Rh4AG281500

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002269
Physical Location & Seq
Reverse (-)
59652568 .. 59653203
636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG281500.1

Sequence Viewer

Length: 501 bp
ATGGCTGACATGAACTGGATTATTGAAGTTAATGAAGATCTTAATAGGATGGAAGGAGCAGTGGAAGGGGTCTATTTACAAACTTCCAGCCGGCATTGCAGACATAAACAAGAAGGCCTACAAGCCCCAGACAGTCTCCTTCGGCCCTCACACTTCCTCAAAAGATGTGGAAAACCTGTTGAACTGTTTGCAGATGCACTTGCTGAAGTAGTGGAAGATTTGAAGGACTCATACAATCCTCTCGAGCCAGTGTGGAGAGATAATACAGCTAGATTTTTGCAACTCATGTTTCTGGATGGCTGTTTCATGCTTGAAATCTTGAGGACTGCTTCTCATATACTTGATGATTATGCTCCTACTGATCCTATCTTCAGCAAGCACGGGAAGTTTCATGTCATGCCCTTCATCAAACGCGACATGTTAATGCTCGAGAATCAGTTGCCAATGCTTGTTCTTGTCAAGCTGGTCGCAGTTGAAACTGATAAAGCTAAGTACCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

166

Amino Acids

19.16

Weight (kDa)

