MD07G1019600.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000725
Physical Location & Seq
Reverse (-)
1668893 .. 1672808
3916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1019600.v1.1.491

Sequence Viewer

Length: 1989 bp
ATGCTTGTGGAATTGCCTAGAGACATTAAAAAAATGATCAACTTAAGGCATCTCATCTTGGAATGCTGTTGGGGATTGACTGGAATGCCACGTGGAATTGGTGAATTGAATGGTGTTCGTACATTGAATAGATTTGTTTTGAGCGAAAGCAATTGTTTGGGGAGGGGCGGTAGTGCTGGTCTTGCTGAGCTGGGGGCCCTTAACGAATTAAGGGGAGAGTTGGAGATAAGGAATTTGAGGCATGTGGTGTCAGAATCAAATGTTGGTACACCTCTCAAGGACAAACAGCATCTCCATTCGTTGGAATTGTGGTGGAAAGAGGAAGAAGATGTAAAGGCCGTTGATGAGGAGGATATTATAAAGTCAATGGAAGTATTGCAACCCCATTCTAATCTAAAGCAGTTGTCCGTGTATAACTATAGGGGTGTGAGGTTTGCGAGTTGGTTTTCTTCTCTCATTAATATTGTTAATCTCAAATTGAGTAATTGTAAGAGATGCCAACATCTTCCACCCTTGGATCATTTGCCTTCCCTTAAGAAACTTGAACTTCGAAGTTTGGAGAAGTTGGAGTACATATCAGAGAAAGAGAGCAGTAATAGTATGAGTGATGAGATGATGAGGATCTCATTCTTTCCCTCCCTGGAAAATCTCTTTATATCTCAGTGCCCTGTTCTGAAGGGATGGTGGAGGGCCCATACTCATAACACTGCTTCTTCTTCATCAACGGAAAATTTGTCCTTGCCTTCTTTTCCCCGTCTTTCTCGATTGATTGTCAGGAATTGTCCTAATCTAACTTCCATGCCTCTGTATCCAAATGTGGAGAGCATAGAACTTAGGAATACCAGCTGGAAGGTTGTTGAATCCTTGTTTGTTAGAGGAGCATCTGATATTACACATGATGTTGGTGTTGATGTTTCTGCCTCTTCTTCTTCCCCTCATCTCTCCAAATTAACACATCTGTCACTCTGGGGAATTGAGGATTTGGAATTTATACCGTTGGAAGGGATGGGCAATCTGACGTCACTTCAGAAGCTTGTAATTGACCATTGCCCAAATTTGGCAGCACTACCGGAAGGGATCGCTAACCTCACATCACTCCAATCACTTTCCATTGGAAACTTCCCAAATTTGGCGGTACTACCAGAATGGATCGCGAACCTCACATCACTACAATCACTTTCAATTGAAAATTTCCCAAATCTGGCAGCATTACCAGAAGGAATCGTCTTCCTCACATCACTCCAATCGCTTAGGATTGAAGATTGCCCAACTTTGGCAGCACTACCAGAAGGGATCGCCAACCTCACTTCACTCCATTCGCTTTGTATTGAAGATTGCCCAAATTTGGCAGCACTACCAGAAGGGATCGCCAACCTCACATCACTCCAATCGCTTAGGATTGAAGATTGCCCAACTTTGGCAGCACTACCAGAAGGGATCGCCAACCTCACATCACTCCAATCGCTTAGGATTGAAGATTGCCCAACTTTGGCAGCACTACCAGAAGGGATCGCCAACCTCACATCACTCCAATCGCTTTCCATTGGAAATTTCCCAAATTGGGCAGCACTACCAGAAGGGATCGCCAACCTCACATCACTCCGATCGCTTTCCATTGGAAATTTCCCAAATTTGGCAGCACTACCAGAATGGATCGCCAACCTCACATCACTCCGATCGCTTTCCATTGGAAATTTCCCAAATTGGGCAGCACTACCAGATGGGATCGCCAACTTCACATCACTACAATCGCTTTACATTGAAAATTTCCCAAATTTGGCAGCACTACCAGAAGAAATAAGCAATCTCACGTCACTGCATTACCTTCAAATTACTCATTGCTCTAATTTGGCATCACTGCCAGAAGGGATTCGTGGACTCCCCTGTTTAAATACATTGTACATTTATGATTGCCCCAAGTTACTCCAAAGATACAAGCAAGAAAGAGGTGAGTACTGGCACAAGATGACATGCTATGTGGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

663

Amino Acids

