pycom07g01160

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000725
Physical Location & Seq
Forward (+)
922093 .. 923217
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01160.1

Sequence Viewer

Length: 1125 bp
ATGCCACGTGGAATTGGTGAATTGAATGGTGTTCGTACATTGAGTAGATTTGTTTTGAGCGAAAGCAATTGTTTGGGGAGGGGAGGTAGTGCTGGTCTTGCCGAGCTGGGGACCCTTAAGGAATTAAGGGGAGACTTAGTCATTGGCAATTTGAGGCATGTGGTGTCAGAATCAAATGTTGGTACACCTCTCAAGGACAAACAGCATCTTTGTTCGTTGCAATTGTGGTGGATACTTCGAGAAGATATAAGCGCGGTTGATGAGGAGGATATTATAAAGTCAATGGAAGTATTGCAACCCCATTCTAATCTAAAGCAGTTGCAAGTGTGGTATTATGGTGGTGTGAGGTTTGCGAGTTGGTTTTCTTCTCTCATAAATATTGTTAATCTCGAATTGTCGTTTTTTAAGAGATGCCAACGTCTTCCGCCCTTGGATCGTTTGCCTTCCCTTAAGTCTCTTACAATGGTTGGGTTGCAGAAGTTGGAGTACATATCAGAGAATGAGAGCAGTAATAGTATGAGTGATGAGATGATGAGGATCTCATTCTTTCCCTCCCTGGAAAAGCTCTCCATACAAGATTGCCCTGTTCTGAAGGGATGGTGGAGGGCGCACGCTTGTAACAGTGACGATTCTTCTTCATCAACGGAAAATCTGTCGTTGCCTTCTTTTCCTCGTCTTTCTACATTATCTATTGATGAGTGTCCTAATCTAACTTCCATGCCTCTGTATCCAAATGTGGAGAAAATACATCTTGAGAAGAGCAGCTGGAAGGTTGTTGATTCCTTGTTTGTTAGAGGAGCATCTGATATTACACTTGATGTTGGTGTTGATGTTGATGTTTCTGCCTCTTCTTCTTCCCCTCATCTCTCCAAATTAACACATCTGTCACTCCATGCCATTGAGGATTTGGCATCACTGCCAGAAGAAATAAGCAATCTCACGTCATTGCAACACCTTGAATTTAGTAATTGCTCTAATTTGGCATCACTGCCAGAAGAAATAAGCAATCTCACATCACTTCAGGAGCTTGCAATTAGTAATTGCTCTAATTTGTCATCACTGCCAGAAGGGATTCGTGGACTCCCCTGTTTAAATAGATTGAAAATTTGGCGTGCCCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

375

Amino Acids

41.51

Weight (kDa)

