RchiOBHm_Chr4g0428321

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002269
Physical Location & Seq
Reverse (-)
53368115 .. 53369616
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39723

Sequence Viewer

Length: 1410 bp
ATGGCTGGCACCGGATGGATTATTCAAATTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAACAGGATCACTGGAATAAGGGGTCAATCTACAAACTACCAGCTAGCCTCACAGACATGAACAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGGCCTTACCATTATGATCCTTCAAATCCAATGGAGGAGCACAAGCACCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGCCTGTTGAGTTGTTTGTAGATGCATTGGCTGAAGTAGAGAATGAATTGAAGGATTCATATACTCTGCTTCGTTCTGTGCCGAAAGAAATTACGGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTGGAAATCATGAATGCTGCTCGTGTACTGGGTGATTATGCTCCTAATGATCCCATCTTCGGAGAACATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAACATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTAATAGTCAAGTTCCTCTGCTCCAGTAACGTTTCTCCAAACATGGGCAAGCGTGTGCATGTGTTGGACGTGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCCCAAATATAATCACTACAAAATAAGGTCAAGTAAGCATGACGATGAGATCATCTGGTCCGCAACAGCGCTTTATGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCGCCCGGGGAGTGTTGAAGCTCCCTGTTGTTGTTGTGGATGACACCACCGAGTCCATGTTCTTGAATCTGATGGCGTTTGAAAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCTATGGATGTTGCCCTCTTACACTCGAAAGGGATCATCCAGAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTGTTCAATTCACTGTCTAAGGATGTGATACAGGACCCGGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATGAGTGGCGCGCCAATCTTATTCACACTTACTTCCGAAATCCATGGGCTATTATCTCTTTCATTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATGCCATATATCCCTATTACCATCCACCAAATGATTCTTCTCCTTCCACTGTCATTCTCGTTCCGGCTCCTCCTCCAAAACATTCATCCTCCGACTCCACCTCCCCATCGACACTTGTGATTTCTTTCATCGTTAGCATGGTATGCATGCTGACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

469

Amino Acids

53.2

Weight (kDa)