73.26

Weight (kDa)

5.42

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 3 - 220 1.3e-09 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 61 - 185 5.8e-35 R13L1/DRL21 LRRs
LRR_14 PF23598 306 - 396 4.8e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 313 - 446 2.9e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 401 - 467 2.8e-09 Leucine-rich repeat region
LRR_14 PF23598 448 - 516 5.2e-11 Leucine-rich repeat region
LRR_14 PF23598 473 - 563 1.2e-12 Leucine-rich repeat region
LRR_14 PF23598 522 - 587 2.3e-07 Leucine-rich repeat region
LRR_14 PF23598 552 - 637 1.1e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 359
AatII GACGTC 1 cut(s) 1022
AccB7I CCANNNNNTGG 1 cut(s) 301
AccII CGCG 1 cut(s) 1154
AciI CCGC 2 cut(s) 168, 1133
AcuI CTGAAG 2 cut(s) 695, 1010
AcvI CACGTG 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 1019
AfaI GTAC 6 cut(s) 121, 268, 572, 1137, 1901, 1955
AflII CTTAAG 2 cut(s) 43, 533
AjiI CACGTC 1 cut(s) 1812
AjnI CCWGG 1 cut(s) 639
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 3 cut(s) 190, 846, 1033
AluI AGCT 3 cut(s) 190, 846, 1033
Alw26I GTCTC 1 cut(s) 15
AoxI GGCC 3 cut(s) 195, 336, 690
ApaI GGGCCC 2 cut(s) 199, 694
AseI ATTAAT 1 cut(s) 459
Asp700I GAANNNNTTC 1 cut(s) 1869
AspS9I GGNCC 4 cut(s) 195, 196, 690, 691
AsuHPI GGTGA 2 cut(s) 113, 1961
AsuII TTCGAA 1 cut(s) 550
BaeGI GKGCMC 3 cut(s) 199, 668, 694
BanII GRGCYC 2 cut(s) 199, 694
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
BbrPI CACGTG 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 1219
BccI CCATC 3 cut(s) 675, 1000, 1715
BceAI ACGGC 1 cut(s) 323
BciT130I CCWGG 1 cut(s) 641
BciVI GTATCC 1 cut(s) 819
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 418
BfrI CTTAAG 2 cut(s) 43, 533
BfuI GTATCC 1 cut(s) 819
BlpI GCTNAGC 1 cut(s) 186
BmcAI AGTACT 1 cut(s) 1955
Bme1390I CCNGG 1 cut(s) 641
BmgBI CACGTC 1 cut(s) 1812
BmgT120I GGNCC 4 cut(s) 195, 196, 690, 691
BmiI GGNNCC 3 cut(s) 196, 197, 692
BmrFI CCNGG 1 cut(s) 641
BmsI GCATC 5 cut(s) 58, 298, 485, 890, 1862
BpiI GAAGAC 1 cut(s) 1219
Bpu10I CCTNAGC 3 cut(s) 1250, 1394, 1466
Bpu1102I GCTNAGC 1 cut(s) 186
Bpu14I TTCGAA 1 cut(s) 550
BpuEI CTTGAG 1 cut(s) 260
BsaAI YACGTR 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 620
BsaHI GRCGYC 1 cut(s) 1019
BsaJI CCNNGG 2 cut(s) 513, 639
