MD03G1280000.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000725
Physical Location & Seq
Reverse (-)
36145391 .. 36148884
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1280000.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGAAGTATTGCAACCCCGTTCAAATCTAAAGAAGTTGTTCGTGTATTATTATGGTGGTGTGAGGTTTGCGAGTTGGTTTTCTTCTCTCATAAATCTTGTTGATCTCAGATTGACTGGGTGTCATAGATGTCAACATCTTCCACCCTTGGATCATTTCCCATCCCTTAAGTCTCTTCAACTTGAAGGGTTTGAGAAATTGAAGTACATATCAGACAATACCAGCAGCAGTAATAGTATGAGTGATGAGATGATGACGTCCCTGGAGTTCCTCAGGGTTGTAAATTGCCCTGTTCTGAAGGGATGGTGGAGGGCGCACACTCATAACAGTGCTTCTTCTCCTTCATCAACGGAAAATCTGTCGTTGCCTTCTTTTCCCTGTCTTTCTACATTTTGGATCTGGGATTGTCCTAATCTAACTTCCATGCCTCTATATCCAAATGTGGAGACAATATCTCTCTATAAGAGCAGCTGGAAGGTTGTTGATTCCTTGTTTGGTAGAGGAGCATCTGATATTACACATGATGTTGGTGTTGTTGTCTCTGCCTCTTCTTCTTCCGCTCATCTCTCCAAATTAACACATCTGTCACTAGTTAAAATTGAGGATTTGGAATGTATAACGTCTCAAGGAATGGGCAACGTCCCATCACTCCAATCGCTTTACATTGCCAGTTGCCCAAATTTGGCATTACTACCATATCATGGGATGGGCAACCTCGCATCCTTTCGGGAGCTTACAGTTAGAAATTGCTCCAATTTGACATCAATGTTAGAAGGGATCGCCAATCTTACATCACTTCAGAAGCTTACAATTGGAGATTGCTCTAATCTGACATTACTGCCAGAAGTGGTCGTCAACCTCCCATCACTCCAATCGCTTACGATTGGATCTTTCCCAAAGTTGGTATCACTACTGGAAGAAATAAGCAATCTCACGTCACTGCAGCACCTTGCAAGAATTTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

323

Amino Acids

35.57

Weight (kDa)