5.49

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 51 - 160 1e-22 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 414
AclWI GGATC 1 cut(s) 356
AcuI CTGAAG 2 cut(s) 225, 355
AfaI GTAC 1 cut(s) 494
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 5 cut(s) 26, 182, 223, 314, 476
AluBI AGCT 3 cut(s) 269, 463, 488
AluI AGCT 3 cut(s) 269, 463, 488
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 1 cut(s) 356
Ama87I CYCGRG 2 cut(s) 242, 428
AoxI GGCC 2 cut(s) 115, 143
AspS9I GGNCC 1 cut(s) 144
AvaI CYCGRG 2 cut(s) 242, 428
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BccI CCATC 2 cut(s) 43, 290
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 1 cut(s) 270
BglII AGATCT 1 cut(s) 37
BmeT110I CYCGRG 2 cut(s) 242, 428
BmgT120I GGNCC 1 cut(s) 144
BmsI GCATC 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 340
Bse118I RCCGGY 1 cut(s) 90
Bse1I ACTGG 2 cut(s) 20, 248
Bse3DI GCAATG 1 cut(s) 94
BseGI GGATG 2 cut(s) 54, 301
BseMI GCAATG 1 cut(s) 94
BseNI ACTGG 2 cut(s) 20, 248
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 2 cut(s) 117, 145
BsiHKCI CYCGRG 2 cut(s) 242, 428
BsiSI CCGG 1 cut(s) 91
BsmAI GTCTC 1 cut(s) 140
BsnI GGCC 2 cut(s) 117, 145
BsoBI CYCGRG 2 cut(s) 242, 428
Bsp143I GATC 2 cut(s) 37, 361
BspANI GGCC 2 cut(s) 117, 145
BspFNI CGCG 1 cut(s) 414
BspPI GGATC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 90
BsrI ACTGG 2 cut(s) 20, 248
BssAI RCCGGY 1 cut(s) 90
BssMI GATC 2 cut(s) 37, 361
Bst4CI ACNGT 2 cut(s) 134, 186
BstC8I GCNNGC 2 cut(s) 92, 377
BstDEI CTNAG 1 cut(s) 489
BstF5I GGATG 2 cut(s) 54, 301
BstFNI CGCG 1 cut(s) 414
BstKTI GATC 2 cut(s) 40, 364
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 2 cut(s) 37, 361
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNSI RCATGY 1 cut(s) 421
BstUI CGCG 1 cut(s) 414
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 117, 145
BtsCI GGATG 2 cut(s) 54, 301
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 66, 255
Cac8I GCNNGC 2 cut(s) 92, 377
Cfr10I RCCGGY 1 cut(s) 90
Cfr13I GGNCC 1 cut(s) 144
Csp6I GTAC 1 cut(s) 493
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CviAII CATG 6 cut(s) 10, 286, 307, 392, 397, 418
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 1 cut(s) 489
DpnI GATC 2 cut(s) 39, 363
DpnII GATC 2 cut(s) 37, 361
Eco147I AGGCCT 1 cut(s) 117
Eco57I CTGAAG 2 cut(s) 225, 355
Eco88I CYCGRG 2 cut(s) 242, 428
FaeI CATG 6 cut(s) 13, 289, 310, 395, 400, 421
FatI CATG 6 cut(s) 9, 285, 306, 391, 396, 417
FokI GGATG 2 cut(s) 61, 308
FspBI CTAG 1 cut(s) 270
HaeIII GGCC 2 cut(s) 117, 145
HapII CCGG 1 cut(s) 91
Hin1II CATG 6 cut(s) 13, 289, 310, 395, 400, 421
HinfI GANTC 2 cut(s) 227, 433
HpaII CCGG 1 cut(s) 91
Hpy188III TCNNGA 4 cut(s) 242, 293, 319, 430
HpyAV CCTTC 6 cut(s) 47, 59, 107, 149, 217, 412
HpyCH4III ACNGT 2 cut(s) 134, 186
HpyCH4V TGCA 4 cut(s) 99, 191, 197, 280
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpyF3I CTNAG 1 cut(s) 489
Hsp92II CATG 6 cut(s) 13, 289, 310, 395, 400, 421
KroI GCCGGC 1 cut(s) 90
KroNI GCCGGC 1 cut(s) 92
Kzo9I GATC 2 cut(s) 37, 361
LmnI GCTCC 2 cut(s) 56, 358
LpnPI CCDG 7 cut(s) 100, 104, 141, 189, 261, 278, 449
LweI GCATC 1 cut(s) 184
MaeI CTAG 1 cut(s) 270
MalI GATC 2 cut(s) 39, 363
MboI GATC 2 cut(s) 37, 361
MboII GAAGA 3 cut(s) 47, 227, 361
MflI RGATCY 1 cut(s) 37
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 4 cut(s) 157, 167, 249, 315
MroNI GCCGGC 1 cut(s) 90
MseI TTAA 3 cut(s) 30, 42, 422
MslI CAYNNNNRTG 1 cut(s) 422
MspI CCGG 1 cut(s) 91
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 96
NaeI GCCGGC 1 cut(s) 92
NdeII GATC 2 cut(s) 37, 361
NgoMIV GCCGGC 1 cut(s) 90
NlaIII CATG 6 cut(s) 13, 289, 310, 395, 400, 421
NspI RCATGY 1 cut(s) 421
PaeR7I CTCGAG 2 cut(s) 242, 428
PceI AGGCCT 1 cut(s) 117
PciI ACATGT 1 cut(s) 417
PdiI GCCGGC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 433
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PscI ACATGT 1 cut(s) 417
PspPI GGNCC 1 cut(s) 144
PsuI RGATCY 1 cut(s) 37
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
RseI CAYNNNNRTG 1 cut(s) 422
SaqAI TTAA 3 cut(s) 30, 42, 422
Sau3AI GATC 2 cut(s) 37, 361
Sau96I GGNCC 1 cut(s) 144
SchI GAGTC 1 cut(s) 221
SetI ASST 4 cut(s) 178, 271, 465, 490
SfaNI GCATC 1 cut(s) 184
Sfr274I CTCGAG 2 cut(s) 242, 428
SlaI CTCGAG 2 cut(s) 242, 428
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 3 cut(s) 242, 319, 428
SmoI CTYRAG 3 cut(s) 242, 319, 428
SseBI AGGCCT 1 cut(s) 117
SspMI CTAG 1 cut(s) 270
StuI AGGCCT 1 cut(s) 117
TaaI ACNGT 2 cut(s) 134, 186
TaqI TCGA 2 cut(s) 243, 429
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 3 cut(s) 30, 42, 422
Tru9I TTAA 3 cut(s) 30, 42, 422
TscAI CASTG 2 cut(s) 66, 255
TspDTI ATGAA 5 cut(s) 26, 48, 295, 380, 394
TspRI CASTG 2 cut(s) 66, 255
XceI RCATGY 1 cut(s) 421
XhoI CTCGAG 2 cut(s) 242, 428
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.