Rh4BG281000

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002269
Physical Location & Seq
Forward (+)
45484600 .. 45486848
2249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG281000.1

Sequence Viewer

Length: 792 bp
ATGGGAAAGTGTGTGCATGTATTGGATGTGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCACAAATATATTCGCCACAAAATAAGGTCAATGGAGCGTGATGATGAGATTATCTGGTCTGCAACAGAGCTCAACGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCGCCCGGGGAGTGCTGAGGCTCCCTGTTGTTCTTGTGGATGACACCACTGAGTCCATGTTTTTAAATCTGATGGCATTTGAGAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCTAGGGATGTCGCCCTCTTACATTCGAAAGGGATCATCCAGAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTGTTCAATTCACTCTCTAATGATGTGACACAGGACCCAGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATAGGTGGCGCGCCAATCTTATTCACACTTACTTCAGAAATCCATGGGCTGTTATCTCTTTCGTTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATTCCATATATCCCTATTACCGTCCACCAAATGATTCTTCTCCTTCCACTGTCATTCTCGTTCCGGCTCCTCCTCCTCCAAATCATTCATCATCCGACTCCACCTCCCCATCAACACTTGTGATTTCTTTCATCCTTGTTACCATCGGATGCTTGCTTACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

263

Amino Acids

29.74

Weight (kDa)