5.36

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 1 - 194 6.3e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 33 - 156 9.8e-30 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 217 - 348 3.7e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 282 - 371 1.5e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g16700
malus_domestica MD02G1307600.v1.1 MD03G1278800.v1.1 MD03G1279100.v1.1 MD03G1279600.v1.1 MD03G1280000.v1.1 MD03G1280200.v1.1 MD07G1008400.v1.1 MD07G1014900.v1.1 MD07G1015700.v1.1 MD07G1016100.v1.1 MD07G1016200.v1.1 MD07G1016500.v1.1 MD07G1016600.v1.1 MD07G1016700.v1.1 MD07G1017400.v1.1 MD07G1019600.v1.1 MD07G1019800.v1.1 MD07G1023700.v1.1 MD07G1024100.v1.1 MD07G1024300.v1.1 MD07G1024400.v1.1 MD14G1086900.v1.1 MD14G1087000.v1.1 MD16G1179200.v1.1 MD17G1030800.v1.1
prunus_persica Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011000_v2.0.a1 Prupe.2G011400_v2.0.a1 Prupe.2G011500_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013800_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G013900_v2.0.a1 Prupe.2G014000_v2.0.a1 Prupe.2G014100_v2.0.a1
pyrus_communis pycom02g25880 pycom07g00690 pycom07g00720 pycom07g01130 pycom07g01140 pycom07g01150 pycom07g01160 pycom07g01260 pycom07g01280 pycom07g01300 pycom07g01320 pycom07g01550 pycom07g01790 pycom07g01810 pycom07g01840 pycom07g01850 pycom07g01870 pycom07g01880 pycom07g01890 pycom13g15290 pycom16g15040
rosa_chinensis RchiOBHm_Chr4g0420141
rosa_multiflora Rmu_sc0001252.1_g000002
rosa_roxburghii Rroxscaffold_1G00012760
rosa_rugosa Rorug04G0162600
rosa_samantha Rh4AG223400 Rh4BG225900 Rh4CG236600 Rh4DG222200
rosa_wichuraiana Rw4G019230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
AccII CGCG 1 cut(s) 254
AciI CCGC 2 cut(s) 254, 425
AclWI GGATC 2 cut(s) 441, 545
AcsI RAATTY 2 cut(s) 959, 1104
AcuI CTGAAG 2 cut(s) 611, 1004
AcvI CACGTG 1 cut(s) 8
AfaI GTAC 3 cut(s) 37, 184, 488
AfiI CCNNNNNNNGG 1 cut(s) 108
AflII CTTAAG 2 cut(s) 116, 449
AgsI TTSAA 3 cut(s) 25, 959, 1102
AjiI CACGTC 1 cut(s) 942
AjnI CCWGG 1 cut(s) 555
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 4 cut(s) 106, 565, 765, 1027
AluI AGCT 4 cut(s) 106, 565, 765, 1027
Alw26I GTCTC 2 cut(s) 126, 459
AlwI GGATC 2 cut(s) 441, 545
ApeKI GCWGC 1 cut(s) 762
ApoI RAATTY 2 cut(s) 959, 1104
Asp700I GAANNNNTTC 1 cut(s) 1071
AspLEI GCGC 2 cut(s) 254, 610
AspS9I GGNCC 1 cut(s) 111
AsuHPI GGTGA 1 cut(s) 29
AvaII GGWCC 1 cut(s) 111
BaeGI GKGCMC 1 cut(s) 1117
BarI GAAGNNNNNNTAC 2 cut(s) 470, 502
BbrPI CACGTG 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 413
BbvI GCAGC 1 cut(s) 774
BccI CCATC 1 cut(s) 591
BciT130I CCWGG 1 cut(s) 557
BciVI GTATCC 2 cut(s) 225, 738
BcoDI GTCTC 2 cut(s) 126, 459
BfrI CTTAAG 2 cut(s) 116, 449
BfuI GTATCC 2 cut(s) 225, 738
BisI GCNGC 1 cut(s) 763
BlsI GCNGC 1 cut(s) 764
Bme1390I CCNGG 1 cut(s) 557
Bme18I GGWCC 1 cut(s) 111
BmgBI CACGTC 1 cut(s) 942
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 2 cut(s) 112, 113
BmrFI CCNGG 1 cut(s) 557
BmsI GCATC 5 cut(s) 214, 401, 809, 920, 992
BpiI GAAGAC 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 176, 773
BsaAI YACGTR 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 536
