Rroxscaffold_5G00369970

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002269
Physical Location & Seq
Reverse (-)
51685776 .. 51695074
9299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369970.1

Sequence Viewer

Length: 792 bp
ATGGCTGACATGAACTGGATTATTGAAGTTAATGAAGATCTTGATAGGATGGAAGGTGTTTCTACAGAGGAGCAGTGGAAGGGGTCTATTTACAAACTACCGGCCGGCATCACAGAGATAAACAAGAAGGCCTACAAGCCCCAGACAGTCTCCTTCGGCCCTTACCATTTCAATCATTCAAATCCGATGGAGAAGCACAAGCGTCGTGTGCTGCTTCATTTCCTCAAAAGATGTGAAAAACCTGTTGAACTGTTTGCAGATGCGCTTGCTGAAGTAGTGGAAGATGTGAAGGACTCATACAATCCTCTCGAGCCAGTGTGGAGAGATAATACAGCTAGATTTTTGCAACTCATGTTTCTGGATGGCAGTTTCATGCTTGAAATCTTGAAGACTGCTTCTCATATACTTGATGATTATGCTCCTACTGATCCTATCTTCAGCAAGCACGGTAAGTTTCATGTCATGCCCTTCATCAAACGCGACATGTTAATGCTCGGGAATCAGTTGCCAATGCTTGTTCTTGTCAAGCTGGTCGCAGTTGAAACTGATAAAGCTAAGGATGAAGAATCTGTGAATGGACTCATAGTAAAGTTCTGCTGCTCCTGCACAGTACCCCTGAAGTTGGGCAAGTGTGTGCACGTATTGGACGTGTACAGGAAGAGCCTGCTTCAGGAAGATCCCCACCGAGCTTACAGAAGCCGGAATCAGATTCAAGAAAAGCAAATCTGCAAGCCTCAAAGACATCTCATTCTCTGGGGAAATCCTGAAGCTCCCACGTATGGTTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

263

Amino Acids

30.36

Weight (kDa)

