Rw4G024430

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002269
Physical Location & Seq
Reverse (-)
50272778 .. 50274279
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024430.1

Sequence Viewer

Length: 1416 bp
ATGGCTGGCACCGGATGGATTATTCAAATTACTGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAACAGGATCACTGGAATAAGGGGTCAATCTACAAACTACCAGCTAGCCTCACAGACATGAACAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGGCCTTACCACTACAATTCCTTAAATCCAATGGAGGAGCACAAGCACCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGCCTGTTGAGTTGTTTGTAGATGCATTGGCTGAAGTAGAGAATGAATTGAAGGATTCATATACTCTGCTTCGTTCTGTGCCAAAAGAAATTACGGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTGGAAATCATAAGGAGTGCTGCTCATGTATTGGATGATTATGCTCCTAATGATCCCATCTTCGGAGAACATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAACATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTAATAGTCAAGTTCCTCTGCTCCAGTAACGTTTCTCCAAACATGGGCAAGTGTGTGCATGTGTTGGACATGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCCCAAATTTATTCGCTACAAAATAAGGTCAAGTGAGCATGACGATGAGATCATCTGGTCCGCAACAGCGCTCAATGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCGCCCGGGGAGTGTTGAGGCTCCCTGTTGTTGTTGTGGATGACACCACCGAGTCCATGTTCTTGAATCTGATGGCGTTTGAAAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCTATGGATGTTGCCCTCTTACACTCGAAAGGGATTATCCAGAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTCTTCAATTCACTGTCTAAGGATGTGACACAGGACCCAGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATGAGTGGCGCGCCAATCTTATTCACACTTACTTCCGAAATCCATGGGCTATTATCTCTTTCATTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGTTATGCCATATATCCCTATTACCATCCACCAAATGATTCTTCTCCTTCCACTGTCATTCTCGTTCCGGCTCCTCCTCCAAAACATTCATCCTCCAACTCCACCTCCCCATCGACACTTGTGATTTCTTTCATCCTCGTTACCATGGTATGCATGCTGACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

471

Amino Acids

53.42

Weight (kDa)