8.38

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 3 - 201 2.8e-67 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 528
AclWI GGATC 1 cut(s) 386
AcuI CTGAAG 2 cut(s) 32, 535
AfaI GTAC 1 cut(s) 32
AgsI TTSAA 3 cut(s) 157, 427, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 646, 678
AluBI AGCT 2 cut(s) 133, 614
AluI AGCT 2 cut(s) 133, 614
Alw21I GWGCWC 1 cut(s) 135
AlwI GGATC 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 198
ApeKI GCWGC 1 cut(s) 465
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AscI GGCGCGCC 1 cut(s) 526
AspLEI GCGC 2 cut(s) 528, 530
AspS9I GGNCC 1 cut(s) 454
AsuC2I CCSGG 2 cut(s) 199, 200
AsuHPI GGTGA 1 cut(s) 316
AsuII TTCGAA 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 198
AvaII GGWCC 1 cut(s) 454
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 583
Bbv12I GWGCWC 1 cut(s) 135
BbvCI CCTCAGC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 477
BccI CCATC 3 cut(s) 259, 742, 776
BceAI ACGGC 1 cut(s) 496
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 2 cut(s) 199, 200
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 2 cut(s) 502, 609
BisI GCNGC 1 cut(s) 466
BlsI GCNGC 1 cut(s) 467
Bme1390I CCNGG 2 cut(s) 199, 200
Bme18I GGWCC 1 cut(s) 454
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 3 cut(s) 215, 456, 693
BmrFI CCNGG 2 cut(s) 199, 200
BmsI GCATC 1 cut(s) 764
BpiI GAAGAC 1 cut(s) 583
Bpu10I CCTNAGC 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 371
BpuMI CCSGG 2 cut(s) 199, 200
BsaJI CCNNGG 4 cut(s) 198, 199, 512, 560
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bse1I ACTGG 1 cut(s) 625
BseDI CCNNGG 4 cut(s) 198, 199, 512, 560
BseGI GGATG 7 cut(s) 31, 238, 358, 381, 716, 756, 779
BseMII CTCAG 3 cut(s) 200, 234, 629
BseNI ACTGG 1 cut(s) 625
BsePI GCGCGC 1 cut(s) 526
BseRI GAGGAG 3 cut(s) 684, 687, 690
BseXI GCAGC 1 cut(s) 477
Bsh1236I CGCG 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 2 cut(s) 199, 689
BsmI GAATGC 1 cut(s) 394
BsoBI CYCGRG 1 cut(s) 198
Bsp119I TTCGAA 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 135
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 2 cut(s) 61, 378
Bsp19I CCATGG 1 cut(s) 560
BspCNI CTCAG 3 cut(s) 201, 235, 628
BspFNI CGCG 1 cut(s) 528
BspLI GGNNCC 3 cut(s) 215, 456, 693
BspMAI CTGCAG 1 cut(s) 506
BspPI GGATC 1 cut(s) 386
BspT104I TTCGAA 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 1 cut(s) 625
BssECI CCNNGG 4 cut(s) 198, 199, 512, 560
BssHII GCGCGC 1 cut(s) 526
BssMI GATC 2 cut(s) 61, 378
BssT1I CCWWGG 1 cut(s) 560
Bst4CI ACNGT 2 cut(s) 647, 676
Bst6I CTCTTC 2 cut(s) 32, 326
BstAUI TGTACA 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 371
BstC8I GCNNGC 2 cut(s) 528, 779
BstDEI CTNAG 3 cut(s) 209, 243, 615
BstDSI CCRYGG 2 cut(s) 512, 560
BstF5I GGATG 7 cut(s) 31, 238, 358, 381, 716, 756, 779
BstFNI CGCG 1 cut(s) 528
BstHHI GCGC 2 cut(s) 528, 530
BstKTI GATC 2 cut(s) 64, 381
BstMBI GATC 2 cut(s) 61, 378
BstNSI RCATGY 1 cut(s) 20
BstSCI CCNGG 2 cut(s) 197, 198
BstSFI CTRYAG 2 cut(s) 502, 609
BstUI CGCG 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 583
BtgI CCRYGG 2 cut(s) 512, 560
BtsCI GGATG 7 cut(s) 31, 238, 358, 381, 716, 756, 779
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 3 cut(s) 240, 417, 672
Cac8I GCNNGC 2 cut(s) 528, 779
CfoI GCGC 2 cut(s) 528, 530
Cfr13I GGNCC 1 cut(s) 454
Cfr9I CCCGGG 1 cut(s) 198
Csp6I GTAC 1 cut(s) 31
CviAII CATG 6 cut(s) 17, 53, 250, 284, 325, 561
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 3 cut(s) 209, 243, 615
DpnI GATC 2 cut(s) 63, 380
DpnII GATC 2 cut(s) 61, 378
DraI TTTAAA 1 cut(s) 258
Eam1104I CTCTTC 2 cut(s) 32, 326
EarI CTCTTC 2 cut(s) 32, 326
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 560
