Rh4CG296200

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002269
Physical Location & Seq
Forward (+)
55536543 .. 55538064
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG296200.1

Sequence Viewer

Length: 1425 bp
ATGGCTGGCACCGGATGGATTATTCAAGTTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAGAAGCATCACTGGAATAAGGGGTCAATCTACAAATTACCAGCCAGCCTCACAGACATGAACAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGGCCTTACCATTATGATCCTTCAAATCCAATGGAGGAGCACAAGCATCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGTCTGTTGAGCTGTTTGTAGATGCATTGGCTGAAGTAGAGAATGATTTGAAGGACTCATATACTCTGCTTCATTCTGTGCCGAAAGAAGTTACAGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTAGAAATTTTAAGGACTGCTGCTCATGTACAGCTGGAAGATTATGCTCCTAATGATCCCATCTTCGGCAATCATGCTAGGCTCCATGCCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGGGTATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTCATACTCAAGTTCCTCTGCTCCTGCAATGTTTCTCCAAACATGGGAAAGTGTGTGCATGTATTGGACGTGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCACAAATATATTCGCCACAATATAAGGTTAATGGAGCGTGACGATGAGATCATCTGGTCTGCAACGGCGCTCAATGAAGCAGGAATCAGGTTCAAGAAAAGTACAACTACAAGCCTCAAAGACATCACATTCACCCGGGGAGTGCTGAGGCTCCCTGTTGTTCTTGTGGATGACACCACTGAGTCCATGTTTTTAAATCTGATGGCATTTGAGAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCAAGGGATGTCGCCCTCTTACATTCGAAAGGGATCATCCATAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTGTTCAATTCACTCTCTAAGGATGTGACACAAGACCCAGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATAGGTGGCGCGCCAATCTTATTCACACTTACTTCAGAAATCCATGGGCTATTATCTCTTTCGTTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATTCCATATATCCCTATTACTTTCCACGAAATGATTCTTCTCCTTCCACTGTCATTCTTGTTCCGGCAGCTCCTTCTCCTCCAAACCATTCATCATCCGACTCCACCTGCCCATCAACACTAGTGATTTCTTTCATTCTTGTTTCCATGGTATGCATGCTTACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

474

Amino Acids

53.91

Weight (kDa)

