Rroxscaffold_5G00369200

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002269
Physical Location & Seq
Forward (+)
51045244 .. 51047165
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369200.1

Sequence Viewer

Length: 1419 bp
ATGGCTGGCACCGGATGGATTATTCAAGTTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAGAAGCATCACTGGAATAAGGGGTCAATCTACAAATTACCAGCCAGCCTCACAGACATGAACAAGAAGGCCTACAAGCCTCAGACAGTCTCCTTCGGGCCTTACCATTATGATCCTTCAAATCCAATGGAGGAGCACAAGCATCGCACGCTGCTTCATTTCCTCAAAAGATGTGGAAAGTCTGTTGAGCTGTTTGTAGATGCATTGGCTGAAGTAAAGAATGATTTGAAGGACTCATATACTCTGCTTCATTCTGTGCCGAAAGAAGTTACAGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTAGAAATTTTAAGGACTGCTGCTCATGTACTGGAAGATTATGCTCCTAATGATCCCATCTTCGGCAATCATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTAGCAGTTGAACATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTCATACTCAAGTTCCTCTGCTCCTGCAATGTTTCTCCAAACATGGGAAAGTGTGTGCATGTATTGGATGTCTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCACAAATATATTCGCCACAAAATAAGGTCAATGGAGCGTGATGATGAGATTATCTGGTCCGCAACAGAGCTCAACGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTGCAAGCCTCAAAGACATCACATTCGCCCGGGGAGTGCTGAGGCTCCCTGTTGTTCTTGTGGATGACACCACTGAGTCCATGTTTTTAAATCTTATGGCATTTGAGAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCTAGGGATGTCGCCCTCTTACACTCGAAAGGGATCATCCATAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTGTTCAATTCACTCTCTAAGGATGTGACACAAGACCCGGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATAGGTGGCGCGCCAATCTTATTCACACTTACTTCAGAAATCCATGGGCTGTTATCTCTTTCGTTGCTGCTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATTCCATATATCCCTATTACCGTCCACCAAATGATCCTTCTCCTTCCACTGTCATTCTCGTTCCTGCTCCTCCTCCTCCAAACCATTCATCCTCCGACTCCACCGCCCCATCAACACTAGTGATTTCTTTCATTCTTGTTTCCATGTTATGCATGCTTACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

472

Amino Acids

53.71

Weight (kDa)

