Rh4DG284200

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002269
Physical Location & Seq
Reverse (-)
50549459 .. 50550078
620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG284200.1

Sequence Viewer

Length: 588 bp
ATGGAGAAGCACAAGCGTCGTGTGCTGCTTCATTTCCTCAAAAGATGTGGAAAACCTCTTGGACTGTTTGCAGATGCACTTGCTGAAGTAGTGGAAGATTTGAAGGACTCATACAATCCTCTCGAGCCAGTGTGGAGAGATAATACAGCTAGATTTTTGCAACTCATGTTTCTGGATGGCTGTTTCATGCTTGAAATCTTGAGGACTGCTTCTCATATACTTGATGATTATGCTCCTACTGATCCTATCTTCAGCAAGCACGGGAAGTTTCATGTCATGCCCTTCATCAAACGCGACATGTTAATGCTCGAGAATCAGTTGCCAATGCTTGTTCTTGTCAAGCTGGTTGCAGTTGAAACTGATAAAGCTAAGGATGAAGAATCTGTGAATAGATTCATAGTAAAGTTCTGCTGCTCCTGCACAGTACCCCTGAACATGGGAAAGTGTGTGCACGTATTGGACGTGTACAGGAAAAGCCTGCTTCAGGAAGATCCCCACCAGAAAATAGTTCGCCACAGAGCATCTGTGAATGGCAATGCGATCATCTGGTCTGCAACAGAGCTTAAAGAAGCCGGAATCAGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

195

Amino Acids

22.51

Weight (kDa)

