Rh4AG223400

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000725
Physical Location & Seq
Forward (+)
52292396 .. 52295757
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG223400.1

Sequence Viewer

Length: 2988 bp
ATGGTTGAAGCAGTTCCATTTGGCACTGCAGCTGACATACTCATCAAGCTCGGGTCTTCCACTTTCCGGGAGCTCGGGCTCATCTATGGCGTCAACAAGGAAATGAAGAAGCTTGAGAACACTCTCTCCACCATCAAAGCTGTTCTCTTAGACGCAGAGGAGAAGCAAGAGAAGAGCCATCTGGTGCAGGACTGGATCAGGAAGCTCAGCGAGGTTGTGTATGAGGCCGATGATTTGTTGGATGCATTTGAAACCAAAGCCAGGCAGCATGAGAATCATCAGGTGCGTGAGTTCTTTTCAAAGACACTTGCTTTTAAATACAAGATGGGTCGAAAACTGAGAGAAACTAGAGAGAGATTGGATGAGATTGCAGGGGATATTTCAAAGTTCAACTTCAGGGATAGGTCGTTGACAAACTCAGATATTGGGATTGAGATGAATAATAAGAGAGAAGAGACGCATTCGTTTATGGTGGAGTCGGCTGTAATTGGAAGAAAGAGAGAGGAGGAGGAAATGGTTAAGTTTTTGATATTCCCACCAAGTACTCAAGAAAATGTGTCTGTGACTGCTATTGTTGGTATTGGGGGACTTGGGAAGACCACCCTTGCCAAAATGTTGTATAATGATGATAGGGTAGTGAACTATTTTGAGATTAAGATGTGGGTGTGGGCTTATGGAAGTTTTGATTTATATTCAGTTGCGGAGAAAATTTTGAGATCTATTGCTACGAATGAACTAGAAGATTCGGAGTTCTATTACCAGCATATTGTGCTGCAGAAACAGTTTCTTATGGAGGAGTTGCAGATTCTGCTTCGGAAGAAATTGGATGGGAAGCGCTACCTCCTTGTTCTGGATGATGTGTGGAATGTGAAGCGCGAAAATTGGCTTCAGCTGAATGATTTGTTGAAAGGTGGTGCCAAGGGGAGTAAGATTGTTGTGACAACTCGTAGCACGAAAGTTACCGCGCTTATGGATGTTGATTTCCCTTTTGTGTTGCAAGATCTATCGGAGGAAGAGAGTTGGACATTGTTTAAGCAACTGGCTTTTAAAGATGGAAGGGAGGAGGAGCATCCGGAGTTGGTGCCAATAGGAAGAAGAATTGTGAAGAAATGCCGAGGTGTTCCTCTTGCAATAAGGACCCTAGGAAGCATGATGAGATACAAAGCTAATGAAGGAGAGTGGATTTCGATTCAAAATACAGAGATCTGGGAGTTACCAAAAGAGGAGAATGATGTTTTGAAGATACTGAAGTTAAGCTATGATCACATGCCAATTTATTTGAAACAATGCTTCTCCTACTGCGCATTGTTCCCCAAGGGATATGAAATTGAGAAAAAAATGTTGGTCGAACTTTGGATAGCTCAGGGTTACATTCACCTTCCTGGTAAAGAGAGGAACTTAGAAGAAATTGGTGACCTGTATTTCAAGGAATTGATAGAGAGATCATTATTTCAAGAGGTCAAGAAAGATGCCAATGGAAATGTAATTAGTTGTCATATGCATGATCTTATACACGATCTTTCACGGGCAGTTGCAGTATCTGAATGCTCAATTTGCAGTGTTGATGTTGGAGATATATCTGAAAGAGTTCATCATGTTTCATTTAGTTGTTATCTGCCTTCTTCATGGGAAGTTCCGGTAGCGTTGCTCAAAGCAAAGAAGACTAGGACATTTTTACTACCACTTCAGGATCAAGCATATAGAATTTCCAGTCACAATACAATCATTTCAAACTTGAGGTGCTTACGTGTGTTAGATCTACATAACCTAAGAATCGATGAGCTTTCAAATTCCGTAGGCAAGTTGACCCATCTTAGGTACCTTGACCTCTCTGGTAATGTTAGTATGACAGCACTCCCTGCTTCTATCAGCAATCTGCAGAACCTACAGACCCTTAAACTCAACAGGTGCTACAAACTTGAACACCTACCTAGAGATATTGGAAAGATGATCAGCCTTAGACATCTTGAGATCACTGGGTGCCGCAAATTGAATCACATGCCACCTGGGCTGGGAAAGTTGGTATCTCTTCAGACATTACCGTTATTTATCTTGGGCAGCAAAGCTCTCTTTTCCGGTGTGAGTGGTGGAATAGATGAGTTGAGCGGCTTGAACTGTCTTAAAGGGGTGCTTCATGTTGCCCATCTTGAACGTGTTGACAATGTCACAATAGATTCAAGGAGTTCAAATTTGAGCAGCAAGCAATATCTTGAATCCTTAAAACTAATTTGGTCCAGAGATGACATCAATGGAAGTAATGAAGATGACTTGGTGCTGGAAGGCCTTCAGCCCCATCAAAATTTGAAGGTACTGCATATAGCTGGGTATTGCGGCTTTCGATTCCCCAACTGGCTGATAAATAACGCTGCATCCTCTCTTCCAAGTCTAGTTGAGCTCACAATTGAGGGCTGTCTCAGATGCCAGCATCTTCCACCACTGGATCAACTGCCTTCTCTCAAGTTTCTCAAACTATATTCTCTAAGTTGCCTCGAGTACATCAACAACACCTCTTCTAGTTTGGTTGGTGGAGGACGAGAGCCTTTGTTTTTCCCATCATTGAAAGAGCTCACACTTTATGACTTGCCACTCTTGAAGGAGTGGCAAACCTGCAGTGATGAAAATGTGACAACAACAATGCCAAAGCAGCAGCGGCTTTCATTTCCTTCTCTCACCGAGTTGACTATCGTGGACACCCCTAACTTAATGTCCGTGCCGATGCTTCCACTAGTTGAAGAATTGCTTATGGACAGCGTCTCTGAGAAGTTGTTGCAGTCCATGTTGATGACAGCAGCCTCACAAAGCCAATCAAGCTCTTCCTCCCTTGCTAGATTAAAATTTCTGATCATCAAATCTTGCAATGATCTCGTCTCTTTCCCTGAAGATAGGTTAAATAACCTCACTTCTCTTAAGCTCCTAAAGATTATCGATTGTGGAAACTTGACATCTCTACCGCATAACCTGCACTGTGAATACGTACTTAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

995

Amino Acids

113.31

Weight (kDa)

6.27

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 15 - 97 3.2e-22 Rx N-terminal domain
NB-ARC PF00931 182 - 350 1e-30 NB-ARC domain
WHD_DRP PF23559 436 - 507 1.6e-26 Disease resistance protein Winged helix domain
LRR_14 PF23598 575 - 888 5.8e-34 Leucine-rich repeat region
LRR_8 PF13855 582 - 637 9.5e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 698 - 826 2.6e-34 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1303
Acc36I ACCTGC 2 cut(s) 2615, 2967
Acc65I GGTACC 1 cut(s) 1818
AccB1I GGYRCC 4 cut(s) 914, 1081, 1818, 1980
AccBSI CCGCTC 1 cut(s) 2106
AccII CGCG 2 cut(s) 876, 965
AccIII TCCGGA 1 cut(s) 1072
AciI CCGC 7 cut(s) 701, 963, 1984, 2106, 2331, 2650, 2951
AclWI GGATC 3 cut(s) 203, 1698, 2448
AcsI RAATTY 6 cut(s) 708, 1704, 1789, 2188, 2299, 2834
AcuI CTGAAG 7 cut(s) 379, 872, 1268, 1670, 2015, 2270, 2898
AcyI GRCGYC 1 cut(s) 90
AdeI CACNNNGTG 1 cut(s) 1980
AfaI GTAC 5 cut(s) 544, 1820, 2310, 2495, 2976
AfeI AGCGCT 1 cut(s) 836
AfiI CCNNNNNNNGG 5 cut(s) 66, 261, 850, 1815, 2012
AflII CTTAAG 1 cut(s) 2906
AflIII ACRYGT 2 cut(s) 1747, 2152
AhlI ACTAGT 1 cut(s) 2725
AjnI CCWGG 3 cut(s) 260, 1381, 2005
AjuI GAANNNNNNNTTGG 2 cut(s) 1325, 1357