6.44

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 410 1.7e-115 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 3 cut(s) 213, 215, 1155
AciI CCGC 2 cut(s) 211, 750
AclI AACGTT 1 cut(s) 615
AclWI GGATC 3 cut(s) 81, 428, 683
AcsI RAATTY 1 cut(s) 695
AcuI CTGAAG 3 cut(s) 54, 297, 659
AfaI GTAC 1 cut(s) 659
AfeI AGCGCT 1 cut(s) 759
AfiI CCNNNNNNNGG 2 cut(s) 443, 629
AflIII ACRYGT 2 cut(s) 489, 654
AjuI GAANNNNNNNTTGG 2 cut(s) 1273, 1305
AluBI AGCT 5 cut(s) 110, 535, 560, 586, 1241
AluI AGCT 5 cut(s) 110, 535, 560, 586, 1241
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 3 cut(s) 81, 428, 683
Ama87I CYCGRG 1 cut(s) 825
Aor51HI AGCGCT 1 cut(s) 759
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 3 cut(s) 217, 401, 1092
ApoI RAATTY 1 cut(s) 695
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AscI GGCGCGCC 1 cut(s) 1153
AspLEI GCGC 5 cut(s) 215, 217, 760, 1155, 1157
AspS9I GGNCC 3 cut(s) 164, 747, 1081
AsuC2I CCSGG 2 cut(s) 826, 827
AsuHPI GGTGA 1 cut(s) 943
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 825
AvaII GGWCC 2 cut(s) 747, 1081
BanI GGYRCC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 1210
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 3 cut(s) 204, 388, 1104
BccI CCATC 6 cut(s) 9, 362, 446, 886, 1281, 1366
BceAI ACGGC 1 cut(s) 1123
BcnI CCSGG 2 cut(s) 826, 827
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 2 cut(s) 1129, 1236
BfoI RGCGCY 1 cut(s) 761
BglII AGATCT 1 cut(s) 37
BisI GCNGC 3 cut(s) 218, 402, 1093
BlsI GCNGC 3 cut(s) 219, 403, 1094
Bme1390I CCNGG 2 cut(s) 826, 827
Bme18I GGWCC 2 cut(s) 747, 1081
BmeT110I CYCGRG 1 cut(s) 825
BmgT120I GGNCC 3 cut(s) 164, 747, 1081
BmiI GGNNCC 5 cut(s) 10, 461, 842, 1083, 1320
BmrFI CCNGG 2 cut(s) 826, 827
BmsI GCATC 1 cut(s) 256
BmtI GCTAGC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 1210
BplI GAGNNNNNCTC 2 cut(s) 388, 420
BpmI CTGGAG 1 cut(s) 592
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 26, 521
BpuMI CCSGG 2 cut(s) 826, 827
BsaJI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1392
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 1337, 1367
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 629
Bse1I ACTGG 3 cut(s) 83, 609, 1252
Bse3DI GCAATG 1 cut(s) 1206
BseDI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1392
BseGI GGATG 9 cut(s) 20, 421, 571, 865, 985, 1075, 1273, 1337, 1380
BseLI CCNNNNNNNGG 2 cut(s) 443, 629
BseMI GCAATG 1 cut(s) 1206
BseMII CTCAG 1 cut(s) 1256
BseNI ACTGG 3 cut(s) 83, 609, 1252
BsePI GCGCGC 2 cut(s) 213, 1153
BseRI GAGGAG 3 cut(s) 212, 1311, 1314
BseXI GCAGC 3 cut(s) 204, 388, 1104
Bsh1236I CGCG 3 cut(s) 213, 215, 1155
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 825
BsiSI CCGG 3 cut(s) 12, 826, 1316
BslI CCNNNNNNNGG 2 cut(s) 443, 629
BsmAI GTCTC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 1021
BsnI GGCC 2 cut(s) 137, 166
BsoBI CYCGRG 1 cut(s) 825
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 5 cut(s) 37, 73, 433, 688, 738
Bsp19I CCATGG 2 cut(s) 1187, 1392
BspACI CCGC 2 cut(s) 211, 750
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 1 cut(s) 1255
BspFNI CGCG 3 cut(s) 213, 215, 1155
BspLI GGNNCC 5 cut(s) 10, 461, 842, 1083, 1320
BspMAI CTGCAG 1 cut(s) 1133
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 3 cut(s) 81, 428, 683
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 1206
