1048
DraIII CACNNNGTG 2 cut(s) 1980, 2272
Eam1104I CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2539, 2830
EarI CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2539, 2830
Ecl136II GAGCTC 4 cut(s) 73, 2395, 2578, 2923
Eco105I TACGTA 1 cut(s) 2986
Eco130I CCWWGG 2 cut(s) 918, 1314
Eco147I AGGCCT 1 cut(s) 2283
Eco24I GRGCYC 5 cut(s) 75, 81, 2397, 2580, 2925
Eco31I GGTCTC 1 cut(s) 2884
Eco47I GGWCC 1 cut(s) 2232
Eco47III AGCGCT 1 cut(s) 836
Eco53kI GAGCTC 4 cut(s) 73, 2395, 2578, 2923
Eco57I CTGAAG 7 cut(s) 379, 872, 1268, 1670, 2015, 2270, 2910
Eco88I CYCGRG 3 cut(s) 50, 74, 2489
Eco91I GGTNACC 1 cut(s) 1412
EcoICRI GAGCTC 4 cut(s) 73, 2395, 2578, 2923
EcoO65I GGTNACC 1 cut(s) 1412
EcoRII CCWGG 2 cut(s) 1381, 2005
EcoT14I CCWWGG 2 cut(s) 918, 1314
EcoT22I ATGCAT 2 cut(s) 247, 1503
EcoT38I GRGCYC 5 cut(s) 75, 81, 2397, 2580, 2925
ErhI CCWWGG 2 cut(s) 918, 1314
Esp3I CGTCTC 3 cut(s) 449, 2379, 2770
FalI AAGNNNNNCTT 8 cut(s) 377, 409, 2115, 2147, 2536, 2568, 2736, 2768
FaqI GGGAC 2 cut(s) 600, 2650
FauNDI CATATG 1 cut(s) 1497
FbaI TGATCA 4 cut(s) 1261, 1950, 2626, 2853
FblI GTMKAC 1 cut(s) 84
FokI GGATG 7 cut(s) 254, 374, 839, 866, 986, 1056, 2941
FriOI GRGCYC 5 cut(s) 75, 81, 2397, 2580, 2925
FspBI CTAG 9 cut(s) 261, 348, 737, 1142, 1665, 1932, 2387, 2738, 2838
FspI TGCGCA 1 cut(s) 1303
GlaI GCGC 5 cut(s) 836, 875, 966, 1303, 1742
GsaI CCCAGC 2 cut(s) 2015, 2325
HaeII RGCGCY 2 cut(s) 838, 1744
HaeIII GGCC 2 cut(s) 227, 2283
HapII CCGG 3 cut(s) 67, 1073, 2076
HgaI GACGC 5 cut(s) 79, 161, 466, 1900, 2711
HhaI GCGC 5 cut(s) 837, 876, 967, 1304, 1743
Hin1I GRCGYC 2 cut(s) 90, 2703
Hin6I GCGC 5 cut(s) 835, 874, 965, 1302, 1741
HinP1I GCGC 5 cut(s) 835, 874, 965, 1302, 1741
HincII GTYRAC 3 cut(s) 94, 1806, 2158
HindII GTYRAC 3 cut(s) 94, 1806, 2158
HindIII AAGCTT 1 cut(s) 110
HpaII CCGG 3 cut(s) 67, 1073, 2076
HphI GGTGA 4 cut(s) 1367, 1424, 2389, 2674
Hpy166II GTNNAC 7 cut(s) 85, 94, 220, 640, 1806, 2158, 2701
Hpy8I GTNNAC 7 cut(s) 85, 94, 220, 640, 1806, 2158, 2701
HpyAV CCTTC 9 cut(s) 499, 1050, 1388, 1629, 2273, 2294, 2460, 2599, 2685
HpyCH4III ACNGT 7 cut(s) 783, 2043, 2117, 2249, 2507, 2764, 2978
HpyCH4IV ACGT 2 cut(s) 1747, 2985
HpyF10VI GCNNNNNNNGC 8 cut(s) 769, 808, 1554, 1859, 2008, 2656, 2662, 2820
HpySE526I ACGT 2 cut(s) 1747, 2985
