Rh4CG302500

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002269
Physical Location & Seq
Reverse (-)
56306738 .. 56308241
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG302500.1

Sequence Viewer

Length: 1413 bp
ATGGCTGGCACCGGATGGATTATTCAAATTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAACAGGATCACTGGAATAAGGGGTCAATCTACAAACTACCAGCTAGCCTCACAGACATGAACAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGACCTTACCACTACAATTCCTTAAATCCAATGGAGGAGCACAAGCACCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGCCTGTTGAGTTGTTTGTAGATGCATTGGCTGAAGTAGAGAATGAATTGAAGGATTCATATACTCTGCTTCGTTCTGTGCCAAAAGAAATTACCGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTGGAAATCGTAAGGAGTGCTGCTCATGTACCGGATGATTATGCTCCTAATGATCCCATCTTCGGAGAACATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAACATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTAATTGTCAAGTTCCTCTGCTCCAGTAACTTTTCTCCAGACATGGGCAAGTGTGTGCATGTGTTGGACATGTACAGGAAGAGTCTGCTTCAGCATGAGCCTAATCCCAAATTTATTCGCAAAATAAGGTCAAGTGAGCATGACGATGAGATCATCTGGTCCGCAACAGCGCTCAATGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCGCCCGGGGAGTGTTGAGGCTCCCTGTTGTTGTTGTGGATGACACCACCGAGTCCATGTTCTTGAATCTGATGGCGTTTGAAAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCTATGGATGTTGCCCTCTTACACTCGAAAGGGATCATCCAGAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTCTTCAATTCACTGTCTAAGGATGTGACACAGGACCCAGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATGAGTGGCGCGCCAATCTTATTCACACTTACTTCCGAAATCCATGGGCTATTATCTCTTTCATTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATGCCATATATCCCTATTACCATCCACTAAATGATTCTTCTCCTTCCACTGTCATTCTCGTTCCGGCTCCTCCTCCAAAACATTCATCCTCCGACTCCACCTCCCCATCGACACTTGTGATTTCTTTCATCCTTGTTACCATGGTATGCATGCTGACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

470

Amino Acids

53.31

Weight (kDa)

