MD07G1016200.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000725
Physical Location & Seq
Forward (+)
1441276 .. 1442848
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1016200.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGAGGTTGTAAGAAAATGTCAGGGAGTTCCACTCGCTCTAAGGACGATAGGTGGGATGTTGCGCACCAAACATCATGAAATAGAATGGCTTAATTTCAAAGAAAGGAAACTTTCAAAAATAAGTCAAAAAGAAGATGATATTTTACCAACACTTAAACTAAGTTATGATGTGCTCCCATCACATTTGAAGCATTGTTTTGCTTATTGTAGCTTGTTCCCACCTGATTATGATATCTCTGTACCAAGATTGATTAGACTTTGGGTGGCCCAAGGGTTCGTTAAGTCATCCGACGAAATCGAGTGTTTGGAGGATGTTGCGTTTGAATATTATAGGGAATTGTTGTGCAGATCGTTTTTTCAAGAAGAAAAAACAAATGAGTTTGGTATAATAGAAAGTTGTAAAATGCACGATCTCATGAATGAACTTGCAATCTTAGTGTCGGGTGTCAGGAGCGTCGTAGTTGATCTGAACCGAAAGAACTTTCATGAAAAGCTTCTGCCAACTTCCTTGGTAAAAGCATGCAAGATACGAACTTATCTTTTTCTTTGCCAAGAGGTTTTGAAAGATTACGATGAGTCATTATGTGATTCATTTTGTACCACAATTGTTTCAAATTTTAAGTCATTACGGATGTTGAGTCTCAATACAATATATATTATAAGATTGCCTAATTGTTTTAAAAAAATGAAACATTTGAGATATCTTGATCTTAGTGGGAATCTCATGATGGAGAGACTTCCAGATTGGATAGTTGGACTTTCGAATTTGGAAACACTAGATCTCAGTCAGTGTCTTAGTCTTGTGGAATTGCCTAGAGACATTAAAAAAATGATCAACTTAAGGCATCTCATCTTGGAAGATTGTATTGGGTTGAGTGGAATGCCACGTGGAATTGGTGAATTGAAAGGTGTTCGTACATTGAATAGATTTGTTTTGAGTGAAAGCAATTGTTTGGGGAGGGGCGGTAGTGCTGGTCTTGCTGAGCTGGCCACCCTTAACGAATTAAGGGGAGACTTGGTTATTTTTGATAATTTGAGGCACGAGAAAGATGTGGTGTCAGAATCAAATGTTGGTACACCTCTCAAGGACAAACAGCATCTCCATTCGTTGGAATTGTGGTGGAAATTTGGAGAAGATGTAAAGGCAGTTGATGAGGTGGATATTATAAAGTCAATGGAAGTATTGCAACCCCATTCTAATCTAAAGCAGTTGTCCGTGTGGTATTATGGTGGTGTGAGGTTTGCGAGTTGGTTTTCTTCTCTCATTAATATTGTTAATCTCGCATTCTGGAATTGTAAGAGATGCCAACATCTTCCACCCTTGGATCATTTGCCTTCCCTTAAGAAACTTCAACTTGGATATTTGGAGAAGTTGGAGTACATATCAGAGAAAGAGAGCAGTAATAACACTCTGCCTAGTAGGTTGCCCTGTTCTGAAGGGATGGTGGAGGGCGCACACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

489

Amino Acids

56.06

Weight (kDa)

6.74

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 72 - 144 9.4e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 203 - 458 1.2e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 327 - 454 3.1e-29 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 662, 1169
Acc16I TGCGCA 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 1111
AciI CCGC 1 cut(s) 966
AclWI GGATC 1 cut(s) 1335
AcoI YGGCCR 1 cut(s) 990
AcsI RAATTY 3 cut(s) 616, 766, 1127
AcuI CTGAAG 1 cut(s) 1458
AcvI CACGTG 1 cut(s) 890
AfaI GTAC 5 cut(s) 243, 601, 919, 1078, 1382
AfiI CCNNNNNNNGG 1 cut(s) 1111
AflII CTTAAG 2 cut(s) 841, 1343