7.35

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 410 1.9e-115 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 657
AccII CGCG 1 cut(s) 1155
AciI CCGC 2 cut(s) 750, 1356
AclWI GGATC 4 cut(s) 173, 428, 1013, 1280
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 3 cut(s) 297, 659, 1162
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 2 cut(s) 443, 629
AflIII ACRYGT 1 cut(s) 489
AgsI TTSAA 9 cut(s) 26, 63, 186, 295, 530, 548, 784, 1054, 1100
AhlI ACTAGT 1 cut(s) 1369
AjuI GAANNNNNNNTTGG 2 cut(s) 1273, 1305
AluBI AGCT 6 cut(s) 256, 535, 560, 586, 760, 1241
AluI AGCT 6 cut(s) 256, 535, 560, 586, 760, 1241
Alw21I GWGCWC 2 cut(s) 204, 762
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 4 cut(s) 173, 428, 1013, 1280
Ama87I CYCGRG 1 cut(s) 825
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 4 cut(s) 217, 401, 1092, 1211
ApoI RAATTY 1 cut(s) 387
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AscI GGCGCGCC 1 cut(s) 1153
AspLEI GCGC 2 cut(s) 1155, 1157
AspS9I GGNCC 2 cut(s) 164, 747
AsuC2I CCSGG 3 cut(s) 826, 827, 1085
AsuHPI GGTGA 1 cut(s) 943
AvaI CYCGRG 1 cut(s) 825
AvaII GGWCC 1 cut(s) 747
BanI GGYRCC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 762
BbsI GAAGAC 1 cut(s) 1210
Bbv12I GWGCWC 2 cut(s) 204, 762
BbvCI CCTCAGC 1 cut(s) 836
BbvI GCAGC 4 cut(s) 204, 388, 1104, 1198
BccI CCATC 4 cut(s) 9, 362, 446, 1369
BceAI ACGGC 1 cut(s) 1123
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 3 cut(s) 826, 827, 1085
BcoDI GTCTC 1 cut(s) 160
BcuI ACTAGT 1 cut(s) 1369
BfaI CTAG 3 cut(s) 383, 975, 1370
BfmI CTRYAG 3 cut(s) 658, 1129, 1236
BglII AGATCT 1 cut(s) 37
BisI GCNGC 4 cut(s) 218, 402, 1093, 1212
BlsI GCNGC 4 cut(s) 219, 403, 1094, 1213
Bme1390I CCNGG 3 cut(s) 826, 827, 1085
Bme18I GGWCC 1 cut(s) 747
BmeT110I CYCGRG 1 cut(s) 825
BmgT120I GGNCC 2 cut(s) 164, 747
BmiI GGNNCC 3 cut(s) 10, 461, 842
BmrFI CCNGG 3 cut(s) 826, 827, 1085
BmsI GCATC 3 cut(s) 82, 217, 256
BpiI GAAGAC 1 cut(s) 1210
Bpu10I CCTNAGC 2 cut(s) 561, 836
BpuEI CTTGAG 3 cut(s) 26, 521, 578
BpuMI CCSGG 3 cut(s) 826, 827, 1085
BsaJI CCNNGG 4 cut(s) 825, 826, 1139, 1187
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 629
Bse1I ACTGG 3 cut(s) 83, 417, 1252
Bse3DI GCAATG 1 cut(s) 619
BseDI CCNNGG 4 cut(s) 825, 826, 1139, 1187
BseGI GGATG 8 cut(s) 20, 571, 658, 865, 985, 1008, 1075, 1340
BseLI CCNNNNNNNGG 2 cut(s) 443, 629
BseMI GCAATG 1 cut(s) 619
BseMII CTCAG 4 cut(s) 161, 827, 861, 1256
BseNI ACTGG 3 cut(s) 83, 417, 1252
BsePI GCGCGC 1 cut(s) 1153
BseRI GAGGAG 4 cut(s) 212, 1311, 1314, 1317
BseXI GCAGC 4 cut(s) 204, 388, 1104, 1198
Bsh1236I CGCG 1 cut(s) 1155
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 204, 762
BsiHKCI CYCGRG 1 cut(s) 825
BsiSI CCGG 3 cut(s) 12, 826, 1085
BslI CCNNNNNNNGG 2 cut(s) 443, 629
BsmAI GTCTC 1 cut(s) 160
BsnI GGCC 2 cut(s) 137, 166
BsoBI CYCGRG 1 cut(s) 825
Bsp1286I GDGCHC 2 cut(s) 204, 762
Bsp143I GATC 6 cut(s) 37, 178, 433, 688, 1005, 1285
Bsp19I CCATGG 1 cut(s) 1187
BspACI CCGC 2 cut(s) 750, 1356
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 4 cut(s) 160, 828, 862, 1255
BspFNI CGCG 1 cut(s) 1155
BspLI GGNNCC 3 cut(s) 10, 461, 842
BspMAI CTGCAG 1 cut(s) 1133
BspPI GGATC 4 cut(s) 173, 428, 1013, 1280
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 619