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
Alw21I GWGCWC 3 cut(s) 75, 2397, 2568
Alw26I GTCTC 4 cut(s) 449, 2417, 2758, 2872
AlwI GGATC 3 cut(s) 203, 1698, 2448
AlwNI CAGNNNCTG 2 cut(s) 808, 1541
Ama87I CYCGRG 3 cut(s) 50, 74, 2489
Aor13HI TCCGGA 1 cut(s) 1072
Aor51HI AGCGCT 1 cut(s) 836
AoxI GGCC 2 cut(s) 225, 2281
ApeKI GCWGC 9 cut(s) 29, 265, 772, 2058, 2196, 2366, 2644, 2647, 2789
ApoI RAATTY 6 cut(s) 708, 1704, 1789, 2188, 2299, 2834
Asp700I GAANNNNTTC 3 cut(s) 12, 1587, 2283
Asp718I GGTACC 1 cut(s) 1818
AspA2I CCTAGG 1 cut(s) 1141
AspLEI GCGC 4 cut(s) 837, 876, 967, 1304
AspS9I GGNCC 2 cut(s) 1137, 2232
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 3 cut(s) 1367, 1424, 2662
AvaI CYCGRG 3 cut(s) 50, 74, 2489
AvaII GGWCC 2 cut(s) 1137, 2232
AvrII CCTAGG 1 cut(s) 1141
BaeI ACNNNNGTAYC 2 cut(s) 1235, 1268
BanI GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BanII GRGCYC 4 cut(s) 75, 81, 2397, 2568
BbsI GAAGAC 3 cut(s) 48, 602, 1667
Bbv12I GWGCWC 3 cut(s) 75, 2397, 2568
BbvI GCAGC 9 cut(s) 41, 277, 759, 2070, 2208, 2353, 2656, 2659, 2801
BccI CCATC 9 cut(s) 140, 186, 319, 821, 1046, 1818, 2151, 2301, 2560
BcgI CGANNNNNNTGC 2 cut(s) 2845, 2879
BciT130I CCWGG 3 cut(s) 262, 1383, 2007
BclI TGATCA 3 cut(s) 1261, 1950, 2841
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 4 cut(s) 449, 2417, 2758, 2872
BcuI ACTAGT 1 cut(s) 2725
BfaI CTAG 9 cut(s) 348, 737, 1142, 1665, 1932, 2387, 2514, 2726, 2826
BfmI CTRYAG 5 cut(s) 27, 773, 1877, 1886, 2608
BfoI RGCGCY 1 cut(s) 838
BfrI CTTAAG 1 cut(s) 2906
BfuAI ACCTGC 2 cut(s) 2615, 2967
BglI GCCNNNNNGGC 1 cut(s) 2008
BglII AGATCT 4 cut(s) 716, 1000, 1203, 1756
BlnI CCTAGG 1 cut(s) 1141
BlpI GCTNAGC 1 cut(s) 206
BmcAI AGTACT 1 cut(s) 544
Bme1390I CCNGG 4 cut(s) 68, 262, 1383, 2007
Bme18I GGWCC 2 cut(s) 1137, 2232
BmeT110I CYCGRG 3 cut(s) 50, 74, 2489
BmgT120I GGNCC 2 cut(s) 1137, 2232
BmiI GGNNCC 5 cut(s) 916, 1083, 1139, 1820, 1982
BmrFI CCNGG 4 cut(s) 68, 262, 1383, 2007
BmrI ACTGGG 1 cut(s) 1986
BmsI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2706
BmuI ACTGGG 1 cut(s) 1986
BpiI GAAGAC 3 cut(s) 48, 602, 1667
Bpu10I CCTNAGC 1 cut(s) 1362
Bpu1102I GCTNAGC 1 cut(s) 206
BpuEI CTTGAG 5 cut(s) 134, 531, 1756, 1988, 2441
BpuMI CCSGG 1 cut(s) 68