6.55

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 409 3.6e-114 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 3 cut(s) 213, 215, 1152
AciI CCGC 2 cut(s) 211, 747
AclI AACGTT 1 cut(s) 612
AclWI GGATC 5 cut(s) 81, 173, 425, 680, 1010
AcuI CTGAAG 2 cut(s) 297, 656
AfaI GTAC 2 cut(s) 408, 656
AfeI AGCGCT 1 cut(s) 756
AfiI CCNNNNNNNGG 2 cut(s) 440, 626
AflIII ACRYGT 2 cut(s) 486, 651
AjiI CACGTC 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 1270, 1302
AluBI AGCT 6 cut(s) 110, 532, 557, 583, 838, 1238
AluI AGCT 6 cut(s) 110, 532, 557, 583, 838, 1238
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 5 cut(s) 81, 173, 425, 680, 1010
Ama87I CYCGRG 1 cut(s) 822
Aor51HI AGCGCT 1 cut(s) 756
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 3 cut(s) 217, 398, 1089
ArsI GACNNNNNNTTYG 2 cut(s) 800, 832
AscI GGCGCGCC 1 cut(s) 1150
AseI ATTAAT 1 cut(s) 30
AspLEI GCGC 5 cut(s) 215, 217, 757, 1152, 1154
AspS9I GGNCC 3 cut(s) 164, 744, 1078
AsuC2I CCSGG 3 cut(s) 823, 824, 1082
AsuHPI GGTGA 2 cut(s) 425, 940
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 822
AvaII GGWCC 2 cut(s) 744, 1078
BanI GGYRCC 1 cut(s) 8
BauI CACGAG 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 1207
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 3 cut(s) 204, 385, 1101
BccI CCATC 6 cut(s) 9, 362, 443, 883, 1278, 1363
BceAI ACGGC 1 cut(s) 1120
BcnI CCSGG 3 cut(s) 823, 824, 1082
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 2 cut(s) 1126, 1233
BfoI RGCGCY 1 cut(s) 758
BglII AGATCT 1 cut(s) 37
BisI GCNGC 3 cut(s) 218, 399, 1090
BlsI GCNGC 3 cut(s) 219, 400, 1091
Bme1390I CCNGG 3 cut(s) 823, 824, 1082
Bme18I GGWCC 2 cut(s) 744, 1078
BmeT110I CYCGRG 1 cut(s) 822
BmgBI CACGTC 1 cut(s) 652
BmgT120I GGNCC 3 cut(s) 164, 744, 1078
BmiI GGNNCC 4 cut(s) 10, 458, 1080, 1317
BmrFI CCNGG 3 cut(s) 823, 824, 1082
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 256
BmtI GCTAGC 1 cut(s) 114
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 1207
BpmI CTGGAG 1 cut(s) 589
Bpu10I CCTNAGC 1 cut(s) 558
BpuEI CTTGAG 2 cut(s) 26, 518
BpuMI CCSGG 3 cut(s) 823, 824, 1082
BsaJI CCNNGG 4 cut(s) 822, 823, 1136, 1184
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 1334, 1364
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 626
Bse1I ACTGG 4 cut(s) 83, 414, 606, 1249
Bse3DI GCAATG 1 cut(s) 1203
BseDI CCNNGG 4 cut(s) 822, 823, 1136, 1184
BseGI GGATG 8 cut(s) 20, 568, 862, 982, 1005, 1072, 1270, 1334
BseLI CCNNNNNNNGG 2 cut(s) 440, 626
BseMI GCAATG 1 cut(s) 1203
BseMII CTCAG 1 cut(s) 1253
BseNI ACTGG 4 cut(s) 83, 414, 606, 1249
BsePI GCGCGC 2 cut(s) 213, 1150
BseRI GAGGAG 3 cut(s) 212, 1308, 1311
BseXI GCAGC 3 cut(s) 204, 385, 1101
Bsh1236I CGCG 3 cut(s) 213, 215, 1152
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 4 cut(s) 12, 823, 1082, 1313
BslI CCNNNNNNNGG 2 cut(s) 440, 626
BsmAI GTCTC 1 cut(s) 160
BsmI GAATGC 2 cut(s) 400, 1018
BsnI GGCC 2 cut(s) 137, 166
BsoBI CYCGRG 1 cut(s) 822
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 654
Bsp143I GATC 7 cut(s) 37, 73, 178, 430, 685, 735, 1002
Bsp19I CCATGG 1 cut(s) 1184
BspACI CCGC 2 cut(s) 211, 747
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 1 cut(s) 1252
BspFNI CGCG 3 cut(s) 213, 215, 1152
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 4 cut(s) 10, 458, 1080, 1317
BspMAI CTGCAG 1 cut(s) 1130
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 5 cut(s) 81, 173, 425, 680, 1010
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 1203
