Rh4BG287000

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002269
Physical Location & Seq
Reverse (-)
46021954 .. 46022544
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG287000.1

Sequence Viewer

Length: 591 bp
ATGGCTGGCACCGGATGGATTATTCAAATTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAACAGGATCGCTGGAATAAGGGGTCAATCTACAAACTACCAGCTAGCCTCACAGACATGAACAGGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGGTCTTACCACTACAATTCCTTAAATCCAATGGAGGAGCACAAGCACCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGCCTGTTGAGTTGTTTGTAGATGCATTGGCTGAAGTAGAGAATGAATTGAAGGATTCATATACTCTGTTTCGTTCTGTGCCGAAAGAAATTACGGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTGGAAATCATGAGGAGTGCTGCTCATGTACTGGGTGATTATGCTCCTAATGATCCCATCTTCGGAGAACATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAACATGATAAAGCTAAGGGAAAGTATGTCAATCATCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

196

Amino Acids

22.62

Weight (kDa)

6.3

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 186 5.8e-43 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 2 cut(s) 213, 215
AciI CCGC 1 cut(s) 211
AclWI GGATC 2 cut(s) 81, 428
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 489
AgsI TTSAA 5 cut(s) 26, 63, 295, 530, 548
AluBI AGCT 3 cut(s) 110, 535, 560
AluI AGCT 3 cut(s) 110, 535, 560
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 2 cut(s) 81, 428
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 2 cut(s) 217, 401
AseI ATTAAT 1 cut(s) 30
AspLEI GCGC 2 cut(s) 215, 217
AsuHPI GGTGA 1 cut(s) 428
AsuNHI GCTAGC 1 cut(s) 110
BanI GGYRCC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 204, 388
BccI CCATC 3 cut(s) 9, 362, 446
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 111
BglII AGATCT 1 cut(s) 37
BisI GCNGC 2 cut(s) 218, 402
BlsI GCNGC 2 cut(s) 219, 403
BmiI GGNNCC 2 cut(s) 10, 461
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 1 cut(s) 256
BmtI GCTAGC 1 cut(s) 114
BmuI ACTGGG 1 cut(s) 422
BplI GAGNNNNNCTC 2 cut(s) 388, 420
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 26, 521
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 1 cut(s) 417
BseGI GGATG 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 443
BseNI ACTGG 1 cut(s) 417
BsePI GCGCGC 1 cut(s) 213
BseRI GAGGAG 2 cut(s) 212, 409
BseXI GCAGC 2 cut(s) 204, 388
Bsh1236I CGCG 2 cut(s) 213, 215
BshFI GGCC 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 160
BsnI GGCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 3 cut(s) 37, 73, 433
BspACI CCGC 1 cut(s) 211
BspANI GGCC 1 cut(s) 137
BspFNI CGCG 2 cut(s) 213, 215
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 10, 461
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 2 cut(s) 81, 428
BspT107I GGYRCC 1 cut(s) 8
BsrI ACTGG 1 cut(s) 417
BssHII GCGCGC 1 cut(s) 213
BssMI GATC 3 cut(s) 37, 73, 433
Bst4CI ACNGT 1 cut(s) 154
BstC8I GCNNGC 3 cut(s) 7, 112, 215
BstDEI CTNAG 1 cut(s) 561
BstF5I GGATG 1 cut(s) 20
BstFNI CGCG 2 cut(s) 213, 215
BstHHI GCGC 2 cut(s) 215, 217
BstKTI GATC 3 cut(s) 40, 76, 436
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 3 cut(s) 37, 73, 433
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNSI RCATGY 1 cut(s) 493
BstUI CGCG 2 cut(s) 213, 215
BstV1I GCAGC 2 cut(s) 204, 388
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 1 cut(s) 137
BtsCI GGATG 1 cut(s) 20
Cac8I GCNNGC 3 cut(s) 7, 112, 215
CciI TCATGA 1 cut(s) 390
CfoI GCGC 2 cut(s) 215, 217