Bsa29I ATCGAT 2 cut(s) 1776, 2925
BsaAI YACGTR 2 cut(s) 1748, 2974
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 5 cut(s) 918, 1114, 1141, 1314, 2006
BsaWI WCCGGW 3 cut(s) 1072, 1636, 2075
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 261, 850, 1815, 2012
Bse1I ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
Bse3DI GCAATG 1 cut(s) 2863
BseAI TCCGGA 1 cut(s) 1072
BseBI CCWGG 3 cut(s) 262, 1383, 2007
BseCI ATCGAT 2 cut(s) 1776, 2925
BseDI CCNNGG 5 cut(s) 918, 1114, 1141, 1314, 2006
BseGI GGATG 7 cut(s) 247, 367, 832, 859, 979, 1069, 2369
BseLI CCNNNNNNNGG 5 cut(s) 66, 261, 850, 1815, 2012
BseMI GCAATG 1 cut(s) 2863
BseMII CTCAG 6 cut(s) 220, 329, 432, 1376, 2428, 2748
BseNI ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
BseRI GAGGAG 7 cut(s) 173, 518, 521, 809, 1076, 1079, 1238
BseXI GCAGC 9 cut(s) 41, 277, 759, 2070, 2208, 2353, 2656, 2659, 2801
BseYI CCCAGC 2 cut(s) 2011, 2321
BsgI GTGCAG 2 cut(s) 206, 2945
Bsh1236I CGCG 2 cut(s) 876, 965
BshFI GGCC 2 cut(s) 227, 2283
BshNI GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BshVI ATCGAT 2 cut(s) 1776, 2925
BsiHKAI GWGCWC 3 cut(s) 75, 2397, 2568
BsiHKCI CYCGRG 3 cut(s) 50, 74, 2489
BsiSI CCGG 4 cut(s) 67, 1073, 1637, 2076
BslFI GGGAC 1 cut(s) 600
BslI CCNNNNNNNGG 5 cut(s) 66, 261, 850, 1815, 2012
BsmAI GTCTC 4 cut(s) 449, 2417, 2758, 2872
BsmBI CGTCTC 3 cut(s) 449, 2758, 2872
BsmFI GGGAC 1 cut(s) 600
BsmI GAATGC 2 cut(s) 460, 1550
BsnI GGCC 2 cut(s) 227, 2283
BsoBI CYCGRG 3 cut(s) 50, 74, 2489
Bsp1286I GDGCHC 4 cut(s) 75, 81, 2397, 2568
Bsp13I TCCGGA 1 cut(s) 1072
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 7 cut(s) 701, 963, 1984, 2106, 2331, 2650, 2951
BspANI GGCC 2 cut(s) 227, 2283
BspCNI CTCAG 6 cut(s) 219, 330, 431, 1375, 2427, 2749
BspDI ATCGAT 2 cut(s) 1776, 2925
BspEI TCCGGA 1 cut(s) 1072
BspFNI CGCG 2 cut(s) 876, 965
BspLI GGNNCC 5 cut(s) 916, 1083, 1139, 1820, 1982
BspMAI CTGCAG 4 cut(s) 31, 777, 1881, 2612
BspMI ACCTGC 2 cut(s) 2615, 2967
BspPI GGATC 3 cut(s) 203, 1698, 2448
BspQI GCTCTTC 2 cut(s) 167, 2818
BspT107I GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BspTI CTTAAG 1 cut(s) 2906
BsrBI CCGCTC 1 cut(s) 2106
BsrDI GCAATG 1 cut(s) 2863
BsrI ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