AjuI GAANNNNNNNTTGG 4 cut(s) 852, 884, 1056, 1088
AluBI AGCT 3 cut(s) 213, 496, 988
AluI AGCT 3 cut(s) 213, 496, 988
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 4 cut(s) 647, 730, 813, 1008
AlwI GGATC 1 cut(s) 1335
AoxI GGCC 2 cut(s) 267, 990
ApoI RAATTY 3 cut(s) 616, 766, 1127
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AseI ATTAAT 1 cut(s) 1269
Asp700I GAANNNNTTC 1 cut(s) 495
AspLEI GCGC 2 cut(s) 66, 1457
AspS9I GGNCC 1 cut(s) 268
AsuHPI GGTGA 1 cut(s) 911
AsuII TTCGAA 1 cut(s) 764
BalI TGGCCA 1 cut(s) 992
BarI GAAGNNNNNNTAC 2 cut(s) 1364, 1396
BauI CACGAG 1 cut(s) 1043
BbrPI CACGTG 1 cut(s) 890
Bbv12I GWGCWC 1 cut(s) 177
BccI CCATC 3 cut(s) 187, 724, 1438
BclI TGATCA 1 cut(s) 834
BcoDI GTCTC 4 cut(s) 647, 730, 813, 1008
BfaI CTAG 3 cut(s) 779, 816, 1419
BfrI CTTAAG 2 cut(s) 841, 1343
BglII AGATCT 1 cut(s) 781
BlpI GCTNAGC 1 cut(s) 984
BmgT120I GGNCC 1 cut(s) 268
BmsI GCATC 3 cut(s) 856, 1108, 1295
BplI GAGNNNNNCTC 2 cut(s) 18, 50
Bpu1102I GCTNAGC 1 cut(s) 984
Bpu14I TTCGAA 1 cut(s) 764
BpuEI CTTGAG 1 cut(s) 1070
BsaAI YACGTR 1 cut(s) 890
BsaJI CCNNGG 3 cut(s) 271, 510, 1323
BsaXI ACNNNNNCTCC 8 cut(s) 952, 982, 1005, 1035, 1086, 1116, 1125, 1155
Bsc4I CCNNNNNNNGG 1 cut(s) 1111
BseDI CCNNGG 3 cut(s) 271, 510, 1323
BseGI GGATG 5 cut(s) 63, 287, 319, 639, 1449
BseLI CCNNNNNNNGG 1 cut(s) 1111
BseMII CTCAG 2 cut(s) 799, 975
BsgI GTGCAG 1 cut(s) 367
BshFI GGCC 2 cut(s) 269, 992
BsiHKAI GWGCWC 1 cut(s) 177
BslI CCNNNNNNNGG 1 cut(s) 1111
BsmAI GTCTC 4 cut(s) 647, 730, 813, 1008
BsmI GAATGC 2 cut(s) 888, 1286
BsnI GGCC 2 cut(s) 269, 992
Bsp119I TTCGAA 1 cut(s) 764
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 7 cut(s) 350, 412, 466, 709, 781, 834, 1327
Bsp1720I GCTNAGC 1 cut(s) 984
BspACI CCGC 1 cut(s) 966
BspANI GGCC 2 cut(s) 269, 992
BspCNI CTCAG 2 cut(s) 798, 976
BspHI TCATGA 4 cut(s) 76, 417, 487, 726
BspPI GGATC 1 cut(s) 1335
BspT104I TTCGAA 1 cut(s) 764
BspTI CTTAAG 2 cut(s) 841, 1343
BssECI CCNNGG 3 cut(s) 271, 510, 1323
BssMI GATC 7 cut(s) 350, 412, 466, 709, 781, 834, 1327
BssSI CACGAG 1 cut(s) 1043
BssT1I CCWWGG 3 cut(s) 271, 510, 1323
Bst2BI CACGAG 1 cut(s) 1043
BstAFI CTTAAG 2 cut(s) 841, 1343
BstBAI YACGTR 1 cut(s) 890
BstBI TTCGAA 1 cut(s) 764
BstC8I GCNNGC 2 cut(s) 523, 990
BstDEI CTNAG 7 cut(s) 41, 161, 436, 713, 785, 797, 984
BstF5I GGATG 5 cut(s) 63, 287, 319, 639, 1449
BstHHI GCGC 2 cut(s) 66, 1457
BstKTI GATC 7 cut(s) 353, 415, 469, 712, 784, 837, 1330
BstMAI GTCTC 4 cut(s) 647, 730, 813, 1008
