Rh4DG278300

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002269
Physical Location & Seq
Forward (+)
49864328 .. 49866321
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG278300.1

Sequence Viewer

Length: 1422 bp
ATGGCTGGCACCGGATGGATTATTCAAGTTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAGAAGCATCACTGGAATAAGGAGTCAATCTACAAATTACCAGCCAGCCTCACAGACATGAACAAGAAGGCCTACAAGCCTCAAATAGTCTCCTTCGGGCCTTACCATTATGATCCTTCAAATCCAATGGAGGAGCACAAGCATTGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGTACGTTGAGTTGTTTGTAGATGCATTGGCTGAAGTAGAGAATGATTTGAAGGACTCATATACTTTGCTTCATTCTGTGCCGAAAGAAGTTACAGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTGGAAATCCTAAGGACTGCTGCTCATGTACTGGACGATTATGCTCCTAATGATCCCATCTTCGGCAATCATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAGCATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTCATACTCAAGTTCCTCTGCTCCTGCAATGTTTCTCCAAACATGGGAAAGTGTGTGCATGTATTGGACGTGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCACAAATATATTCGCCACAATATAAGGTTAAGGGAGCGTGACGATGAGATCATCTGGTCTGCAACGGCGCTCAATGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCACCCGGGGAGTGCTGAGGCTCCCTGTTGTTGTTGTGGATGACACCACCGAGTCGATGTTCTTGAATCTGATGGCATTTGAAAGATTCCATGTAGGAGCAGGAAATGAGGTGACTTCTTATTTAATCTTCATGGACAACATCATAGACAATGCTAGGGATGTCTCCCTCTTACACTCGAAGGGGATCATCCATAATAGTCTGGGGAGTGACAAAGCAGTGGCTAATCTGTTCAATTCACTATCTAAGGATGTGACACAAGACCCGGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATAGGTGGCGTGCCAATCTTATTCACACTTACTTCAGAAATCCATGGGCTGTTATCTCTTTCGTTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATTCCATATATCCCTATTACTTTCCACCAAATGATTCTTCTCCTTCCACTGTCATTTTTGTTCCGGCAGCTCCTCCTCCTCCAAATCATTCATCATCCGACTCCACCTGCCCATCAACACTAGTGATTTCTTTCATTCTTGTTTCCATGGTATGCATGCTTACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

473

Amino Acids

53.92

Weight (kDa)

