Rroxscaffold_5G00369220

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002269
Physical Location & Seq
Forward (+)
51050491 .. 51052009
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369220.1

Sequence Viewer

Length: 1449 bp
ATGGCTGGCACCGGATGGATTATTCAAGTTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAGAAGCATCACTGGAATAAGGGGTCAATCTACAAATTACCAGCCAGCCTCACAGATATGAACAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGGCCTTACCATTATGATCCTTCAAATCCAATGGAGGAGCACAAGAACCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGTCTGTTGAGTTGTTTGTAGATGCATTGGCTGAAGTAAAGAATGATTTGAAGGACTCATATACTCTGCTTCATTCTGTGCCGAAACAAGTCACAGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTGGAAATCCTAAGGACTGCTGCTCATGTACTGGACGATTATGCTCCTAATGATCCCATCTTCGGCAATCATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAACATGATAAAGCTAAGGTAAAGTATGTCAACAATGAGTCTAGAGATGAAGGGTTTGTGACCAAGCTCATACTCAAGTTCCTCTGCTCCTGCAATGTTTATCCAAACATGGGAAAGTGTGTGCATGTATTGGACGTGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCACAAATATATTCGCCACAATATAAGGTTAATGGAGCGTGACGATGAGATCATCTGGTCTGCAACAGCGCTCAATGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTAAAAGACATCACATTCGCCAGGGGAGTGCTGAGGCTCCCTGTTGTTGTTGTGGATGACACCACCGAGTCCATGTTCTTGAATCTGATGGCATTTGAAAGATTCCATGTAGGAGCAGGAAATGAGGTGACTTCTTATTTAATCTTCATGGACAACATCATAGACTATGCTAGGGATGTCTCCCTCTTACACTCGAAAGGGATCATCCATAATAGGCTGGGGAGTGACAAAGCAGTGGCTAATCTGTTCAATTCACTCTCTAAGGATGTGACACAAGACCCGGATAGCAGCCTTCTAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATAGGTGGCGCGCCAATCTTATTCACACTTACTTCAGAAATCCATGGGCTGTTATCTCTTTCGTTGCTGCTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATTCCATATATCCCTATTACTTTCCACCAAATGATTCTTCTCCTTCCACTGTCATTCTTGTTCCGGCAGCTCCTCCTCCTCCAAACCATTCATCATCCGACTCCACCTGCCCATCAACACTAGTGATATCTTTCATTCTTGTTTCCATGGTATGCATGCTTACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

482

Amino Acids

54.98

Weight (kDa)