BstMBI GATC 7 cut(s) 350, 412, 466, 709, 781, 834, 1327
BstMWI GCNNNNNNNGC 2 cut(s) 980, 989
BstNSI RCATGY 1 cut(s) 525
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BsuRI GGCC 2 cut(s) 269, 992
BtsCI GGATG 5 cut(s) 63, 287, 319, 639, 1449
BtsIMutI CAGTG 1 cut(s) 797
Cac8I GCNNGC 2 cut(s) 523, 990
CciI TCATGA 4 cut(s) 76, 417, 487, 726
CfoI GCGC 2 cut(s) 66, 1457
Cfr13I GGNCC 1 cut(s) 268
CseI GACGC 1 cut(s) 445
Csp6I GTAC 5 cut(s) 242, 600, 918, 1077, 1381
CviAII CATG 5 cut(s) 77, 418, 488, 522, 727
CviJI RGCY 6 cut(s) 91, 213, 269, 496, 988, 992
CviKI_1 RGCY 6 cut(s) 91, 213, 269, 496, 988, 992
CviQI GTAC 5 cut(s) 242, 600, 918, 1077, 1381
DdeI CTNAG 7 cut(s) 41, 161, 436, 713, 785, 797, 984
DpnI GATC 7 cut(s) 352, 414, 468, 711, 783, 836, 1329
DpnII GATC 7 cut(s) 350, 412, 466, 709, 781, 834, 1327
DraI TTTAAA 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 990
Eco130I CCWWGG 3 cut(s) 271, 510, 1323
Eco32I GATATC 2 cut(s) 235, 704
Eco57I CTGAAG 1 cut(s) 1458
Eco72I CACGTG 1 cut(s) 890
EcoRV GATATC 2 cut(s) 235, 704
EcoT14I CCWWGG 3 cut(s) 271, 510, 1323
ErhI CCWWGG 3 cut(s) 271, 510, 1323
FaeI CATG 5 cut(s) 80, 421, 491, 525, 730
FatI CATG 5 cut(s) 76, 417, 487, 521, 726
FbaI TGATCA 1 cut(s) 834
FokI GGATG 5 cut(s) 70, 274, 326, 646, 1456
FspBI CTAG 3 cut(s) 779, 816, 1419
FspI TGCGCA 1 cut(s) 65
GlaI GCGC 2 cut(s) 65, 1456
HaeIII GGCC 2 cut(s) 269, 992
HgaI GACGC 1 cut(s) 445
HhaI GCGC 2 cut(s) 66, 1457
Hin1II CATG 5 cut(s) 80, 421, 491, 525, 730
Hin6I GCGC 2 cut(s) 64, 1455
HinP1I GCGC 2 cut(s) 64, 1455
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 5 cut(s) 578, 590, 640, 721, 1064
HphI GGTGA 1 cut(s) 911
Hpy166II GTNNAC 1 cut(s) 1079
Hpy188I TCNGA 5 cut(s) 292, 471, 1063, 1390, 1438
Hpy188III TCNNGA 9 cut(s) 77, 362, 418, 451, 488, 707, 727, 743, 1291
Hpy8I GTNNAC 1 cut(s) 1079
Hpy99I CGWCG 2 cut(s) 296, 461
HpyAV CCTTC 2 cut(s) 1347, 1433
HpyCH4IV ACGT 1 cut(s) 889
HpyCH4V TGCA 5 cut(s) 348, 409, 431, 525, 1189
HpyF10VI GCNNNNNNNGC 2 cut(s) 980, 989
HpyF3I CTNAG 7 cut(s) 41, 161, 436, 713, 785, 797, 984
HpySE526I ACGT 1 cut(s) 889
Hsp92II CATG 5 cut(s) 80, 421, 491, 525, 730
HspAI GCGC 2 cut(s) 64, 1455
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 7 cut(s) 350, 412, 466, 709, 781, 834, 1327
LmnI GCTCC 2 cut(s) 180, 453
LpnPI CCDG 8 cut(s) 8, 237, 436, 756, 960, 974, 1276, 1444
LweI GCATC 3 cut(s) 856, 1108, 1295
MaeI CTAG 3 cut(s) 779, 816, 1419