Hsp92I GRCGYC 2 cut(s) 90, 2703
HspAI GCGC 5 cut(s) 835, 874, 965, 1302, 1741
Kpn2I TCCGGA 1 cut(s) 1072
KpnI GGTACC 1 cut(s) 1822
Ksp22I TGATCA 4 cut(s) 1261, 1950, 2626, 2853
LguI GCTCTTC 2 cut(s) 167, 2830
LmnI GCTCC 3 cut(s) 70, 1066, 2928
Lsp1109I GCAGC 9 cut(s) 41, 277, 759, 2208, 2353, 2668, 2671, 2674, 2813
LweI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2718
MaeI CTAG 9 cut(s) 261, 348, 737, 1142, 1665, 1932, 2387, 2738, 2838
MaeII ACGT 2 cut(s) 1747, 2985
MaeIII GTNAC 9 cut(s) 562, 937, 958, 1212, 1367, 1412, 1712, 2164, 2507
MflI RGATCY 5 cut(s) 716, 1000, 1203, 1538, 1756
MhlI GDGCHC 6 cut(s) 75, 81, 2397, 2496, 2580, 2925
MlyI GAGTC 2 cut(s) 485, 1132
MmeI TCCRAC 4 cut(s) 219, 1001, 1549, 2256
Mph1103I ATGCAT 2 cut(s) 247, 1503
MroI TCCGGA 1 cut(s) 1072
MroXI GAANNNNTTC 3 cut(s) 12, 1587, 2283
MslI CAYNNNNRTG 3 cut(s) 1500, 2726, 2792
MspA1I CMGCKG 2 cut(s) 32, 892
MspCI CTTAAG 1 cut(s) 2456
MspI CCGG 3 cut(s) 67, 1073, 2076
MspR9I CCNGG 3 cut(s) 68, 1383, 2007
Mva1269I GAATGC 3 cut(s) 245, 460, 1550
MvaI CCWGG 2 cut(s) 1383, 2007
MvnI CGCG 2 cut(s) 876, 965
MwoI GCNNNNNNNGC 8 cut(s) 769, 808, 1554, 1859, 2008, 2656, 2662, 2820
NciI CCSGG 1 cut(s) 68
NdeI CATATG 1 cut(s) 1497
NlaIV GGNNCC 4 cut(s) 916, 1083, 1820, 1982
NmeAIII GCCGAG 1 cut(s) 1139
NmuCI GTSAC 5 cut(s) 562, 937, 1412, 1712, 2164
NsbI TGCGCA 1 cut(s) 1303
NsiI ATGCAT 2 cut(s) 247, 1503
NspI RCATGY 3 cut(s) 1270, 2002, 2156
NspV TTCGAA 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 2489
PceI AGGCCT 1 cut(s) 2283
PciI ACATGT 1 cut(s) 2152
PciSI GCTCTTC 2 cut(s) 167, 2830
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 3 cut(s) 245, 460, 1550
PdmI GAANNNNTTC 3 cut(s) 12, 1587, 2283
PfeI GAWTC 9 cut(s) 274, 743, 805, 980, 1189, 1882, 1993, 2174, 2213
PflFI GACNNNGTC 2 cut(s) 2162, 2762
PfoI TCCNGGA 1 cut(s) 66
PleI GAGTC 2 cut(s) 484, 1132
PpsI GAGTC 2 cut(s) 484, 1132
Ppu21I YACGTR 2 cut(s) 1748, 2986
PscI ACATGT 1 cut(s) 2152
Psp124BI GAGCTC 4 cut(s) 75, 2397, 2580, 2925
Psp6I CCWGG 2 cut(s) 1381, 2005
PspEI GGTNACC 1 cut(s) 1412
PspFI CCCAGC 2 cut(s) 2011, 2321
PspGI CCWGG 2 cut(s) 1381, 2005
PspN4I GGNNCC 4 cut(s) 916, 1083, 1820, 1982
PspPI GGNCC 1 cut(s) 2232
PspXI VCTCGAGB 1 cut(s) 2489
PstI CTGCAG 4 cut(s) 31, 777, 1881, 2624