7.13

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 30 - 235 5.8e-43 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 480
AclWI GGATC 2 cut(s) 422, 671
AcoI YGGCCR 1 cut(s) 102
AcuI CTGAAG 5 cut(s) 291, 421, 638, 653, 786
AfaI GTAC 2 cut(s) 612, 653
AfiI CCNNNNNNNGG 3 cut(s) 622, 670, 779
AflIII ACRYGT 2 cut(s) 483, 648
AgsI TTSAA 8 cut(s) 26, 172, 180, 248, 380, 388, 542, 713
AjiI CACGTC 1 cut(s) 649
AluBI AGCT 5 cut(s) 335, 529, 554, 689, 770
AluI AGCT 5 cut(s) 335, 529, 554, 689, 770
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 1 cut(s) 154
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 2 cut(s) 422, 671
Ama87I CYCGRG 2 cut(s) 308, 494
AoxI GGCC 3 cut(s) 102, 129, 157
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 2 cut(s) 211, 597
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 1 cut(s) 158
AvaI CYCGRG 2 cut(s) 308, 494
BaeGI GKGCMC 1 cut(s) 639
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
BbsI GAAGAC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 198, 584
BccI CCATC 3 cut(s) 43, 181, 356
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 63
BglII AGATCT 1 cut(s) 37
BisI GCNGC 2 cut(s) 212, 598
BlsI GCNGC 2 cut(s) 213, 599
BmeT110I CYCGRG 2 cut(s) 308, 494
BmgBI CACGTC 1 cut(s) 649
BmgT120I GGNCC 1 cut(s) 158
BmsI GCATC 2 cut(s) 117, 250
BpiI GAAGAC 1 cut(s) 395
Bpu10I CCTNAGC 1 cut(s) 555
BsaAI YACGTR 2 cut(s) 640, 777
Bsc4I CCNNNNNNNGG 3 cut(s) 622, 670, 779
Bse118I RCCGGY 2 cut(s) 100, 104
Bse1I ACTGG 2 cut(s) 20, 314
BseGI GGATG 3 cut(s) 54, 367, 565
BseLI CCNNNNNNNGG 3 cut(s) 622, 670, 779
BseNI ACTGG 2 cut(s) 20, 314
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 639
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 198, 584
BsgI GTGCAG 1 cut(s) 589
Bsh1236I CGCG 1 cut(s) 480
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 3 cut(s) 104, 131, 159
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 639
BsiHKCI CYCGRG 2 cut(s) 308, 494
BsiSI CCGG 3 cut(s) 101, 105, 700
BslI CCNNNNNNNGG 3 cut(s) 622, 670, 779
BsmAI GTCTC 1 cut(s) 154
BsnI GGCC 3 cut(s) 104, 131, 159
BsoBI CYCGRG 2 cut(s) 308, 494
Bsp1286I GDGCHC 1 cut(s) 639
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 3 cut(s) 37, 427, 676
BspANI GGCC 3 cut(s) 104, 131, 159
BspFNI CGCG 1 cut(s) 480
BspPI GGATC 2 cut(s) 422, 671
BspQI GCTCTTC 1 cut(s) 653
BsrFI RCCGGY 2 cut(s) 100, 104
BsrGI TGTACA 1 cut(s) 651
BsrI ACTGG 2 cut(s) 20, 314
BssAI RCCGGY 2 cut(s) 100, 104
BssMI GATC 3 cut(s) 37, 427, 676
Bst4CI ACNGT 4 cut(s) 148, 252, 449, 610
Bst6I CTCTTC 1 cut(s) 653
BstAUI TGTACA 1 cut(s) 651
BstBAI YACGTR 2 cut(s) 640, 777
BstC8I GCNNGC 5 cut(s) 106, 267, 443, 665, 731
BstDEI CTNAG 1 cut(s) 555
BstENI CCTNNNNNAGG 1 cut(s) 668
BstF5I GGATG 3 cut(s) 54, 367, 565
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 3 cut(s) 40, 430, 679
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 3 cut(s) 37, 427, 676
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 208, 603
BstNSI RCATGY 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 639
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 2 cut(s) 198, 584
BstV2I GAAGAC 1 cut(s) 395
BstX2I RGATCY 2 cut(s) 37, 676
BstYI RGATCY 2 cut(s) 37, 676
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 3 cut(s) 104, 131, 159
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 3 cut(s) 54, 367, 565
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 2 cut(s) 80, 321
Cac8I GCNNGC 5 cut(s) 106, 267, 443, 665, 731
CfoI GCGC 1 cut(s) 265
Cfr10I RCCGGY 2 cut(s) 100, 104
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 191
Csp6I GTAC 2 cut(s) 611, 652
CviAII CATG 6 cut(s) 10, 352, 373, 458, 463, 484
CviQI GTAC 2 cut(s) 611, 652
DdeI CTNAG 1 cut(s) 555
DpnI GATC 3 cut(s) 39, 429, 678