7.06

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 411 1.7e-114 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1370
Acc36I ACCTGC 1 cut(s) 1370
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 3 cut(s) 213, 215, 1158
AclWI GGATC 3 cut(s) 173, 431, 1016
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 3 cut(s) 297, 662, 1165
AfaI GTAC 3 cut(s) 411, 662, 796
AfiI CCNNNNNNNGG 2 cut(s) 446, 632
AflIII ACRYGT 2 cut(s) 492, 657
AgsI TTSAA 8 cut(s) 26, 63, 186, 295, 533, 787, 1057, 1103
AhlI ACTAGT 1 cut(s) 1375
AjiI CACGTC 1 cut(s) 658
AluBI AGCT 7 cut(s) 256, 415, 538, 563, 589, 1244, 1325
AluI AGCT 7 cut(s) 256, 415, 538, 563, 589, 1244, 1325
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 3 cut(s) 173, 431, 1016
Ama87I CYCGRG 1 cut(s) 828
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 4 cut(s) 217, 401, 1095, 1322
ApoI RAATTY 1 cut(s) 387
AscI GGCGCGCC 1 cut(s) 1156
Asp700I GAANNNNTTC 1 cut(s) 1288
AspLEI GCGC 5 cut(s) 215, 217, 763, 1158, 1160
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 2 cut(s) 829, 830
AsuHPI GGTGA 2 cut(s) 817, 946
AsuII TTCGAA 1 cut(s) 1001
AvaI CYCGRG 1 cut(s) 828
BanI GGYRCC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 1213
Bbv12I GWGCWC 1 cut(s) 204
BbvCI CCTCAGC 1 cut(s) 839
BbvI GCAGC 4 cut(s) 204, 388, 1107, 1334
BccI CCATC 5 cut(s) 9, 362, 449, 889, 1375
BceAI ACGGC 3 cut(s) 455, 774, 1126
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 2 cut(s) 829, 830
BcoDI GTCTC 1 cut(s) 160
BcuI ACTAGT 1 cut(s) 1375
BfaI CTAG 3 cut(s) 383, 459, 1376
BfmI CTRYAG 2 cut(s) 1132, 1239
BfoI RGCGCY 1 cut(s) 764
BfuAI ACCTGC 1 cut(s) 1370
BglII AGATCT 1 cut(s) 37
BisI GCNGC 4 cut(s) 218, 402, 1096, 1323
BlsI GCNGC 4 cut(s) 219, 403, 1097, 1324
Bme1390I CCNGG 2 cut(s) 829, 830
BmeT110I CYCGRG 1 cut(s) 828
BmgBI CACGTC 1 cut(s) 658
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 3 cut(s) 10, 464, 845
BmrFI CCNGG 2 cut(s) 829, 830
BmsI GCATC 3 cut(s) 82, 217, 256
BpiI GAAGAC 1 cut(s) 1213
Bpu10I CCTNAGC 2 cut(s) 564, 839
Bpu14I TTCGAA 1 cut(s) 1001
BpuEI CTTGAG 3 cut(s) 26, 524, 581
BpuMI CCSGG 2 cut(s) 829, 830
BsaJI CCNNGG 5 cut(s) 828, 829, 1142, 1190, 1401
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 632
Bse1I ACTGG 2 cut(s) 83, 1255
Bse3DI GCAATG 1 cut(s) 622
BseDI CCNNGG 5 cut(s) 828, 829, 1142, 1190, 1401
BseGI GGATG 7 cut(s) 20, 574, 868, 988, 1011, 1078, 1349
BseLI CCNNNNNNNGG 2 cut(s) 446, 632
BseMI GCAATG 1 cut(s) 622
BseMII CTCAG 3 cut(s) 830, 864, 1259
BseNI ACTGG 2 cut(s) 83, 1255
BsePI GCGCGC 2 cut(s) 213, 1156
BseRI GAGGAG 2 cut(s) 212, 1323
BseXI GCAGC 4 cut(s) 204, 388, 1107, 1334
Bsh1236I CGCG 3 cut(s) 213, 215, 1158
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 828
BsiSI CCGG 3 cut(s) 12, 829, 1319
BslI CCNNNNNNNGG 2 cut(s) 446, 632
BsmAI GTCTC 1 cut(s) 160
BsnI GGCC 2 cut(s) 137, 166
BsoBI CYCGRG 1 cut(s) 828
Bsp119I TTCGAA 1 cut(s) 1001
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 2 cut(s) 409, 660
Bsp143I GATC 6 cut(s) 37, 178, 436, 691, 741, 1008
Bsp19I CCATGG 2 cut(s) 1190, 1401
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 3 cut(s) 831, 865, 1258
BspFNI CGCG 3 cut(s) 213, 215, 1158
BspLI GGNNCC 3 cut(s) 10, 464, 845
BspMAI CTGCAG 1 cut(s) 1136
BspMI ACCTGC 1 cut(s) 1370
BspPI GGATC 3 cut(s) 173, 431, 1016
BspT104I TTCGAA 1 cut(s) 1001
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 622
BsrGI TGTACA 2 cut(s) 409, 660