MaeII ACGT 1 cut(s) 889
MalI GATC 7 cut(s) 352, 414, 468, 711, 783, 836, 1329
MboI GATC 7 cut(s) 350, 412, 466, 709, 781, 834, 1327
MboII GAAGA 6 cut(s) 146, 377, 872, 1148, 1251, 1307
MfeI CAATTG 2 cut(s) 606, 949
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 1 cut(s) 177
MlsI TGGCCA 1 cut(s) 992
MluNI TGGCCA 1 cut(s) 992
MlyI GAGTC 2 cut(s) 587, 649
MmeI TCCRAC 4 cut(s) 315, 736, 1092, 1356
MnlI CCTC 8 cut(s) 304, 550, 954, 1032, 1092, 1150, 1233, 1444
Mox20I TGGCCA 1 cut(s) 992
MroXI GAANNNNTTC 1 cut(s) 495
MscI TGGCCA 1 cut(s) 992
Msp20I TGGCCA 1 cut(s) 992
MspCI CTTAAG 2 cut(s) 841, 1343
MunI CAATTG 2 cut(s) 606, 949
Mva1269I GAATGC 2 cut(s) 888, 1286
MwoI GCNNNNNNNGC 2 cut(s) 980, 989
NdeII GATC 7 cut(s) 350, 412, 466, 709, 781, 834, 1327
NlaIII CATG 5 cut(s) 80, 421, 491, 525, 730
NsbI TGCGCA 1 cut(s) 65
NspI RCATGY 1 cut(s) 525
NspV TTCGAA 1 cut(s) 764
PaeI GCATGC 1 cut(s) 525
PagI TCATGA 4 cut(s) 76, 417, 487, 726
PctI GAATGC 2 cut(s) 888, 1286
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 3 cut(s) 590, 721, 1064
PflMI CCANNNNNTGG 1 cut(s) 1111
PleI GAGTC 2 cut(s) 586, 648
PmaCI CACGTG 1 cut(s) 890
PmlI CACGTG 1 cut(s) 890
PpsI GAGTC 2 cut(s) 586, 648
Ppu21I YACGTR 1 cut(s) 890
PshBI ATTAAT 1 cut(s) 1269
PsiI TTATAA 2 cut(s) 662, 1169
PspCI CACGTG 1 cut(s) 890
PspPI GGNCC 1 cut(s) 268
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 5 cut(s) 243, 601, 919, 1078, 1382
RsaNI GTAC 5 cut(s) 242, 600, 918, 1077, 1381
Sau3AI GATC 7 cut(s) 350, 412, 466, 709, 781, 834, 1327
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 2 cut(s) 587, 649
SduI GDGCHC 1 cut(s) 177
SfaNI GCATC 3 cut(s) 856, 1108, 1295
SfuI TTCGAA 1 cut(s) 764
SmlI CTYRAG 3 cut(s) 841, 1085, 1343
SmoI CTYRAG 3 cut(s) 841, 1085, 1343
SphI GCATGC 1 cut(s) 525
SsiI CCGC 1 cut(s) 966
SspI AATATT 2 cut(s) 329, 1273
SspMI CTAG 3 cut(s) 779, 816, 1419
StyI CCWWGG 3 cut(s) 271, 510, 1323
TaiI ACGT 1 cut(s) 892
TaqI TCGA 2 cut(s) 300, 764
TatI WGTACW 1 cut(s) 1380
TfiI GAWTC 3 cut(s) 590, 721, 1064
TscAI CASTG 1 cut(s) 797
TspDTI ATGAA 7 cut(s) 93, 434, 438, 476, 504, 582, 704
TspGWI ACGGA 2 cut(s) 646, 1207
TspRI CASTG 1 cut(s) 797
Van91I CCANNNNNTGG 1 cut(s) 1111
Vha464I CTTAAG 2 cut(s) 841, 1343
VspI ATTAAT 1 cut(s) 1269
XapI RAATTY 3 cut(s) 616, 766, 1127
XceI RCATGY 1 cut(s) 525
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 3 cut(s) 779, 816, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.