FvH4_4g22070

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002269
Physical Location & Seq
Reverse (-)
25014200 .. 25016670
2471 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22070.t1

Sequence Viewer

Length: 1482 bp
ATGGCTGGCACAGGTTGCATTGTTCAAATTAATGAAGATGTCAAGAACATGGAAGGTCTTTCAACAGAAAAGGATCATTGGATTGTTCAAATTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAAAAGGATCATTGGAATAAGGGGTCGATCTACAAACTACCAGCCAGCCTCACAGACACAAATAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGGCCTTACCATTACGATCCTTCACATCCAATGGAGGCGCACAAGCACCGAGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGTCTGTTGAGTTGTTTGTAGATGCATTGGCGGAAGTGGAGAATGATTTGAAGGATTCATATACTCTTCTTCATTCTGTGCCAAAAGAAGTTACAGACATATTCCTACAAATGATGATTTTAGATGGCTGTTTCATGCTGGAAATCATGAGGACCGCTGCTCATGTACTGGATGATTATGCTCCTAATGATCCCATCTTCAGCGATCACGGTAGGGTGCATGTCGTGCCATTTATCAAGCGCGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTCGCAGTTGAACATGATAAAGCTAAGGATGCAGGGTTTGTGACCAAGCTCATAGTCAAGTTCCTTTTCTCCCGCAATGTTTCTGTAAACATGGGCAAATGTGTGCATGTGTTGGACTTGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCCCAAATATATTCGACACAAAATACGGTCCATGGAACAAGAAGAAGAGATCATATGGTCCGCAACAGAGCTCAACGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTTGCCCGGGGATTGTTGAGGCTCCCTGTCGTTGTTGTGGATGACACCACCGAGTCATTGTTCTTGAATCTGATGGCATTTGAGAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCTAGGGATGTCGCCCTCTTACACTCGAAAGGGATCATCCTTAATGGTCTAGGGAGTGACAAAGCAGTGGCTAACCTGTTCAATTCACTCTCTAAGGATGTGATACAGGACCCGGATAGCAGCCTCCAAATTGTGCATAAAAGGGTGTATCACTACTGCAAAAAGCCATGGAATGAGTGGCGCGCCAATCTTATTCACACTTACTTCAGAAATCCATGGGCTATTATTTCTTTCATTGCGGCTGTCTTCCTCTTTGTGTTCACTATAGGTCAGACGGGGTACTCCATATATCCCTATTACTATCCACGAGGTGATTCTTCTCCTTCCGAAGTCATTGTGGTTCCGGTGCCTCCTCCTCCACACCATTCATCCTCCGACTCCACCTCCCCATCAGCACTTGTCATTTCTTTCGTTCTTGCTTCCATTGTATGCATGTTTACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

494

Amino Acids

55.95

Weight (kDa)