6.62

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 410 1.5e-114 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1367
Acc36I ACCTGC 1 cut(s) 1367
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 1 cut(s) 215
AclWI GGATC 3 cut(s) 173, 428, 1013
AcuI CTGAAG 3 cut(s) 297, 659, 1162
AfaI GTAC 3 cut(s) 248, 411, 659
AfiI CCNNNNNNNGG 2 cut(s) 443, 629
AflIII ACRYGT 2 cut(s) 489, 654
AhlI ACTAGT 1 cut(s) 1372
AjiI CACGTC 1 cut(s) 655
AjuI GAANNNNNNNTTGG 2 cut(s) 1273, 1305
AluBI AGCT 5 cut(s) 535, 560, 586, 1241, 1322
AluI AGCT 5 cut(s) 535, 560, 586, 1241, 1322
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 160, 988
AlwI GGATC 3 cut(s) 173, 428, 1013
Ama87I CYCGRG 1 cut(s) 825
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 4 cut(s) 217, 401, 1092, 1319
AspLEI GCGC 3 cut(s) 215, 217, 760
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 3 cut(s) 826, 827, 1085
AsuHPI GGTGA 2 cut(s) 814, 943
AvaI CYCGRG 1 cut(s) 825
AxyI CCTNAGG 1 cut(s) 392
BanI GGYRCC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 1210
Bbv12I GWGCWC 1 cut(s) 204
BbvCI CCTCAGC 1 cut(s) 836
BbvI GCAGC 4 cut(s) 204, 388, 1104, 1331
BccI CCATC 5 cut(s) 9, 362, 446, 886, 1372
BceAI ACGGC 2 cut(s) 771, 1123
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 3 cut(s) 826, 827, 1085
BcoDI GTCTC 2 cut(s) 160, 988
BcuI ACTAGT 1 cut(s) 1372
BfaI CTAG 2 cut(s) 975, 1373
BfmI CTRYAG 2 cut(s) 1129, 1236
BfoI RGCGCY 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 1367
BglII AGATCT 1 cut(s) 37
BisI GCNGC 4 cut(s) 218, 402, 1093, 1320
BlsI GCNGC 4 cut(s) 219, 403, 1094, 1321
Bme1390I CCNGG 3 cut(s) 826, 827, 1085
BmeT110I CYCGRG 1 cut(s) 825
BmgBI CACGTC 1 cut(s) 655
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 3 cut(s) 10, 461, 842
BmrFI CCNGG 3 cut(s) 826, 827, 1085
BmsI GCATC 2 cut(s) 82, 256
BpiI GAAGAC 1 cut(s) 1210
Bpu10I CCTNAGC 2 cut(s) 561, 836
BpuEI CTTGAG 3 cut(s) 26, 521, 578
BpuMI CCSGG 3 cut(s) 826, 827, 1085
BsaJI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1398
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 629
Bse1I ACTGG 3 cut(s) 83, 417, 1252
Bse21I CCTNAGG 1 cut(s) 392
Bse3DI GCAATG 2 cut(s) 208, 619
BseDI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1398
BseGI GGATG 7 cut(s) 20, 571, 865, 985, 1008, 1075, 1346
BseLI CCNNNNNNNGG 2 cut(s) 443, 629
BseMI GCAATG 2 cut(s) 208, 619
BseMII CTCAG 2 cut(s) 827, 1256
BseNI ACTGG 3 cut(s) 83, 417, 1252
BsePI GCGCGC 1 cut(s) 213
BseRI GAGGAG 4 cut(s) 212, 1314, 1317, 1320
BseXI GCAGC 4 cut(s) 204, 388, 1104, 1331
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 825
BsiSI CCGG 4 cut(s) 12, 826, 1085, 1316
BslI CCNNNNNNNGG 2 cut(s) 443, 629
BsmAI GTCTC 2 cut(s) 160, 988
BsnI GGCC 2 cut(s) 137, 166
BsoBI CYCGRG 1 cut(s) 825
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 6 cut(s) 37, 178, 433, 688, 738, 1005
Bsp19I CCATGG 2 cut(s) 1187, 1398
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 2 cut(s) 828, 1255
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 3 cut(s) 10, 461, 842
BspMAI CTGCAG 1 cut(s) 1133
BspMI ACCTGC 1 cut(s) 1367
BspPI GGATC 3 cut(s) 173, 428, 1013
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 2 cut(s) 208, 619
