MD03G1280200.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000725
Physical Location & Seq
Reverse (-)
36151872 .. 36156419
4548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1280200.v1.1.491

Sequence Viewer

Length: 3378 bp
ATGGCGGAAGGAGTTCTCTTCAACGTTGCAGCACGGATCATCGGAAGGCTAGGCTCCCTTGCTTTCCAAGAGATCGGATTGATTTGGGGTGTCCAAGATGAGCTCCACAAGCTCCTGGAGATAGTTGCCGGATTCCAAGCTGTTCTTCTTGATGCTGAGAAAAAGCAAACCATCAATGAAGTCAAAGTGTGGCTCGAAAGCGTAGAAGATGCAGTTTATGAAGCCGATGACCTGCTGGATGAGTTTAATACTGAAGCGCAGCAGAGACAAATGATGTGTGGCAATACTAAGATGTCAAAGAAGGTACGCCTCTTCTTCTCTAGCTCAAATCAACTTGCTTTTCGGTTAAAGATGGGTCATAAGATAAAAGATCTTAACAAGAGGCTTCGTGAGGTTGCATCCCGTAGAGCCTTTCCCTTAGAAGTAAATTGTGAAGATACACGATTTATAATTAGAGAGAGGATCACTCACTCATTTGTCCCTAAGGAAAATATTATAGGGAGAGATGAAGATAGAAAGGCAATTATTCAACTTTTGTTGGATCCCATCTCAACTGAGAATGTGTCCACCATTTCCATAGTTGGATTTGGAGGATTGGGGAAAACTGGTCTTGCCCAACTCATATTCAACGATGAGGTGATTCAAAATCATTTTGAGTTGAAAATATGGTCATGTGTCTCTAATGTATTTGAGTTGGATATACTTGTTAAGAAAATACTAAAAGTTGACAAGATTGATATGGATGAGCTGCAAAATGATCTTAGAAGAAAAGTAGATGGAAAGAAATACCTAATTGTGTTGGATGATGTGTGGAATGAGAATAGGGAGAAATGGCTTACCTTGAAGTACTTGTTAATGGGGGGTGGAAAAGGTAGTAGAATACTAATAACCACTCGTAGTGAAACAGTTGCGAAGATATCAGACACAGCTAAACCGTACACCTTAAGGGGTTTGAATCAAGAGGACAGTTGGTCTTTATTTAAGGAAATGGCTTTTAAAGATGGAAAAGAGCCAGAGAATTCAACAATTAAGGCAATTGGGAAGGAGGTTGCAAGAAAATGTCAGGGAGTTCCACTCGCTATAAGGACAATAGGTGGGATGTTGCACACCAAATATCACGAAACAGAATGGTTGAATTTCAAAGAAAAGAAACTTTCAAGAATAAATCAGGAAGAAAATAAAATTTTACCAACACTTAAACTAAGTTATGATGTGCTCCCATCACATTTGAAGCATTGTTTTGCTTACTGTAGCTTGTTCCCACCTGATTATGAGATCTCTGTACCGATGTTGATTAGACTTTGGGTGGCGCAAGGGTTCATTAAGTCATCAGGTGAAAACGAGAGTTTGGAGGATGTTGCGTATGAGTATTATATGGAATTGTTGTGCAGATCGTTTTTTCAAGAAGAAGAAAAAGATGAGTTTGGTGTAATAACAAGTTGTAAAATGCACGATCTCATGAATGAACTTGCAATCTTAGTGTCGGGGGTCAGAAGCGCCATAGTTGATCGGAACCAAAAGAAATTTCATGAAAAGCTTCGTCATGTATCTTTCAATTTTCATGTTGATCTGTCGAAATGGGAAGTGCCAACTTCCTTGCTAAATGCAAAAAAGATACGAACCTTTCTTCTTCCTTGCCAAAAGTTTAGAGGTTTTTCAGTAGATAATTCATTTTCTGCTACAATTATCTCAAATTTTAAGTCACTGCGTATGTTGAGTCTCAAGCGCATAGGAAATGAAAAATTACCAAATTGTCTTAAAAAAGTGAAACATTTGAGATATCTTGATCTTAGTGATAATGATGGCATCAAGAGACTTCCAGATTGGATAGTTGGACTTTCGAATTTGGAAACACTAGATCTCAGTCGGTGTGATAGGCTTGTGGAATTGCCTAGAGACATAAAAAAAATGATCAACTTAAGGCATCTCCTCTTGCAAGGCTGTAATGGATTGACTCGAATGCCACGTGGATTGGGGGAGCTGAATGGTCTTCGTACATTGAATAGATTTGTTTTGAGCGAAAACAATTCTTTGTTGAGGGACAGTGCTGCGGGTCTTGGTGAACTGGGAAGGCTTAAGGAATTAAGGGGATGGTTAGAAATTAAATACTTGAGGCACGAGAAAGATGTGATGTCAGAATCAAATGTTGGTACGCCTCTCAAGGAGAAACAGCATCTCCATTCGTTGCATTTATGGTGGAAATATGGAGAAGATGTAAACGCAATTGATGAGAAGGTTATTATAATGTCAATGGAAGTATTGCAACCCCATTCAAATCTAAAGAAGTTGTCCGTGTGGTATTATGGTGGTGTGAGGTTTCCGATTTGGTTTTCTTCTCTCATAAATCTTGTTGATCTCAGATTGTGGGGGTGTCATAGATGCCAACATCTTCCACCCTTGGATCATTTCCCATCCCTTAAGTATCTTGACCTTATTGGGTTTGAGAAGTTGGAGTACATATCAGACGATACCAGCAGCGGCAATAGTATGAGTGATGAGATGATGACGTCCCTGGAGGGCCTCTGGGTAGAAAGTTGCCCTGTTCTGAAGGGATGGTGGAGGGCGCACACTCATAACAATGCTTCTTCTTCTTCATCAACGGAAAATCTGTCTTTGCCTTCTTTTCCCGGTCTTTCTACATTGTGGATCAGTAATTGTCCTAATCTAACTTGCATGCCTCTGTATCCAAATGTGGAGAGAATAGATCTCAATGGGTGCAGCTCGAAGGTTGTTGATTCCTTGTTTGTTAGAGGAGCATCTGATATTACACATGATGTTGGTGTTGATGTCTCTGCCTCTTCTTCTTCCCCTCATTTCTTCAAATTAACACATCTGTCACTCAGTAATATTGAGGATTTGGAATGTGTAACGTCACTAGGAATGGGCAACGTCCCATCACTCCAATCGCTTGACATTAAAGATTGCCCAAATTTGGCATTACTACCATATCATGGGATGGGCAACCTCGCATCCTTTCGGGAGCTTACAGTTACAAATTGCTCCAATTTGACATCAATGTTAGAAGGGATCGCCAATCTTACATCACTTCAGAAGCTTGCAATTGGAGAATACTCTGATCTGACATTACCGCCAGAAGTGGTCGTCAACCTCCCATCACTCCAATCGCTTACGATTGTATCTTTCCCAAATTTGGTATCACTACCGGAAGAAATAAGCAATCTCACGTTACTGCAGAACCTTGAAATTCAAAGTTGCCCTAATTTGGCATCACTGCCAGAAGGGATTCGTCGACTCCCCAACTTAAATACATTGGGAATTTGGAGATGCCCCGTGTTAAGCGAAAGATGCGAGAAGGAAACAGGGGAGGACTGGCCTAAGATTGCTCACATCCCATCCATTTCTATTCATAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1126

Amino Acids

127.75

Weight (kDa)

6.54

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 97 2.9e-19 Rx N-terminal domain
NB-ARC PF00931 175 - 334 9.5e-30 NB-ARC domain
WHD_DRP PF23559 419 - 491 4.4e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 561 - 812 1.5e-27 Leucine-rich repeat region
LRR_8 PF13855 586 - 624 4.3e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 686 - 812 1.6e-29 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 873 - 1000 4.7e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 964 - 1122 3.3e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 966 - 1096 8.5e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 449, 2243
AatII GACGTC 1 cut(s) 2510
Acc36I ACCTGC 1 cut(s) 240
AccB7I CCANNNNNTGG 1 cut(s) 2951