DpnII GATC 3 cut(s) 37, 427, 676
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EclXI CGGCCG 1 cut(s) 102
Eco147I AGGCCT 1 cut(s) 131
Eco52I CGGCCG 1 cut(s) 102
Eco57I CTGAAG 5 cut(s) 291, 421, 638, 653, 786
Eco88I CYCGRG 2 cut(s) 308, 494
EcoNI CCTNNNNNAGG 1 cut(s) 668
FaeI CATG 6 cut(s) 13, 355, 376, 461, 466, 487
FatI CATG 6 cut(s) 9, 351, 372, 457, 462, 483
Fnu4HI GCNGC 2 cut(s) 212, 598
FokI GGATG 3 cut(s) 61, 374, 572
Fsp4HI GCNGC 2 cut(s) 212, 598
FspBI CTAG 1 cut(s) 336
GlaI GCGC 1 cut(s) 264
GluI GCNGC 2 cut(s) 212, 598
HaeIII GGCC 3 cut(s) 104, 131, 159
HapII CCGG 3 cut(s) 101, 105, 700
HgaI GACGC 1 cut(s) 191
HhaI GCGC 1 cut(s) 265
Hin1II CATG 6 cut(s) 13, 355, 376, 461, 466, 487
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HinfI GANTC 6 cut(s) 293, 499, 566, 579, 703, 709
HpaII CCGG 3 cut(s) 101, 105, 700
Hpy166II GTNNAC 2 cut(s) 637, 652
Hpy188I TCNGA 2 cut(s) 186, 708
Hpy188III TCNNGA 8 cut(s) 41, 308, 359, 385, 496, 671, 713, 764
Hpy8I GTNNAC 2 cut(s) 637, 652
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 6 cut(s) 47, 73, 121, 163, 283, 478
HpyCH4III ACNGT 4 cut(s) 148, 252, 449, 610
HpyCH4IV ACGT 3 cut(s) 639, 648, 776
HpyCH4V TGCA 5 cut(s) 257, 346, 606, 637, 729
HpyF10VI GCNNNNNNNGC 2 cut(s) 208, 603
HpyF3I CTNAG 1 cut(s) 555
HpySE526I ACGT 3 cut(s) 639, 648, 776
Hsp92II CATG 6 cut(s) 13, 355, 376, 461, 466, 487
HspAI GCGC 1 cut(s) 263
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 3 cut(s) 37, 427, 676
LguI GCTCTTC 1 cut(s) 653
LmnI GCTCC 4 cut(s) 70, 424, 605, 775
Lsp1109I GCAGC 2 cut(s) 198, 584
LweI GCATC 2 cut(s) 117, 250
MaeI CTAG 1 cut(s) 336
MaeII ACGT 3 cut(s) 639, 648, 776
MalI GATC 3 cut(s) 39, 429, 678
MboI GATC 3 cut(s) 37, 427, 676
MboII GAAGA 7 cut(s) 47, 293, 400, 427, 575, 670, 686
MflI RGATCY 2 cut(s) 37, 676
MhlI GDGCHC 1 cut(s) 639
MlyI GAGTC 2 cut(s) 287, 573
MnlI CCTC 4 cut(s) 61, 233, 315, 744
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 2 cut(s) 30, 488
MslI CAYNNNNRTG 1 cut(s) 488
MspI CCGG 3 cut(s) 101, 105, 700
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 2 cut(s) 208, 603
NaeI GCCGGC 1 cut(s) 106
NdeII GATC 3 cut(s) 37, 427, 676
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 6 cut(s) 13, 355, 376, 461, 466, 487
NspI RCATGY 1 cut(s) 487
PaeR7I CTCGAG 1 cut(s) 308
PceI AGGCCT 1 cut(s) 131
PciI ACATGT 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 653
PcsI WCGNNNNNNNCGW 1 cut(s) 645
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 4 cut(s) 499, 566, 703, 709
PkrI GCNGC 2 cut(s) 213, 599
PleI GAGTC 2 cut(s) 287, 573
PpsI GAGTC 2 cut(s) 287, 573
Ppu21I YACGTR 2 cut(s) 640, 777
PscI ACATGT 1 cut(s) 483
PspPI GGNCC 1 cut(s) 158
PsuI RGATCY 2 cut(s) 37, 676
RsaI GTAC 2 cut(s) 612, 653
RsaNI GTAC 2 cut(s) 611, 652
RseI CAYNNNNRTG 1 cut(s) 488
SapI GCTCTTC 1 cut(s) 653
SaqAI TTAA 2 cut(s) 30, 488
SatI GCNGC 2 cut(s) 212, 598
Sau3AI GATC 3 cut(s) 37, 427, 676
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 2 cut(s) 287, 573
SduI GDGCHC 1 cut(s) 639
SfaNI GCATC 2 cut(s) 117, 250
SfcI CTRYAG 1 cut(s) 63
Sfr274I CTCGAG 1 cut(s) 308
SlaI CTCGAG 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SseBI AGGCCT 1 cut(s) 131
SspMI CTAG 1 cut(s) 336
StuI AGGCCT 1 cut(s) 131
TaaI ACNGT 4 cut(s) 148, 252, 449, 610
TaiI ACGT 3 cut(s) 642, 651, 779
TaqI TCGA 1 cut(s) 309
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 4 cut(s) 499, 566, 703, 709
Tru1I TTAA 2 cut(s) 30, 488
Tru9I TTAA 2 cut(s) 30, 488
TscAI CASTG 2 cut(s) 80, 321
TseI GCWGC 2 cut(s) 211, 597
TspDTI ATGAA 7 cut(s) 26, 48, 206, 361, 446, 460, 576
TspRI CASTG 2 cut(s) 80, 321
VneI GTGCAC 1 cut(s) 635
XagI CCTNNNNNAGG 1 cut(s) 668
XceI RCATGY 1 cut(s) 487
XhoI CTCGAG 1 cut(s) 308
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.