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 3 cut(s) 83, 417, 1252
BssECI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1398
BssHII GCGCGC 1 cut(s) 213
BssMI GATC 6 cut(s) 37, 178, 433, 688, 738, 1005
BssT1I CCWWGG 2 cut(s) 1187, 1398
Bst4CI ACNGT 1 cut(s) 1303
Bst6I CTCTTC 1 cut(s) 659
BstAUI TGTACA 1 cut(s) 657
BstC8I GCNNGC 5 cut(s) 7, 112, 215, 1155, 1409
BstDEI CTNAG 5 cut(s) 392, 561, 836, 1065, 1242
BstDSI CCRYGG 3 cut(s) 1139, 1187, 1398
BstF5I GGATG 7 cut(s) 20, 571, 865, 985, 1008, 1075, 1346
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 1 cut(s) 761
BstHHI GCGC 3 cut(s) 215, 217, 760
BstKTI GATC 6 cut(s) 40, 181, 436, 691, 741, 1008
BstMAI GTCTC 2 cut(s) 160, 988
BstMBI GATC 6 cut(s) 37, 178, 433, 688, 738, 1005
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNSI RCATGY 3 cut(s) 493, 647, 1411
BstSCI CCNGG 3 cut(s) 824, 825, 1083
BstSFI CTRYAG 2 cut(s) 1129, 1236
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 204, 388, 1104, 1331
BstV2I GAAGAC 1 cut(s) 1210
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
Bsu36I CCTNAGG 1 cut(s) 392
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 3 cut(s) 1139, 1187, 1398
BtrI CACGTC 1 cut(s) 655
BtsCI GGATG 7 cut(s) 20, 571, 865, 985, 1008, 1075, 1346
BtsI GCAGTG 1 cut(s) 1044
BtsIMutI CAGTG 3 cut(s) 76, 1044, 1299
BveI ACCTGC 1 cut(s) 1367
Cac8I GCNNGC 5 cut(s) 7, 112, 215, 1155, 1409
CfoI GCGC 3 cut(s) 215, 217, 760
Cfr13I GGNCC 1 cut(s) 164
Cfr9I CCCGGG 1 cut(s) 825
Csp6I GTAC 3 cut(s) 247, 410, 658
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 3 cut(s) 247, 410, 658
DdeI CTNAG 5 cut(s) 392, 561, 836, 1065, 1242
DpnI GATC 6 cut(s) 39, 180, 435, 690, 740, 1007
DpnII GATC 6 cut(s) 37, 178, 433, 688, 738, 1005
Eam1104I CTCTTC 1 cut(s) 659
EarI CTCTTC 1 cut(s) 659
Eco130I CCWWGG 2 cut(s) 1187, 1398
Eco147I AGGCCT 1 cut(s) 137
Eco57I CTGAAG 3 cut(s) 297, 659, 1162
Eco81I CCTNAGG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 825
EcoT14I CCWWGG 2 cut(s) 1187, 1398
EcoT22I ATGCAT 2 cut(s) 271, 1409
ErhI CCWWGG 2 cut(s) 1187, 1398
FbaI TGATCA 1 cut(s) 688
Fnu4HI GCNGC 4 cut(s) 218, 402, 1093, 1320
FokI GGATG 7 cut(s) 27, 578, 872, 992, 995, 1082, 1333
Fsp4HI GCNGC 4 cut(s) 218, 402, 1093, 1320
FspBI CTAG 2 cut(s) 975, 1373
GlaI GCGC 3 cut(s) 214, 216, 759
GluI GCNGC 4 cut(s) 218, 402, 1093, 1320
HaeII RGCGCY 1 cut(s) 761
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 4 cut(s) 12, 826, 1085, 1316
HhaI GCGC 3 cut(s) 215, 217, 760
Hin6I GCGC 3 cut(s) 213, 215, 758
HinP1I GCGC 3 cut(s) 213, 215, 758
HpaII CCGG 4 cut(s) 12, 826, 1085, 1316
HphI GGTGA 2 cut(s) 814, 943
Hpy166II GTNNAC 2 cut(s) 658, 1233
Hpy188I TCNGA 4 cut(s) 891, 1181, 1245, 1351
Hpy188III TCNNGA 4 cut(s) 43, 527, 784, 883
Hpy8I GTNNAC 2 cut(s) 658, 1233
HpyAV CCTTC 9 cut(s) 47, 127, 169, 192, 289, 563, 994, 1106, 1305
HpyCH4III ACNGT 1 cut(s) 1303
HpyCH4IV ACGT 2 cut(s) 249, 654
HpyCH4V TGCA 9 cut(s) 269, 352, 612, 643, 752, 794, 1108, 1131, 1407
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 5 cut(s) 392, 561, 836, 1065, 1242
HpySE526I ACGT 2 cut(s) 249, 654
HspAI GCGC 3 cut(s) 213, 215, 758