Csp6I GTAC 1 cut(s) 410
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviAII CATG 9 cut(s) 49, 124, 379, 391, 407, 452, 464, 490, 551
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 1 cut(s) 561
DpnI GATC 3 cut(s) 39, 75, 435
DpnII GATC 3 cut(s) 37, 73, 433
Eco147I AGGCCT 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 297
EcoT22I ATGCAT 1 cut(s) 271
FaeI CATG 9 cut(s) 52, 127, 382, 394, 410, 455, 467, 493, 554
FatI CATG 9 cut(s) 48, 123, 378, 390, 406, 451, 463, 489, 550
Fnu4HI GCNGC 2 cut(s) 218, 402
FokI GGATG 1 cut(s) 27
Fsp4HI GCNGC 2 cut(s) 218, 402
FspBI CTAG 1 cut(s) 111
GlaI GCGC 2 cut(s) 214, 216
GluI GCNGC 2 cut(s) 218, 402
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 12
HhaI GCGC 2 cut(s) 215, 217
Hin1II CATG 9 cut(s) 52, 127, 382, 394, 410, 455, 467, 493, 554
Hin6I GCGC 2 cut(s) 213, 215
HinP1I GCGC 2 cut(s) 213, 215
HinfI GANTC 2 cut(s) 299, 505
HpaII CCGG 1 cut(s) 12
HphI GGTGA 1 cut(s) 428
Hpy188I TCNGA 1 cut(s) 446
Hpy188III TCNNGA 3 cut(s) 43, 391, 527
HpyAV CCTTC 4 cut(s) 47, 127, 169, 289
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 269, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 1 cut(s) 561
Hsp92II CATG 9 cut(s) 52, 127, 382, 394, 410, 455, 467, 493, 554
HspAI GCGC 2 cut(s) 213, 215
Kzo9I GATC 3 cut(s) 37, 73, 433
LmnI GCTCC 3 cut(s) 199, 430, 465
Lsp1109I GCAGC 2 cut(s) 204, 388
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 485
MalI GATC 3 cut(s) 39, 75, 435
MboI GATC 3 cut(s) 37, 73, 433
MboII GAAGA 2 cut(s) 47, 433
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 4 cut(s) 27, 178, 290, 333
MnlI CCTC 5 cut(s) 125, 156, 190, 239, 387
Mph1103I ATGCAT 1 cut(s) 271
MseI TTAA 3 cut(s) 30, 185, 494
MslI CAYNNNNRTG 2 cut(s) 122, 494
MspI CCGG 1 cut(s) 12
MvnI CGCG 2 cut(s) 213, 215
MwoI GCNNNNNNNGC 1 cut(s) 214
NdeII GATC 3 cut(s) 37, 73, 433
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 9 cut(s) 52, 127, 382, 394, 410, 455, 467, 493, 554
NlaIV GGNNCC 2 cut(s) 10, 461
NmuCI GTSAC 1 cut(s) 485
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 1 cut(s) 493
PagI TCATGA 1 cut(s) 390
PauI GCGCGC 1 cut(s) 213
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 489
PfeI GAWTC 2 cut(s) 299, 505
PkrI GCNGC 2 cut(s) 219, 403
PscI ACATGT 1 cut(s) 489
PshBI ATTAAT 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 10, 461
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 1 cut(s) 213
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 2 cut(s) 122, 494
SaqAI TTAA 3 cut(s) 30, 185, 494
SatI GCNGC 2 cut(s) 218, 402
Sau3AI GATC 3 cut(s) 37, 73, 433
SduI GDGCHC 1 cut(s) 204
SetI ASST 4 cut(s) 58, 112, 537, 562
SfaNI GCATC 1 cut(s) 256
SmiMI CAYNNNNRTG 2 cut(s) 122, 494
SmlI CTYRAG 2 cut(s) 41, 500
SmoI CTYRAG 2 cut(s) 41, 500
Sse9I AATT 4 cut(s) 27, 178, 290, 333
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 1 cut(s) 211
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 1 cut(s) 137
TaaI ACNGT 1 cut(s) 154
TasI AATT 4 cut(s) 27, 178, 290, 333
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 2 cut(s) 299, 505
Tru1I TTAA 3 cut(s) 30, 185, 494
Tru9I TTAA 3 cut(s) 30, 185, 494
TseFI GTSAC 1 cut(s) 485
TseI GCWGC 2 cut(s) 217, 401
Tsp45I GTSAC 1 cut(s) 485
TspDTI ATGAA 6 cut(s) 48, 140, 212, 291, 303, 367
TspGWI ACGGA 1 cut(s) 353
VspI ATTAAT 1 cut(s) 30
XceI RCATGY 1 cut(s) 493
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.