8.02

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 419 9.5e-113 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1394
Acc36I ACCTGC 1 cut(s) 1394
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 3 cut(s) 213, 215, 1182
AciI CCGC 1 cut(s) 211
AclWI GGATC 3 cut(s) 173, 428, 1040
AcuI CTGAAG 3 cut(s) 297, 686, 1189
AfaI GTAC 2 cut(s) 411, 686
AfeI AGCGCT 1 cut(s) 786
AfiI CCNNNNNNNGG 2 cut(s) 443, 656
AflIII ACRYGT 2 cut(s) 489, 681
AhlI ACTAGT 1 cut(s) 1399
AjiI CACGTC 1 cut(s) 682
AjnI CCWGG 1 cut(s) 851
AjuI GAANNNNNNNTTGG 2 cut(s) 1300, 1332
AluBI AGCT 5 cut(s) 535, 560, 613, 1268, 1349
AluI AGCT 5 cut(s) 535, 560, 613, 1268, 1349
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 160, 1015
AlwI GGATC 3 cut(s) 173, 428, 1040
Aor51HI AGCGCT 1 cut(s) 786
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 5 cut(s) 217, 401, 1119, 1238, 1346
ArsI GACNNNNNNTTYG 2 cut(s) 830, 862
AscI GGCGCGCC 1 cut(s) 1180
AspLEI GCGC 5 cut(s) 215, 217, 787, 1182, 1184
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 1112
AsuHPI GGTGA 1 cut(s) 970
AxyI CCTNAGG 1 cut(s) 392
BanI GGYRCC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 1237
Bbv12I GWGCWC 1 cut(s) 204
BbvCI CCTCAGC 1 cut(s) 863
BbvI GCAGC 5 cut(s) 204, 388, 1131, 1225, 1358
BccI CCATC 5 cut(s) 9, 362, 446, 913, 1399
BceAI ACGGC 1 cut(s) 1150
BciT130I CCWGG 1 cut(s) 853
BclI TGATCA 1 cut(s) 715
BcnI CCSGG 1 cut(s) 1112
BcoDI GTCTC 2 cut(s) 160, 1015
BcuI ACTAGT 1 cut(s) 1399
BfaI CTAG 3 cut(s) 588, 1002, 1400
BfmI CTRYAG 2 cut(s) 1156, 1263
BfoI RGCGCY 1 cut(s) 788
BfuAI ACCTGC 1 cut(s) 1394
BglII AGATCT 1 cut(s) 37
BisI GCNGC 5 cut(s) 218, 402, 1120, 1239, 1347
BlsI GCNGC 5 cut(s) 219, 403, 1121, 1240, 1348
Bme1390I CCNGG 2 cut(s) 853, 1112
BmgBI CACGTC 1 cut(s) 682
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 3 cut(s) 10, 461, 869
BmrFI CCNGG 2 cut(s) 853, 1112
BmsI GCATC 2 cut(s) 82, 256
BpiI GAAGAC 1 cut(s) 1237
Bpu10I CCTNAGC 2 cut(s) 561, 863
BpuEI CTTGAG 3 cut(s) 26, 521, 605
BpuMI CCSGG 1 cut(s) 1112
BsaJI CCNNGG 4 cut(s) 852, 1166, 1214, 1425
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 656
Bse1I ACTGG 3 cut(s) 83, 417, 1279
Bse21I CCTNAGG 1 cut(s) 392
Bse3DI GCAATG 1 cut(s) 646
BseBI CCWGG 1 cut(s) 853
BseDI CCNNGG 4 cut(s) 852, 1166, 1214, 1425
BseGI GGATG 6 cut(s) 20, 892, 1012, 1035, 1102, 1373
BseLI CCNNNNNNNGG 2 cut(s) 443, 656
BseMI GCAATG 1 cut(s) 646
BseMII CTCAG 2 cut(s) 854, 1283
BseNI ACTGG 3 cut(s) 83, 417, 1279
BsePI GCGCGC 2 cut(s) 213, 1180
BseRI GAGGAG 4 cut(s) 212, 1341, 1344, 1347
BseXI GCAGC 5 cut(s) 204, 388, 1131, 1225, 1358
BseYI CCCAGC 1 cut(s) 1048
Bsh1236I CGCG 3 cut(s) 213, 215, 1182
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 3 cut(s) 12, 1112, 1343
BslI CCNNNNNNNGG 2 cut(s) 443, 656
BsmAI GTCTC 2 cut(s) 160, 1015
BsnI GGCC 2 cut(s) 137, 166
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 6 cut(s) 37, 178, 433, 715, 765, 1032
Bsp19I CCATGG 2 cut(s) 1214, 1425
BspACI CCGC 1 cut(s) 211
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 2 cut(s) 855, 1282
BspFNI CGCG 3 cut(s) 213, 215, 1182
BspLI GGNNCC 3 cut(s) 10, 461, 869
BspMAI CTGCAG 1 cut(s) 1160
BspMI ACCTGC 1 cut(s) 1394
BspPI GGATC 3 cut(s) 173, 428, 1040
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 646
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 3 cut(s) 83, 417, 1279
