MD16G1130500.v1.1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002269
Physical Location & Seq
Forward (+)
9986038 .. 9987805
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1130500.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGCAGACACGAAATGGATTATTCAAGTTAACGAAGAGCTCGATAAGATGCAGGATCTTTCGATAGAGAAGGAGCATTGGAGCAAGGGGTCGATATACAGACTGCCAACCTGCATCACGGACATTAACAAGAAGGCCTACAAGCCCCAGGTGGTCTCCTTCGGGCCTTACCATTTCAATAATTTAAATCCAAATGAGGACCACAAGCGCAATAATTTAAATCCAATGGAGGAGCACAAGCGCCGCGCCCTTGTCCATTTCCTGAAAAGATGTGGGAAACCTGTTGAGCTGTTCGTCAACGCGCTGGCTGAAGTGGTGCAGGATTTGAAGGACTCGTACAATCCGCTTGAGCCTGAGTGGAGAGATGATACAGATAGATTCTTGCAGCTGATGATTCTGGATGGCTGTTTCATGCTGGAAGTCTTGCGGGTTGCTTCTCATTTACTGGATGATTACGCTCCCAATGATCCCGTCTTCAGCAACCACGGGAAGCTCCATACGATGCCGTACATCAAGCGCGACATGTTGATGCTTGAGAATCAGTTGCCAATCCTGGTTCTTGACAAGCTGGTTGCGGTTGAGAGCGATTACGCTAAGGATGAAGGGTTTGTCATCAAGCTCCTAATCAAGTTCTTCTCCCCCGGTACAGCAGCCTCAATTACGGGAAAATGCGTGCACGCATTGGATGTGTATAGGAAGAGCCAGCTTCTGGGAGAGCCCTATTGCAAAACAGGTCACTGCAGACCAGAGACTGTGAATCGTCATGAGGAAATCATCTGGTCAGCGACAGAGCTTAATGAAGCCGGGATCAGATTCAAGGAAAGCAAAACCACTAGCCTCAAAGACATCTCATTCTCCAGGGGAATCCTCAGGCTCCCATCCATCATTGTGGATGACACCACCGAATCCATGTTCTTGAATCTGATAGCGTTTGAGCGCTTCCATGTTGGGGCAGGCAACGAGGTCACATCCTATATATTTTTCATGGACAATATCATAGACAATGCCAGAGATGTTGCCCTCCTACACTCAAAAGGTATCATCCATAATGCCCTGGGAAGCGACAAGGCAGTTGCGGATCTTTTCAACTCGCTCTCTAAGGACATAACGCTTGATCCAGAAAGCAGCCTTGATGCGGTGCATAAGAATGTGTATCACTACTGCAAGAAGCCTTGGAACGTCTGGCGCGCCAATCTCATGCACACTTATTTCAGAAATCCATGGGCTATTATTTCCCTCGTTGCCGGTGTCTTCCTCTTCGTGCTCACTATAGTTCAGACCGGATACTCCATATATCCCTACTACTTTCCAAATGGTGATTCTCCCACCTGTGCATGTTCTGTTAACAATTCTACTAGCCCTGCGCCTCCTCCTCCGCCTCCACCAAACGCTGCATACTCCACCTCACCATCAAGATTTATATTTTCTTTCGTTCTTATGTCGGTTGTATGGATGCTTACGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

489

Amino Acids

55.53

Weight (kDa)

6.19

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 422 2.7e-116 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
AccB7I CCANNNNNTGG 1 cut(s) 709
AccII CGCG 4 cut(s) 246, 302, 519, 1188
AciI CCGC 7 cut(s) 244, 344, 427, 575, 1076, 1136, 1376
AclWI GGATC 5 cut(s) 63, 461, 815, 1086, 1109
AcuI CTGAAG 2 cut(s) 330, 460
AfaI GTAC 3 cut(s) 338, 509, 646
AfeI AGCGCT 1 cut(s) 938
AfiI CCNNNNNNNGG 3 cut(s) 709, 949, 1135
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 6 cut(s) 26, 178, 328, 817, 919, 1087
AjnI CCWGG 4 cut(s) 147, 552, 857, 1053
AjuI GAANNNNNNNTTGG 2 cut(s) 1303, 1335
AluBI AGCT 8 cut(s) 40, 289, 388, 493, 568, 619, 706, 793
AluI AGCT 8 cut(s) 40, 289, 388, 493, 568, 619, 706, 793
