Rh4CG302700

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002269
Physical Location & Seq
Reverse (-)
56311309 .. 56312002
694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG302700.1

Sequence Viewer

Length: 564 bp
ATGGCTGACATGAACTGGATTATTGAAGTTAATGAAGATCTTAATAGGATGGAAGGTGTTTCTACAGAGGAGCAGTGGAAGGGGTCTATTTACAAACTTCCAGCCGGCATTGTAGACATAAACAAGAAGGCCTACAAGCCCCAGACAGTCTCCTTCGGCCCTTACCATTTCAATCATTCAAATCCGATGGAGAAGCACAAGCGTCGTGTGCTGCTTCATTTCCTCAAAAGATGTGGAAAACCTGTTGGACTGTTTGCAGATGCACTTGCTGAAGTAGTGGAAGATTTGAAGGACTCATACAATCCTCTCGAGCCAGTGTGGAGAGATAATACAGCTAGATTTTTGCAACTCATGTTTCTGGATGGCTGTTTCATGCTTGAAATCTTGAGGACTGCTTCTCATATACTTGATGATTATGCTCCTACTGATCCTATCTTCAGCAAGCAAGGGAAGTTTCATGTCATGCCCTTCATCAAACGCGACATGTTAATGCTCGAGAATCAGTTGCCAATGCTTGTTCTTGTCAAGCTGGTTGCAGTTGAAACTGATAAAGCTAAGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

187

Amino Acids

21.62

Weight (kDa)

