RchiOBHm_Chr4g0427551

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002269
Physical Location & Seq
Forward (+)
52777453 .. 52778923
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39647

Sequence Viewer

Length: 1332 bp
ATGGCTGGCACCGGATGGATTATTCAAGTTAATGAAGATCTCAAGGACATGGAAGGTCTTTCAACAGAGAAGCATCACTGGAATAAGGGGTCAATCTACAAATTACCAGCCAGCCTCACAGACATTAACAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGGCCTTACCATTATGATCCTTCAAATCCAATGGAGGAACACAAGCATCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGTCTGTTGAGCTGTTTGTAGATGCATTGGCTGAAGTAGAGAATGATTTGAAGGACTCATATACTCTGCTTCATTCTGTGCCGAAAGAAGTTACAGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTAGAAATTTTAAGGACTGCTGCTCATGTACAGCTGGAAGATTATGCTCCTAATGATCCCATCTTCAGCAATCATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTAGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAACATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTCATACTCAAGTTCCTCTGCTCCTGCAATGTTTCTCCAAACATGGCAAAGGGTGTGCATGTATTGGATGTGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCACAAATATATTCGCCACAAAATAAGATCAATGGAGCGTGATGATGAGATTATCTGGTCCGCAACAGAGCTCAACGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCACCCGGGGAGTGCTGAGGCTCCCTGTTGTTCTTGTGGATGACACCACTGAGTCCATGTTTTTAAATCTGATGGCATTTGAGAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCAAGGGATGTCGCCCTCTTACATTCGAAAGGGATCATCCATAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTGTTCAATTCACTCTCTAAGGATGTGACACAAGACCCAGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATAGTTGGCGCGCCAATCTTATTCACACTTACTTCAGAAATCCATGGGCTCTCAGACTGGATATTCCATATATCCCTATTACCGTCCACCAAATGAATCTTCTCCTTCCACTGTCATTCTCGTTCCGGCTCCTCCTCCTCCAAATCATTCATCATCCGACTCCACCTCCCCATCAACACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

443

Amino Acids

51.0

Weight (kDa)