6.26

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 53 - 431 1.3e-116 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1381
AccII CGCG 2 cut(s) 549, 1218
AciI CCGC 5 cut(s) 338, 462, 673, 813, 1274
AclWI GGATC 6 cut(s) 81, 144, 236, 491, 746, 1076
AcuI CTGAAG 3 cut(s) 490, 722, 1225
AdeI CACNNNGTG 1 cut(s) 1346
AfaI GTAC 3 cut(s) 474, 722, 1316
AfeI AGCGCT 1 cut(s) 279
AflIII ACRYGT 1 cut(s) 552
AluBI AGCT 4 cut(s) 598, 623, 649, 823
AluI AGCT 4 cut(s) 598, 623, 649, 823
Alw21I GWGCWC 1 cut(s) 825
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 6 cut(s) 81, 144, 236, 491, 746, 1076
Ama87I CYCGRG 1 cut(s) 888
Aor51HI AGCGCT 1 cut(s) 279
AoxI GGCC 2 cut(s) 198, 227
ApeKI GCWGC 3 cut(s) 280, 464, 1155
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
AscI GGCGCGCC 1 cut(s) 1216
AseI ATTAAT 2 cut(s) 30, 93
AspLEI GCGC 5 cut(s) 265, 280, 549, 1218, 1220
AspS9I GGNCC 5 cut(s) 227, 459, 780, 810, 1144
AsuC2I CCSGG 3 cut(s) 889, 890, 1148
AsuHPI GGTGA 2 cut(s) 1006, 1358
AvaI CYCGRG 1 cut(s) 888
AvaII GGWCC 4 cut(s) 459, 780, 810, 1144
BanI GGYRCC 1 cut(s) 1381
BanII GRGCYC 1 cut(s) 825
BauI CACGAG 1 cut(s) 1341
BbsI GAAGAC 1 cut(s) 1273
Bbv12I GWGCWC 1 cut(s) 825
BbvI GCAGC 3 cut(s) 267, 451, 1167
BccI CCATC 4 cut(s) 425, 509, 949, 1432
BcnI CCSGG 3 cut(s) 889, 890, 1148
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 2 cut(s) 1038, 1085
BfmI CTRYAG 1 cut(s) 1299
BfoI RGCGCY 1 cut(s) 281
BglII AGATCT 1 cut(s) 100
BisI GCNGC 4 cut(s) 281, 465, 1156, 1275
BlsI GCNGC 4 cut(s) 282, 466, 1157, 1276
Bme1390I CCNGG 3 cut(s) 889, 890, 1148
Bme18I GGWCC 4 cut(s) 459, 780, 810, 1144
BmeT110I CYCGRG 1 cut(s) 888
BmgT120I GGNCC 5 cut(s) 227, 459, 780, 810, 1144
BmiI GGNNCC 4 cut(s) 905, 1146, 1377, 1383
BmrFI CCNGG 3 cut(s) 889, 890, 1148
BmsI GCATC 2 cut(s) 319, 619
BpiI GAAGAC 1 cut(s) 1273
Bpu10I CCTNAGC 1 cut(s) 624
BpuEI CTTGAG 2 cut(s) 89, 584
BpuMI CCSGG 3 cut(s) 889, 890, 1148
BsaJI CCNNGG 5 cut(s) 783, 888, 889, 1202, 1250
BsaWI WCCGGW 1 cut(s) 1378
BsaXI ACNNNNNCTCC 2 cut(s) 1403, 1433
Bse1I ACTGG 1 cut(s) 480
Bse3DI GCAATG 2 cut(s) 682, 1269
BseDI CCNNGG 5 cut(s) 783, 888, 889, 1202, 1250
BseGI GGATG 8 cut(s) 250, 484, 634, 928, 1048, 1071, 1138, 1403
BseMI GCAATG 2 cut(s) 682, 1269
BseNI ACTGG 1 cut(s) 480
BsePI GCGCGC 1 cut(s) 1216
BseRI GAGGAG 2 cut(s) 1377, 1380
BseXI GCAGC 3 cut(s) 267, 451, 1167
Bsh1236I CGCG 2 cut(s) 549, 1218
BshFI GGCC 2 cut(s) 200, 229