Alw21I GWGCWC 4 cut(s) 42, 237, 678, 1266
Alw26I GTCTC 2 cut(s) 160, 743
Alw44I GTGCAC 1 cut(s) 674
AlwI GGATC 5 cut(s) 63, 461, 815, 1086, 1109
AlwNI CAGNNNCTG 2 cut(s) 709, 752
Aor51HI AGCGCT 1 cut(s) 938
AoxI GGCC 2 cut(s) 135, 164
ApaLI GTGCAC 1 cut(s) 674
ApeKI GCWGC 4 cut(s) 385, 650, 1125, 1391
AscI GGCGCGCC 1 cut(s) 1186
AspLEI GCGC 9 cut(s) 210, 243, 248, 304, 519, 939, 1188, 1190, 1366
AspS9I GGNCC 2 cut(s) 164, 199
AsuC2I CCSGG 2 cut(s) 642, 805
AsuHPI GGTGA 2 cut(s) 1328, 1398
AvaII GGWCC 1 cut(s) 199
AxyI CCTNAGG 1 cut(s) 869
BaeGI GKGCMC 1 cut(s) 678
BanII GRGCYC 2 cut(s) 42, 720
BarI GAAGNNNNNNTAC 2 cut(s) 320, 352
BbsI GAAGAC 2 cut(s) 466, 1243
Bbv12I GWGCWC 4 cut(s) 42, 237, 678, 1266
BbvI GCAGC 4 cut(s) 397, 662, 1137, 1378
BccI CCATC 4 cut(s) 395, 886, 890, 1417
BceAI ACGGC 1 cut(s) 490
BciT130I CCWGG 4 cut(s) 149, 554, 859, 1055
BciVI GTATCC 1 cut(s) 1277
BcnI CCSGG 2 cut(s) 642, 805
BcoDI GTCTC 2 cut(s) 160, 743
BfaI CTAG 2 cut(s) 834, 1356
BfmI CTRYAG 2 cut(s) 739, 1269
BfoI RGCGCY 2 cut(s) 244, 940
BfuAI ACCTGC 1 cut(s) 119
BfuI GTATCC 1 cut(s) 1277
BisI GCNGC 5 cut(s) 244, 386, 651, 1126, 1392
BlsI GCNGC 5 cut(s) 245, 387, 652, 1127, 1393
Bme1390I CCNGG 6 cut(s) 149, 554, 642, 805, 859, 1055
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 164, 199
BmiI GGNNCC 1 cut(s) 875
BmrFI CCNGG 6 cut(s) 149, 554, 642, 805, 859, 1055
BmsI GCATC 6 cut(s) 39, 123, 492, 519, 1123, 1443
BpiI GAAGAC 2 cut(s) 466, 1243
BpmI CTGGAG 1 cut(s) 841
Bpu10I CCTNAGC 1 cut(s) 594
BpuEI CTTGAG 2 cut(s) 368, 554
BpuMI CCSGG 2 cut(s) 642, 805
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 8 cut(s) 147, 484, 640, 858, 1053, 1054, 1172, 1220
BsaWI WCCGGW 1 cut(s) 1280
Bsc4I CCNNNNNNNGG 3 cut(s) 709, 949, 1135
Bse118I RCCGGY 1 cut(s) 1244
Bse1I ACTGG 1 cut(s) 450
Bse21I CCTNAGG 1 cut(s) 869
BseBI CCWGG 4 cut(s) 149, 554, 859, 1055
BseDI CCNNGG 8 cut(s) 147, 484, 640, 858, 1053, 1054, 1172, 1220
BseGI GGATG 9 cut(s) 406, 454, 604, 691, 878, 898, 968, 1041, 1458
BseLI CCNNNNNNNGG 3 cut(s) 709, 949, 1135
BseMII CTCAG 2 cut(s) 345, 883
BseNI ACTGG 1 cut(s) 450
BsePI GCGCGC 1 cut(s) 1186
BseRI GAGGAG 3 cut(s) 245, 1359, 1362
BseSI GKGCMC 1 cut(s) 678
BseXI GCAGC 4 cut(s) 397, 662, 1137, 1378
BsgI GTGCAG 1 cut(s) 338
Bsh1236I CGCG 4 cut(s) 246, 302, 519, 1188
BshFI GGCC 2 cut(s) 137, 166
BsiHKAI GWGCWC 4 cut(s) 42, 237, 678, 1266
BsiSI CCGG 4 cut(s) 642, 804, 1245, 1281
BslI CCNNNNNNNGG 3 cut(s) 709, 949, 1135
BsmAI GTCTC 2 cut(s) 160, 743