8.51

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 1 - 195 6.8e-37 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 294
AclWI GGATC 2 cut(s) 236, 485
AcuI CTGAAG 3 cut(s) 105, 235, 467
AfaI GTAC 2 cut(s) 426, 467
AfiI CCNNNNNNNGG 2 cut(s) 436, 484
AflIII ACRYGT 2 cut(s) 297, 462
AgsI TTSAA 3 cut(s) 103, 194, 356
AjiI CACGTC 1 cut(s) 463
AluBI AGCT 4 cut(s) 149, 343, 368, 562
AluI AGCT 4 cut(s) 149, 343, 368, 562
Alw21I GWGCWC 1 cut(s) 453
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 2 cut(s) 236, 485
Ama87I CYCGRG 2 cut(s) 122, 308
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 2 cut(s) 25, 411
Asp700I GAANNNNTTC 1 cut(s) 392
AvaI CYCGRG 2 cut(s) 122, 308
BaeGI GKGCMC 1 cut(s) 453
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 12, 398
BccI CCATC 1 cut(s) 170
BcgI CGANNNNNNTGC 1 cut(s) 33
BfaI CTAG 2 cut(s) 150, 586
BisI GCNGC 2 cut(s) 26, 412
BlsI GCNGC 2 cut(s) 27, 413
BmeT110I CYCGRG 2 cut(s) 122, 308
BmgBI CACGTC 1 cut(s) 463
BmsI GCATC 2 cut(s) 64, 530
Bpu10I CCTNAGC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 220
BsaAI YACGTR 1 cut(s) 454
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 484
Bse1I ACTGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 541
BseGI GGATG 2 cut(s) 181, 379
BseLI CCNNNNNNNGG 2 cut(s) 436, 484
BseMI GCAATG 1 cut(s) 541
BseNI ACTGG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 453
BseXI GCAGC 2 cut(s) 12, 398
BsgI GTGCAG 1 cut(s) 403
Bsh1236I CGCG 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 453
BsiHKCI CYCGRG 2 cut(s) 122, 308
BsiSI CCGG 1 cut(s) 573
BslI CCNNNNNNNGG 2 cut(s) 436, 484
BsoBI CYCGRG 2 cut(s) 122, 308
Bsp1286I GDGCHC 1 cut(s) 453
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 3 cut(s) 241, 490, 540
BspFNI CGCG 1 cut(s) 294
BspPI GGATC 2 cut(s) 236, 485
BsrDI GCAATG 1 cut(s) 541
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 1 cut(s) 128
BssMI GATC 3 cut(s) 241, 490, 540
Bst4CI ACNGT 2 cut(s) 66, 424
BstAUI TGTACA 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 257, 479
BstDEI CTNAG 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 2 cut(s) 181, 379
BstFNI CGCG 1 cut(s) 294
BstKTI GATC 3 cut(s) 244, 493, 543
BstMBI GATC 3 cut(s) 241, 490, 540
BstMWI GCNNNNNNNGC 2 cut(s) 22, 417
BstNSI RCATGY 1 cut(s) 301
BstSLI GKGCMC 1 cut(s) 453
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 12, 398
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 181, 379
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 257, 479
CseI GACGC 1 cut(s) 5
Csp6I GTAC 2 cut(s) 425, 466
CspCI CAANNNNNGTGG 2 cut(s) 28, 63
CviAII CATG 6 cut(s) 166, 187, 272, 277, 298, 436
CviJI RGCY 8 cut(s) 127, 149, 180, 343, 368, 477, 562, 572
CviKI_1 RGCY 8 cut(s) 127, 149, 180, 343, 368, 477, 562, 572
CviQI GTAC 2 cut(s) 425, 466
DdeI CTNAG 1 cut(s) 369
DpnI GATC 3 cut(s) 243, 492, 542
DpnII GATC 3 cut(s) 241, 490, 540
Eco57I CTGAAG 3 cut(s) 105, 235, 467
Eco88I CYCGRG 2 cut(s) 122, 308
EcoNI CCTNNNNNAGG 1 cut(s) 482
FaeI CATG 6 cut(s) 169, 190, 275, 280, 301, 439
FatI CATG 6 cut(s) 165, 186, 271, 276, 297, 435
Fnu4HI GCNGC 2 cut(s) 26, 412
FokI GGATG 2 cut(s) 188, 386
Fsp4HI GCNGC 2 cut(s) 26, 412
FspBI CTAG 2 cut(s) 150, 586
GluI GCNGC 2 cut(s) 26, 412
HapII CCGG 1 cut(s) 573
HgaI GACGC 1 cut(s) 5
Hin1II CATG 6 cut(s) 169, 190, 275, 280, 301, 439
HinfI GANTC 6 cut(s) 107, 313, 380, 393, 576, 582
HpaII CCGG 1 cut(s) 573
Hpy166II GTNNAC 2 cut(s) 451, 466
Hpy188I TCNGA 1 cut(s) 581
Hpy188III TCNNGA 5 cut(s) 122, 173, 199, 310, 485
Hpy8I GTNNAC 2 cut(s) 451, 466
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 2 cut(s) 97, 292
HpyCH4III ACNGT 2 cut(s) 66, 424
HpyCH4IV ACGT 2 cut(s) 453, 462
HpyCH4V TGCA 7 cut(s) 71, 77, 160, 350, 420, 451, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 417
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 2 cut(s) 453, 462
Hsp92II CATG 6 cut(s) 169, 190, 275, 280, 301, 439
Kzo9I GATC 3 cut(s) 241, 490, 540
LmnI GCTCC 2 cut(s) 238, 419
Lsp1109I GCAGC 2 cut(s) 12, 398
LweI GCATC 2 cut(s) 64, 530
MaeI CTAG 2 cut(s) 150, 586
MaeII ACGT 2 cut(s) 453, 462
MalI GATC 3 cut(s) 243, 492, 542
MboI GATC 3 cut(s) 241, 490, 540
MboII GAAGA 4 cut(s) 107, 241, 389, 500
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 1 cut(s) 453
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 4 cut(s) 47, 66, 129, 195
MroXI GAANNNNTTC 1 cut(s) 392
MseI TTAA 2 cut(s) 302, 564
MslI CAYNNNNRTG 1 cut(s) 302
MspI CCGG 1 cut(s) 573
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 22, 417
NdeII GATC 3 cut(s) 241, 490, 540
NlaIII CATG 6 cut(s) 169, 190, 275, 280, 301, 439
NspI RCATGY 1 cut(s) 301
PaeR7I CTCGAG 2 cut(s) 122, 308
PciI ACATGT 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 5 cut(s) 313, 380, 393, 576, 582
PkrI GCNGC 2 cut(s) 27, 413
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 454
PscI ACATGT 1 cut(s) 297
PsuI RGATCY 1 cut(s) 490
RsaI GTAC 2 cut(s) 426, 467
RsaNI GTAC 2 cut(s) 425, 466
RseI CAYNNNNRTG 1 cut(s) 302
SaqAI TTAA 2 cut(s) 302, 564
SatI GCNGC 2 cut(s) 26, 412
Sau3AI GATC 3 cut(s) 241, 490, 540
SchI GAGTC 1 cut(s) 101
SduI GDGCHC 1 cut(s) 453
SetI ASST 7 cut(s) 58, 151, 345, 370, 456, 465, 564
SfaNI GCATC 2 cut(s) 64, 530
Sfr274I CTCGAG 2 cut(s) 122, 308
SlaI CTCGAG 2 cut(s) 122, 308
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 3 cut(s) 122, 199, 308
SmoI CTYRAG 3 cut(s) 122, 199, 308
SspMI CTAG 2 cut(s) 150, 586
TaaI ACNGT 2 cut(s) 66, 424
TaiI ACGT 2 cut(s) 456, 465
TaqI TCGA 2 cut(s) 123, 309
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 5 cut(s) 313, 380, 393, 576, 582
Tru1I TTAA 2 cut(s) 302, 564
Tru9I TTAA 2 cut(s) 302, 564
TscAI CASTG 1 cut(s) 135
TseI GCWGC 2 cut(s) 25, 411
TspDTI ATGAA 6 cut(s) 20, 175, 260, 274, 385, 390
TspRI CASTG 1 cut(s) 135
VneI GTGCAC 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 482
XceI RCATGY 1 cut(s) 301
XhoI CTCGAG 2 cut(s) 122, 308
XmnI GAANNNNTTC 1 cut(s) 392
XspI CTAG 2 cut(s) 150, 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.