PstNI CAGNNNCTG 2 cut(s) 808, 1541
PsuI RGATCY 5 cut(s) 716, 1000, 1203, 1538, 1756
PsyI GACNNNGTC 2 cut(s) 2162, 2762
PvuII CAGCTG 2 cut(s) 32, 892
RsaI GTAC 5 cut(s) 221, 544, 1820, 2310, 2988
RsaNI GTAC 5 cut(s) 220, 543, 1819, 2309, 2987
RseI CAYNNNNRTG 3 cut(s) 1500, 2726, 2792
SacI GAGCTC 4 cut(s) 75, 2397, 2580, 2925
SapI GCTCTTC 2 cut(s) 167, 2830
Sau96I GGNCC 1 cut(s) 2232
ScaI AGTACT 2 cut(s) 544, 2310
SchI GAGTC 2 cut(s) 485, 1132
ScrFI CCNGG 3 cut(s) 68, 1383, 2007
SduI GDGCHC 6 cut(s) 75, 81, 2397, 2496, 2580, 2925
SfaNI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2718
SfcI CTRYAG 6 cut(s) 27, 773, 1877, 1886, 1912, 2620
Sfr274I CTCGAG 1 cut(s) 2489
SfuI TTCGAA 1 cut(s) 249
SinI GGWCC 1 cut(s) 2232
SlaI CTCGAG 1 cut(s) 2489
SmiMI CAYNNNNRTG 3 cut(s) 1500, 2726, 2792
SmlI CTYRAG 8 cut(s) 113, 546, 1649, 1735, 1967, 2456, 2489, 2918
SmoI CTYRAG 8 cut(s) 113, 546, 1649, 1735, 1967, 2456, 2489, 2918
SnaBI TACGTA 1 cut(s) 2986
SpeI ACTAGT 1 cut(s) 2737
SseBI AGGCCT 1 cut(s) 2283
SsiI CCGC 4 cut(s) 701, 963, 1984, 2331
SspMI CTAG 9 cut(s) 261, 348, 737, 1142, 1665, 1932, 2387, 2738, 2838
SstI GAGCTC 4 cut(s) 75, 2397, 2580, 2925
StuI AGGCCT 1 cut(s) 2283
StyD4I CCNGG 3 cut(s) 66, 1381, 2005
StyI CCWWGG 2 cut(s) 918, 1314
TaaI ACNGT 7 cut(s) 783, 2043, 2117, 2249, 2507, 2764, 2978
TaiI ACGT 2 cut(s) 1750, 2988
TaqI TCGA 5 cut(s) 249, 331, 1187, 1347, 2490
TatI WGTACW 2 cut(s) 542, 2308
TauI GCSGC 1 cut(s) 1986
TfiI GAWTC 9 cut(s) 274, 743, 805, 980, 1189, 1882, 1993, 2174, 2213
TscAI CASTG 7 cut(s) 31, 1564, 1981, 2254, 2442, 2629, 2981
TseFI GTSAC 5 cut(s) 562, 937, 1412, 1712, 2164
TseI GCWGC 9 cut(s) 29, 265, 772, 2196, 2366, 2656, 2659, 2662, 2801
Tsp45I GTSAC 5 cut(s) 562, 937, 1412, 1712, 2164
TspRI CASTG 7 cut(s) 31, 1564, 1981, 2254, 2442, 2629, 2981
Tth111I GACNNNGTC 2 cut(s) 2162, 2762
Vha464I CTTAAG 1 cut(s) 2456
VpaK11BI GGWCC 1 cut(s) 2232
XapI RAATTY 6 cut(s) 708, 1704, 1789, 2188, 2299, 2846
XceI RCATGY 3 cut(s) 1270, 2002, 2156
XhoI CTCGAG 1 cut(s) 2489
XmiI GTMKAC 1 cut(s) 84
XmnI GAANNNNTTC 3 cut(s) 12, 1587, 2283
XspI CTAG 9 cut(s) 261, 348, 737, 1142, 1665, 1932, 2387, 2738, 2838
ZrmI AGTACT 2 cut(s) 544, 2310
Zsp2I ATGCAT 2 cut(s) 247, 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.