BsrGI TGTACA 1 cut(s) 654
BsrI ACTGG 4 cut(s) 83, 414, 606, 1249
BssECI CCNNGG 4 cut(s) 822, 823, 1136, 1184
BssHII GCGCGC 2 cut(s) 213, 1150
BssMI GATC 7 cut(s) 37, 73, 178, 430, 685, 735, 1002
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 1184
Bst2BI CACGAG 1 cut(s) 402
Bst4CI ACNGT 3 cut(s) 154, 1059, 1300
Bst6I CTCTTC 2 cut(s) 656, 950
BstAPI GCANNNNNTGC 1 cut(s) 1392
BstAUI TGTACA 1 cut(s) 654
BstC8I GCNNGC 6 cut(s) 7, 112, 215, 632, 1152, 1397
BstDEI CTNAG 3 cut(s) 558, 1062, 1239
BstDSI CCRYGG 2 cut(s) 1136, 1184
BstF5I GGATG 8 cut(s) 20, 568, 862, 982, 1005, 1072, 1270, 1334
BstFNI CGCG 3 cut(s) 213, 215, 1152
BstH2I RGCGCY 1 cut(s) 758
BstHHI GCGC 5 cut(s) 215, 217, 757, 1152, 1154
BstKTI GATC 7 cut(s) 40, 76, 181, 433, 688, 738, 1005
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 7 cut(s) 37, 73, 178, 430, 685, 735, 1002
BstMWI GCNNNNNNNGC 2 cut(s) 214, 1392
BstNSI RCATGY 3 cut(s) 490, 644, 1399
BstSCI CCNGG 3 cut(s) 821, 822, 1080
BstSFI CTRYAG 2 cut(s) 1126, 1233
BstUI CGCG 3 cut(s) 213, 215, 1152
BstV1I GCAGC 3 cut(s) 204, 385, 1101
BstV2I GAAGAC 1 cut(s) 1207
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 2 cut(s) 1136, 1184
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 8 cut(s) 20, 568, 862, 982, 1005, 1072, 1270, 1334
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 4 cut(s) 76, 1041, 1055, 1296
Cac8I GCNNGC 6 cut(s) 7, 112, 215, 632, 1152, 1397
CciI TCATGA 1 cut(s) 390
CfoI GCGC 5 cut(s) 215, 217, 757, 1152, 1154
Cfr13I GGNCC 3 cut(s) 164, 744, 1078
Cfr9I CCCGGG 1 cut(s) 822
Csp6I GTAC 2 cut(s) 407, 655
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 2 cut(s) 407, 655
DdeI CTNAG 3 cut(s) 558, 1062, 1239
DpnI GATC 7 cut(s) 39, 75, 180, 432, 687, 737, 1004
DpnII GATC 7 cut(s) 37, 73, 178, 430, 685, 735, 1002
Eam1104I CTCTTC 2 cut(s) 656, 950
EarI CTCTTC 2 cut(s) 656, 950
Eco130I CCWWGG 1 cut(s) 1184
Eco147I AGGCCT 1 cut(s) 137
Eco47I GGWCC 2 cut(s) 744, 1078
Eco47III AGCGCT 1 cut(s) 756
Eco57I CTGAAG 2 cut(s) 297, 656
Eco88I CYCGRG 1 cut(s) 822
EcoO109I RGGNCCY 1 cut(s) 1078
EcoT14I CCWWGG 1 cut(s) 1184
EcoT22I ATGCAT 2 cut(s) 271, 1397
ErhI CCWWGG 1 cut(s) 1184
Fnu4HI GCNGC 3 cut(s) 218, 399, 1090
FokI GGATG 8 cut(s) 27, 575, 869, 989, 992, 1079, 1257, 1321
Fsp4HI GCNGC 3 cut(s) 218, 399, 1090
FspBI CTAG 1 cut(s) 111
GlaI GCGC 5 cut(s) 214, 216, 756, 1151, 1153
GluI GCNGC 3 cut(s) 218, 399, 1090
GsuI CTGGAG 1 cut(s) 589
HaeII RGCGCY 1 cut(s) 758
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 4 cut(s) 12, 823, 1082, 1313
HhaI GCGC 5 cut(s) 215, 217, 757, 1152, 1154
Hin6I GCGC 5 cut(s) 213, 215, 755, 1150, 1152
HinP1I GCGC 5 cut(s) 213, 215, 755, 1150, 1152
HinfI GANTC 9 cut(s) 299, 502, 664, 771, 869, 883, 903, 1283, 1343
HpaII CCGG 4 cut(s) 12, 823, 1082, 1313
HphI GGTGA 2 cut(s) 425, 940
Hpy166II GTNNAC 3 cut(s) 407, 655, 1230
Hpy188I TCNGA 5 cut(s) 443, 888, 1178, 1242, 1342
Hpy188III TCNNGA 6 cut(s) 43, 391, 524, 781, 880, 1009
Hpy8I GTNNAC 3 cut(s) 407, 655, 1230
HpyAV CCTTC 8 cut(s) 47, 127, 169, 192, 289, 560, 1103, 1302
HpyCH4III ACNGT 3 cut(s) 154, 1059, 1300
HpyCH4IV ACGT 2 cut(s) 612, 651
HpyCH4V TGCA 7 cut(s) 269, 352, 640, 791, 1105, 1128, 1395
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 1392
HpyF3I CTNAG 3 cut(s) 558, 1062, 1239
HpySE526I ACGT 2 cut(s) 612, 651
HspAI GCGC 5 cut(s) 213, 215, 755, 1150, 1152
Kzo9I GATC 7 cut(s) 37, 73, 178, 430, 685, 735, 1002
LmnI GCTCC 7 cut(s) 199, 427, 462, 608, 843, 914, 1321