BsaWI WCCGGW 1 cut(s) 1069
BsaXI ACNNNNNCTCC 4 cut(s) 154, 184, 276, 306
Bse1I ACTGG 2 cut(s) 85, 1961
Bse3DI GCAATG 2 cut(s) 1045, 1837
Bse8I GATNNNNATC 1 cut(s) 620
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 2 cut(s) 513, 639
BseGI GGATG 2 cut(s) 686, 1011
BseJI GATNNNNATC 1 cut(s) 620
BseMI GCAATG 2 cut(s) 1045, 1837
BseMII CTCAG 2 cut(s) 177, 674
BseNI ACTGG 2 cut(s) 85, 1961
BseRI GAGGAG 2 cut(s) 362, 891
BseSI GKGCMC 3 cut(s) 199, 668, 694
BseYI CCCAGC 1 cut(s) 190
Bsh1236I CGCG 1 cut(s) 1154
Bsh1285I CGRYCG 2 cut(s) 1607, 1679
BshFI GGCC 3 cut(s) 197, 338, 692
BsiEI CGRYCG 2 cut(s) 1607, 1679
BsiSI CCGG 1 cut(s) 1070
BsmAI GTCTC 1 cut(s) 15
BsmI GAATGC 2 cut(s) 68, 90
BsnI GGCC 3 cut(s) 197, 338, 692
Bsp119I TTCGAA 1 cut(s) 550
Bsp120I GGGCCC 2 cut(s) 195, 690
Bsp1286I GDGCHC 3 cut(s) 199, 668, 694
Bsp1407I TGTACA 1 cut(s) 1899
Bsp1720I GCTNAGC 1 cut(s) 186
Bsp68I TCGCGA 1 cut(s) 1154
BspACI CCGC 2 cut(s) 168, 1133
BspANI GGCC 3 cut(s) 197, 338, 692
BspCNI CTCAG 2 cut(s) 178, 673
BspFNI CGCG 1 cut(s) 1154
BspLI GGNNCC 3 cut(s) 196, 197, 692
BspT104I TTCGAA 1 cut(s) 550
BspTI CTTAAG 2 cut(s) 43, 533
BsrDI GCAATG 2 cut(s) 1045, 1837
BsrGI TGTACA 1 cut(s) 1899
BsrI ACTGG 2 cut(s) 85, 1961
BssECI CCNNGG 2 cut(s) 513, 639
BssNI GRCGYC 1 cut(s) 1019
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 1 cut(s) 996
Bst6I CTCTTC 1 cut(s) 928
BstACI GRCGYC 1 cut(s) 1019
BstAFI CTTAAG 2 cut(s) 43, 533
BstAUI TGTACA 1 cut(s) 1899
BstBAI YACGTR 1 cut(s) 92
BstBI TTCGAA 1 cut(s) 550
BstDEI CTNAG 6 cut(s) 186, 660, 833, 1250, 1394, 1466
BstF5I GGATG 2 cut(s) 686, 1011
BstFNI CGCG 1 cut(s) 1154
BstMAI GTCTC 1 cut(s) 15
BstMCI CGRYCG 2 cut(s) 1607, 1679
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 641
BstNSI RCATGY 2 cut(s) 245, 1974
BstSCI CCNGG 1 cut(s) 639
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 3 cut(s) 199, 668, 694
BstUI CGCG 1 cut(s) 1154
BstV2I GAAGAC 1 cut(s) 1219
BstX2I RGATCY 1 cut(s) 621
BstYI RGATCY 1 cut(s) 621
BsuI GTATCC 1 cut(s) 819
BsuRI GGCC 3 cut(s) 197, 338, 692
BtrI CACGTC 1 cut(s) 1812
BtsCI GGATG 2 cut(s) 686, 1011
BtsI GCAGTG 3 cut(s) 705, 1814, 1856
BtsIMutI CAGTG 4 cut(s) 668, 705, 1814, 1856
BtuMI TCGCGA 1 cut(s) 1154
Cfr13I GGNCC 4 cut(s) 195, 196, 690, 691
Csp6I GTAC 6 cut(s) 120, 267, 571, 1136, 1900, 1954