BsaJI CCNNGG 2 cut(s) 429, 555
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 123, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 944
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 2 cut(s) 429, 555
BseGI GGATG 1 cut(s) 602
BseJI GATNNNNATC 1 cut(s) 536
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMI GCAATG 1 cut(s) 944
BseRI GAGGAG 2 cut(s) 278, 810
BseSI GKGCMC 1 cut(s) 1117
BseXI GCAGC 1 cut(s) 774
BseYI CCCAGC 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 126, 459
BsmFI GGGAC 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 1117
Bsp143I GATC 2 cut(s) 433, 537
BspACI CCGC 2 cut(s) 254, 425
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 2 cut(s) 112, 113
BspPI GGATC 2 cut(s) 441, 545
BspQI GCTCTTC 1 cut(s) 752
BspTI CTTAAG 2 cut(s) 116, 449
BsrDI GCAATG 1 cut(s) 944
BssECI CCNNGG 2 cut(s) 429, 555
BssMI GATC 2 cut(s) 433, 537
BssT1I CCWWGG 1 cut(s) 429
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 1 cut(s) 623
Bst6I CTCTTC 2 cut(s) 752, 853
BstAFI CTTAAG 2 cut(s) 116, 449
BstBAI YACGTR 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 612, 1029, 1113
BstDEI CTNAG 1 cut(s) 136
BstF5I GGATG 1 cut(s) 602
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 2 cut(s) 254, 610
BstKTI GATC 2 cut(s) 436, 540
BstMAI GTCTC 2 cut(s) 126, 459
BstMBI GATC 2 cut(s) 433, 537
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 555
BstSLI GKGCMC 1 cut(s) 1117
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 774
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 1 cut(s) 537
BstYI RGATCY 1 cut(s) 537
BsuI GTATCC 2 cut(s) 225, 738
BtrI CACGTC 1 cut(s) 942
BtsCI GGATG 1 cut(s) 602
BtsI GCAGTG 3 cut(s) 914, 986, 1058
BtsIMutI CAGTG 4 cut(s) 628, 914, 986, 1058
Cac8I GCNNGC 3 cut(s) 612, 1029, 1113
CfoI GCGC 2 cut(s) 254, 610
Cfr13I GGNCC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 36, 183, 487
CspCI CAANNNNNGTGG 2 cut(s) 209, 244
CviAII CATG 4 cut(s) 158, 718, 893, 1119
CviJI RGCY 4 cut(s) 106, 565, 765, 1027
CviKI_1 RGCY 4 cut(s) 106, 565, 765, 1027
CviQI GTAC 3 cut(s) 36, 183, 487
DdeI CTNAG 1 cut(s) 136
DpnI GATC 2 cut(s) 435, 539
DpnII GATC 2 cut(s) 433, 537
DraI TTTAAA 1 cut(s) 1092
Eam1104I CTCTTC 2 cut(s) 752, 853
EarI CTCTTC 2 cut(s) 752, 853
EciI GGCGGA 1 cut(s) 414
Eco130I CCWWGG 1 cut(s) 429
Eco47I GGWCC 1 cut(s) 111
Eco57I CTGAAG 2 cut(s) 611, 1004
Eco72I CACGTG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 4 cut(s) 161, 721, 896, 1122
FaqI GGGAC 1 cut(s) 124
FatI CATG 4 cut(s) 157, 717, 892, 1118
Fnu4HI GCNGC 1 cut(s) 763
FokI GGATG 1 cut(s) 609
Fsp4HI GCNGC 1 cut(s) 763
GlaI GCGC 2 cut(s) 253, 609
GluI GCNGC 1 cut(s) 763
GsaI CCCAGC 1 cut(s) 110
HhaI GCGC 2 cut(s) 254, 610
Hin1II CATG 4 cut(s) 161, 721, 896, 1122
Hin6I GCGC 2 cut(s) 252, 608
HinP1I GCGC 2 cut(s) 252, 608
HinfI GANTC 5 cut(s) 170, 629, 779, 1072, 1080
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 2 cut(s) 185, 1079
Hpy188I TCNGA 4 cut(s) 169, 496, 591, 805
Hpy188III TCNNGA 4 cut(s) 239, 389, 752, 1022
Hpy8I GTNNAC 2 cut(s) 185, 1079
HpyAV CCTTC 5 cut(s) 453, 586, 672, 763, 1061
HpyCH4III ACNGT 1 cut(s) 623
HpyCH4IV ACGT 3 cut(s) 7, 418, 941