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 32, 535
Eco88I CYCGRG 1 cut(s) 198
EcoICRI GAGCTC 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 454
EcoT14I CCWWGG 1 cut(s) 560
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 560
FaeI CATG 6 cut(s) 20, 56, 253, 287, 328, 564
FatI CATG 6 cut(s) 16, 52, 249, 283, 324, 560
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 466
FokI GGATG 7 cut(s) 38, 245, 365, 368, 703, 743, 786
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 466
FspBI CTAG 1 cut(s) 348
GlaI GCGC 2 cut(s) 527, 529
GluI GCNGC 1 cut(s) 466
HapII CCGG 2 cut(s) 199, 689
HhaI GCGC 2 cut(s) 528, 530
Hin1II CATG 6 cut(s) 20, 56, 253, 287, 328, 564
Hin6I GCGC 2 cut(s) 526, 528
HinP1I GCGC 2 cut(s) 526, 528
HinfI GANTC 6 cut(s) 40, 147, 245, 279, 659, 722
HpaII CCGG 2 cut(s) 199, 689
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 3 cut(s) 31, 606, 650
Hpy188I TCNGA 5 cut(s) 264, 554, 618, 721, 773
Hpy188III TCNNGA 2 cut(s) 157, 385
Hpy8I GTNNAC 3 cut(s) 31, 606, 650
HpyAV CCTTC 2 cut(s) 479, 678
HpyCH4III ACNGT 2 cut(s) 647, 676
HpyCH4V TGCA 5 cut(s) 16, 125, 167, 481, 504
HpyF3I CTNAG 3 cut(s) 209, 243, 615
Hsp92II CATG 6 cut(s) 20, 56, 253, 287, 328, 564
HspAI GCGC 2 cut(s) 526, 528
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 2 cut(s) 61, 378
LmnI GCTCC 4 cut(s) 97, 219, 290, 697
Lsp1109I GCAGC 1 cut(s) 477
LweI GCATC 1 cut(s) 764
MaeI CTAG 1 cut(s) 348
MaeIII GTNAC 4 cut(s) 304, 401, 445, 763
MalI GATC 2 cut(s) 63, 380
MboI GATC 2 cut(s) 61, 378
MboII GAAGA 4 cut(s) 49, 313, 583, 654
MfeI CAATTG 1 cut(s) 787
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 3 cut(s) 427, 474, 787
MlyI GAGTC 3 cut(s) 49, 254, 716
MmeI TCCRAC 1 cut(s) 744
MseI TTAA 1 cut(s) 257
MspI CCGG 2 cut(s) 199, 689
MspR9I CCNGG 2 cut(s) 199, 200
MunI CAATTG 1 cut(s) 787
Mva1269I GAATGC 1 cut(s) 394
MvnI CGCG 1 cut(s) 528
NciI CCSGG 2 cut(s) 199, 200
NcoI CCATGG 1 cut(s) 560
NdeII GATC 2 cut(s) 61, 378
NlaIII CATG 6 cut(s) 20, 56, 253, 287, 328, 564
NlaIV GGNNCC 3 cut(s) 215, 456, 693
NmuCI GTSAC 3 cut(s) 304, 401, 445
NspI RCATGY 1 cut(s) 20
NspV TTCGAA 1 cut(s) 371
PalAI GGCGCGCC 1 cut(s) 526
PauI GCGCGC 1 cut(s) 526
PctI GAATGC 1 cut(s) 394
PfeI GAWTC 3 cut(s) 147, 279, 659
PkrI GCNGC 1 cut(s) 467
PleI GAGTC 3 cut(s) 48, 253, 716
PpsI GAGTC 3 cut(s) 48, 253, 716
PpuMI RGGWCCY 1 cut(s) 454
Psp124BI GAGCTC 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 454
PspN4I GGNNCC 3 cut(s) 215, 456, 693
PspPI GGNCC 1 cut(s) 454
PspPPI RGGWCCY 1 cut(s) 454
PstI CTGCAG 1 cut(s) 506
PteI GCGCGC 1 cut(s) 526
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 1 cut(s) 466
Sau3AI GATC 2 cut(s) 61, 378
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 3 cut(s) 49, 254, 716
ScrFI CCNGG 2 cut(s) 199, 200
SduI GDGCHC 1 cut(s) 135
SetI ASST 7 cut(s) 92, 135, 155, 306, 524, 616, 731
SfaNI GCATC 1 cut(s) 764
SfcI CTRYAG 2 cut(s) 502, 609
SfuI TTCGAA 1 cut(s) 371
SgsI GGCGCGCC 1 cut(s) 526
SinI GGWCC 1 cut(s) 454
SmaI CCCGGG 1 cut(s) 200
Sse9I AATT 3 cut(s) 427, 474, 787
SspMI CTAG 1 cut(s) 348
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 2 cut(s) 197, 198
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 2 cut(s) 647, 676
TaqI TCGA 1 cut(s) 371
TasI AATT 3 cut(s) 427, 474, 787
TatI WGTACW 1 cut(s) 30
TfiI GAWTC 3 cut(s) 147, 279, 659
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TscAI CASTG 3 cut(s) 247, 417, 679
TseFI GTSAC 3 cut(s) 304, 401, 445
TseI GCWGC 1 cut(s) 465
Tsp45I GTSAC 3 cut(s) 304, 401, 445
TspDTI ATGAA 3 cut(s) 313, 702, 745
TspMI CCCGGG 1 cut(s) 198
TspRI CASTG 3 cut(s) 247, 417, 679
VpaK11BI GGWCC 1 cut(s) 454
XceI RCATGY 1 cut(s) 20
XmaI CCCGGG 1 cut(s) 198
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.