CviAII CATG 4 cut(s) 242, 799, 896, 1971
CviJI RGCY 6 cut(s) 190, 197, 338, 692, 846, 1033
CviKI_1 RGCY 6 cut(s) 190, 197, 338, 692, 846, 1033
CviQI GTAC 6 cut(s) 120, 267, 571, 1136, 1900, 1954
DdeI CTNAG 6 cut(s) 186, 660, 833, 1250, 1394, 1466
DraI TTTAAA 1 cut(s) 1890
Eam1104I CTCTTC 1 cut(s) 928
EarI CTCTTC 1 cut(s) 928
Eco130I CCWWGG 1 cut(s) 513
Eco24I GRGCYC 2 cut(s) 199, 694
Eco57I CTGAAG 2 cut(s) 695, 1010
Eco72I CACGTG 1 cut(s) 92
EcoO109I RGGNCCY 3 cut(s) 195, 196, 690
EcoRII CCWGG 1 cut(s) 639
EcoT14I CCWWGG 1 cut(s) 513
EcoT38I GRGCYC 2 cut(s) 199, 694
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 4 cut(s) 245, 802, 899, 1974
FatI CATG 4 cut(s) 241, 798, 895, 1970
FbaI TGATCA 1 cut(s) 36
FokI GGATG 2 cut(s) 693, 1018
FriOI GRGCYC 2 cut(s) 199, 694
FspBI CTAG 1 cut(s) 18
GsaI CCCAGC 1 cut(s) 194
HaeIII GGCC 3 cut(s) 197, 338, 692
HapII CCGG 1 cut(s) 1070
Hin1I GRCGYC 1 cut(s) 1019
Hin1II CATG 4 cut(s) 245, 802, 899, 1974
HindIII AAGCTT 1 cut(s) 1031
HinfI GANTC 5 cut(s) 254, 860, 1221, 1870, 1878
HpaII CCGG 1 cut(s) 1070
HphI GGTGA 2 cut(s) 113, 1961
Hpy166II GTNNAC 2 cut(s) 269, 1877
Hpy188I TCNGA 8 cut(s) 253, 580, 675, 886, 1017, 1029, 1604, 1676
Hpy188III TCNNGA 3 cut(s) 762, 775, 1153
Hpy8I GTNNAC 2 cut(s) 269, 1877
HpyCH4III ACNGT 1 cut(s) 996
HpyCH4IV ACGT 3 cut(s) 91, 1019, 1811
HpyCH4V TGCA 2 cut(s) 379, 1819
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 6 cut(s) 186, 660, 833, 1250, 1394, 1466
HpySE526I ACGT 3 cut(s) 91, 1019, 1811
Hsp92I GRCGYC 1 cut(s) 1019
Hsp92II CATG 4 cut(s) 245, 802, 899, 1974
Ksp22I TGATCA 1 cut(s) 36
LmnI GCTCC 1 cut(s) 878
LweI GCATC 5 cut(s) 58, 298, 485, 890, 1862
MaeI CTAG 1 cut(s) 18
MaeII ACGT 3 cut(s) 91, 1019, 1811
MaeIII GTNAC 4 cut(s) 960, 1020, 1812, 1920
MfeI CAATTG 2 cut(s) 151, 1182
MflI RGATCY 1 cut(s) 621
MhlI GDGCHC 3 cut(s) 199, 668, 694
MlyI GAGTC 1 cut(s) 1872
MmeI TCCRAC 4 cut(s) 201, 282, 546, 978
MroXI GAANNNNTTC 1 cut(s) 1869
MseI TTAA 9 cut(s) 27, 44, 201, 209, 459, 468, 534, 950, 1889
MspA1I CMGCKG 1 cut(s) 846
MspCI CTTAAG 2 cut(s) 43, 533
MspI CCGG 1 cut(s) 1070
MspR9I CCNGG 1 cut(s) 641
MunI CAATTG 2 cut(s) 151, 1182
Mva1269I GAATGC 2 cut(s) 68, 90
MvaI CCWGG 1 cut(s) 641
MvnI CGCG 1 cut(s) 1154
MwoI GCNNNNNNNGC 1 cut(s) 182