BssECI CCNNGG 5 cut(s) 918, 1114, 1141, 1314, 2006
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 3 cut(s) 918, 1141, 1314
Bst2UI CCWGG 3 cut(s) 262, 1383, 2007
Bst4CI ACNGT 4 cut(s) 783, 2043, 2117, 2966
Bst6I CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2515, 2818
BstACI GRCGYC 1 cut(s) 90
BstAFI CTTAAG 1 cut(s) 2906
BstAPI GCANNNNNTGC 4 cut(s) 769, 808, 1859, 2959
BstBAI YACGTR 2 cut(s) 1748, 2974
BstC8I GCNNGC 2 cut(s) 2201, 2423
BstEII GGTNACC 1 cut(s) 1412
BstF5I GGATG 7 cut(s) 247, 367, 832, 859, 979, 1069, 2369
BstFNI CGCG 2 cut(s) 876, 965
BstH2I RGCGCY 1 cut(s) 838
BstHHI GCGC 4 cut(s) 837, 876, 967, 1304
BstMAI GTCTC 4 cut(s) 449, 2417, 2758, 2872
BstMWI GCNNNNNNNGC 9 cut(s) 769, 808, 1554, 1859, 2008, 2644, 2650, 2808, 2959
BstNI CCWGG 3 cut(s) 262, 1383, 2007
BstNSI RCATGY 2 cut(s) 1270, 2002
BstPI GGTNACC 1 cut(s) 1412
BstSCI CCNGG 4 cut(s) 66, 260, 1381, 2005
BstSFI CTRYAG 5 cut(s) 27, 773, 1877, 1886, 2608
BstSNI TACGTA 1 cut(s) 2974
BstUI CGCG 2 cut(s) 876, 965
BstV1I GCAGC 9 cut(s) 41, 277, 759, 2070, 2208, 2353, 2656, 2659, 2801
BstV2I GAAGAC 3 cut(s) 48, 602, 1667
BstX2I RGATCY 4 cut(s) 716, 1000, 1203, 1756
BstYI RGATCY 4 cut(s) 716, 1000, 1203, 1756
Bsu15I ATCGAT 2 cut(s) 1776, 2925
BsuRI GGCC 2 cut(s) 227, 2283
BsuTUI ATCGAT 2 cut(s) 1776, 2925
BtsCI GGATG 7 cut(s) 247, 367, 832, 859, 979, 1069, 2369
BtsI GCAGTG 3 cut(s) 24, 1564, 2617
BtsIMutI CAGTG 6 cut(s) 24, 1564, 1974, 2435, 2617, 2962
BveI ACCTGC 2 cut(s) 2615, 2967
Cac8I GCNNGC 2 cut(s) 2201, 2423
CaiI CAGNNNCTG 2 cut(s) 808, 1541
CfoI GCGC 4 cut(s) 837, 876, 967, 1304
Cfr13I GGNCC 2 cut(s) 1137, 2232
ClaI ATCGAT 2 cut(s) 1776, 2925
CseI GACGC 4 cut(s) 79, 161, 466, 2740
Csp6I GTAC 5 cut(s) 543, 1819, 2309, 2494, 2975
CviAII CATG 9 cut(s) 269, 1150, 1267, 1502, 1595, 1626, 1999, 2135, 2776
CviQI GTAC 5 cut(s) 543, 1819, 2309, 2494, 2975
DraI TTTAAA 2 cut(s) 316, 1048
DraIII CACNNNGTG 1 cut(s) 1980
Eam1104I CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2515, 2818
EarI CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2515, 2818
Ecl136II GAGCTC 3 cut(s) 73, 2395, 2566
Eco105I TACGTA 1 cut(s) 2974
Eco130I CCWWGG 3 cut(s) 918, 1141, 1314
Eco147I AGGCCT 1 cut(s) 2283
Eco24I GRGCYC 4 cut(s) 75, 81, 2397, 2568
Eco47I GGWCC 2 cut(s) 1137, 2232