AccI GTMKAC 1 cut(s) 3250
AciI CCGC 4 cut(s) 5, 2051, 2478, 3089
AclI AACGTT 1 cut(s) 24
AclWI GGATC 7 cut(s) 44, 470, 536, 549, 2409, 2654, 3035
AcuI CTGAAG 3 cut(s) 273, 2567, 3032
AcvI CACGTG 1 cut(s) 1967
AcyI GRCGYC 1 cut(s) 2507
AfaI GTAC 7 cut(s) 306, 848, 938, 1284, 1996, 2152, 2456
AfiI CCNNNNNNNGG 4 cut(s) 2932, 2951, 3151, 3223
AflII CTTAAG 4 cut(s) 943, 1918, 2075, 2417
AhdI GACNNNNNGTC 1 cut(s) 970
AjnI CCWGG 2 cut(s) 114, 2511
AjuI GAANNNNNNNTTGG 6 cut(s) 129, 161, 1509, 1541, 2130, 2162
Alw21I GWGCWC 2 cut(s) 105, 1218
Alw26I GTCTC 6 cut(s) 259, 682, 1724, 1807, 1890, 2794
AlwI GGATC 7 cut(s) 44, 470, 536, 549, 2409, 2654, 3035
AoxI GGCC 2 cut(s) 2518, 3332
ApeKI GCWGC 6 cut(s) 29, 259, 748, 2048, 2475, 2718
ArsI GACNNNNNNTTYG 2 cut(s) 1685, 1717
Asp700I GAANNNNTTC 3 cut(s) 12, 1536, 3243
AspLEI GCGC 5 cut(s) 259, 1312, 1499, 1728, 2566
AspS9I GGNCC 1 cut(s) 2518
AsuC2I CCSGG 1 cut(s) 2628
AsuHPI GGTGA 3 cut(s) 649, 1346, 2072
AsuII TTCGAA 1 cut(s) 1841
AxyI CCTNAGG 1 cut(s) 483
BaeI ACNNNNGTAYC 2 cut(s) 3120, 3153
BamHI GGATCC 1 cut(s) 541
BanII GRGCYC 1 cut(s) 105
BarI GAAGNNNNNNTAC 2 cut(s) 2438, 2470
BauI CACGAG 1 cut(s) 2117
BbrPI CACGTG 1 cut(s) 1967
BbsI GAAGAC 1 cut(s) 1982
Bbv12I GWGCWC 2 cut(s) 105, 1218
BbvI GCAGC 6 cut(s) 41, 271, 735, 2035, 2487, 2730
BciT130I CCWGG 2 cut(s) 116, 2513
BciVI GTATCC 1 cut(s) 2694
BclI TGATCA 1 cut(s) 1911
BcnI CCSGG 1 cut(s) 2628
BcoDI GTCTC 6 cut(s) 259, 682, 1724, 1807, 1890, 2794
BfaI CTAG 5 cut(s) 50, 321, 1856, 1893, 2876
BfmI CTRYAG 2 cut(s) 1249, 3191
BfoI RGCGCY 1 cut(s) 1500
BfrI CTTAAG 4 cut(s) 943, 1918, 2075, 2417
BfuAI ACCTGC 1 cut(s) 240
BfuI GTATCC 1 cut(s) 2694
BglII AGATCT 4 cut(s) 370, 1275, 1858, 2704
BisI GCNGC 7 cut(s) 30, 260, 749, 2049, 2476, 2479, 2719
BlsI GCNGC 7 cut(s) 31, 261, 750, 2050, 2477, 2480, 2720
BmcAI AGTACT 1 cut(s) 848
Bme1390I CCNGG 3 cut(s) 116, 2513, 2628
BmeRI GACNNNNNGTC 1 cut(s) 970
BmgT120I GGNCC 1 cut(s) 2518
BmiI GGNNCC 3 cut(s) 55, 543, 1514
BmrFI CCNGG 3 cut(s) 116, 2513, 2628
BmrI ACTGGG 1 cut(s) 2075
BmuI ACTGGG 1 cut(s) 2075
BpiI GAAGAC 1 cut(s) 1982
BplI GAGNNNNNCTC 4 cut(s) 451, 483, 1059, 1091
BpmI CTGGAG 2 cut(s) 137, 2534
Bpu14I TTCGAA 1 cut(s) 1841
BpuEI CTTGAG 3 cut(s) 1706, 2131, 2144