BsrI ACTGG 3 cut(s) 83, 417, 1252
BssECI CCNNGG 4 cut(s) 825, 826, 1139, 1187
BssHII GCGCGC 1 cut(s) 1153
BssMI GATC 6 cut(s) 37, 178, 433, 688, 1005, 1285
BssT1I CCWWGG 1 cut(s) 1187
Bst4CI ACNGT 3 cut(s) 154, 1274, 1303
Bst6I CTCTTC 2 cut(s) 659, 953
BstC8I GCNNGC 6 cut(s) 7, 112, 215, 802, 1155, 1406
BstDEI CTNAG 6 cut(s) 147, 561, 836, 870, 1065, 1242
BstDSI CCRYGG 2 cut(s) 1139, 1187
BstF5I GGATG 8 cut(s) 20, 571, 658, 865, 985, 1008, 1075, 1340
BstFNI CGCG 1 cut(s) 1155
BstHHI GCGC 2 cut(s) 1155, 1157
BstKTI GATC 6 cut(s) 40, 181, 436, 691, 1008, 1288
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 6 cut(s) 37, 178, 433, 688, 1005, 1285
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNSI RCATGY 3 cut(s) 493, 647, 1408
BstSCI CCNGG 3 cut(s) 824, 825, 1083
BstSFI CTRYAG 3 cut(s) 658, 1129, 1236
BstUI CGCG 1 cut(s) 1155
BstV1I GCAGC 4 cut(s) 204, 388, 1104, 1198
BstV2I GAAGAC 1 cut(s) 1210
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 2 cut(s) 1139, 1187
BtgZI GCGATG 1 cut(s) 194
BtsCI GGATG 8 cut(s) 20, 571, 658, 865, 985, 1008, 1075, 1340
BtsI GCAGTG 1 cut(s) 1044
BtsIMutI CAGTG 4 cut(s) 76, 867, 1044, 1299
Cac8I GCNNGC 6 cut(s) 7, 112, 215, 802, 1155, 1406
CfoI GCGC 2 cut(s) 1155, 1157
Cfr13I GGNCC 2 cut(s) 164, 747
Cfr9I CCCGGG 1 cut(s) 825
Csp6I GTAC 1 cut(s) 410
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 6 cut(s) 147, 561, 836, 870, 1065, 1242
DpnI GATC 6 cut(s) 39, 180, 435, 690, 1007, 1287
DpnII GATC 6 cut(s) 37, 178, 433, 688, 1005, 1285
DraI TTTAAA 1 cut(s) 885
Eam1104I CTCTTC 2 cut(s) 659, 953
EarI CTCTTC 2 cut(s) 659, 953
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 1 cut(s) 1187
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 762
Eco47I GGWCC 1 cut(s) 747
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 3 cut(s) 297, 659, 1162
Eco88I CYCGRG 1 cut(s) 825
EcoICRI GAGCTC 1 cut(s) 760
EcoT14I CCWWGG 1 cut(s) 1187
EcoT22I ATGCAT 2 cut(s) 271, 1406
EcoT38I GRGCYC 1 cut(s) 762
ErhI CCWWGG 1 cut(s) 1187
FbaI TGATCA 1 cut(s) 688
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 4 cut(s) 218, 402, 1093, 1212
FokI GGATG 8 cut(s) 27, 578, 665, 872, 992, 995, 1082, 1327
FriOI GRGCYC 1 cut(s) 762
Fsp4HI GCNGC 4 cut(s) 218, 402, 1093, 1212
FspBI CTAG 3 cut(s) 383, 975, 1370
GlaI GCGC 2 cut(s) 1154, 1156
GluI GCNGC 4 cut(s) 218, 402, 1093, 1212
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 3 cut(s) 12, 826, 1085
HhaI GCGC 2 cut(s) 1155, 1157
Hin6I GCGC 2 cut(s) 1153, 1155
HinP1I GCGC 2 cut(s) 1153, 1155
HinfI GANTC 7 cut(s) 299, 505, 667, 774, 872, 906, 1349
HpaII CCGG 3 cut(s) 12, 826, 1085
HphI GGTGA 1 cut(s) 943
Hpy166II GTNNAC 3 cut(s) 658, 1233, 1277
Hpy188I TCNGA 4 cut(s) 150, 1181, 1245, 1348
Hpy188III TCNNGA 3 cut(s) 43, 527, 784
Hpy8I GTNNAC 3 cut(s) 658, 1233, 1277
HpyAV CCTTC 9 cut(s) 47, 127, 169, 192, 289, 563, 1106, 1299, 1305
HpyCH4III ACNGT 3 cut(s) 154, 1274, 1303
HpyCH4V TGCA 9 cut(s) 269, 352, 612, 643, 794, 800, 1108, 1131, 1404
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 6 cut(s) 147, 561, 836, 870, 1065, 1242
HspAI GCGC 2 cut(s) 1153, 1155
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 6 cut(s) 37, 178, 433, 688, 1005, 1285
LmnI GCTCC 8 cut(s) 199, 430, 465, 611, 724, 846, 917, 1324
Lsp1109I GCAGC 4 cut(s) 204, 388, 1104, 1198