6.54

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_R13L1-DRL21 PF25019 1 - 63 2.6e-17 R13L1/DRL21 LRRs
LRR_14 PF23598 222 - 319 7.6e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 701
AccBSI CCGCTC 1 cut(s) 560
AciI CCGC 1 cut(s) 558
AclWI GGATC 4 cut(s) 159, 404, 785, 895
AcsI RAATTY 2 cut(s) 679, 957
AcuI CTGAAG 2 cut(s) 317, 782
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 3 cut(s) 682, 701, 901
AflII CTTAAG 1 cut(s) 167
AgsI TTSAA 4 cut(s) 24, 179, 185, 202
AhdI GACNNNNNGTC 1 cut(s) 120
AhlI ACTAGT 1 cut(s) 589
AjiI CACGTC 1 cut(s) 936
AjnI CCWGG 1 cut(s) 261
AluBI AGCT 3 cut(s) 471, 733, 805
AluI AGCT 3 cut(s) 471, 733, 805
Alw26I GTCTC 4 cut(s) 177, 440, 544, 627
AlwI GGATC 4 cut(s) 159, 404, 785, 895
ApeKI GCWGC 3 cut(s) 225, 468, 943
ApoI RAATTY 2 cut(s) 679, 957
Asp700I GAANNNNTTC 1 cut(s) 38
AspLEI GCGC 1 cut(s) 316
AxyI CCTNAGG 1 cut(s) 272
BbvI GCAGC 3 cut(s) 237, 480, 955
BccI CCATC 5 cut(s) 169, 297, 652, 700, 871
BciT130I CCWGG 1 cut(s) 263
BcoDI GTCTC 4 cut(s) 177, 440, 544, 627
BcuI ACTAGT 1 cut(s) 589
BfaI CTAG 1 cut(s) 590
BfmI CTRYAG 1 cut(s) 941
BfrI CTTAAG 1 cut(s) 167
BisI GCNGC 3 cut(s) 226, 469, 944
BlsI GCNGC 3 cut(s) 227, 470, 945
Bme1390I CCNGG 1 cut(s) 263
BmeRI GACNNNNNGTC 1 cut(s) 120
BmgBI CACGTC 1 cut(s) 936
BmrFI CCNGG 1 cut(s) 263
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 2 cut(s) 515, 728
BmuI ACTGGG 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 609
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 147, 261
Bsc4I CCNNNNNNNGG 3 cut(s) 682, 701, 901
Bse1I ACTGG 3 cut(s) 121, 669, 918
Bse21I CCTNAGG 1 cut(s) 272
Bse3DI GCAATG 1 cut(s) 663
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 147, 261
BseGI GGATG 4 cut(s) 161, 308, 711, 719
BseLI CCNNNNNNNGG 3 cut(s) 682, 701, 901
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 2 cut(s) 121, 286
BseNI ACTGG 3 cut(s) 121, 669, 918
BseRI GAGGAG 1 cut(s) 516
BseXI GCAGC 3 cut(s) 237, 480, 955
BslFI GGGAC 2 cut(s) 244, 626
BslI CCNNNNNNNGG 3 cut(s) 682, 701, 901
BsmAI GTCTC 4 cut(s) 177, 440, 544, 627
BsmBI CGTCTC 1 cut(s) 627
BsmFI GGGAC 2 cut(s) 244, 626
Bsp143I GATC 5 cut(s) 103, 151, 396, 777, 887
BspACI CCGC 1 cut(s) 558
BspCNI CTCAG 2 cut(s) 120, 285
BspMAI CTGCAG 1 cut(s) 945
BspPI GGATC 4 cut(s) 159, 404, 785, 895
BspTI CTTAAG 1 cut(s) 167
BsrBI CCGCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 663
BsrI ACTGG 3 cut(s) 121, 669, 918
BssECI CCNNGG 2 cut(s) 147, 261
BssMI GATC 5 cut(s) 103, 151, 396, 777, 887
BssNI GRCGYC 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 329, 739
Bst6I CTCTTC 2 cut(s) 180, 553
BstACI GRCGYC 1 cut(s) 257
BstAFI CTTAAG 1 cut(s) 167
BstDEI CTNAG 2 cut(s) 107, 272
BstF5I GGATG 4 cut(s) 161, 308, 711, 719
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 5 cut(s) 106, 154, 399, 780, 890
BstMAI GTCTC 4 cut(s) 177, 440, 544, 627
BstMBI GATC 5 cut(s) 103, 151, 396, 777, 887
BstNI CCWGG 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 941
BstV1I GCAGC 3 cut(s) 237, 480, 955
BstX2I RGATCY 2 cut(s) 396, 887
BstYI RGATCY 2 cut(s) 396, 887
Bsu36I CCTNAGG 1 cut(s) 272
BtrI CACGTC 1 cut(s) 936
BtsCI GGATG 4 cut(s) 161, 308, 711, 719
BtsI GCAGTG 1 cut(s) 938
BtsIMutI CAGTG 2 cut(s) 334, 938
CfoI GCGC 1 cut(s) 316
Csp6I GTAC 1 cut(s) 205
CviAII CATG 3 cut(s) 424, 521, 701
CviJI RGCY 3 cut(s) 471, 733, 805
CviKI_1 RGCY 3 cut(s) 471, 733, 805
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 2 cut(s) 107, 272
DpnI GATC 5 cut(s) 105, 153, 398, 779, 889
DpnII GATC 5 cut(s) 103, 151, 396, 777, 887
DriI GACNNNNNGTC 1 cut(s) 120
Eam1104I CTCTTC 2 cut(s) 180, 553
Eam1105I GACNNNNNGTC 1 cut(s) 120