BsnI GGCC 2 cut(s) 137, 166
Bso31I GGTCTC 1 cut(s) 160
Bsp1286I GDGCHC 5 cut(s) 42, 237, 678, 720, 1266
Bsp143I GATC 5 cut(s) 55, 466, 807, 1078, 1114
Bsp19I CCATGG 1 cut(s) 1220
BspACI CCGC 7 cut(s) 244, 344, 427, 575, 1076, 1136, 1376
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 2 cut(s) 346, 882
BspFNI CGCG 4 cut(s) 246, 302, 519, 1188
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 1 cut(s) 875
BspMAI CTGCAG 1 cut(s) 743
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 5 cut(s) 63, 461, 815, 1086, 1109
BspQI GCTCTTC 2 cut(s) 30, 692
BspTNI GGTCTC 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 1244
BsrI ACTGG 1 cut(s) 450
BssAI RCCGGY 1 cut(s) 1244
BssECI CCNNGG 8 cut(s) 147, 484, 640, 858, 1053, 1054, 1172, 1220
BssHII GCGCGC 1 cut(s) 1186
BssMI GATC 5 cut(s) 55, 466, 807, 1078, 1114
BssT1I CCWWGG 2 cut(s) 1172, 1220
Bst2UI CCWGG 4 cut(s) 149, 554, 859, 1055
Bst4CI ACNGT 1 cut(s) 754
Bst6I CTCTTC 3 cut(s) 30, 692, 1262
BstC8I GCNNGC 6 cut(s) 306, 674, 678, 704, 955, 1188
BstDEI CTNAG 4 cut(s) 354, 594, 869, 1098
BstDSI CCRYGG 2 cut(s) 484, 1220
BstF5I GGATG 9 cut(s) 406, 454, 604, 691, 878, 898, 968, 1041, 1458
BstFNI CGCG 4 cut(s) 246, 302, 519, 1188
BstH2I RGCGCY 2 cut(s) 244, 940
BstHHI GCGC 9 cut(s) 210, 243, 248, 304, 519, 939, 1188, 1190, 1366
BstKTI GATC 5 cut(s) 58, 469, 810, 1081, 1117
BstMAI GTCTC 2 cut(s) 160, 743
BstMBI GATC 5 cut(s) 55, 466, 807, 1078, 1114
BstNI CCWGG 4 cut(s) 149, 554, 859, 1055
BstNSI RCATGY 2 cut(s) 526, 1338
BstSCI CCNGG 6 cut(s) 147, 552, 640, 803, 857, 1053
BstSFI CTRYAG 2 cut(s) 739, 1269
BstSLI GKGCMC 1 cut(s) 678
BstUI CGCG 4 cut(s) 246, 302, 519, 1188
BstV1I GCAGC 4 cut(s) 397, 662, 1137, 1378
BstV2I GAAGAC 2 cut(s) 466, 1243
BstX2I RGATCY 2 cut(s) 55, 1078
BstXI CCANNNNNNTGG 1 cut(s) 889
BstYI RGATCY 2 cut(s) 55, 1078
Bsu36I CCTNAGG 1 cut(s) 869
BsuI GTATCC 1 cut(s) 1277
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 2 cut(s) 484, 1220
BtsCI GGATG 9 cut(s) 406, 454, 604, 691, 878, 898, 968, 1041, 1458
BtsI GCAGTG 1 cut(s) 736
BtsIMutI CAGTG 1 cut(s) 736
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 6 cut(s) 306, 674, 678, 704, 955, 1188
CaiI CAGNNNCTG 2 cut(s) 709, 752
CciI TCATGA 1 cut(s) 763
CfoI GCGC 9 cut(s) 210, 243, 248, 304, 519, 939, 1188, 1190, 1366
Cfr10I RCCGGY 1 cut(s) 1244
Cfr13I GGNCC 2 cut(s) 164, 199
Csp6I GTAC 3 cut(s) 337, 508, 645
CviAII CATG 9 cut(s) 412, 523, 764, 910, 944, 985, 1198, 1221, 1335
CviQI GTAC 3 cut(s) 337, 508, 645
DdeI CTNAG 4 cut(s) 354, 594, 869, 1098