6.34

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 409 1.7e-115 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 3 cut(s) 213, 215, 1152
AciI CCGC 2 cut(s) 211, 747
AclWI GGATC 3 cut(s) 81, 428, 1010
AcsI RAATTY 1 cut(s) 695
AcuI CTGAAG 2 cut(s) 297, 659
AfaI GTAC 2 cut(s) 411, 659
AfeI AGCGCT 1 cut(s) 756
AfiI CCNNNNNNNGG 2 cut(s) 443, 629
AflIII ACRYGT 2 cut(s) 489, 654
AluBI AGCT 5 cut(s) 110, 535, 560, 586, 1238
AluI AGCT 5 cut(s) 110, 535, 560, 586, 1238
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 3 cut(s) 81, 428, 1010
Ama87I CYCGRG 1 cut(s) 822
Aor51HI AGCGCT 1 cut(s) 756
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 3 cut(s) 217, 401, 1089
ApoI RAATTY 1 cut(s) 695
ArsI GACNNNNNNTTYG 2 cut(s) 800, 832
AscI GGCGCGCC 1 cut(s) 1150
AseI ATTAAT 1 cut(s) 30
AspLEI GCGC 5 cut(s) 215, 217, 757, 1152, 1154
AspS9I GGNCC 3 cut(s) 164, 744, 1078
AsuC2I CCSGG 2 cut(s) 823, 824
AsuHPI GGTGA 1 cut(s) 940
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 822
AvaII GGWCC 3 cut(s) 164, 744, 1078
BanI GGYRCC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 1207
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 3 cut(s) 204, 388, 1101
BccI CCATC 6 cut(s) 9, 362, 446, 883, 1278, 1363
BceAI ACGGC 1 cut(s) 1120
BcnI CCSGG 2 cut(s) 823, 824
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 2 cut(s) 1126, 1233
BfoI RGCGCY 1 cut(s) 758
BglII AGATCT 1 cut(s) 37
BisI GCNGC 3 cut(s) 218, 402, 1090
BlsI GCNGC 3 cut(s) 219, 403, 1091
Bme1390I CCNGG 2 cut(s) 823, 824
Bme18I GGWCC 3 cut(s) 164, 744, 1078
BmeT110I CYCGRG 1 cut(s) 822
BmgT120I GGNCC 3 cut(s) 164, 744, 1078
BmiI GGNNCC 5 cut(s) 10, 461, 839, 1080, 1317
BmrFI CCNGG 2 cut(s) 823, 824
BmsI GCATC 1 cut(s) 256
BmtI GCTAGC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 1207
BplI GAGNNNNNCTC 2 cut(s) 388, 420
BpmI CTGGAG 2 cut(s) 592, 606
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 26, 521
BpuMI CCSGG 2 cut(s) 823, 824
BsaJI CCNNGG 5 cut(s) 822, 823, 1136, 1184, 1389
BsaWI WCCGGW 2 cut(s) 11, 412
BsaXI ACNNNNNCTCC 2 cut(s) 1334, 1364
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 629
Bse1I ACTGG 3 cut(s) 83, 609, 1249
Bse3DI GCAATG 1 cut(s) 1203
BseDI CCNNGG 5 cut(s) 822, 823, 1136, 1184, 1389
BseLI CCNNNNNNNGG 2 cut(s) 443, 629
BseMI GCAATG 1 cut(s) 1203
BseMII CTCAG 1 cut(s) 1253
BseNI ACTGG 3 cut(s) 83, 609, 1249
BsePI GCGCGC 2 cut(s) 213, 1150
BseRI GAGGAG 3 cut(s) 212, 1308, 1311
BseXI GCAGC 3 cut(s) 204, 388, 1101
Bsh1236I CGCG 3 cut(s) 213, 215, 1152
BshFI GGCC 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 4 cut(s) 12, 413, 823, 1313
BslI CCNNNNNNNGG 2 cut(s) 443, 629
BsmAI GTCTC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 1018
BsnI GGCC 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 822
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 5 cut(s) 37, 73, 433, 735, 1002
Bsp19I CCATGG 2 cut(s) 1184, 1389
BspACI CCGC 2 cut(s) 211, 747
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 1 cut(s) 1252
BspFNI CGCG 3 cut(s) 213, 215, 1152
BspLI GGNNCC 5 cut(s) 10, 461, 839, 1080, 1317
BspMAI CTGCAG 1 cut(s) 1130
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 3 cut(s) 81, 428, 1010
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 1203