BpuMI CCSGG 1 cut(s) 2628
BsaAI YACGTR 1 cut(s) 1967
BsaHI GRCGYC 1 cut(s) 2507
BsaJI CCNNGG 2 cut(s) 2397, 2511
BsaWI WCCGGW 1 cut(s) 3163
BsaXI ACNNNNNCTCC 4 cut(s) 2160, 2190, 2199, 2229
Bsc4I CCNNNNNNNGG 4 cut(s) 2932, 2951, 3151, 3223
Bse1I ACTGG 3 cut(s) 610, 2070, 3335
Bse21I CCTNAGG 1 cut(s) 483
BseBI CCWGG 2 cut(s) 116, 2513
BseDI CCNNGG 2 cut(s) 2397, 2511
BseLI CCNNNNNNNGG 4 cut(s) 2932, 2951, 3151, 3223
BseMII CTCAG 5 cut(s) 147, 546, 1876, 2371, 2854
BseNI ACTGG 3 cut(s) 610, 2070, 3335
BseRI GAGGAG 2 cut(s) 1919, 2766
BseXI GCAGC 6 cut(s) 41, 271, 735, 2035, 2487, 2730
BsgI GTGCAG 2 cut(s) 1408, 2737
BshFI GGCC 2 cut(s) 2520, 3334
BsiHKAI GWGCWC 2 cut(s) 105, 1218
BsiSI CCGG 3 cut(s) 129, 2628, 3164
BslFI GGGAC 4 cut(s) 464, 2054, 2494, 2876
BslI CCNNNNNNNGG 4 cut(s) 2932, 2951, 3151, 3223
BsmAI GTCTC 6 cut(s) 259, 682, 1724, 1807, 1890, 2794
BsmFI GGGAC 4 cut(s) 464, 2054, 2494, 2876
BsmI GAATGC 1 cut(s) 1965
BsnI GGCC 2 cut(s) 2520, 3334
Bsp119I TTCGAA 1 cut(s) 1841
Bsp1286I GDGCHC 2 cut(s) 105, 1218
BspACI CCGC 4 cut(s) 5, 2051, 2478, 3089
BspANI GGCC 2 cut(s) 2520, 3334
BspCNI CTCAG 5 cut(s) 148, 547, 1875, 2370, 2853
BspHI TCATGA 2 cut(s) 1458, 1528
BspLI GGNNCC 3 cut(s) 55, 543, 1514
BspMAI CTGCAG 1 cut(s) 3195
BspMI ACCTGC 1 cut(s) 240
BspPI GGATC 7 cut(s) 44, 470, 536, 549, 2409, 2654, 3035
BspT104I TTCGAA 1 cut(s) 1841
BspTI CTTAAG 4 cut(s) 943, 1918, 2075, 2417
BsrI ACTGG 3 cut(s) 610, 2070, 3335
BssECI CCNNGG 2 cut(s) 2397, 2511
BssNI GRCGYC 1 cut(s) 2507
BssSI CACGAG 1 cut(s) 2117
BssT1I CCWWGG 1 cut(s) 2397
Bst2BI CACGAG 1 cut(s) 2117
Bst2UI CCWGG 2 cut(s) 116, 2513
Bst4CI ACNGT 6 cut(s) 907, 936, 968, 1250, 2045, 2989
Bst6I CTCTTC 3 cut(s) 23, 317, 2803
BstACI GRCGYC 1 cut(s) 2507
BstAFI CTTAAG 4 cut(s) 943, 1918, 2075, 2417
BstBAI YACGTR 1 cut(s) 1967
BstBI TTCGAA 1 cut(s) 1841
BstC8I GCNNGC 2 cut(s) 2675, 3057
BstH2I RGCGCY 1 cut(s) 1500
BstHHI GCGC 5 cut(s) 259, 1312, 1499, 1728, 2566
BstMAI GTCTC 6 cut(s) 259, 682, 1724, 1807, 1890, 2794
BstMWI GCNNNNNNNGC 2 cut(s) 109, 3306
BstNI CCWGG 2 cut(s) 116, 2513
BstNSI RCATGY 1 cut(s) 2677
BstSCI CCNGG 3 cut(s) 114, 2511, 2626
BstSFI CTRYAG 2 cut(s) 1249, 3191
BstV1I GCAGC 6 cut(s) 41, 271, 735, 2035, 2487, 2730