Lsp1109I GCAGC 3 cut(s) 204, 385, 1101
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 111
MaeII ACGT 2 cut(s) 612, 651
MaeIII GTNAC 5 cut(s) 482, 574, 608, 928, 1025
MalI GATC 7 cut(s) 39, 75, 180, 432, 687, 737, 1004
MboI GATC 7 cut(s) 37, 73, 178, 430, 685, 735, 1002
MboII GAAGA 6 cut(s) 47, 430, 673, 937, 1207, 1278
MfeI CAATTG 1 cut(s) 1405
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 6 cut(s) 27, 290, 333, 1051, 1098, 1405
MlyI GAGTC 3 cut(s) 673, 878, 1337
MmeI TCCRAC 2 cut(s) 627, 1365
Mph1103I ATGCAT 2 cut(s) 271, 1397
MseI TTAA 2 cut(s) 30, 491
MslI CAYNNNNRTG 3 cut(s) 122, 491, 729
MspI CCGG 4 cut(s) 12, 823, 1082, 1313
MspR9I CCNGG 3 cut(s) 823, 824, 1082
MunI CAATTG 1 cut(s) 1405
Mva1269I GAATGC 2 cut(s) 400, 1018
MvnI CGCG 3 cut(s) 213, 215, 1152
MwoI GCNNNNNNNGC 2 cut(s) 214, 1392
NciI CCSGG 3 cut(s) 823, 824, 1082
NcoI CCATGG 1 cut(s) 1184
NdeII GATC 7 cut(s) 37, 73, 178, 430, 685, 735, 1002
NheI GCTAGC 1 cut(s) 110
NlaIV GGNNCC 4 cut(s) 10, 458, 1080, 1317
NmuCI GTSAC 4 cut(s) 482, 574, 928, 1025
NsiI ATGCAT 2 cut(s) 271, 1397
NspI RCATGY 3 cut(s) 490, 644, 1399
PaeI GCATGC 1 cut(s) 1399
PagI TCATGA 1 cut(s) 390
PalAI GGCGCGCC 1 cut(s) 1150
PauI GCGCGC 2 cut(s) 213, 1150
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 486
PctI GAATGC 2 cut(s) 400, 1018
PfeI GAWTC 6 cut(s) 299, 502, 771, 883, 903, 1283
PkrI GCNGC 3 cut(s) 219, 400, 1091
PleI GAGTC 3 cut(s) 672, 877, 1337
PpsI GAGTC 3 cut(s) 672, 877, 1337
PpuMI RGGWCCY 1 cut(s) 1078
PscI ACATGT 1 cut(s) 486
PshBI ATTAAT 1 cut(s) 30
Psp1406I AACGTT 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 1078
PspN4I GGNNCC 4 cut(s) 10, 458, 1080, 1317
PspPI GGNCC 3 cut(s) 164, 744, 1078
PspPPI RGGWCCY 1 cut(s) 1078
PstI CTGCAG 1 cut(s) 1130
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 2 cut(s) 213, 1150
RsaI GTAC 2 cut(s) 408, 656
RsaNI GTAC 2 cut(s) 407, 655
RseI CAYNNNNRTG 3 cut(s) 122, 491, 729
SaqAI TTAA 2 cut(s) 30, 491
SatI GCNGC 3 cut(s) 218, 399, 1090
Sau3AI GATC 7 cut(s) 37, 73, 178, 430, 685, 735, 1002
Sau96I GGNCC 3 cut(s) 164, 744, 1078
SchI GAGTC 3 cut(s) 673, 878, 1337
ScrFI CCNGG 3 cut(s) 823, 824, 1082
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 2 cut(s) 1126, 1233
SgsI GGCGCGCC 1 cut(s) 1150
SinI GGWCC 2 cut(s) 744, 1078
SmaI CCCGGG 1 cut(s) 824
SmiMI CAYNNNNRTG 3 cut(s) 122, 491, 729
SmlI CTYRAG 2 cut(s) 41, 497
SmoI CTYRAG 2 cut(s) 41, 497
SphI GCATGC 1 cut(s) 1399
Sse9I AATT 6 cut(s) 27, 290, 333, 1051, 1098, 1405
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 2 cut(s) 211, 747
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 3 cut(s) 821, 822, 1080
StyI CCWWGG 1 cut(s) 1184
TaaI ACNGT 3 cut(s) 154, 1059, 1300
TaiI ACGT 2 cut(s) 615, 654
TaqI TCGA 2 cut(s) 995, 1358
TasI AATT 6 cut(s) 27, 290, 333, 1051, 1098, 1405
TatI WGTACW 2 cut(s) 406, 654
TfiI GAWTC 6 cut(s) 299, 502, 771, 883, 903, 1283
Tru1I TTAA 2 cut(s) 30, 491
Tru9I TTAA 2 cut(s) 30, 491
TscAI CASTG 4 cut(s) 83, 1041, 1062, 1303
TseFI GTSAC 4 cut(s) 482, 574, 928, 1025
TseI GCWGC 3 cut(s) 217, 398, 1089
Tsp45I GTSAC 4 cut(s) 482, 574, 928, 1025
TspGWI ACGGA 1 cut(s) 353
TspMI CCCGGG 1 cut(s) 822
TspRI CASTG 4 cut(s) 83, 1041, 1062, 1303
VpaK11BI GGWCC 2 cut(s) 744, 1078
VspI ATTAAT 1 cut(s) 30
XceI RCATGY 3 cut(s) 490, 644, 1399
XmaI CCCGGG 1 cut(s) 822
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 2 cut(s) 271, 1397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.