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 6 cut(s) 37, 178, 433, 688, 738, 1005
LmnI GCTCC 8 cut(s) 199, 430, 465, 611, 724, 846, 917, 1327
Lsp1109I GCAGC 4 cut(s) 204, 388, 1104, 1331
LweI GCATC 2 cut(s) 82, 256
MaeI CTAG 2 cut(s) 975, 1373
MaeII ACGT 2 cut(s) 249, 654
MaeIII GTNAC 7 cut(s) 334, 485, 577, 728, 931, 1028, 1072
MalI GATC 6 cut(s) 39, 180, 435, 690, 740, 1007
MboI GATC 6 cut(s) 37, 178, 433, 688, 738, 1005
MboII GAAGA 6 cut(s) 47, 433, 676, 940, 1210, 1281
MfeI CAATTG 1 cut(s) 1417
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 4 cut(s) 101, 1054, 1101, 1417
MlyI GAGTC 5 cut(s) 98, 293, 676, 881, 1346
MmeI TCCRAC 1 cut(s) 1374
Mph1103I ATGCAT 2 cut(s) 271, 1409
MseI TTAA 4 cut(s) 30, 494, 719, 944
MslI CAYNNNNRTG 2 cut(s) 122, 494
MspI CCGG 4 cut(s) 12, 826, 1085, 1316
MspR9I CCNGG 3 cut(s) 826, 827, 1085
MunI CAATTG 1 cut(s) 1417
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 3 cut(s) 826, 827, 1085
NcoI CCATGG 2 cut(s) 1187, 1398
NdeII GATC 6 cut(s) 37, 178, 433, 688, 738, 1005
NlaIV GGNNCC 3 cut(s) 10, 461, 842
NmuCI GTSAC 6 cut(s) 485, 577, 728, 931, 1028, 1072
NsiI ATGCAT 2 cut(s) 271, 1409
NspI RCATGY 3 cut(s) 493, 647, 1411
PaeI GCATGC 1 cut(s) 1411
PaqCI CACCTGC 1 cut(s) 1367
PauI GCGCGC 1 cut(s) 213
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 489
PfeI GAWTC 5 cut(s) 505, 774, 886, 906, 1286
PkrI GCNGC 4 cut(s) 219, 403, 1094, 1321
PleI GAGTC 5 cut(s) 97, 293, 675, 880, 1346
PpsI GAGTC 5 cut(s) 97, 293, 675, 880, 1346
PscI ACATGT 1 cut(s) 489
PspN4I GGNNCC 3 cut(s) 10, 461, 842
PspPI GGNCC 1 cut(s) 164
PstI CTGCAG 1 cut(s) 1133
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 1 cut(s) 213
RsaI GTAC 3 cut(s) 248, 411, 659
RsaNI GTAC 3 cut(s) 247, 410, 658
RseI CAYNNNNRTG 2 cut(s) 122, 494
SaqAI TTAA 4 cut(s) 30, 494, 719, 944
SatI GCNGC 4 cut(s) 218, 402, 1093, 1320
Sau3AI GATC 6 cut(s) 37, 178, 433, 688, 738, 1005
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 5 cut(s) 98, 293, 676, 881, 1346
ScrFI CCNGG 3 cut(s) 826, 827, 1085
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 2 cut(s) 82, 256
SfcI CTRYAG 2 cut(s) 1129, 1236
SmaI CCCGGG 1 cut(s) 827
SmiMI CAYNNNNRTG 2 cut(s) 122, 494
SmlI CTYRAG 3 cut(s) 41, 500, 593
SmoI CTYRAG 3 cut(s) 41, 500, 593
SpeI ACTAGT 1 cut(s) 1372
SphI GCATGC 1 cut(s) 1411
Sse9I AATT 4 cut(s) 101, 1054, 1101, 1417
SseBI AGGCCT 1 cut(s) 137
SspMI CTAG 2 cut(s) 975, 1373
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 3 cut(s) 824, 825, 1083
StyI CCWWGG 2 cut(s) 1187, 1398
TaaI ACNGT 1 cut(s) 1303
TaiI ACGT 2 cut(s) 252, 657
TaqI TCGA 2 cut(s) 875, 998
TasI AATT 4 cut(s) 101, 1054, 1101, 1417
TatI WGTACW 2 cut(s) 409, 657
TfiI GAWTC 5 cut(s) 505, 774, 886, 906, 1286
Tru1I TTAA 4 cut(s) 30, 494, 719, 944
Tru9I TTAA 4 cut(s) 30, 494, 719, 944
TscAI CASTG 3 cut(s) 83, 1044, 1306
TseFI GTSAC 6 cut(s) 485, 577, 728, 931, 1028, 1072
TseI GCWGC 4 cut(s) 217, 401, 1092, 1319
Tsp45I GTSAC 6 cut(s) 485, 577, 728, 931, 1028, 1072
TspMI CCCGGG 1 cut(s) 825
TspRI CASTG 3 cut(s) 83, 1044, 1306
XceI RCATGY 3 cut(s) 493, 647, 1411
XmaI CCCGGG 1 cut(s) 825
XspI CTAG 2 cut(s) 975, 1373
Zsp2I ATGCAT 2 cut(s) 271, 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.