6.96

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 399 9.1e-111 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 3 cut(s) 213, 215, 1158
AciI CCGC 1 cut(s) 753
AclWI GGATC 3 cut(s) 173, 431, 1016
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 4 cut(s) 297, 430, 662, 1165
AfaI GTAC 2 cut(s) 411, 662
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 9 cut(s) 26, 63, 186, 295, 533, 551, 787, 1057, 1103
AjuI GAANNNNNNNTTGG 2 cut(s) 1230, 1262
AluBI AGCT 6 cut(s) 256, 415, 538, 563, 589, 763
AluI AGCT 6 cut(s) 256, 415, 538, 563, 589, 763
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 3 cut(s) 173, 431, 1016
Ama87I CYCGRG 1 cut(s) 828
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 3 cut(s) 217, 401, 1095
ApoI RAATTY 1 cut(s) 387
AscI GGCGCGCC 1 cut(s) 1156
AspLEI GCGC 4 cut(s) 215, 217, 1158, 1160
AspS9I GGNCC 2 cut(s) 164, 750
AsuC2I CCSGG 2 cut(s) 829, 830
AsuHPI GGTGA 2 cut(s) 817, 946
AsuII TTCGAA 1 cut(s) 1001
AvaI CYCGRG 1 cut(s) 828
AvaII GGWCC 1 cut(s) 750
BanI GGYRCC 1 cut(s) 8
BanII GRGCYC 2 cut(s) 765, 1198
Bbv12I GWGCWC 1 cut(s) 765
BbvCI CCTCAGC 1 cut(s) 839
BbvI GCAGC 3 cut(s) 204, 388, 1107
BccI CCATC 5 cut(s) 9, 362, 449, 889, 1326
BceAI ACGGC 1 cut(s) 1126
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 2 cut(s) 829, 830
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 2 cut(s) 383, 503
BfmI CTRYAG 1 cut(s) 1132
BglII AGATCT 1 cut(s) 37
BisI GCNGC 3 cut(s) 218, 402, 1096
BlsI GCNGC 3 cut(s) 219, 403, 1097
Bme1390I CCNGG 2 cut(s) 829, 830
Bme18I GGWCC 1 cut(s) 750
BmeT110I CYCGRG 1 cut(s) 828
BmgT120I GGNCC 2 cut(s) 164, 750
BmiI GGNNCC 4 cut(s) 10, 464, 845, 1277
BmrFI CCNGG 2 cut(s) 829, 830
BmsI GCATC 3 cut(s) 82, 217, 256
Bpu10I CCTNAGC 2 cut(s) 564, 839
Bpu14I TTCGAA 1 cut(s) 1001
BpuEI CTTGAG 2 cut(s) 26, 581
BpuMI CCSGG 2 cut(s) 829, 830
BsaJI CCNNGG 4 cut(s) 828, 829, 1142, 1190
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 1297, 1327
Bse1I ACTGG 2 cut(s) 83, 1209
Bse3DI GCAATG 1 cut(s) 622
BseDI CCNNGG 4 cut(s) 828, 829, 1142, 1190
BseGI GGATG 8 cut(s) 20, 574, 661, 868, 988, 1011, 1078, 1300
BseMI GCAATG 1 cut(s) 622
BseMII CTCAG 3 cut(s) 830, 864, 1213
BseNI ACTGG 2 cut(s) 83, 1209
BsePI GCGCGC 2 cut(s) 213, 1156
BseRI GAGGAG 3 cut(s) 1268, 1271, 1274
BseXI GCAGC 3 cut(s) 204, 388, 1107
Bsh1236I CGCG 3 cut(s) 213, 215, 1158
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 765
BsiHKCI CYCGRG 1 cut(s) 828
BsiSI CCGG 3 cut(s) 12, 829, 1273
BsmAI GTCTC 1 cut(s) 160
BsnI GGCC 2 cut(s) 137, 166
BsoBI CYCGRG 1 cut(s) 828
Bsp119I TTCGAA 1 cut(s) 1001
Bsp1286I GDGCHC 2 cut(s) 765, 1198
Bsp1407I TGTACA 2 cut(s) 409, 660
Bsp143I GATC 6 cut(s) 37, 178, 436, 691, 719, 1008
Bsp19I CCATGG 1 cut(s) 1190
BspACI CCGC 1 cut(s) 753
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 3 cut(s) 831, 865, 1212
BspFNI CGCG 3 cut(s) 213, 215, 1158
BspLI GGNNCC 4 cut(s) 10, 464, 845, 1277
BspMAI CTGCAG 1 cut(s) 1136
BspPI GGATC 3 cut(s) 173, 431, 1016
BspT104I TTCGAA 1 cut(s) 1001
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 622
BsrGI TGTACA 2 cut(s) 409, 660
BsrI ACTGG 2 cut(s) 83, 1209
BssECI CCNNGG 4 cut(s) 828, 829, 1142, 1190
BssHII GCGCGC 2 cut(s) 213, 1156