HpyCH4V TGCA 6 cut(s) 220, 295, 322, 475, 949, 1031
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 1 cut(s) 136
HpySE526I ACGT 3 cut(s) 7, 418, 941
Hsp92II CATG 4 cut(s) 161, 721, 896, 1122
HspAI GCGC 2 cut(s) 252, 608
KflI GGGWCCC 1 cut(s) 111
Kzo9I GATC 2 cut(s) 433, 537
LguI GCTCTTC 1 cut(s) 752
LmnI GCTCC 2 cut(s) 797, 1024
Lsp1109I GCAGC 1 cut(s) 774
LweI GCATC 5 cut(s) 214, 401, 809, 920, 992
MaeII ACGT 3 cut(s) 7, 418, 941
MaeIII GTNAC 3 cut(s) 617, 623, 885
MalI GATC 2 cut(s) 435, 539
MboI GATC 2 cut(s) 433, 537
MfeI CAATTG 2 cut(s) 67, 221
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 1 cut(s) 1117
MlyI GAGTC 1 cut(s) 1074
MmeI TCCRAC 1 cut(s) 462
MroXI GAANNNNTTC 1 cut(s) 1071
MseI TTAA 8 cut(s) 117, 125, 384, 405, 450, 875, 1091, 1123
MspA1I CMGCKG 1 cut(s) 765
MspCI CTTAAG 2 cut(s) 116, 449
MspR9I CCNGG 1 cut(s) 557
MunI CAATTG 2 cut(s) 67, 221
MvaI CCWGG 1 cut(s) 557
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeII GATC 2 cut(s) 433, 537
NlaIII CATG 4 cut(s) 161, 721, 896, 1122
NlaIV GGNNCC 2 cut(s) 112, 113
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 2 cut(s) 623, 885
NspI RCATGY 1 cut(s) 161
PciSI GCTCTTC 1 cut(s) 752
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 4 cut(s) 170, 629, 779, 1072
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 1 cut(s) 764
PleI GAGTC 1 cut(s) 1074
PmaCI CACGTG 1 cut(s) 8
PmlI CACGTG 1 cut(s) 8
PpsI GAGTC 1 cut(s) 1074
Ppu21I YACGTR 1 cut(s) 8
PpuMI RGGWCCY 1 cut(s) 111
PsiI TTATAA 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 555
PspCI CACGTG 1 cut(s) 8
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 112, 113
PspPI GGNCC 1 cut(s) 111
PspPPI RGGWCCY 1 cut(s) 111
PsuI RGATCY 1 cut(s) 537
PsyI GACNNNGTC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 765
RsaI GTAC 3 cut(s) 37, 184, 488
RsaNI GTAC 3 cut(s) 36, 183, 487
SapI GCTCTTC 1 cut(s) 752
SaqAI TTAA 8 cut(s) 117, 125, 384, 405, 450, 875, 1091, 1123
SatI GCNGC 1 cut(s) 763
Sau3AI GATC 2 cut(s) 433, 537
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 1 cut(s) 1074
ScrFI CCNGG 1 cut(s) 557
SduI GDGCHC 1 cut(s) 1117
SfaNI GCATC 5 cut(s) 214, 401, 809, 920, 992
SinI GGWCC 1 cut(s) 111
SmlI CTYRAG 4 cut(s) 116, 191, 449, 752
SmoI CTYRAG 4 cut(s) 116, 191, 449, 752
SsiI CCGC 2 cut(s) 254, 425
SspI AATATT 1 cut(s) 379
StyD4I CCNGG 1 cut(s) 555
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 1 cut(s) 623
TaiI ACGT 3 cut(s) 10, 421, 944
TaqI TCGA 2 cut(s) 238, 390
TatI WGTACW 1 cut(s) 486
TfiI GAWTC 4 cut(s) 170, 629, 779, 1072
Tru1I TTAA 8 cut(s) 117, 125, 384, 405, 450, 875, 1091, 1123
Tru9I TTAA 8 cut(s) 117, 125, 384, 405, 450, 875, 1091, 1123
TscAI CASTG 4 cut(s) 628, 921, 993, 1065
TseFI GTSAC 2 cut(s) 623, 885
TseI GCWGC 1 cut(s) 762
Tsp45I GTSAC 2 cut(s) 623, 885
TspDTI ATGAA 1 cut(s) 627
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 4 cut(s) 628, 921, 993, 1065
Tth111I GACNNNGTC 1 cut(s) 137
Vha464I CTTAAG 2 cut(s) 116, 449
VpaK11BI GGWCC 1 cut(s) 111
XapI RAATTY 2 cut(s) 959, 1104
XceI RCATGY 1 cut(s) 161
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XmnI GAANNNNTTC 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.