Eco47III AGCGCT 1 cut(s) 836
Eco53kI GAGCTC 3 cut(s) 73, 2395, 2566
Eco57I CTGAAG 7 cut(s) 379, 872, 1268, 1670, 2015, 2270, 2898
Eco88I CYCGRG 3 cut(s) 50, 74, 2489
Eco91I GGTNACC 1 cut(s) 1412
EcoICRI GAGCTC 3 cut(s) 73, 2395, 2566
EcoO109I RGGNCCY 1 cut(s) 1137
EcoO65I GGTNACC 1 cut(s) 1412
EcoRII CCWGG 3 cut(s) 260, 1381, 2005
EcoT14I CCWWGG 3 cut(s) 918, 1141, 1314
EcoT22I ATGCAT 2 cut(s) 247, 1503
EcoT38I GRGCYC 4 cut(s) 75, 81, 2397, 2568
ErhI CCWWGG 3 cut(s) 918, 1141, 1314
Esp3I CGTCTC 3 cut(s) 449, 2758, 2872
FaeI CATG 9 cut(s) 272, 1153, 1270, 1505, 1598, 1629, 2002, 2138, 2779
FalI AAGNNNNNCTT 6 cut(s) 377, 409, 2115, 2147, 2724, 2756
FaqI GGGAC 1 cut(s) 600
FatI CATG 9 cut(s) 268, 1149, 1266, 1501, 1594, 1625, 1998, 2134, 2775
FauNDI CATATG 1 cut(s) 1497
FbaI TGATCA 3 cut(s) 1261, 1950, 2841
FokI GGATG 7 cut(s) 254, 374, 839, 866, 986, 1056, 2356
FriOI GRGCYC 4 cut(s) 75, 81, 2397, 2568
FspBI CTAG 9 cut(s) 348, 737, 1142, 1665, 1932, 2387, 2514, 2726, 2826
FspI TGCGCA 1 cut(s) 1303
GlaI GCGC 4 cut(s) 836, 875, 966, 1303
GsaI CCCAGC 2 cut(s) 2015, 2325
HaeII RGCGCY 1 cut(s) 838
HaeIII GGCC 2 cut(s) 227, 2283
HapII CCGG 4 cut(s) 67, 1073, 1637, 2076
HgaI GACGC 4 cut(s) 79, 161, 466, 2740
HhaI GCGC 4 cut(s) 837, 876, 967, 1304
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 9 cut(s) 272, 1153, 1270, 1505, 1598, 1629, 2002, 2138, 2779
Hin6I GCGC 4 cut(s) 835, 874, 965, 1302
HinP1I GCGC 4 cut(s) 835, 874, 965, 1302
HincII GTYRAC 5 cut(s) 94, 411, 1806, 2158, 2679
HindII GTYRAC 5 cut(s) 94, 411, 1806, 2158, 2679
HindIII AAGCTT 1 cut(s) 110
HpaII CCGG 4 cut(s) 67, 1073, 1637, 2076
HphI GGTGA 3 cut(s) 1367, 1424, 2662
Hpy166II GTNNAC 7 cut(s) 94, 411, 640, 1806, 2158, 2679, 2689
Hpy8I GTNNAC 7 cut(s) 94, 411, 640, 1806, 2158, 2679, 2689
HpyCH4III ACNGT 4 cut(s) 783, 2043, 2117, 2966
HpyCH4IV ACGT 3 cut(s) 1747, 2152, 2973
HpyF10VI GCNNNNNNNGC 9 cut(s) 769, 808, 1554, 1859, 2008, 2644, 2650, 2808, 2959
HpySE526I ACGT 3 cut(s) 1747, 2152, 2973
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 9 cut(s) 272, 1153, 1270, 1505, 1598, 1629, 2002, 2138, 2779
HspAI GCGC 4 cut(s) 835, 874, 965, 1302
Kpn2I TCCGGA 1 cut(s) 1072
KpnI GGTACC 1 cut(s) 1822
Ksp22I TGATCA 3 cut(s) 1261, 1950, 2841
