MD07G1024400.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000725
Physical Location & Seq
Reverse (-)
2041985 .. 2043237
1253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1024400.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCGGAAGGAGTTCTCTTCAACGTTGCAGGAGGGATCATTGAGAGGCTAGGCTCCCTAGCTTTCCAAGAGATCGGATTGATTTGGGGTGTCCAAGATGAGTTCCAGAAGCTCAAGGAGACAGTTGCTGGATTCCAAGCTATTCTTCTTGACGCTGAGCAAAAGCAAGCCAACAATGAAGTCAAACTGTGGCTCCAAAGCGTAGAAGACGCAATTTATGAAGCCGATGACGTGTTGGATGAGTTTAATACTAAAAATCAACAGAGAAAAATGGTGCCTGAAAATACAAAGCTGTCAAAAAAGGTGCGCCTTTTCTTTTCTAGCTCAAATCAACTTGTTTTTGGGCTAAAGATGGGTCATAAGATAAAAGATATTAACAGGAGGCTTAGTGAGGTTGCATCTCGTAGAATCTTTCACTTAGAAGTAAATCATGAAGATACACGATTTGTAAAGAGAGAGAGGGTCACACACTCATTTGTACCTAAGGAAAATATTATAGGGAGAGATGAAGATACTAAGACAATTATCCAGCTTTTGTTGGATCCCATCTCAACTGAGAATGTGTCAACCATTTCCATGGTTGGAATTGGAGGATTGGGGAAAACTGCACTTGCCCAACTCATATTCAACGATGAGGTGATTCAAAATCATTTTGAGTTGAAAATATGGACATGTGTCTCTAATGTATTTGAGTTGGATATAGTTGTTAAGAAAATCCTTCAATCCAAGCATAATGGGATAGAGCAATTGCAAAATGATCTTAGAAAAAAAGTAGATGGGAAGAAGTACCTACTTGTGTTGGATGACGTGTGGAATGAGGATCGAGAGAAGTGGCTTAGCTTGAAGGACTTGTTAATGGGTGGTGGAGAAGGTAGTAGAATACTGATTACCACTCGTAGTGAAACCGTTGCAACAACATCAGACACAGCTAAATCATACACCTTAAAGGGGTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

320

Amino Acids

36.26

Weight (kDa)