LweI GCATC 3 cut(s) 82, 217, 256
MaeI CTAG 3 cut(s) 383, 975, 1370
MaeIII GTNAC 6 cut(s) 334, 485, 577, 931, 1028, 1072
MalI GATC 6 cut(s) 39, 180, 435, 690, 1007, 1287
MboI GATC 6 cut(s) 37, 178, 433, 688, 1005, 1285
MboII GAAGA 6 cut(s) 47, 428, 433, 676, 940, 1210
MfeI CAATTG 1 cut(s) 1414
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 204, 762
MluCI AATT 5 cut(s) 101, 387, 1054, 1101, 1414
MlyI GAGTC 4 cut(s) 293, 676, 881, 1343
MmeI TCCRAC 1 cut(s) 1371
Mph1103I ATGCAT 2 cut(s) 271, 1406
MseI TTAA 4 cut(s) 30, 392, 494, 884
MslI CAYNNNNRTG 2 cut(s) 122, 494
MspI CCGG 3 cut(s) 12, 826, 1085
MspR9I CCNGG 3 cut(s) 826, 827, 1085
MunI CAATTG 1 cut(s) 1414
MvnI CGCG 1 cut(s) 1155
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 3 cut(s) 826, 827, 1085
NcoI CCATGG 1 cut(s) 1187
NdeII GATC 6 cut(s) 37, 178, 433, 688, 1005, 1285
NlaIV GGNNCC 3 cut(s) 10, 461, 842
NmuCI GTSAC 5 cut(s) 485, 577, 931, 1028, 1072
NsiI ATGCAT 2 cut(s) 271, 1406
NspI RCATGY 3 cut(s) 493, 647, 1408
PaeI GCATGC 1 cut(s) 1408
PalAI GGCGCGCC 1 cut(s) 1153
PauI GCGCGC 1 cut(s) 1153
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 489
PfeI GAWTC 3 cut(s) 505, 774, 906
PkrI GCNGC 4 cut(s) 219, 403, 1094, 1213
PleI GAGTC 4 cut(s) 293, 675, 880, 1343
PpsI GAGTC 4 cut(s) 293, 675, 880, 1343
PscI ACATGT 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 762
PspN4I GGNNCC 3 cut(s) 10, 461, 842
PspPI GGNCC 2 cut(s) 164, 747
PstI CTGCAG 1 cut(s) 1133
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 1 cut(s) 1153
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 2 cut(s) 122, 494
SacI GAGCTC 1 cut(s) 762
SaqAI TTAA 4 cut(s) 30, 392, 494, 884
SatI GCNGC 4 cut(s) 218, 402, 1093, 1212
Sau3AI GATC 6 cut(s) 37, 178, 433, 688, 1005, 1285
Sau96I GGNCC 2 cut(s) 164, 747
SchI GAGTC 4 cut(s) 293, 676, 881, 1343
ScrFI CCNGG 3 cut(s) 826, 827, 1085
SduI GDGCHC 2 cut(s) 204, 762
SfaNI GCATC 3 cut(s) 82, 217, 256
SfcI CTRYAG 3 cut(s) 658, 1129, 1236
SgsI GGCGCGCC 1 cut(s) 1153
SinI GGWCC 1 cut(s) 747
SmaI CCCGGG 1 cut(s) 827
SmiMI CAYNNNNRTG 2 cut(s) 122, 494
SmlI CTYRAG 3 cut(s) 41, 500, 593
SmoI CTYRAG 3 cut(s) 41, 500, 593
SpeI ACTAGT 1 cut(s) 1369
SphI GCATGC 1 cut(s) 1408
Sse9I AATT 5 cut(s) 101, 387, 1054, 1101, 1414
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 2 cut(s) 750, 1356
SspMI CTAG 3 cut(s) 383, 975, 1370
SstI GAGCTC 1 cut(s) 762
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 3 cut(s) 824, 825, 1083
StyI CCWWGG 1 cut(s) 1187
TaaI ACNGT 3 cut(s) 154, 1274, 1303
TaqI TCGA 1 cut(s) 998
TasI AATT 5 cut(s) 101, 387, 1054, 1101, 1414
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 3 cut(s) 505, 774, 906
Tru1I TTAA 4 cut(s) 30, 392, 494, 884
Tru9I TTAA 4 cut(s) 30, 392, 494, 884
TscAI CASTG 4 cut(s) 83, 874, 1044, 1306
TseFI GTSAC 5 cut(s) 485, 577, 931, 1028, 1072
TseI GCWGC 4 cut(s) 217, 401, 1092, 1211
Tsp45I GTSAC 5 cut(s) 485, 577, 931, 1028, 1072
TspDTI ATGAA 9 cut(s) 48, 140, 212, 305, 367, 582, 940, 1329, 1372
TspMI CCCGGG 1 cut(s) 825
TspRI CASTG 4 cut(s) 83, 874, 1044, 1306
VpaK11BI GGWCC 1 cut(s) 747
XapI RAATTY 1 cut(s) 387
XceI RCATGY 3 cut(s) 493, 647, 1408
XmaI CCCGGG 1 cut(s) 825
XmiI GTMKAC 1 cut(s) 657
XspI CTAG 3 cut(s) 383, 975, 1370
Zsp2I ATGCAT 2 cut(s) 271, 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.