BshNI GGYRCC 1 cut(s) 1381
BsiHKAI GWGCWC 1 cut(s) 825
BsiHKCI CYCGRG 1 cut(s) 888
BsiSI CCGG 3 cut(s) 889, 1148, 1379
BsmAI GTCTC 1 cut(s) 223
BsnI GGCC 2 cut(s) 200, 229
BsoBI CYCGRG 1 cut(s) 888
Bsp1286I GDGCHC 1 cut(s) 825
Bsp1407I TGTACA 1 cut(s) 720
Bsp19I CCATGG 3 cut(s) 783, 1202, 1250
BspACI CCGC 5 cut(s) 338, 462, 673, 813, 1274
BspANI GGCC 2 cut(s) 200, 229
BspFNI CGCG 2 cut(s) 549, 1218
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 4 cut(s) 905, 1146, 1377, 1383
BspPI GGATC 6 cut(s) 81, 144, 236, 491, 746, 1076
BspT107I GGYRCC 1 cut(s) 1381
BsrDI GCAATG 2 cut(s) 682, 1269
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 1 cut(s) 480
BssECI CCNNGG 5 cut(s) 783, 888, 889, 1202, 1250
BssHII GCGCGC 1 cut(s) 1216
BssSI CACGAG 1 cut(s) 1341
BssT1I CCWWGG 3 cut(s) 783, 1202, 1250
Bst2BI CACGAG 1 cut(s) 1341
Bst4CI ACNGT 3 cut(s) 217, 518, 780
Bst6I CTCTTC 4 cut(s) 378, 722, 792, 1016
BstAPI GCANNNNNTGC 2 cut(s) 15, 532
BstAUI TGTACA 1 cut(s) 720
BstC8I GCNNGC 3 cut(s) 7, 175, 1218
BstDEI CTNAG 2 cut(s) 624, 1128
BstDSI CCRYGG 3 cut(s) 783, 1202, 1250
BstF5I GGATG 8 cut(s) 250, 484, 634, 928, 1048, 1071, 1138, 1403
BstFNI CGCG 2 cut(s) 549, 1218
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 5 cut(s) 265, 280, 549, 1218, 1220
BstMAI GTCTC 1 cut(s) 223
BstMWI GCNNNNNNNGC 4 cut(s) 15, 277, 532, 629
BstNSI RCATGY 4 cut(s) 530, 556, 710, 1471
BstSCI CCNGG 3 cut(s) 887, 888, 1146
BstSFI CTRYAG 1 cut(s) 1299
BstUI CGCG 2 cut(s) 549, 1218
BstV1I GCAGC 3 cut(s) 267, 451, 1167
BstV2I GAAGAC 1 cut(s) 1273
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 2 cut(s) 200, 229
BtgI CCRYGG 3 cut(s) 783, 1202, 1250
BtsCI GGATG 8 cut(s) 250, 484, 634, 928, 1048, 1071, 1138, 1403
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 1 cut(s) 1107
Cac8I GCNNGC 3 cut(s) 7, 175, 1218
CciI TCATGA 1 cut(s) 453
CfoI GCGC 5 cut(s) 265, 280, 549, 1218, 1220
Cfr13I GGNCC 5 cut(s) 227, 459, 780, 810, 1144
Cfr9I CCCGGG 1 cut(s) 888
Csp6I GTAC 3 cut(s) 473, 721, 1315
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviQI GTAC 3 cut(s) 473, 721, 1315
DdeI CTNAG 2 cut(s) 624, 1128
DraIII CACNNNGTG 1 cut(s) 1346
Eam1104I CTCTTC 4 cut(s) 378, 722, 792, 1016
EarI CTCTTC 4 cut(s) 378, 722, 792, 1016
EciI GGCGGA 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 823
Eco130I CCWWGG 3 cut(s) 783, 1202, 1250
Eco147I AGGCCT 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 825