5.98

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 97 9e-22 Rx N-terminal domain
NB-ARC PF00931 174 - 309 5.6e-24 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 274
AciI CCGC 1 cut(s) 5
AclI AACGTT 1 cut(s) 24
AclWI GGATC 4 cut(s) 44, 536, 549, 828
AfaI GTAC 2 cut(s) 480, 788
AfiI CCNNNNNNNGG 1 cut(s) 948
AflIII ACRYGT 3 cut(s) 231, 671, 807
AgsI TTSAA 7 cut(s) 22, 628, 644, 661, 722, 844, 956
AjiI CACGTC 2 cut(s) 232, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AluBI AGCT 8 cut(s) 62, 112, 140, 292, 324, 532, 840, 929
AluI AGCT 8 cut(s) 62, 112, 140, 292, 324, 532, 840, 929
Alw26I GTCTC 2 cut(s) 113, 682
AlwI GGATC 4 cut(s) 44, 536, 549, 828
AlwNI CAGNNNCTG 1 cut(s) 128
Asp700I GAANNNNTTC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 649
AxyI CCTNAGG 1 cut(s) 483
BamHI GGATCC 1 cut(s) 541
BanI GGYRCC 1 cut(s) 274
BbsI GAAGAC 1 cut(s) 213
BccI CCATC 3 cut(s) 346, 554, 770
BcoDI GTCTC 2 cut(s) 113, 682
BfaI CTAG 3 cut(s) 50, 59, 321
BlpI GCTNAGC 2 cut(s) 156, 836
BmgBI CACGTC 2 cut(s) 232, 808
BmiI GGNNCC 4 cut(s) 55, 194, 276, 543
BmsI GCATC 1 cut(s) 407
BoxI GACNNNNGTC 1 cut(s) 674
BpiI GAAGAC 1 cut(s) 213
Bpu1102I GCTNAGC 2 cut(s) 156, 836
BpuEI CTTGAG 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 576
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 373, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 948
Bse21I CCTNAGG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 576
BseGI GGATG 2 cut(s) 244, 808
BseLI CCNNNNNNNGG 1 cut(s) 948
BseMII CTCAG 2 cut(s) 147, 546
BsgI GTGCAG 1 cut(s) 591
BshNI GGYRCC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 948
BsmAI GTCTC 2 cut(s) 113, 682
Bsp143I GATC 5 cut(s) 36, 72, 541, 757, 820
Bsp1720I GCTNAGC 2 cut(s) 156, 836
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 148, 547
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 4 cut(s) 55, 194, 276, 543
BspPI GGATC 4 cut(s) 44, 536, 549, 828
BspT107I GGYRCC 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 5 cut(s) 36, 72, 541, 757, 820
BssT1I CCWWGG 1 cut(s) 576
Bst4CI ACNGT 3 cut(s) 124, 189, 907
Bst6I CTCTTC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 8 cut(s) 156, 386, 418, 483, 516, 555, 761, 836
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 2 cut(s) 244, 808
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 5 cut(s) 39, 75, 544, 760, 823
BstMAI GTCTC 2 cut(s) 113, 682
BstMBI GATC 5 cut(s) 36, 72, 541, 757, 820
BstNSI RCATGY 1 cut(s) 675
BstPAI GACNNNNGTC 1 cut(s) 674
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 541
BstXI CCANNNNNNTGG 1 cut(s) 577
BstYI RGATCY 1 cut(s) 541
Bsu36I CCTNAGG 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 576
BtrI CACGTC 2 cut(s) 232, 808
BtsCI GGATG 2 cut(s) 244, 808
Cac8I GCNNGC 1 cut(s) 168
CaiI CAGNNNCTG 1 cut(s) 128
CciI TCATGA 1 cut(s) 430
CfoI GCGC 1 cut(s) 309
CseI GACGC 2 cut(s) 161, 218
Csp6I GTAC 2 cut(s) 479, 787
CviAII CATG 3 cut(s) 431, 577, 672
CviQI GTAC 2 cut(s) 479, 787
DdeI CTNAG 8 cut(s) 156, 386, 418, 483, 516, 555, 761, 836
DpnI GATC 5 cut(s) 38, 74, 543, 759, 822
DpnII GATC 5 cut(s) 36, 72, 541, 757, 820
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 576
Eco81I CCTNAGG 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 3 cut(s) 434, 580, 675
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FatI CATG 3 cut(s) 430, 576, 671
FokI GGATG 2 cut(s) 251, 815
FspBI CTAG 3 cut(s) 50, 59, 321
GlaI GCGC 1 cut(s) 308
HgaI GACGC 2 cut(s) 161, 218
HhaI GCGC 1 cut(s) 309
Hin1II CATG 3 cut(s) 434, 580, 675
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HinfI GANTC 3 cut(s) 132, 408, 640
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 1 cut(s) 567
Hpy188I TCNGA 2 cut(s) 77, 922
Hpy188III TCNNGA 4 cut(s) 106, 149, 431, 824
Hpy8I GTNNAC 1 cut(s) 567
HpyAV CCTTC 3 cut(s) 728, 838, 863
HpyCH4III ACNGT 3 cut(s) 124, 189, 907
HpyCH4IV ACGT 3 cut(s) 24, 231, 807
HpyCH4V TGCA 5 cut(s) 29, 398, 608, 751, 911
HpyF3I CTNAG 8 cut(s) 156, 386, 418, 483, 516, 555, 761, 836
HpySE526I ACGT 3 cut(s) 24, 231, 807
Hsp92II CATG 3 cut(s) 434, 580, 675
HspAI GCGC 1 cut(s) 307
Kzo9I GATC 5 cut(s) 36, 72, 541, 757, 820
LmnI GCTCC 2 cut(s) 59, 198
LpnPI CCDG 6 cut(s) 15, 114, 119, 291, 364, 542
LweI GCATC 1 cut(s) 407
MaeI CTAG 3 cut(s) 50, 59, 321
MaeII ACGT 3 cut(s) 24, 231, 807
MaeIII GTNAC 1 cut(s) 463
MalI GATC 5 cut(s) 38, 74, 543, 759, 822
MboI GATC 5 cut(s) 36, 72, 541, 757, 820
MboII GAAGA 6 cut(s) 10, 137, 218, 446, 521, 793
MfeI CAATTG 1 cut(s) 746
MflI RGATCY 1 cut(s) 541
MluCI AATT 4 cut(s) 213, 522, 585, 746
MmeI TCCRAC 5 cut(s) 216, 519, 562, 675, 780
MnlI CCTC 8 cut(s) 26, 39, 375, 385, 453, 584, 628, 811
MroXI GAANNNNTTC 1 cut(s) 12
MseI TTAA 5 cut(s) 246, 375, 708, 854, 944
MslI CAYNNNNRTG 1 cut(s) 575
MunI CAATTG 1 cut(s) 746
NcoI CCATGG 1 cut(s) 576
NdeII GATC 5 cut(s) 36, 72, 541, 757, 820
NlaIII CATG 3 cut(s) 434, 580, 675
NlaIV GGNNCC 4 cut(s) 55, 194, 276, 543
NmuCI GTSAC 1 cut(s) 463
NspI RCATGY 1 cut(s) 675
PagI TCATGA 1 cut(s) 430
PciI ACATGT 1 cut(s) 671
PdmI GAANNNNTTC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 132, 408, 640
PscI ACATGT 1 cut(s) 671
PshAI GACNNNNGTC 1 cut(s) 674
Psp1406I AACGTT 1 cut(s) 24
PspN4I GGNNCC 4 cut(s) 55, 194, 276, 543
PstNI CAGNNNCTG 1 cut(s) 128
PsuI RGATCY 1 cut(s) 541
RsaI GTAC 2 cut(s) 480, 788
RsaNI GTAC 2 cut(s) 479, 787
RseI CAYNNNNRTG 1 cut(s) 575
SaqAI TTAA 5 cut(s) 246, 375, 708, 854, 944
Sau3AI GATC 5 cut(s) 36, 72, 541, 757, 820
SfaNI GCATC 1 cut(s) 407
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 4 cut(s) 213, 522, 585, 746
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 493
SspMI CTAG 3 cut(s) 50, 59, 321
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 3 cut(s) 124, 189, 907
TaiI ACGT 3 cut(s) 27, 234, 810
TaqI TCGA 1 cut(s) 823
TasI AATT 4 cut(s) 213, 522, 585, 746
TfiI GAWTC 3 cut(s) 132, 408, 640
Tru1I TTAA 5 cut(s) 246, 375, 708, 854, 944
Tru9I TTAA 5 cut(s) 246, 375, 708, 854, 944
TseFI GTSAC 1 cut(s) 463
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 4 cut(s) 192, 234, 447, 522
XceI RCATGY 1 cut(s) 675
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 3 cut(s) 50, 59, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.