LguI GCTCTTC 2 cut(s) 167, 2818
LmnI GCTCC 3 cut(s) 70, 1066, 2916
Lsp1109I GCAGC 9 cut(s) 41, 277, 759, 2070, 2208, 2353, 2656, 2659, 2801
LweI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2706
MaeI CTAG 9 cut(s) 348, 737, 1142, 1665, 1932, 2387, 2514, 2726, 2826
MaeII ACGT 3 cut(s) 1747, 2152, 2973
MaeIII GTNAC 9 cut(s) 562, 937, 958, 1212, 1367, 1412, 1712, 2164, 2623
MbiI CCGCTC 1 cut(s) 2106
MfeI CAATTG 1 cut(s) 2400
MflI RGATCY 4 cut(s) 716, 1000, 1203, 1756
MhlI GDGCHC 4 cut(s) 75, 81, 2397, 2568
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 3 cut(s) 219, 1001, 1549
Mph1103I ATGCAT 2 cut(s) 247, 1503
MroI TCCGGA 1 cut(s) 1072
MroXI GAANNNNTTC 3 cut(s) 12, 1587, 2283
MslI CAYNNNNRTG 2 cut(s) 1500, 2780
MspA1I CMGCKG 3 cut(s) 32, 892, 2650
MspCI CTTAAG 1 cut(s) 2906
MspI CCGG 4 cut(s) 67, 1073, 1637, 2076
MspR9I CCNGG 4 cut(s) 68, 262, 1383, 2007
MunI CAATTG 1 cut(s) 2400
Mva1269I GAATGC 2 cut(s) 460, 1550
MvaI CCWGG 3 cut(s) 262, 1383, 2007
MvnI CGCG 2 cut(s) 876, 965
MwoI GCNNNNNNNGC 9 cut(s) 769, 808, 1554, 1859, 2008, 2644, 2650, 2808, 2959
NciI CCSGG 1 cut(s) 68
NdeI CATATG 1 cut(s) 1497
NlaIII CATG 9 cut(s) 272, 1153, 1270, 1505, 1598, 1629, 2002, 2138, 2779
NlaIV GGNNCC 5 cut(s) 916, 1083, 1139, 1820, 1982
NmeAIII GCCGAG 1 cut(s) 1139
NmuCI GTSAC 6 cut(s) 562, 937, 1412, 1712, 2164, 2623
NsbI TGCGCA 1 cut(s) 1303
NsiI ATGCAT 2 cut(s) 247, 1503
NspI RCATGY 2 cut(s) 1270, 2002
PaeR7I CTCGAG 1 cut(s) 2489
PceI AGGCCT 1 cut(s) 2283
PciSI GCTCTTC 2 cut(s) 167, 2818
PctI GAATGC 2 cut(s) 460, 1550
PdmI GAANNNNTTC 3 cut(s) 12, 1587, 2283
PfeI GAWTC 9 cut(s) 274, 743, 805, 1189, 1773, 1993, 2174, 2213, 2340
PflFI GACNNNGTC 2 cut(s) 2162, 2750
PfoI TCCNGGA 1 cut(s) 66
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
Ppu21I YACGTR 2 cut(s) 1748, 2974
PpuMI RGGWCCY 1 cut(s) 1137
Psp124BI GAGCTC 3 cut(s) 75, 2397, 2568
Psp5II RGGWCCY 1 cut(s) 1137
Psp6I CCWGG 3 cut(s) 260, 1381, 2005
PspEI GGTNACC 1 cut(s) 1412
PspFI CCCAGC 2 cut(s) 2011, 2321
PspGI CCWGG 3 cut(s) 260, 1381, 2005
PspN4I GGNNCC 5 cut(s) 916, 1083, 1139, 1820, 1982
PspPI GGNCC 2 cut(s) 1137, 2232
PspPPI RGGWCCY 1 cut(s) 1137
PspXI VCTCGAGB 1 cut(s) 2489
PstI CTGCAG 4 cut(s) 31, 777, 1881, 2612
PstNI CAGNNNCTG 2 cut(s) 808, 1541