Eco47I GGWCC 4 cut(s) 459, 780, 810, 1144
Eco47III AGCGCT 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 823
Eco57I CTGAAG 3 cut(s) 490, 722, 1225
Eco88I CYCGRG 1 cut(s) 888
EcoICRI GAGCTC 1 cut(s) 823
EcoO109I RGGNCCY 1 cut(s) 1144
EcoT14I CCWWGG 3 cut(s) 783, 1202, 1250
EcoT22I ATGCAT 2 cut(s) 334, 1469
EcoT38I GRGCYC 1 cut(s) 825
ErhI CCWWGG 3 cut(s) 783, 1202, 1250
FauI CCCGC 1 cut(s) 680
FauNDI CATATG 1 cut(s) 806
Fnu4HI GCNGC 4 cut(s) 281, 465, 1156, 1275
FokI GGATG 8 cut(s) 237, 491, 641, 935, 1055, 1058, 1145, 1390
FriOI GRGCYC 1 cut(s) 825
Fsp4HI GCNGC 4 cut(s) 281, 465, 1156, 1275
FspBI CTAG 2 cut(s) 1038, 1085
GlaI GCGC 5 cut(s) 264, 279, 548, 1217, 1219
GluI GCNGC 4 cut(s) 281, 465, 1156, 1275
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 2 cut(s) 200, 229
HapII CCGG 3 cut(s) 889, 1148, 1379
HhaI GCGC 5 cut(s) 265, 280, 549, 1218, 1220
Hin6I GCGC 5 cut(s) 263, 278, 547, 1216, 1218
HinP1I GCGC 5 cut(s) 263, 278, 547, 1216, 1218
HinfI GANTC 9 cut(s) 362, 568, 730, 837, 935, 949, 969, 1349, 1412
HpaII CCGG 3 cut(s) 889, 1148, 1379
HphI GGTGA 2 cut(s) 1006, 1358
Hpy166II GTNNAC 3 cut(s) 688, 1296, 1473
Hpy188I TCNGA 5 cut(s) 954, 1244, 1308, 1363, 1411
Hpy188III TCNNGA 6 cut(s) 43, 106, 454, 590, 847, 946
Hpy8I GTNNAC 3 cut(s) 688, 1296, 1473
HpyAV CCTTC 7 cut(s) 47, 110, 190, 232, 255, 352, 1368
HpyCH4III ACNGT 3 cut(s) 217, 518, 780
HpyCH4V TGCA 9 cut(s) 18, 332, 526, 632, 706, 857, 1171, 1194, 1467
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 277, 532, 629
HpyF3I CTNAG 2 cut(s) 624, 1128
HspAI GCGC 5 cut(s) 263, 278, 547, 1216, 1218
LmnI GCTCC 3 cut(s) 493, 909, 980
Lsp1109I GCAGC 3 cut(s) 267, 451, 1167
LweI GCATC 2 cut(s) 319, 619
MaeI CTAG 2 cut(s) 1038, 1085
MaeIII GTNAC 4 cut(s) 397, 640, 994, 1091
MfeI CAATTG 1 cut(s) 1477
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 825
MluCI AATT 5 cut(s) 27, 90, 1117, 1164, 1477
MlyI GAGTC 3 cut(s) 739, 944, 1406
MmeI TCCRAC 2 cut(s) 693, 1434
Mph1103I ATGCAT 2 cut(s) 334, 1469
MseI TTAA 4 cut(s) 30, 93, 557, 1077
MslI CAYNNNNRTG 1 cut(s) 557
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 3 cut(s) 889, 1148, 1379
MspR9I CCNGG 3 cut(s) 889, 890, 1148
MunI CAATTG 1 cut(s) 1477
MvnI CGCG 2 cut(s) 549, 1218
MwoI GCNNNNNNNGC 4 cut(s) 15, 277, 532, 629
NciI CCSGG 3 cut(s) 889, 890, 1148
NcoI CCATGG 3 cut(s) 783, 1202, 1250
NdeI CATATG 1 cut(s) 806
NlaIV GGNNCC 4 cut(s) 905, 1146, 1377, 1383