EarI CTCTTC 2 cut(s) 180, 553
Eco130I CCWWGG 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 317, 782
Eco81I CCTNAGG 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 627
FaeI CATG 3 cut(s) 427, 524, 704
FaqI GGGAC 2 cut(s) 244, 626
FatI CATG 3 cut(s) 423, 520, 700
Fnu4HI GCNGC 3 cut(s) 226, 469, 944
FokI GGATG 4 cut(s) 148, 315, 706, 718
Fsp4HI GCNGC 3 cut(s) 226, 469, 944
FspBI CTAG 1 cut(s) 590
GlaI GCGC 1 cut(s) 315
GluI GCNGC 3 cut(s) 226, 469, 944
GsuI CTGGAG 1 cut(s) 284
HhaI GCGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 3 cut(s) 427, 524, 704
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 2 cut(s) 134, 856
HindII GTYRAC 2 cut(s) 134, 856
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 1 cut(s) 485
Hpy166II GTNNAC 2 cut(s) 134, 856
Hpy188I TCNGA 6 cut(s) 110, 214, 297, 511, 801, 831
Hpy188III TCNNGA 1 cut(s) 728
Hpy8I GTNNAC 2 cut(s) 134, 856
HpyAV CCTTC 6 cut(s) 179, 292, 351, 378, 469, 767
HpyCH4III ACNGT 2 cut(s) 329, 739
HpyCH4IV ACGT 4 cut(s) 257, 620, 639, 935
HpyCH4V TGCA 3 cut(s) 13, 943, 953
HpyF3I CTNAG 2 cut(s) 107, 272
HpySE526I ACGT 4 cut(s) 257, 620, 639, 935
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 3 cut(s) 427, 524, 704
HspAI GCGC 1 cut(s) 314
Kzo9I GATC 5 cut(s) 103, 151, 396, 777, 887
LmnI GCTCC 3 cut(s) 503, 730, 755
Lsp1109I GCAGC 3 cut(s) 237, 480, 955
LweI GCATC 2 cut(s) 515, 728
MaeI CTAG 1 cut(s) 590
MaeII ACGT 4 cut(s) 257, 620, 639, 935
MaeIII GTNAC 2 cut(s) 585, 936
MalI GATC 5 cut(s) 105, 153, 398, 779, 889
MbiI CCGCTC 1 cut(s) 560
MboI GATC 5 cut(s) 103, 151, 396, 777, 887
MboII GAAGA 8 cut(s) 75, 131, 167, 327, 540, 543, 546, 929
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 2 cut(s) 396, 887
MluCI AATT 9 cut(s) 197, 283, 572, 597, 679, 745, 754, 810, 957
MnlI CCTC 9 cut(s) 57, 281, 303, 438, 494, 556, 595, 725, 869
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 3 cut(s) 168, 575, 594
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 471
MspCI CTTAAG 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 263
MunI CAATTG 1 cut(s) 810
MvaI CCWGG 1 cut(s) 263
NdeII GATC 5 cut(s) 103, 151, 396, 777, 887
NlaIII CATG 3 cut(s) 427, 524, 704
NmuCI GTSAC 2 cut(s) 585, 936
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 701
PkrI GCNGC 3 cut(s) 227, 470, 945
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PstI CTGCAG 1 cut(s) 945
PsuI RGATCY 2 cut(s) 396, 887
PvuII CAGCTG 1 cut(s) 471
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 327
SaqAI TTAA 3 cut(s) 168, 575, 594
SatI GCNGC 3 cut(s) 226, 469, 944
Sau3AI GATC 5 cut(s) 103, 151, 396, 777, 887
ScrFI CCNGG 1 cut(s) 263
SfaNI GCATC 2 cut(s) 515, 728
SfcI CTRYAG 1 cut(s) 941
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 2 cut(s) 167, 624
SmoI CTYRAG 2 cut(s) 167, 624
SpeI ACTAGT 1 cut(s) 589
Sse9I AATT 9 cut(s) 197, 283, 572, 597, 679, 745, 754, 810, 957
SsiI CCGC 1 cut(s) 558
SspMI CTAG 1 cut(s) 590
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 2 cut(s) 329, 739
TaiI ACGT 4 cut(s) 260, 623, 642, 938
TasI AATT 9 cut(s) 197, 283, 572, 597, 679, 745, 754, 810, 957
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 3 cut(s) 168, 575, 594
Tru9I TTAA 3 cut(s) 168, 575, 594
TscAI CASTG 2 cut(s) 334, 945
TseFI GTSAC 2 cut(s) 585, 936
TseI GCWGC 3 cut(s) 225, 468, 943
Tsp45I GTSAC 2 cut(s) 585, 936
TspDTI ATGAA 1 cut(s) 333
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 2 cut(s) 334, 945
Van91I CCANNNNNTGG 1 cut(s) 701
Vha464I CTTAAG 1 cut(s) 167
XapI RAATTY 2 cut(s) 679, 957
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 1 cut(s) 590
ZraI GACGTC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.