BstV2I GAAGAC 1 cut(s) 1982
BstX2I RGATCY 5 cut(s) 370, 541, 1275, 1858, 2704
BstYI RGATCY 5 cut(s) 370, 541, 1275, 1858, 2704
Bsu36I CCTNAGG 1 cut(s) 483
BsuI GTATCC 1 cut(s) 2694
BsuRI GGCC 2 cut(s) 2520, 3334
BtsI GCAGTG 2 cut(s) 1703, 3230
BtsIMutI CAGTG 3 cut(s) 1703, 2050, 3230
BveI ACCTGC 1 cut(s) 240
Cac8I GCNNGC 2 cut(s) 2675, 3057
CciI TCATGA 2 cut(s) 1458, 1528
CfoI GCGC 5 cut(s) 259, 1312, 1499, 1728, 2566
Cfr13I GGNCC 1 cut(s) 2518
Csp6I GTAC 7 cut(s) 305, 847, 937, 1283, 1995, 2151, 2455
CspCI CAANNNNNGTGG 2 cut(s) 1953, 1988
CviAII CATG 8 cut(s) 672, 1459, 1529, 1544, 1562, 2674, 2771, 2951
CviQI GTAC 7 cut(s) 305, 847, 937, 1283, 1995, 2151, 2455
DraI TTTAAA 1 cut(s) 997
DriI GACNNNNNGTC 1 cut(s) 970
Eam1104I CTCTTC 3 cut(s) 23, 317, 2803
Eam1105I GACNNNNNGTC 1 cut(s) 970
EarI CTCTTC 3 cut(s) 23, 317, 2803
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 2397
Eco24I GRGCYC 1 cut(s) 105
Eco32I GATATC 2 cut(s) 918, 1781
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 3 cut(s) 273, 2567, 3032
Eco72I CACGTG 1 cut(s) 1967
Eco81I CCTNAGG 1 cut(s) 483
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 2518
EcoRI GAATTC 1 cut(s) 1018
EcoRII CCWGG 2 cut(s) 114, 2511
EcoRV GATATC 2 cut(s) 918, 1781
EcoT14I CCWWGG 1 cut(s) 2397
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 2397
FaeI CATG 8 cut(s) 675, 1462, 1532, 1547, 1565, 2677, 2774, 2954
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 4 cut(s) 464, 2054, 2494, 2876
FatI CATG 8 cut(s) 671, 1458, 1528, 1543, 1561, 2673, 2770, 2950
FauI CCCGC 1 cut(s) 2044
FbaI TGATCA 1 cut(s) 1911
FblI GTMKAC 1 cut(s) 3250
Fnu4HI GCNGC 7 cut(s) 30, 260, 749, 2049, 2476, 2479, 2719
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 7 cut(s) 30, 260, 749, 2049, 2476, 2479, 2719
FspBI CTAG 5 cut(s) 50, 321, 1856, 1893, 2876
GlaI GCGC 5 cut(s) 258, 1311, 1498, 1727, 2565
GluI GCNGC 7 cut(s) 30, 260, 749, 2049, 2476, 2479, 2719
GsuI CTGGAG 2 cut(s) 137, 2534
HaeII RGCGCY 1 cut(s) 1500
HaeIII GGCC 2 cut(s) 2520, 3334
HapII CCGG 3 cut(s) 129, 2628, 3164
HhaI GCGC 5 cut(s) 259, 1312, 1499, 1728, 2566
Hin1I GRCGYC 1 cut(s) 2507
Hin1II CATG 8 cut(s) 675, 1462, 1532, 1547, 1565, 2677, 2774, 2954