6.59

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 30 - 182 3.2e-40 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 114
AccII CGCG 1 cut(s) 480
AclWI GGATC 1 cut(s) 422
AcuI CTGAAG 2 cut(s) 291, 421
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 6 cut(s) 26, 172, 180, 289, 380, 542
AluBI AGCT 3 cut(s) 335, 529, 554
AluI AGCT 3 cut(s) 335, 529, 554
Alw26I GTCTC 1 cut(s) 154
AlwI GGATC 1 cut(s) 422
Ama87I CYCGRG 2 cut(s) 308, 494
AoxI GGCC 2 cut(s) 129, 157
ApeKI GCWGC 1 cut(s) 211
AspS9I GGNCC 1 cut(s) 158
AvaI CYCGRG 2 cut(s) 308, 494
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
BbvI GCAGC 1 cut(s) 198
BccI CCATC 3 cut(s) 43, 181, 356
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 63
BglII AGATCT 1 cut(s) 37
BisI GCNGC 1 cut(s) 212
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 1 cut(s) 213
BmeT110I CYCGRG 2 cut(s) 308, 494
BmgT120I GGNCC 1 cut(s) 158
BmsI GCATC 1 cut(s) 250
Bpu1102I GCTNAGC 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 406
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 20, 314
BseGI GGATG 2 cut(s) 54, 367
BseNI ACTGG 2 cut(s) 20, 314
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 2 cut(s) 131, 159
BsiHKCI CYCGRG 2 cut(s) 308, 494
BsiSI CCGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 154
BsnI GGCC 2 cut(s) 131, 159
BsoBI CYCGRG 2 cut(s) 308, 494
Bsp143I GATC 2 cut(s) 37, 427
Bsp1720I GCTNAGC 1 cut(s) 555
BspANI GGCC 2 cut(s) 131, 159
BspFNI CGCG 1 cut(s) 480
BspPI GGATC 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 20, 314
BssAI RCCGGY 1 cut(s) 104
BssMI GATC 2 cut(s) 37, 427
Bst4CI ACNGT 2 cut(s) 148, 252
BstC8I GCNNGC 2 cut(s) 106, 443
BstDEI CTNAG 1 cut(s) 555
BstF5I GGATG 2 cut(s) 54, 367
BstFNI CGCG 1 cut(s) 480
BstKTI GATC 2 cut(s) 40, 430
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 2 cut(s) 37, 427
BstMWI GCNNNNNNNGC 1 cut(s) 208
BstNSI RCATGY 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 63
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 131, 159
BtsCI GGATG 2 cut(s) 54, 367
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 2 cut(s) 80, 321
Cac8I GCNNGC 2 cut(s) 106, 443
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 191
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 6 cut(s) 10, 352, 373, 458, 463, 484
DdeI CTNAG 1 cut(s) 555
DpnI GATC 2 cut(s) 39, 429
DpnII GATC 2 cut(s) 37, 427
Eco147I AGGCCT 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 291, 421
Eco88I CYCGRG 2 cut(s) 308, 494
FaeI CATG 6 cut(s) 13, 355, 376, 461, 466, 487
FatI CATG 6 cut(s) 9, 351, 372, 457, 462, 483
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 1 cut(s) 212
FokI GGATG 2 cut(s) 61, 374
Fsp4HI GCNGC 1 cut(s) 212
FspBI CTAG 1 cut(s) 336
GluI GCNGC 1 cut(s) 212
HaeIII GGCC 2 cut(s) 131, 159
HapII CCGG 1 cut(s) 105
HgaI GACGC 1 cut(s) 191
Hin1II CATG 6 cut(s) 13, 355, 376, 461, 466, 487
HinfI GANTC 2 cut(s) 293, 499
HpaII CCGG 1 cut(s) 105
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 4 cut(s) 308, 359, 385, 496
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 6 cut(s) 47, 73, 121, 163, 283, 478
HpyCH4III ACNGT 2 cut(s) 148, 252
HpyCH4V TGCA 4 cut(s) 257, 263, 346, 536
HpyF10VI GCNNNNNNNGC 1 cut(s) 208
HpyF3I CTNAG 1 cut(s) 555
Hsp92II CATG 6 cut(s) 13, 355, 376, 461, 466, 487
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 2 cut(s) 37, 427
LmnI GCTCC 2 cut(s) 70, 424
LpnPI CCDG 7 cut(s) 114, 118, 155, 255, 327, 344, 515
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 1 cut(s) 250
MaeI CTAG 1 cut(s) 336
MalI GATC 2 cut(s) 39, 429
MboI GATC 2 cut(s) 37, 427
MboII GAAGA 3 cut(s) 47, 293, 427
MflI RGATCY 1 cut(s) 37
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 4 cut(s) 61, 233, 315, 381
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 3 cut(s) 30, 42, 488
MslI CAYNNNNRTG 1 cut(s) 488
MspI CCGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 1 cut(s) 208
NaeI GCCGGC 1 cut(s) 106
NdeII GATC 2 cut(s) 37, 427
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 6 cut(s) 13, 355, 376, 461, 466, 487
NspI RCATGY 1 cut(s) 487
PaeR7I CTCGAG 2 cut(s) 308, 494
PceI AGGCCT 1 cut(s) 131
PciI ACATGT 1 cut(s) 483
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 499
PkrI GCNGC 1 cut(s) 213
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
PscI ACATGT 1 cut(s) 483
PspPI GGNCC 1 cut(s) 158
PsuI RGATCY 1 cut(s) 37
RseI CAYNNNNRTG 1 cut(s) 488
SaqAI TTAA 3 cut(s) 30, 42, 488
SatI GCNGC 1 cut(s) 212
Sau3AI GATC 2 cut(s) 37, 427
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 287
SetI ASST 5 cut(s) 58, 244, 337, 531, 556
SfaNI GCATC 1 cut(s) 250
SfcI CTRYAG 1 cut(s) 63
Sfr274I CTCGAG 2 cut(s) 308, 494
SlaI CTCGAG 2 cut(s) 308, 494
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 3 cut(s) 308, 385, 494
SmoI CTYRAG 3 cut(s) 308, 385, 494
SseBI AGGCCT 1 cut(s) 131
SspMI CTAG 1 cut(s) 336
StuI AGGCCT 1 cut(s) 131
TaaI ACNGT 2 cut(s) 148, 252
TaqI TCGA 2 cut(s) 309, 495
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 3 cut(s) 30, 42, 488
Tru9I TTAA 3 cut(s) 30, 42, 488
TscAI CASTG 2 cut(s) 80, 321
TseI GCWGC 1 cut(s) 211
TspDTI ATGAA 6 cut(s) 26, 48, 206, 361, 446, 460
TspRI CASTG 2 cut(s) 80, 321
XceI RCATGY 1 cut(s) 487
XhoI CTCGAG 2 cut(s) 308, 494
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.