PsuI RGATCY 4 cut(s) 716, 1000, 1203, 1756
PsyI GACNNNGTC 2 cut(s) 2162, 2750
PvuII CAGCTG 2 cut(s) 32, 892
RsaI GTAC 5 cut(s) 544, 1820, 2310, 2495, 2976
RsaNI GTAC 5 cut(s) 543, 1819, 2309, 2494, 2975
RseI CAYNNNNRTG 2 cut(s) 1500, 2780
SacI GAGCTC 3 cut(s) 75, 2397, 2568
SapI GCTCTTC 2 cut(s) 167, 2818
Sau96I GGNCC 2 cut(s) 1137, 2232
ScaI AGTACT 1 cut(s) 544
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 4 cut(s) 68, 262, 1383, 2007
SduI GDGCHC 4 cut(s) 75, 81, 2397, 2568
SfaNI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2706
SfcI CTRYAG 5 cut(s) 27, 773, 1877, 1886, 2608
Sfr274I CTCGAG 1 cut(s) 2489
SinI GGWCC 2 cut(s) 1137, 2232
SlaI CTCGAG 1 cut(s) 2489
SmiMI CAYNNNNRTG 2 cut(s) 1500, 2780
SmlI CTYRAG 7 cut(s) 113, 546, 1735, 1967, 2456, 2489, 2906
SmoI CTYRAG 7 cut(s) 113, 546, 1735, 1967, 2456, 2489, 2906
SnaBI TACGTA 1 cut(s) 2974
SpeI ACTAGT 1 cut(s) 2725
SseBI AGGCCT 1 cut(s) 2283
SsiI CCGC 7 cut(s) 701, 963, 1984, 2106, 2331, 2650, 2951
SspMI CTAG 9 cut(s) 348, 737, 1142, 1665, 1932, 2387, 2514, 2726, 2826
SstI GAGCTC 3 cut(s) 75, 2397, 2568
StuI AGGCCT 1 cut(s) 2283
StyD4I CCNGG 4 cut(s) 66, 260, 1381, 2005
StyI CCWWGG 3 cut(s) 918, 1141, 1314
TaaI ACNGT 4 cut(s) 783, 2043, 2117, 2966
TaiI ACGT 3 cut(s) 1750, 2155, 2976
TaqI TCGA 7 cut(s) 331, 1187, 1347, 1776, 2338, 2490, 2925
TatI WGTACW 2 cut(s) 542, 2493
TauI GCSGC 4 cut(s) 1986, 2109, 2334, 2653
TfiI GAWTC 9 cut(s) 274, 743, 805, 1189, 1773, 1993, 2174, 2213, 2340
TscAI CASTG 6 cut(s) 31, 1564, 1981, 2442, 2617, 2969
TseFI GTSAC 6 cut(s) 562, 937, 1412, 1712, 2164, 2623
TseI GCWGC 9 cut(s) 29, 265, 772, 2058, 2196, 2366, 2644, 2647, 2789
Tsp45I GTSAC 6 cut(s) 562, 937, 1412, 1712, 2164, 2623
TspGWI ACGGA 2 cut(s) 1783, 2698
TspRI CASTG 6 cut(s) 31, 1564, 1981, 2442, 2617, 2969
Tth111I GACNNNGTC 2 cut(s) 2162, 2750
Vha464I CTTAAG 1 cut(s) 2906
VpaK11BI GGWCC 2 cut(s) 1137, 2232
XapI RAATTY 6 cut(s) 708, 1704, 1789, 2188, 2299, 2834
XceI RCATGY 2 cut(s) 1270, 2002
XhoI CTCGAG 1 cut(s) 2489
XmaJI CCTAGG 1 cut(s) 1141
XmnI GAANNNNTTC 3 cut(s) 12, 1587, 2283
XspI CTAG 9 cut(s) 348, 737, 1142, 1665, 1932, 2387, 2514, 2726, 2826
ZrmI AGTACT 1 cut(s) 544
Zsp2I ATGCAT 2 cut(s) 247, 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.