DpnI GATC 5 cut(s) 57, 468, 809, 1080, 1116
DpnII GATC 5 cut(s) 55, 466, 807, 1078, 1114
DraI TTTAAA 2 cut(s) 186, 219
Eam1104I CTCTTC 3 cut(s) 30, 692, 1262
EarI CTCTTC 3 cut(s) 30, 692, 1262
EciI GGCGGA 1 cut(s) 1365
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 2 cut(s) 1172, 1220
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 2 cut(s) 42, 720
Eco31I GGTCTC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 199
Eco47III AGCGCT 1 cut(s) 938
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 2 cut(s) 330, 460
Eco81I CCTNAGG 1 cut(s) 869
EcoICRI GAGCTC 1 cut(s) 40
EcoRII CCWGG 4 cut(s) 147, 552, 857, 1053
EcoT14I CCWWGG 2 cut(s) 1172, 1220
EcoT38I GRGCYC 2 cut(s) 42, 720
ErhI CCWWGG 2 cut(s) 1172, 1220
FaeI CATG 9 cut(s) 415, 526, 767, 913, 947, 988, 1201, 1224, 1338
FatI CATG 9 cut(s) 411, 522, 763, 909, 943, 984, 1197, 1220, 1334
FauI CCCGC 1 cut(s) 420
Fnu4HI GCNGC 5 cut(s) 244, 386, 651, 1126, 1392
FokI GGATG 8 cut(s) 413, 461, 611, 698, 865, 905, 955, 1028
FriOI GRGCYC 2 cut(s) 42, 720
Fsp4HI GCNGC 5 cut(s) 244, 386, 651, 1126, 1392
FspBI CTAG 2 cut(s) 834, 1356
GlaI GCGC 9 cut(s) 209, 242, 247, 303, 518, 938, 1187, 1189, 1365
GluI GCNGC 5 cut(s) 244, 386, 651, 1126, 1392
GsuI CTGGAG 1 cut(s) 841
HaeII RGCGCY 2 cut(s) 244, 940
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 4 cut(s) 642, 804, 1245, 1281
HhaI GCGC 9 cut(s) 210, 243, 248, 304, 519, 939, 1188, 1190, 1366
Hin1II CATG 9 cut(s) 415, 526, 767, 913, 947, 988, 1201, 1224, 1338
Hin6I GCGC 9 cut(s) 208, 241, 246, 302, 517, 937, 1186, 1188, 1364
HinP1I GCGC 9 cut(s) 208, 241, 246, 302, 517, 937, 1186, 1188, 1364
HincII GTYRAC 3 cut(s) 31, 298, 1345
HindII GTYRAC 3 cut(s) 31, 298, 1345
HpaI GTTAAC 2 cut(s) 31, 1345
HpaII CCGG 4 cut(s) 642, 804, 1245, 1281
HphI GGTGA 2 cut(s) 1328, 1398
Hpy166II GTNNAC 4 cut(s) 31, 298, 676, 1345
Hpy188I TCNGA 4 cut(s) 812, 924, 1214, 1278
Hpy188III TCNNGA 7 cut(s) 262, 398, 560, 764, 916, 1118, 1413
Hpy8I GTNNAC 4 cut(s) 31, 298, 676, 1345
HpyAV CCTTC 5 cut(s) 64, 127, 169, 322, 596
HpyCH4III ACNGT 1 cut(s) 754
HpyCH4IV ACGT 1 cut(s) 1179
HpyF3I CTNAG 4 cut(s) 354, 594, 869, 1098
HpySE526I ACGT 1 cut(s) 1179
Hsp92II CATG 9 cut(s) 415, 526, 767, 913, 947, 988, 1201, 1224, 1338
HspAI GCGC 9 cut(s) 208, 241, 246, 302, 517, 937, 1186, 1188, 1364
KspAI GTTAAC 2 cut(s) 31, 1345
Kzo9I GATC 5 cut(s) 55, 466, 807, 1078, 1114
LguI GCTCTTC 2 cut(s) 30, 692
LmnI GCTCC 7 cut(s) 73, 81, 232, 463, 498, 624, 879
Lsp1109I GCAGC 4 cut(s) 397, 662, 1137, 1378