Hin6I GCGC 5 cut(s) 257, 1310, 1497, 1726, 2564
HinP1I GCGC 5 cut(s) 257, 1310, 1497, 1726, 2564
HincII GTYRAC 3 cut(s) 727, 3106, 3251
HindII GTYRAC 3 cut(s) 727, 3106, 3251
HindIII AAGCTT 2 cut(s) 1535, 3053
HinfI GANTC 9 cut(s) 132, 640, 955, 1717, 1954, 2138, 2735, 3244, 3252
HpaII CCGG 3 cut(s) 129, 2628, 3164
HphI GGTGA 3 cut(s) 649, 1346, 2072
Hpy166II GTNNAC 7 cut(s) 567, 727, 939, 2063, 2218, 3106, 3251
Hpy8I GTNNAC 7 cut(s) 567, 727, 939, 2063, 2218, 3106, 3251
Hpy99I CGWCG 1 cut(s) 3252
HpyCH4III ACNGT 6 cut(s) 907, 936, 968, 1250, 2045, 2989
HpyCH4IV ACGT 6 cut(s) 24, 1966, 2507, 2870, 2889, 3185
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 3306
HpySE526I ACGT 6 cut(s) 24, 1966, 2507, 2870, 2889, 3185
Hsp92I GRCGYC 1 cut(s) 2507
Hsp92II CATG 8 cut(s) 675, 1462, 1532, 1547, 1565, 2677, 2774, 2954
HspAI GCGC 5 cut(s) 257, 1310, 1497, 1726, 2564
Ksp22I TGATCA 1 cut(s) 1911
LmnI GCTCC 8 cut(s) 59, 108, 117, 1221, 1978, 2753, 2980, 3005
Lsp1109I GCAGC 6 cut(s) 41, 271, 735, 2035, 2487, 2730
MaeI CTAG 5 cut(s) 50, 321, 1856, 1893, 2876
MaeII ACGT 6 cut(s) 24, 1966, 2507, 2870, 2889, 3185
MaeIII GTNAC 6 cut(s) 1701, 2835, 2866, 2871, 2989, 3186
MfeI CAATTG 3 cut(s) 1035, 2223, 3060
MflI RGATCY 5 cut(s) 370, 541, 1275, 1858, 2704
MhlI GDGCHC 2 cut(s) 105, 1218
MlyI GAGTC 3 cut(s) 1726, 1948, 3246
MmeI TCCRAC 6 cut(s) 519, 562, 675, 780, 1813, 2430
MroXI GAANNNNTTC 3 cut(s) 12, 1536, 3243
MslI CAYNNNNRTG 1 cut(s) 2577
MspA1I CMGCKG 1 cut(s) 2478
MspCI CTTAAG 4 cut(s) 943, 1918, 2075, 2417
MspI CCGG 3 cut(s) 129, 2628, 3164
MspR9I CCNGG 3 cut(s) 116, 2513, 2628
MunI CAATTG 3 cut(s) 1035, 2223, 3060
Mva1269I GAATGC 1 cut(s) 1965
MvaI CCWGG 2 cut(s) 116, 2513
MwoI GCNNNNNNNGC 2 cut(s) 109, 3306
NciI CCSGG 1 cut(s) 2628
NlaIII CATG 8 cut(s) 675, 1462, 1532, 1547, 1565, 2677, 2774, 2954
NlaIV GGNNCC 3 cut(s) 55, 543, 1514
NmuCI GTSAC 3 cut(s) 1701, 2835, 2871
NspI RCATGY 1 cut(s) 2677
NspV TTCGAA 1 cut(s) 1841
PaeI GCATGC 1 cut(s) 2677
PagI TCATGA 2 cut(s) 1458, 1528
PcsI WCGNNNNNNNCGW 1 cut(s) 1963
PctI GAATGC 1 cut(s) 1965
PdmI GAANNNNTTC 3 cut(s) 12, 1536, 3243
PfeI GAWTC 6 cut(s) 132, 640, 955, 2138, 2735, 3244
PflMI CCANNNNNTGG 1 cut(s) 2951
PfoI TCCNGGA 1 cut(s) 114