NlaIII CATG 4 cut(s) 245, 802, 899, 1974
NlaIV GGNNCC 3 cut(s) 196, 197, 692
NmuCI GTSAC 3 cut(s) 960, 1020, 1812
NruI TCGCGA 1 cut(s) 1154
NspI RCATGY 2 cut(s) 245, 1974
NspV TTCGAA 1 cut(s) 550
PctI GAATGC 2 cut(s) 68, 90
PdmI GAANNNNTTC 1 cut(s) 1869
PfeI GAWTC 4 cut(s) 254, 860, 1221, 1870
PflMI CCANNNNNTGG 1 cut(s) 301
Ple19I CGATCG 2 cut(s) 1607, 1679
PleI GAGTC 1 cut(s) 1872
PmaCI CACGTG 1 cut(s) 92
PmlI CACGTG 1 cut(s) 92
PpsI GAGTC 1 cut(s) 1872
Ppu21I YACGTR 1 cut(s) 92
PshBI ATTAAT 1 cut(s) 459
PsiI TTATAA 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 639
PspCI CACGTG 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 639
PspN4I GGNNCC 3 cut(s) 196, 197, 692
PspOMI GGGCCC 2 cut(s) 195, 690
PspPI GGNCC 4 cut(s) 195, 196, 690, 691
PsuI RGATCY 1 cut(s) 621
PvuI CGATCG 2 cut(s) 1607, 1679
PvuII CAGCTG 1 cut(s) 846
RruI TCGCGA 1 cut(s) 1154
RsaI GTAC 6 cut(s) 121, 268, 572, 1137, 1901, 1955
RsaNI GTAC 6 cut(s) 120, 267, 571, 1136, 1900, 1954
SaqAI TTAA 9 cut(s) 27, 44, 201, 209, 459, 468, 534, 950, 1889
Sau96I GGNCC 4 cut(s) 195, 196, 690, 691
ScaI AGTACT 1 cut(s) 1955
SchI GAGTC 1 cut(s) 1872
ScrFI CCNGG 1 cut(s) 641
SduI GDGCHC 3 cut(s) 199, 668, 694
SfaNI GCATC 5 cut(s) 58, 298, 485, 890, 1862
SfcI CTRYAG 1 cut(s) 418
SfuI TTCGAA 1 cut(s) 550
SmlI CTYRAG 3 cut(s) 43, 275, 533
SmoI CTYRAG 3 cut(s) 43, 275, 533
SsiI CCGC 2 cut(s) 168, 1133
SspI AATATT 1 cut(s) 463
SspMI CTAG 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 639
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 1 cut(s) 996
TaiI ACGT 3 cut(s) 94, 1022, 1814
TaqI TCGA 2 cut(s) 550, 763
TatI WGTACW 3 cut(s) 570, 1899, 1953
TfiI GAWTC 4 cut(s) 254, 860, 1221, 1870
Tru1I TTAA 9 cut(s) 27, 44, 201, 209, 459, 468, 534, 950, 1889
Tru9I TTAA 9 cut(s) 27, 44, 201, 209, 459, 468, 534, 950, 1889
TscAI CASTG 4 cut(s) 668, 712, 1821, 1863
TseFI GTSAC 3 cut(s) 960, 1020, 1812
Tsp45I GTSAC 3 cut(s) 960, 1020, 1812
TspDTI ATGAA 1 cut(s) 708
TspGWI ACGGA 2 cut(s) 397, 740
TspRI CASTG 4 cut(s) 668, 712, 1821, 1863
Van91I CCANNNNNTGG 1 cut(s) 301
Vha464I CTTAAG 2 cut(s) 43, 533
VspI ATTAAT 1 cut(s) 459
XceI RCATGY 2 cut(s) 245, 1974
XmnI GAANNNNTTC 1 cut(s) 1869
XspI CTAG 1 cut(s) 18
ZraI GACGTC 1 cut(s) 1020
ZrmI AGTACT 1 cut(s) 1955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.