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 3 cut(s) 83, 609, 1252
BssECI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1392
BssHII GCGCGC 2 cut(s) 213, 1153
BssMI GATC 5 cut(s) 37, 73, 433, 688, 738
BssT1I CCWWGG 2 cut(s) 1187, 1392
Bst4CI ACNGT 3 cut(s) 154, 1062, 1303
Bst6I CTCTTC 3 cut(s) 659, 953, 1055
BstAUI TGTACA 1 cut(s) 657
BstC8I GCNNGC 5 cut(s) 7, 112, 215, 1155, 1403
BstDEI CTNAG 3 cut(s) 561, 1065, 1242
BstDSI CCRYGG 3 cut(s) 1139, 1187, 1392
BstF5I GGATG 9 cut(s) 20, 421, 571, 865, 985, 1075, 1273, 1337, 1380
BstFNI CGCG 3 cut(s) 213, 215, 1155
BstH2I RGCGCY 1 cut(s) 761
BstHHI GCGC 5 cut(s) 215, 217, 760, 1155, 1157
BstKTI GATC 5 cut(s) 40, 76, 436, 691, 741
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 5 cut(s) 37, 73, 433, 688, 738
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNSI RCATGY 4 cut(s) 493, 647, 658, 1405
BstSCI CCNGG 2 cut(s) 824, 825
BstSFI CTRYAG 2 cut(s) 1129, 1236
BstUI CGCG 3 cut(s) 213, 215, 1155
BstV1I GCAGC 3 cut(s) 204, 388, 1104
BstV2I GAAGAC 1 cut(s) 1210
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 3 cut(s) 1139, 1187, 1392
BtsCI GGATG 9 cut(s) 20, 421, 571, 865, 985, 1075, 1273, 1337, 1380
BtsI GCAGTG 1 cut(s) 1044
BtsIMutI CAGTG 4 cut(s) 76, 1044, 1058, 1299
Cac8I GCNNGC 5 cut(s) 7, 112, 215, 1155, 1403
CfoI GCGC 5 cut(s) 215, 217, 760, 1155, 1157
Cfr13I GGNCC 3 cut(s) 164, 747, 1081
Cfr9I CCCGGG 1 cut(s) 825
Csp6I GTAC 1 cut(s) 658
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 1 cut(s) 658
DdeI CTNAG 3 cut(s) 561, 1065, 1242
DpnI GATC 5 cut(s) 39, 75, 435, 690, 740
DpnII GATC 5 cut(s) 37, 73, 433, 688, 738
Eam1104I CTCTTC 3 cut(s) 659, 953, 1055
EarI CTCTTC 3 cut(s) 659, 953, 1055
Eco130I CCWWGG 2 cut(s) 1187, 1392
Eco147I AGGCCT 1 cut(s) 137
Eco47I GGWCC 2 cut(s) 747, 1081
Eco47III AGCGCT 1 cut(s) 759
Eco57I CTGAAG 3 cut(s) 54, 297, 659
Eco88I CYCGRG 1 cut(s) 825
EcoO109I RGGNCCY 1 cut(s) 1081
EcoT14I CCWWGG 2 cut(s) 1187, 1392
EcoT22I ATGCAT 2 cut(s) 271, 1403
ErhI CCWWGG 2 cut(s) 1187, 1392
Fnu4HI GCNGC 3 cut(s) 218, 402, 1093
FokI GGATG 9 cut(s) 27, 428, 578, 872, 992, 1082, 1260, 1324, 1367
Fsp4HI GCNGC 3 cut(s) 218, 402, 1093
FspBI CTAG 1 cut(s) 111
GlaI GCGC 5 cut(s) 214, 216, 759, 1154, 1156
GluI GCNGC 3 cut(s) 218, 402, 1093
GsuI CTGGAG 1 cut(s) 592
HaeII RGCGCY 1 cut(s) 761
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 3 cut(s) 12, 826, 1316
HhaI GCGC 5 cut(s) 215, 217, 760, 1155, 1157
Hin6I GCGC 5 cut(s) 213, 215, 758, 1153, 1155
HinP1I GCGC 5 cut(s) 213, 215, 758, 1153, 1155
HinfI GANTC 8 cut(s) 299, 505, 667, 774, 872, 886, 906, 1286
HpaII CCGG 3 cut(s) 12, 826, 1316
HphI GGTGA 1 cut(s) 943
Hpy166II GTNNAC 1 cut(s) 1233
Hpy188I TCNGA 4 cut(s) 446, 891, 1181, 1245
Hpy188III TCNNGA 5 cut(s) 43, 527, 784, 883, 1012
Hpy8I GTNNAC 1 cut(s) 1233
HpyAV CCTTC 7 cut(s) 47, 127, 169, 289, 563, 1106, 1305
HpyCH4III ACNGT 3 cut(s) 154, 1062, 1303
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 7 cut(s) 269, 352, 643, 794, 1108, 1131, 1401
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 3 cut(s) 561, 1065, 1242
HpySE526I ACGT 1 cut(s) 615
HspAI GCGC 5 cut(s) 213, 215, 758, 1153, 1155
Kzo9I GATC 5 cut(s) 37, 73, 433, 688, 738
LmnI GCTCC 7 cut(s) 199, 430, 465, 611, 846, 917, 1324
Lsp1109I GCAGC 3 cut(s) 204, 388, 1104