BsrI ACTGG 2 cut(s) 83, 1255
BssECI CCNNGG 5 cut(s) 828, 829, 1142, 1190, 1401
BssHII GCGCGC 2 cut(s) 213, 1156
BssMI GATC 6 cut(s) 37, 178, 436, 691, 741, 1008
BssT1I CCWWGG 2 cut(s) 1190, 1401
Bst4CI ACNGT 2 cut(s) 154, 1306
Bst6I CTCTTC 2 cut(s) 662, 956
BstAUI TGTACA 2 cut(s) 409, 660
BstBI TTCGAA 1 cut(s) 1001
BstC8I GCNNGC 5 cut(s) 7, 112, 215, 1158, 1412
BstDEI CTNAG 5 cut(s) 564, 839, 873, 1068, 1245
BstDSI CCRYGG 3 cut(s) 1142, 1190, 1401
BstF5I GGATG 7 cut(s) 20, 574, 868, 988, 1011, 1078, 1349
BstFNI CGCG 3 cut(s) 213, 215, 1158
BstH2I RGCGCY 1 cut(s) 764
BstHHI GCGC 5 cut(s) 215, 217, 763, 1158, 1160
BstKTI GATC 6 cut(s) 40, 181, 439, 694, 744, 1011
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 6 cut(s) 37, 178, 436, 691, 741, 1008
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNSI RCATGY 3 cut(s) 496, 650, 1414
BstSCI CCNGG 2 cut(s) 827, 828
BstSFI CTRYAG 2 cut(s) 1132, 1239
BstUI CGCG 3 cut(s) 213, 215, 1158
BstV1I GCAGC 4 cut(s) 204, 388, 1107, 1334
BstV2I GAAGAC 1 cut(s) 1213
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 3 cut(s) 1142, 1190, 1401
BtgZI GCGATG 1 cut(s) 194
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 7 cut(s) 20, 574, 868, 988, 1011, 1078, 1349
BtsI GCAGTG 1 cut(s) 1047
BtsIMutI CAGTG 4 cut(s) 76, 870, 1047, 1302
BveI ACCTGC 1 cut(s) 1370
Cac8I GCNNGC 5 cut(s) 7, 112, 215, 1158, 1412
CfoI GCGC 5 cut(s) 215, 217, 763, 1158, 1160
Cfr13I GGNCC 1 cut(s) 164
Cfr9I CCCGGG 1 cut(s) 828
Csp6I GTAC 3 cut(s) 410, 661, 795
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 3 cut(s) 410, 661, 795
DdeI CTNAG 5 cut(s) 564, 839, 873, 1068, 1245
DpnI GATC 6 cut(s) 39, 180, 438, 693, 743, 1010
DpnII GATC 6 cut(s) 37, 178, 436, 691, 741, 1008
DraI TTTAAA 1 cut(s) 888
Eam1104I CTCTTC 2 cut(s) 662, 956
EarI CTCTTC 2 cut(s) 662, 956
Eco130I CCWWGG 2 cut(s) 1190, 1401
Eco147I AGGCCT 1 cut(s) 137
Eco57I CTGAAG 3 cut(s) 297, 662, 1165
Eco88I CYCGRG 1 cut(s) 828
EcoT14I CCWWGG 2 cut(s) 1190, 1401
EcoT22I ATGCAT 2 cut(s) 271, 1412
ErhI CCWWGG 2 cut(s) 1190, 1401
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 218, 402, 1096, 1323
FokI GGATG 7 cut(s) 27, 581, 875, 995, 998, 1085, 1336
Fsp4HI GCNGC 4 cut(s) 218, 402, 1096, 1323
FspBI CTAG 3 cut(s) 383, 459, 1376
GlaI GCGC 5 cut(s) 214, 216, 762, 1157, 1159
GluI GCNGC 4 cut(s) 218, 402, 1096, 1323
HaeII RGCGCY 1 cut(s) 764
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 3 cut(s) 12, 829, 1319
HhaI GCGC 5 cut(s) 215, 217, 763, 1158, 1160
Hin6I GCGC 5 cut(s) 213, 215, 761, 1156, 1158
HinP1I GCGC 5 cut(s) 213, 215, 761, 1156, 1158
HinfI GANTC 8 cut(s) 299, 508, 670, 777, 875, 909, 1289, 1355
HpaII CCGG 3 cut(s) 12, 829, 1319
HphI GGTGA 2 cut(s) 817, 946
Hpy166II GTNNAC 2 cut(s) 661, 1236
Hpy188I TCNGA 4 cut(s) 894, 1184, 1248, 1354
Hpy188III TCNNGA 3 cut(s) 43, 530, 787
Hpy8I GTNNAC 2 cut(s) 661, 1236
HpyAV CCTTC 9 cut(s) 47, 127, 169, 192, 289, 566, 1109, 1308, 1338
HpyCH4III ACNGT 2 cut(s) 154, 1306
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 9 cut(s) 269, 352, 615, 646, 755, 977, 1111, 1134, 1410
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 5 cut(s) 564, 839, 873, 1068, 1245
HpySE526I ACGT 1 cut(s) 657
HspAI GCGC 5 cut(s) 213, 215, 761, 1156, 1158
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 6 cut(s) 37, 178, 436, 691, 741, 1008
LmnI GCTCC 8 cut(s) 199, 433, 468, 614, 727, 849, 920, 1330
Lsp1109I GCAGC 4 cut(s) 204, 388, 1107, 1334