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 3 cut(s) 83, 609, 1249
BssECI CCNNGG 5 cut(s) 822, 823, 1136, 1184, 1389
BssHII GCGCGC 2 cut(s) 213, 1150
BssMI GATC 5 cut(s) 37, 73, 433, 735, 1002
BssT1I CCWWGG 2 cut(s) 1184, 1389
Bst4CI ACNGT 3 cut(s) 154, 1059, 1300
Bst6I CTCTTC 3 cut(s) 659, 950, 1052
BstAUI TGTACA 1 cut(s) 657
BstC8I GCNNGC 5 cut(s) 7, 112, 215, 1152, 1400
BstDEI CTNAG 3 cut(s) 561, 1062, 1239
BstDSI CCRYGG 3 cut(s) 1136, 1184, 1389
BstFNI CGCG 3 cut(s) 213, 215, 1152
BstH2I RGCGCY 1 cut(s) 758
BstHHI GCGC 5 cut(s) 215, 217, 757, 1152, 1154
BstKTI GATC 5 cut(s) 40, 76, 436, 738, 1005
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 5 cut(s) 37, 73, 433, 735, 1002
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNSI RCATGY 4 cut(s) 493, 647, 658, 1402
BstSCI CCNGG 2 cut(s) 821, 822
BstSFI CTRYAG 2 cut(s) 1126, 1233
BstUI CGCG 3 cut(s) 213, 215, 1152
BstV1I GCAGC 3 cut(s) 204, 388, 1101
BstV2I GAAGAC 1 cut(s) 1207
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 1 cut(s) 137
BtgI CCRYGG 3 cut(s) 1136, 1184, 1389
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 4 cut(s) 76, 1041, 1055, 1296
Cac8I GCNNGC 5 cut(s) 7, 112, 215, 1152, 1400
CfoI GCGC 5 cut(s) 215, 217, 757, 1152, 1154
Cfr13I GGNCC 3 cut(s) 164, 744, 1078
Cfr9I CCCGGG 1 cut(s) 822
Csp6I GTAC 2 cut(s) 410, 658
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 2 cut(s) 410, 658
DdeI CTNAG 3 cut(s) 561, 1062, 1239
DpnI GATC 5 cut(s) 39, 75, 435, 737, 1004
DpnII GATC 5 cut(s) 37, 73, 433, 735, 1002
Eam1104I CTCTTC 3 cut(s) 659, 950, 1052
EarI CTCTTC 3 cut(s) 659, 950, 1052
Eco130I CCWWGG 2 cut(s) 1184, 1389
Eco147I AGGCCT 1 cut(s) 137
Eco47I GGWCC 3 cut(s) 164, 744, 1078
Eco47III AGCGCT 1 cut(s) 756
Eco57I CTGAAG 2 cut(s) 297, 659
Eco88I CYCGRG 1 cut(s) 822
EcoO109I RGGNCCY 1 cut(s) 1078
EcoT14I CCWWGG 2 cut(s) 1184, 1389
EcoT22I ATGCAT 2 cut(s) 271, 1400
ErhI CCWWGG 2 cut(s) 1184, 1389
Fnu4HI GCNGC 3 cut(s) 218, 402, 1090
Fsp4HI GCNGC 3 cut(s) 218, 402, 1090
FspBI CTAG 1 cut(s) 111
GlaI GCGC 5 cut(s) 214, 216, 756, 1151, 1153
GluI GCNGC 3 cut(s) 218, 402, 1090
GsuI CTGGAG 2 cut(s) 592, 606
HaeII RGCGCY 1 cut(s) 758
HaeIII GGCC 1 cut(s) 137
HapII CCGG 4 cut(s) 12, 413, 823, 1313
HhaI GCGC 5 cut(s) 215, 217, 757, 1152, 1154
Hin6I GCGC 5 cut(s) 213, 215, 755, 1150, 1152
HinP1I GCGC 5 cut(s) 213, 215, 755, 1150, 1152
HinfI GANTC 9 cut(s) 299, 505, 667, 771, 869, 883, 903, 1283, 1343
HpaII CCGG 4 cut(s) 12, 413, 823, 1313
HphI GGTGA 1 cut(s) 940
Hpy166II GTNNAC 1 cut(s) 1230
Hpy188I TCNGA 6 cut(s) 164, 446, 888, 1178, 1242, 1342
Hpy188III TCNNGA 6 cut(s) 43, 527, 623, 781, 880, 1009
Hpy8I GTNNAC 1 cut(s) 1230
HpyAV CCTTC 7 cut(s) 47, 127, 169, 289, 563, 1103, 1302
HpyCH4III ACNGT 3 cut(s) 154, 1059, 1300
HpyCH4V TGCA 7 cut(s) 269, 352, 643, 791, 1105, 1128, 1398
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 3 cut(s) 561, 1062, 1239
HspAI GCGC 5 cut(s) 213, 215, 755, 1150, 1152
Kzo9I GATC 5 cut(s) 37, 73, 433, 735, 1002
LmnI GCTCC 7 cut(s) 199, 430, 465, 611, 843, 914, 1321
Lsp1109I GCAGC 3 cut(s) 204, 388, 1101
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 7 cut(s) 485, 577, 611, 928, 1025, 1069, 1384
MalI GATC 5 cut(s) 39, 75, 435, 737, 1004