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 111
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 7 cut(s) 485, 577, 611, 931, 1028, 1072, 1387
MalI GATC 5 cut(s) 39, 75, 435, 690, 740
MboI GATC 5 cut(s) 37, 73, 433, 688, 738
MboII GAAGA 7 cut(s) 47, 433, 676, 940, 1042, 1210, 1281
MfeI CAATTG 1 cut(s) 1411
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 8 cut(s) 27, 178, 290, 333, 695, 1054, 1101, 1411
MlyI GAGTC 2 cut(s) 676, 881
MmeI TCCRAC 2 cut(s) 630, 1368
Mph1103I ATGCAT 2 cut(s) 271, 1403
MseI TTAA 2 cut(s) 185, 494
MslI CAYNNNNRTG 3 cut(s) 122, 494, 732
MspI CCGG 3 cut(s) 12, 826, 1316
MspR9I CCNGG 2 cut(s) 826, 827
MunI CAATTG 1 cut(s) 1411
Mva1269I GAATGC 1 cut(s) 1021
MvnI CGCG 3 cut(s) 213, 215, 1155
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 2 cut(s) 826, 827
NcoI CCATGG 2 cut(s) 1187, 1392
NdeII GATC 5 cut(s) 37, 73, 433, 688, 738
NheI GCTAGC 1 cut(s) 110
NlaIV GGNNCC 5 cut(s) 10, 461, 842, 1083, 1320
NmuCI GTSAC 5 cut(s) 485, 577, 931, 1028, 1072
NsiI ATGCAT 2 cut(s) 271, 1403
NspI RCATGY 4 cut(s) 493, 647, 658, 1405
PaeI GCATGC 1 cut(s) 1405
PalAI GGCGCGCC 1 cut(s) 1153
PauI GCGCGC 2 cut(s) 213, 1153
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 2 cut(s) 489, 654
PctI GAATGC 1 cut(s) 1021
PfeI GAWTC 6 cut(s) 299, 505, 774, 886, 906, 1286
PkrI GCNGC 3 cut(s) 219, 403, 1094
PleI GAGTC 2 cut(s) 675, 880
PpsI GAGTC 2 cut(s) 675, 880
PpuMI RGGWCCY 1 cut(s) 1081
PscI ACATGT 2 cut(s) 489, 654
Psp1406I AACGTT 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 1081
PspN4I GGNNCC 5 cut(s) 10, 461, 842, 1083, 1320
PspPI GGNCC 3 cut(s) 164, 747, 1081
PspPPI RGGWCCY 1 cut(s) 1081
PstI CTGCAG 1 cut(s) 1133
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 2 cut(s) 213, 1153
RsaI GTAC 1 cut(s) 659
RsaNI GTAC 1 cut(s) 658
RseI CAYNNNNRTG 3 cut(s) 122, 494, 732
SaqAI TTAA 2 cut(s) 185, 494
SatI GCNGC 3 cut(s) 218, 402, 1093
Sau3AI GATC 5 cut(s) 37, 73, 433, 688, 738
Sau96I GGNCC 3 cut(s) 164, 747, 1081
SchI GAGTC 2 cut(s) 676, 881
ScrFI CCNGG 2 cut(s) 826, 827
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 2 cut(s) 1129, 1236
SgsI GGCGCGCC 1 cut(s) 1153
SinI GGWCC 2 cut(s) 747, 1081
SmaI CCCGGG 1 cut(s) 827
SmiMI CAYNNNNRTG 3 cut(s) 122, 494, 732
SmlI CTYRAG 2 cut(s) 41, 500
SmoI CTYRAG 2 cut(s) 41, 500
SphI GCATGC 1 cut(s) 1405
Sse9I AATT 8 cut(s) 27, 178, 290, 333, 695, 1054, 1101, 1411
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 2 cut(s) 211, 750
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 824, 825
StyI CCWWGG 2 cut(s) 1187, 1392
TaaI ACNGT 3 cut(s) 154, 1062, 1303
TaiI ACGT 1 cut(s) 618
TaqI TCGA 2 cut(s) 998, 1361
TasI AATT 8 cut(s) 27, 178, 290, 333, 695, 1054, 1101, 1411
TatI WGTACW 1 cut(s) 657
TfiI GAWTC 6 cut(s) 299, 505, 774, 886, 906, 1286
Tru1I TTAA 2 cut(s) 185, 494
Tru9I TTAA 2 cut(s) 185, 494
TscAI CASTG 4 cut(s) 83, 1044, 1065, 1306
TseFI GTSAC 5 cut(s) 485, 577, 931, 1028, 1072
TseI GCWGC 3 cut(s) 217, 401, 1092
Tsp45I GTSAC 5 cut(s) 485, 577, 931, 1028, 1072
TspGWI ACGGA 1 cut(s) 353
TspMI CCCGGG 1 cut(s) 825
TspRI CASTG 4 cut(s) 83, 1044, 1065, 1306
VpaK11BI GGWCC 2 cut(s) 747, 1081
XapI RAATTY 1 cut(s) 695
XceI RCATGY 4 cut(s) 493, 647, 658, 1405
XmaI CCCGGG 1 cut(s) 825
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 2 cut(s) 271, 1403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.