LweI GCATC 3 cut(s) 82, 217, 256
MaeI CTAG 3 cut(s) 383, 459, 1376
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 7 cut(s) 334, 488, 580, 731, 934, 1031, 1075
MalI GATC 6 cut(s) 39, 180, 438, 693, 743, 1010
MboI GATC 6 cut(s) 37, 178, 436, 691, 741, 1008
MboII GAAGA 7 cut(s) 47, 431, 436, 679, 943, 1213, 1284
MfeI CAATTG 1 cut(s) 1420
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 5 cut(s) 101, 387, 1057, 1104, 1420
MlyI GAGTC 4 cut(s) 293, 679, 884, 1349
MmeI TCCRAC 1 cut(s) 1377
Mph1103I ATGCAT 2 cut(s) 271, 1412
MroXI GAANNNNTTC 1 cut(s) 1288
MseI TTAA 5 cut(s) 30, 392, 497, 722, 887
MslI CAYNNNNRTG 2 cut(s) 122, 497
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 3 cut(s) 12, 829, 1319
MspR9I CCNGG 2 cut(s) 829, 830
MunI CAATTG 1 cut(s) 1420
MvnI CGCG 3 cut(s) 213, 215, 1158
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 2 cut(s) 829, 830
NcoI CCATGG 2 cut(s) 1190, 1401
NdeII GATC 6 cut(s) 37, 178, 436, 691, 741, 1008
NlaIV GGNNCC 3 cut(s) 10, 464, 845
NmuCI GTSAC 6 cut(s) 488, 580, 731, 934, 1031, 1075
NsiI ATGCAT 2 cut(s) 271, 1412
NspI RCATGY 3 cut(s) 496, 650, 1414
NspV TTCGAA 1 cut(s) 1001
PaeI GCATGC 1 cut(s) 1414
PalAI GGCGCGCC 1 cut(s) 1156
PaqCI CACCTGC 1 cut(s) 1370
PauI GCGCGC 2 cut(s) 213, 1156
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 492
PdmI GAANNNNTTC 1 cut(s) 1288
PfeI GAWTC 4 cut(s) 508, 777, 909, 1289
PkrI GCNGC 4 cut(s) 219, 403, 1097, 1324
PleI GAGTC 4 cut(s) 293, 678, 883, 1349
PpsI GAGTC 4 cut(s) 293, 678, 883, 1349
PscI ACATGT 1 cut(s) 492
PspN4I GGNNCC 3 cut(s) 10, 464, 845
PspPI GGNCC 1 cut(s) 164
PstI CTGCAG 1 cut(s) 1136
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 2 cut(s) 213, 1156
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 3 cut(s) 411, 662, 796
RsaNI GTAC 3 cut(s) 410, 661, 795
RseI CAYNNNNRTG 2 cut(s) 122, 497
SaqAI TTAA 5 cut(s) 30, 392, 497, 722, 887
SatI GCNGC 4 cut(s) 218, 402, 1096, 1323
Sau3AI GATC 6 cut(s) 37, 178, 436, 691, 741, 1008
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 4 cut(s) 293, 679, 884, 1349
ScrFI CCNGG 2 cut(s) 829, 830
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 3 cut(s) 82, 217, 256
SfcI CTRYAG 2 cut(s) 1132, 1239
SfuI TTCGAA 1 cut(s) 1001
SgsI GGCGCGCC 1 cut(s) 1156
SmaI CCCGGG 1 cut(s) 830
SmiMI CAYNNNNRTG 2 cut(s) 122, 497
SmlI CTYRAG 3 cut(s) 41, 503, 596
SmoI CTYRAG 3 cut(s) 41, 503, 596
SpeI ACTAGT 1 cut(s) 1375
SphI GCATGC 1 cut(s) 1414
Sse9I AATT 5 cut(s) 101, 387, 1057, 1104, 1420
SseBI AGGCCT 1 cut(s) 137
SspMI CTAG 3 cut(s) 383, 459, 1376
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 827, 828
StyI CCWWGG 2 cut(s) 1190, 1401
TaaI ACNGT 2 cut(s) 154, 1306
TaiI ACGT 1 cut(s) 660
TaqI TCGA 1 cut(s) 1001
TasI AATT 5 cut(s) 101, 387, 1057, 1104, 1420
TatI WGTACW 3 cut(s) 409, 660, 794
TfiI GAWTC 4 cut(s) 508, 777, 909, 1289
Tru1I TTAA 5 cut(s) 30, 392, 497, 722, 887
Tru9I TTAA 5 cut(s) 30, 392, 497, 722, 887
TscAI CASTG 4 cut(s) 83, 877, 1047, 1309
TseFI GTSAC 6 cut(s) 488, 580, 731, 934, 1031, 1075
TseI GCWGC 4 cut(s) 217, 401, 1095, 1322
Tsp45I GTSAC 6 cut(s) 488, 580, 731, 934, 1031, 1075
TspMI CCCGGG 1 cut(s) 828
TspRI CASTG 4 cut(s) 83, 877, 1047, 1309
XapI RAATTY 1 cut(s) 387
XceI RCATGY 3 cut(s) 496, 650, 1414
XmaI CCCGGG 1 cut(s) 828
XmnI GAANNNNTTC 1 cut(s) 1288
XspI CTAG 3 cut(s) 383, 459, 1376
Zsp2I ATGCAT 2 cut(s) 271, 1412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.