BssECI CCNNGG 4 cut(s) 852, 1166, 1214, 1425
BssHII GCGCGC 2 cut(s) 213, 1180
BssMI GATC 6 cut(s) 37, 178, 433, 715, 765, 1032
BssT1I CCWWGG 2 cut(s) 1214, 1425
Bst2UI CCWGG 1 cut(s) 853
Bst4CI ACNGT 2 cut(s) 154, 1330
Bst6I CTCTTC 1 cut(s) 686
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 5 cut(s) 7, 112, 215, 1182, 1436
BstDEI CTNAG 5 cut(s) 392, 561, 863, 1092, 1269
BstDSI CCRYGG 3 cut(s) 1166, 1214, 1425
BstF5I GGATG 6 cut(s) 20, 892, 1012, 1035, 1102, 1373
BstFNI CGCG 3 cut(s) 213, 215, 1182
BstH2I RGCGCY 1 cut(s) 788
BstHHI GCGC 5 cut(s) 215, 217, 787, 1182, 1184
BstKTI GATC 6 cut(s) 40, 181, 436, 718, 768, 1035
BstMAI GTCTC 2 cut(s) 160, 1015
BstMBI GATC 6 cut(s) 37, 178, 433, 715, 765, 1032
BstNI CCWGG 1 cut(s) 853
BstNSI RCATGY 3 cut(s) 493, 674, 1438
BstSCI CCNGG 2 cut(s) 851, 1110
BstSFI CTRYAG 2 cut(s) 1156, 1263
BstUI CGCG 3 cut(s) 213, 215, 1182
BstV1I GCAGC 5 cut(s) 204, 388, 1131, 1225, 1358
BstV2I GAAGAC 1 cut(s) 1237
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
Bsu36I CCTNAGG 1 cut(s) 392
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 3 cut(s) 1166, 1214, 1425
BtrI CACGTC 1 cut(s) 682
BtsCI GGATG 6 cut(s) 20, 892, 1012, 1035, 1102, 1373
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 3 cut(s) 76, 1071, 1326
BveI ACCTGC 1 cut(s) 1394
Cac8I GCNNGC 5 cut(s) 7, 112, 215, 1182, 1436
CfoI GCGC 5 cut(s) 215, 217, 787, 1182, 1184
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 2 cut(s) 410, 685
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 2 cut(s) 410, 685
DdeI CTNAG 5 cut(s) 392, 561, 863, 1092, 1269
DpnI GATC 6 cut(s) 39, 180, 435, 717, 767, 1034
DpnII GATC 6 cut(s) 37, 178, 433, 715, 765, 1032
Eam1104I CTCTTC 1 cut(s) 686
EarI CTCTTC 1 cut(s) 686
Eco130I CCWWGG 2 cut(s) 1214, 1425
Eco147I AGGCCT 1 cut(s) 137
Eco32I GATATC 1 cut(s) 1407
Eco47III AGCGCT 1 cut(s) 786
Eco57I CTGAAG 3 cut(s) 297, 686, 1189
Eco81I CCTNAGG 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 851
EcoRV GATATC 1 cut(s) 1407
EcoT14I CCWWGG 2 cut(s) 1214, 1425
EcoT22I ATGCAT 2 cut(s) 271, 1436
ErhI CCWWGG 2 cut(s) 1214, 1425
FbaI TGATCA 1 cut(s) 715
Fnu4HI GCNGC 5 cut(s) 218, 402, 1120, 1239, 1347
FokI GGATG 6 cut(s) 27, 899, 1019, 1022, 1109, 1360
Fsp4HI GCNGC 5 cut(s) 218, 402, 1120, 1239, 1347
FspBI CTAG 3 cut(s) 588, 1002, 1400
GlaI GCGC 5 cut(s) 214, 216, 786, 1181, 1183
GluI GCNGC 5 cut(s) 218, 402, 1120, 1239, 1347
GsaI CCCAGC 1 cut(s) 1052
HaeII RGCGCY 1 cut(s) 788
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 3 cut(s) 12, 1112, 1343
HhaI GCGC 5 cut(s) 215, 217, 787, 1182, 1184
Hin6I GCGC 5 cut(s) 213, 215, 785, 1180, 1182
HinP1I GCGC 5 cut(s) 213, 215, 785, 1180, 1182
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HpaII CCGG 3 cut(s) 12, 1112, 1343
HphI GGTGA 1 cut(s) 970
Hpy166II GTNNAC 3 cut(s) 577, 685, 1260
Hpy188I TCNGA 4 cut(s) 918, 1208, 1272, 1378
Hpy188III TCNNGA 5 cut(s) 43, 527, 588, 811, 910
Hpy8I GTNNAC 3 cut(s) 577, 685, 1260
HpyAV CCTTC 8 cut(s) 47, 127, 169, 192, 289, 590, 1133, 1332
HpyCH4III ACNGT 2 cut(s) 154, 1330
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 9 cut(s) 269, 352, 639, 670, 779, 821, 1135, 1158, 1434
HpyF3I CTNAG 5 cut(s) 392, 561, 863, 1092, 1269
HpySE526I ACGT 1 cut(s) 681
HspAI GCGC 5 cut(s) 213, 215, 785, 1180, 1182
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 6 cut(s) 37, 178, 433, 715, 765, 1032