MboI GATC 5 cut(s) 37, 73, 433, 735, 1002
MboII GAAGA 7 cut(s) 47, 433, 676, 937, 1039, 1207, 1278
MfeI CAATTG 1 cut(s) 1408
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 9 cut(s) 27, 178, 290, 333, 588, 695, 1051, 1098, 1408
MlyI GAGTC 3 cut(s) 676, 878, 1337
MmeI TCCRAC 2 cut(s) 630, 1365
Mph1103I ATGCAT 2 cut(s) 271, 1400
MseI TTAA 3 cut(s) 30, 185, 494
MslI CAYNNNNRTG 3 cut(s) 122, 494, 729
MspI CCGG 4 cut(s) 12, 413, 823, 1313
MspR9I CCNGG 2 cut(s) 823, 824
MunI CAATTG 1 cut(s) 1408
Mva1269I GAATGC 1 cut(s) 1018
MvnI CGCG 3 cut(s) 213, 215, 1152
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 2 cut(s) 823, 824
NcoI CCATGG 2 cut(s) 1184, 1389
NdeII GATC 5 cut(s) 37, 73, 433, 735, 1002
NheI GCTAGC 1 cut(s) 110
NlaIV GGNNCC 5 cut(s) 10, 461, 839, 1080, 1317
NmuCI GTSAC 5 cut(s) 485, 577, 928, 1025, 1069
NsiI ATGCAT 2 cut(s) 271, 1400
NspI RCATGY 4 cut(s) 493, 647, 658, 1402
PaeI GCATGC 1 cut(s) 1402
PalAI GGCGCGCC 1 cut(s) 1150
PauI GCGCGC 2 cut(s) 213, 1150
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 2 cut(s) 489, 654
PctI GAATGC 1 cut(s) 1018
PfeI GAWTC 6 cut(s) 299, 505, 771, 883, 903, 1283
PkrI GCNGC 3 cut(s) 219, 403, 1091
PleI GAGTC 3 cut(s) 675, 877, 1337
PpsI GAGTC 3 cut(s) 675, 877, 1337
PpuMI RGGWCCY 1 cut(s) 1078
PscI ACATGT 2 cut(s) 489, 654
PshBI ATTAAT 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 1078
PspN4I GGNNCC 5 cut(s) 10, 461, 839, 1080, 1317
PspPI GGNCC 3 cut(s) 164, 744, 1078
PspPPI RGGWCCY 1 cut(s) 1078
PstI CTGCAG 1 cut(s) 1130
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 2 cut(s) 213, 1150
RsaI GTAC 2 cut(s) 411, 659
RsaNI GTAC 2 cut(s) 410, 658
RseI CAYNNNNRTG 3 cut(s) 122, 494, 729
SaqAI TTAA 3 cut(s) 30, 185, 494
SatI GCNGC 3 cut(s) 218, 402, 1090
Sau3AI GATC 5 cut(s) 37, 73, 433, 735, 1002
Sau96I GGNCC 3 cut(s) 164, 744, 1078
SchI GAGTC 3 cut(s) 676, 878, 1337
ScrFI CCNGG 2 cut(s) 823, 824
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 2 cut(s) 1126, 1233
SgsI GGCGCGCC 1 cut(s) 1150
SinI GGWCC 3 cut(s) 164, 744, 1078
SmaI CCCGGG 1 cut(s) 824
SmiMI CAYNNNNRTG 3 cut(s) 122, 494, 729
SmlI CTYRAG 2 cut(s) 41, 500
SmoI CTYRAG 2 cut(s) 41, 500
SphI GCATGC 1 cut(s) 1402
Sse9I AATT 9 cut(s) 27, 178, 290, 333, 588, 695, 1051, 1098, 1408
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 2 cut(s) 211, 747
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 821, 822
StyI CCWWGG 2 cut(s) 1184, 1389
TaaI ACNGT 3 cut(s) 154, 1059, 1300
TaqI TCGA 2 cut(s) 995, 1358
TasI AATT 9 cut(s) 27, 178, 290, 333, 588, 695, 1051, 1098, 1408
TatI WGTACW 1 cut(s) 657
TfiI GAWTC 6 cut(s) 299, 505, 771, 883, 903, 1283
Tru1I TTAA 3 cut(s) 30, 185, 494
Tru9I TTAA 3 cut(s) 30, 185, 494
TscAI CASTG 4 cut(s) 83, 1041, 1062, 1303
TseFI GTSAC 5 cut(s) 485, 577, 928, 1025, 1069
TseI GCWGC 3 cut(s) 217, 401, 1089
Tsp45I GTSAC 5 cut(s) 485, 577, 928, 1025, 1069
TspMI CCCGGG 1 cut(s) 822
TspRI CASTG 4 cut(s) 83, 1041, 1062, 1303
VpaK11BI GGWCC 3 cut(s) 164, 744, 1078
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 1 cut(s) 695
XceI RCATGY 4 cut(s) 493, 647, 658, 1402
XmaI CCCGGG 1 cut(s) 822
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 2 cut(s) 271, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.