NmuCI GTSAC 3 cut(s) 640, 994, 1091
NsiI ATGCAT 2 cut(s) 334, 1469
NspI RCATGY 4 cut(s) 530, 556, 710, 1471
PagI TCATGA 1 cut(s) 453
PalAI GGCGCGCC 1 cut(s) 1216
PauI GCGCGC 1 cut(s) 1216
PceI AGGCCT 1 cut(s) 200
PciI ACATGT 1 cut(s) 552
PfeI GAWTC 6 cut(s) 362, 568, 837, 949, 969, 1349
PkrI GCNGC 4 cut(s) 282, 466, 1157, 1276
PleI GAGTC 3 cut(s) 738, 943, 1406
PpsI GAGTC 3 cut(s) 738, 943, 1406
PpuMI RGGWCCY 1 cut(s) 1144
PscI ACATGT 1 cut(s) 552
PshBI ATTAAT 2 cut(s) 30, 93
Psp124BI GAGCTC 1 cut(s) 825
Psp5II RGGWCCY 1 cut(s) 1144
PspN4I GGNNCC 4 cut(s) 905, 1146, 1377, 1383
PspPI GGNCC 5 cut(s) 227, 459, 780, 810, 1144
PspPPI RGGWCCY 1 cut(s) 1144
PsuI RGATCY 1 cut(s) 100
PteI GCGCGC 1 cut(s) 1216
RsaI GTAC 3 cut(s) 474, 722, 1316
RsaNI GTAC 3 cut(s) 473, 721, 1315
RseI CAYNNNNRTG 1 cut(s) 557
SacI GAGCTC 1 cut(s) 825
SaqAI TTAA 4 cut(s) 30, 93, 557, 1077
SatI GCNGC 4 cut(s) 281, 465, 1156, 1275
Sau96I GGNCC 5 cut(s) 227, 459, 780, 810, 1144
SchI GAGTC 3 cut(s) 739, 944, 1406
ScrFI CCNGG 3 cut(s) 889, 890, 1148
SduI GDGCHC 1 cut(s) 825
SfaNI GCATC 2 cut(s) 319, 619
SfcI CTRYAG 1 cut(s) 1299
SgsI GGCGCGCC 1 cut(s) 1216
SinI GGWCC 4 cut(s) 459, 780, 810, 1144
SmaI CCCGGG 1 cut(s) 890
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 2 cut(s) 104, 563
SmoI CTYRAG 2 cut(s) 104, 563
Sse9I AATT 5 cut(s) 27, 90, 1117, 1164, 1477
SseBI AGGCCT 1 cut(s) 200
SsiI CCGC 5 cut(s) 338, 462, 673, 813, 1274
SspMI CTAG 2 cut(s) 1038, 1085
SstI GAGCTC 1 cut(s) 825
StuI AGGCCT 1 cut(s) 200
StyD4I CCNGG 3 cut(s) 887, 888, 1146
StyI CCWWGG 3 cut(s) 783, 1202, 1250
TaaI ACNGT 3 cut(s) 217, 518, 780
TaqI TCGA 3 cut(s) 155, 766, 1061
TasI AATT 5 cut(s) 27, 90, 1117, 1164, 1477
TatI WGTACW 2 cut(s) 472, 720
TauI GCSGC 1 cut(s) 1277
TfiI GAWTC 6 cut(s) 362, 568, 837, 949, 969, 1349
Tru1I TTAA 4 cut(s) 30, 93, 557, 1077
Tru9I TTAA 4 cut(s) 30, 93, 557, 1077
TscAI CASTG 1 cut(s) 1107
TseFI GTSAC 3 cut(s) 640, 994, 1091
TseI GCWGC 3 cut(s) 280, 464, 1155
Tsp45I GTSAC 3 cut(s) 640, 994, 1091
TspDTI ATGAA 9 cut(s) 48, 111, 275, 354, 368, 430, 1003, 1258, 1392
TspMI CCCGGG 1 cut(s) 888
TspRI CASTG 1 cut(s) 1107
VpaK11BI GGWCC 4 cut(s) 459, 780, 810, 1144
VspI ATTAAT 2 cut(s) 30, 93
XceI RCATGY 4 cut(s) 530, 556, 710, 1471
XcmI CCANNNNNNNNNTGG 1 cut(s) 1209
XmaI CCCGGG 1 cut(s) 888
XspI CTAG 2 cut(s) 1038, 1085
Zsp2I ATGCAT 2 cut(s) 334, 1469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.