LmnI GCTCC 8 cut(s) 199, 430, 465, 638, 751, 873, 944, 1354
Lsp1109I GCAGC 5 cut(s) 204, 388, 1131, 1225, 1358
LweI GCATC 2 cut(s) 82, 256
MaeI CTAG 3 cut(s) 588, 1002, 1400
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 7 cut(s) 334, 485, 604, 755, 958, 1055, 1099
MalI GATC 6 cut(s) 39, 180, 435, 717, 767, 1034
MboI GATC 6 cut(s) 37, 178, 433, 715, 765, 1032
MboII GAAGA 6 cut(s) 47, 433, 703, 967, 1237, 1308
MfeI CAATTG 1 cut(s) 1444
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 4 cut(s) 101, 1081, 1128, 1444
MlyI GAGTC 5 cut(s) 293, 593, 703, 908, 1373
MmeI TCCRAC 1 cut(s) 1401
Mph1103I ATGCAT 2 cut(s) 271, 1436
MseI TTAA 4 cut(s) 30, 494, 746, 971
MslI CAYNNNNRTG 2 cut(s) 122, 494
MspI CCGG 3 cut(s) 12, 1112, 1343
MspR9I CCNGG 2 cut(s) 853, 1112
MunI CAATTG 1 cut(s) 1444
MvaI CCWGG 1 cut(s) 853
MvnI CGCG 3 cut(s) 213, 215, 1182
NciI CCSGG 1 cut(s) 1112
NcoI CCATGG 2 cut(s) 1214, 1425
NdeII GATC 6 cut(s) 37, 178, 433, 715, 765, 1032
NlaIV GGNNCC 3 cut(s) 10, 461, 869
NmuCI GTSAC 7 cut(s) 334, 485, 604, 755, 958, 1055, 1099
NsiI ATGCAT 2 cut(s) 271, 1436
NspI RCATGY 3 cut(s) 493, 674, 1438
PaeI GCATGC 1 cut(s) 1438
PalAI GGCGCGCC 1 cut(s) 1180
PaqCI CACCTGC 1 cut(s) 1394
PauI GCGCGC 2 cut(s) 213, 1180
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 489
PfeI GAWTC 5 cut(s) 505, 801, 913, 933, 1313
PkrI GCNGC 5 cut(s) 219, 403, 1121, 1240, 1348
PleI GAGTC 5 cut(s) 293, 592, 702, 907, 1373
PpsI GAGTC 5 cut(s) 293, 592, 702, 907, 1373
PscI ACATGT 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 851
PspFI CCCAGC 1 cut(s) 1048
PspGI CCWGG 1 cut(s) 851
PspN4I GGNNCC 3 cut(s) 10, 461, 869
PspPI GGNCC 1 cut(s) 164
PstI CTGCAG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 2 cut(s) 213, 1180
RsaI GTAC 2 cut(s) 411, 686
RsaNI GTAC 2 cut(s) 410, 685
RseI CAYNNNNRTG 2 cut(s) 122, 494
SaqAI TTAA 4 cut(s) 30, 494, 746, 971
SatI GCNGC 5 cut(s) 218, 402, 1120, 1239, 1347
Sau3AI GATC 6 cut(s) 37, 178, 433, 715, 765, 1032
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 5 cut(s) 293, 593, 703, 908, 1373
ScrFI CCNGG 2 cut(s) 853, 1112
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 2 cut(s) 82, 256
SfcI CTRYAG 2 cut(s) 1156, 1263
SgsI GGCGCGCC 1 cut(s) 1180
SmiMI CAYNNNNRTG 2 cut(s) 122, 494
SmlI CTYRAG 3 cut(s) 41, 500, 620
SmoI CTYRAG 3 cut(s) 41, 500, 620
SpeI ACTAGT 1 cut(s) 1399
SphI GCATGC 1 cut(s) 1438
Sse9I AATT 4 cut(s) 101, 1081, 1128, 1444
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 1 cut(s) 211
SspMI CTAG 3 cut(s) 588, 1002, 1400
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 851, 1110
StyI CCWWGG 2 cut(s) 1214, 1425
TaaI ACNGT 2 cut(s) 154, 1330
TaiI ACGT 1 cut(s) 684
TaqI TCGA 1 cut(s) 1025
TasI AATT 4 cut(s) 101, 1081, 1128, 1444
TatI WGTACW 2 cut(s) 409, 684
TfiI GAWTC 5 cut(s) 505, 801, 913, 933, 1313
Tru1I TTAA 4 cut(s) 30, 494, 746, 971
Tru9I TTAA 4 cut(s) 30, 494, 746, 971
TscAI CASTG 3 cut(s) 83, 1071, 1333
TseFI GTSAC 7 cut(s) 334, 485, 604, 755, 958, 1055, 1099
TseI GCWGC 5 cut(s) 217, 401, 1119, 1238, 1346
Tsp45I GTSAC 7 cut(s) 334, 485, 604, 755, 958, 1055, 1099
TspRI CASTG 3 cut(s) 83, 1071, 1333
XbaI TCTAGA 1 cut(s) 587
XceI RCATGY 3 cut(s) 493, 674, 1438
XspI CTAG 3 cut(s) 588, 1002, 1400
Zsp2I ATGCAT 2 cut(s) 271, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.