BssMI GATC 6 cut(s) 37, 178, 436, 691, 719, 1008
BssT1I CCWWGG 1 cut(s) 1190
Bst4CI ACNGT 3 cut(s) 154, 1231, 1260
Bst6I CTCTTC 2 cut(s) 662, 956
BstAUI TGTACA 2 cut(s) 409, 660
BstBI TTCGAA 1 cut(s) 1001
BstC8I GCNNGC 4 cut(s) 7, 112, 215, 1158
BstDEI CTNAG 5 cut(s) 564, 839, 873, 1068, 1199
BstDSI CCRYGG 2 cut(s) 1142, 1190
BstF5I GGATG 8 cut(s) 20, 574, 661, 868, 988, 1011, 1078, 1300
BstFNI CGCG 3 cut(s) 213, 215, 1158
BstHHI GCGC 4 cut(s) 215, 217, 1158, 1160
BstKTI GATC 6 cut(s) 40, 181, 439, 694, 722, 1011
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 6 cut(s) 37, 178, 436, 691, 719, 1008
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNSI RCATGY 2 cut(s) 496, 650
BstSCI CCNGG 2 cut(s) 827, 828
BstSFI CTRYAG 1 cut(s) 1132
BstUI CGCG 3 cut(s) 213, 215, 1158
BstV1I GCAGC 3 cut(s) 204, 388, 1107
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 2 cut(s) 1142, 1190
BtgZI GCGATG 1 cut(s) 194
BtsCI GGATG 8 cut(s) 20, 574, 661, 868, 988, 1011, 1078, 1300
BtsI GCAGTG 1 cut(s) 1047
BtsIMutI CAGTG 4 cut(s) 76, 870, 1047, 1256
Cac8I GCNNGC 4 cut(s) 7, 112, 215, 1158
CfoI GCGC 4 cut(s) 215, 217, 1158, 1160
Cfr13I GGNCC 2 cut(s) 164, 750
Cfr9I CCCGGG 1 cut(s) 828
Csp6I GTAC 2 cut(s) 410, 661
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 2 cut(s) 410, 661
DdeI CTNAG 5 cut(s) 564, 839, 873, 1068, 1199
DpnI GATC 6 cut(s) 39, 180, 438, 693, 721, 1010
DpnII GATC 6 cut(s) 37, 178, 436, 691, 719, 1008
DraI TTTAAA 1 cut(s) 888
Eam1104I CTCTTC 2 cut(s) 662, 956
EarI CTCTTC 2 cut(s) 662, 956
Ecl136II GAGCTC 1 cut(s) 763
Eco130I CCWWGG 1 cut(s) 1190
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 2 cut(s) 765, 1198
Eco47I GGWCC 1 cut(s) 750
Eco53kI GAGCTC 1 cut(s) 763
Eco57I CTGAAG 4 cut(s) 297, 430, 662, 1165
Eco88I CYCGRG 1 cut(s) 828
EcoICRI GAGCTC 1 cut(s) 763
EcoT14I CCWWGG 1 cut(s) 1190
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 2 cut(s) 765, 1198
ErhI CCWWGG 1 cut(s) 1190
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 3 cut(s) 218, 402, 1096
FokI GGATG 8 cut(s) 27, 581, 668, 875, 995, 998, 1085, 1287
FriOI GRGCYC 2 cut(s) 765, 1198
Fsp4HI GCNGC 3 cut(s) 218, 402, 1096
FspBI CTAG 2 cut(s) 383, 503
GlaI GCGC 4 cut(s) 214, 216, 1157, 1159
GluI GCNGC 3 cut(s) 218, 402, 1096
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 3 cut(s) 12, 829, 1273
HhaI GCGC 4 cut(s) 215, 217, 1158, 1160
Hin6I GCGC 4 cut(s) 213, 215, 1156, 1158
HinP1I GCGC 4 cut(s) 213, 215, 1156, 1158
HinfI GANTC 8 cut(s) 299, 508, 670, 777, 875, 909, 1243, 1306
HpaII CCGG 3 cut(s) 12, 829, 1273
HphI GGTGA 2 cut(s) 817, 946
Hpy166II GTNNAC 2 cut(s) 661, 1234
Hpy188I TCNGA 4 cut(s) 894, 1184, 1202, 1305
Hpy188III TCNNGA 2 cut(s) 530, 787
Hpy8I GTNNAC 2 cut(s) 661, 1234
HpyAV CCTTC 8 cut(s) 47, 127, 169, 192, 289, 566, 1109, 1262
HpyCH4III ACNGT 3 cut(s) 154, 1231, 1260
HpyCH4V TGCA 8 cut(s) 269, 352, 615, 646, 797, 977, 1111, 1134
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 5 cut(s) 564, 839, 873, 1068, 1199
HspAI GCGC 4 cut(s) 213, 215, 1156, 1158
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 6 cut(s) 37, 178, 436, 691, 719, 1008
LmnI GCTCC 7 cut(s) 433, 468, 614, 727, 849, 920, 1281