PkrI GCNGC 7 cut(s) 31, 261, 750, 2050, 2477, 2480, 2720
PleI GAGTC 3 cut(s) 1725, 1948, 3246
PmaCI CACGTG 1 cut(s) 1967
PmlI CACGTG 1 cut(s) 1967
PpsI GAGTC 3 cut(s) 1725, 1948, 3246
Ppu21I YACGTR 1 cut(s) 1967
PsiI TTATAA 2 cut(s) 449, 2243
Psp124BI GAGCTC 1 cut(s) 105
Psp1406I AACGTT 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 114, 2511
PspCI CACGTG 1 cut(s) 1967
PspGI CCWGG 2 cut(s) 114, 2511
PspN4I GGNNCC 3 cut(s) 55, 543, 1514
PspPI GGNCC 1 cut(s) 2518
PstI CTGCAG 1 cut(s) 3195
PsuI RGATCY 5 cut(s) 370, 541, 1275, 1858, 2704
RsaI GTAC 7 cut(s) 306, 848, 938, 1284, 1996, 2152, 2456
RsaNI GTAC 7 cut(s) 305, 847, 937, 1283, 1995, 2151, 2455
RseI CAYNNNNRTG 1 cut(s) 2577
SacI GAGCTC 1 cut(s) 105
SalI GTCGAC 1 cut(s) 3249
SatI GCNGC 7 cut(s) 30, 260, 749, 2049, 2476, 2479, 2719
Sau96I GGNCC 1 cut(s) 2518
ScaI AGTACT 1 cut(s) 848
SchI GAGTC 3 cut(s) 1726, 1948, 3246
ScrFI CCNGG 3 cut(s) 116, 2513, 2628
SduI GDGCHC 2 cut(s) 105, 1218
SfcI CTRYAG 2 cut(s) 1249, 3191
SfuI TTCGAA 1 cut(s) 1841
SmiMI CAYNNNNRTG 1 cut(s) 2577
SmlI CTYRAG 7 cut(s) 943, 1721, 1918, 2075, 2110, 2159, 2417
SmoI CTYRAG 7 cut(s) 943, 1721, 1918, 2075, 2110, 2159, 2417
SphI GCATGC 1 cut(s) 2677
SsiI CCGC 4 cut(s) 5, 2051, 2478, 3089
SspI AATATT 2 cut(s) 493, 2848
SspMI CTAG 5 cut(s) 50, 321, 1856, 1893, 2876
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 3 cut(s) 114, 2511, 2626
StyI CCWWGG 1 cut(s) 2397
TaaI ACNGT 6 cut(s) 907, 936, 968, 1250, 2045, 2989
TaiI ACGT 6 cut(s) 27, 1969, 2510, 2873, 2892, 3188
TaqI TCGA 6 cut(s) 195, 1574, 1841, 1957, 2723, 3250
TatI WGTACW 2 cut(s) 846, 2454
TauI GCSGC 1 cut(s) 2481
TfiI GAWTC 6 cut(s) 132, 640, 955, 2138, 2735, 3244
TscAI CASTG 3 cut(s) 1710, 2050, 3237
TseFI GTSAC 3 cut(s) 1701, 2835, 2871
TseI GCWGC 6 cut(s) 29, 259, 748, 2048, 2475, 2718
Tsp45I GTSAC 3 cut(s) 1701, 2835, 2871
TspGWI ACGGA 3 cut(s) 49, 2281, 2615
TspRI CASTG 3 cut(s) 1710, 2050, 3237
Van91I CCANNNNNTGG 1 cut(s) 2951
Vha464I CTTAAG 4 cut(s) 943, 1918, 2075, 2417
XceI RCATGY 1 cut(s) 2677
XmiI GTMKAC 1 cut(s) 3250
XmnI GAANNNNTTC 3 cut(s) 12, 1536, 3243
XspI CTAG 5 cut(s) 50, 321, 1856, 1893, 2876
ZraI GACGTC 1 cut(s) 2508
ZrmI AGTACT 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.