Lsp1109I GCAGC 3 cut(s) 204, 388, 1107
LweI GCATC 3 cut(s) 82, 217, 256
MaeI CTAG 2 cut(s) 383, 503
MaeIII GTNAC 6 cut(s) 334, 488, 580, 934, 1031, 1075
MalI GATC 6 cut(s) 39, 180, 438, 693, 721, 1010
MboI GATC 6 cut(s) 37, 178, 436, 691, 719, 1008
MboII GAAGA 6 cut(s) 47, 431, 436, 679, 943, 1238
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 765, 1198
MluCI AATT 4 cut(s) 101, 387, 1057, 1104
MlyI GAGTC 4 cut(s) 293, 679, 884, 1300
MmeI TCCRAC 1 cut(s) 1328
Mph1103I ATGCAT 1 cut(s) 271
MseI TTAA 5 cut(s) 30, 126, 392, 497, 887
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 3 cut(s) 12, 829, 1273
MspR9I CCNGG 2 cut(s) 829, 830
MvnI CGCG 3 cut(s) 213, 215, 1158
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 2 cut(s) 829, 830
NcoI CCATGG 1 cut(s) 1190
NdeII GATC 6 cut(s) 37, 178, 436, 691, 719, 1008
NlaIV GGNNCC 4 cut(s) 10, 464, 845, 1277
NmuCI GTSAC 5 cut(s) 488, 580, 934, 1031, 1075
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 2 cut(s) 496, 650
NspV TTCGAA 1 cut(s) 1001
PalAI GGCGCGCC 1 cut(s) 1156
PauI GCGCGC 2 cut(s) 213, 1156
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 492
PfeI GAWTC 4 cut(s) 508, 777, 909, 1243
PkrI GCNGC 3 cut(s) 219, 403, 1097
PleI GAGTC 4 cut(s) 293, 678, 883, 1300
PpsI GAGTC 4 cut(s) 293, 678, 883, 1300
PscI ACATGT 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 765
PspN4I GGNNCC 4 cut(s) 10, 464, 845, 1277
PspPI GGNCC 2 cut(s) 164, 750
PstI CTGCAG 1 cut(s) 1136
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 2 cut(s) 213, 1156
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 2 cut(s) 411, 662
RsaNI GTAC 2 cut(s) 410, 661
RseI CAYNNNNRTG 1 cut(s) 497
SacI GAGCTC 1 cut(s) 765
SaqAI TTAA 5 cut(s) 30, 126, 392, 497, 887
SatI GCNGC 3 cut(s) 218, 402, 1096
Sau3AI GATC 6 cut(s) 37, 178, 436, 691, 719, 1008
Sau96I GGNCC 2 cut(s) 164, 750
SchI GAGTC 4 cut(s) 293, 679, 884, 1300
ScrFI CCNGG 2 cut(s) 829, 830
SduI GDGCHC 2 cut(s) 765, 1198
SfaNI GCATC 3 cut(s) 82, 217, 256
SfcI CTRYAG 1 cut(s) 1132
SfuI TTCGAA 1 cut(s) 1001
SgsI GGCGCGCC 1 cut(s) 1156
SinI GGWCC 1 cut(s) 750
SmaI CCCGGG 1 cut(s) 830
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 41, 596
SmoI CTYRAG 2 cut(s) 41, 596
Sse9I AATT 4 cut(s) 101, 387, 1057, 1104
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 1 cut(s) 753
SspMI CTAG 2 cut(s) 383, 503
SstI GAGCTC 1 cut(s) 765
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 827, 828
StyI CCWWGG 1 cut(s) 1190
TaaI ACNGT 3 cut(s) 154, 1231, 1260
TaqI TCGA 1 cut(s) 1001
TasI AATT 4 cut(s) 101, 387, 1057, 1104
TatI WGTACW 2 cut(s) 409, 660
TfiI GAWTC 4 cut(s) 508, 777, 909, 1243
Tru1I TTAA 5 cut(s) 30, 126, 392, 497, 887
Tru9I TTAA 5 cut(s) 30, 126, 392, 497, 887
TscAI CASTG 4 cut(s) 83, 877, 1047, 1263
TseFI GTSAC 5 cut(s) 488, 580, 934, 1031, 1075
TseI GCWGC 3 cut(s) 217, 401, 1095
Tsp45I GTSAC 5 cut(s) 488, 580, 934, 1031, 1075
TspDTI ATGAA 8 cut(s) 48, 212, 305, 367, 585, 943, 1256, 1286
TspMI CCCGGG 1 cut(s) 828
TspRI CASTG 4 cut(s) 83, 877, 1047, 1263
VpaK11BI GGWCC 1 cut(s) 750
XapI RAATTY 1 cut(s) 387
XceI RCATGY 2 cut(s) 496, 650
XmaI CCCGGG 1 cut(s) 828
XspI CTAG 2 cut(s) 383, 503
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.