LweI GCATC 6 cut(s) 39, 123, 492, 519, 1123, 1443
MaeI CTAG 2 cut(s) 834, 1356
MaeII ACGT 1 cut(s) 1179
MaeIII GTNAC 2 cut(s) 734, 964
MalI GATC 5 cut(s) 57, 468, 809, 1080, 1116
MboI GATC 5 cut(s) 55, 466, 807, 1078, 1114
MboII GAAGA 6 cut(s) 47, 466, 625, 709, 1243, 1249
MflI RGATCY 2 cut(s) 55, 1078
MhlI GDGCHC 5 cut(s) 42, 237, 678, 720, 1266
MluCI AATT 4 cut(s) 181, 214, 657, 1348
MlyI GAGTC 1 cut(s) 326
MseI TTAA 6 cut(s) 30, 126, 185, 218, 795, 1344
MslI CAYNNNNRTG 3 cut(s) 527, 887, 1146
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 4 cut(s) 642, 804, 1245, 1281
MspR9I CCNGG 6 cut(s) 149, 554, 642, 805, 859, 1055
MteI GCGCNGCGC 1 cut(s) 244
MvaI CCWGG 4 cut(s) 149, 554, 859, 1055
MvnI CGCG 4 cut(s) 246, 302, 519, 1188
NciI CCSGG 2 cut(s) 642, 805
NcoI CCATGG 1 cut(s) 1220
NdeII GATC 5 cut(s) 55, 466, 807, 1078, 1114
NlaIII CATG 9 cut(s) 415, 526, 767, 913, 947, 988, 1201, 1224, 1338
NlaIV GGNNCC 1 cut(s) 875
NmuCI GTSAC 2 cut(s) 734, 964
NspI RCATGY 2 cut(s) 526, 1338
PagI TCATGA 1 cut(s) 763
PalAI GGCGCGCC 1 cut(s) 1186
PasI CCCWGGG 1 cut(s) 1054
PauI GCGCGC 1 cut(s) 1186
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 522
PciSI GCTCTTC 2 cut(s) 30, 692
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 9 cut(s) 378, 394, 538, 757, 813, 864, 905, 919, 1319
PflMI CCANNNNNTGG 1 cut(s) 709
PkrI GCNGC 5 cut(s) 245, 387, 652, 1127, 1393
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
PscI ACATGT 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 4 cut(s) 147, 552, 857, 1053
PspGI CCWGG 4 cut(s) 147, 552, 857, 1053
PspN4I GGNNCC 1 cut(s) 875
PspPI GGNCC 2 cut(s) 164, 199
PstI CTGCAG 1 cut(s) 743
PstNI CAGNNNCTG 2 cut(s) 709, 752
PsuI RGATCY 2 cut(s) 55, 1078
PteI GCGCGC 1 cut(s) 1186
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 3 cut(s) 338, 509, 646
RsaNI GTAC 3 cut(s) 337, 508, 645
RseI CAYNNNNRTG 3 cut(s) 527, 887, 1146
SacI GAGCTC 1 cut(s) 42
SapI GCTCTTC 2 cut(s) 30, 692
SaqAI TTAA 6 cut(s) 30, 126, 185, 218, 795, 1344
SatI GCNGC 5 cut(s) 244, 386, 651, 1126, 1392
Sau3AI GATC 5 cut(s) 55, 466, 807, 1078, 1114
Sau96I GGNCC 2 cut(s) 164, 199
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 6 cut(s) 149, 554, 642, 805, 859, 1055
SduI GDGCHC 5 cut(s) 42, 237, 678, 720, 1266
SfaNI GCATC 6 cut(s) 39, 123, 492, 519, 1123, 1443
SfcI CTRYAG 2 cut(s) 739, 1269
SgsI GGCGCGCC 1 cut(s) 1186
SinI GGWCC 1 cut(s) 199
SmiI ATTTAAAT 2 cut(s) 186, 219
SmiMI CAYNNNNRTG 3 cut(s) 527, 887, 1146
SmlI CTYRAG 2 cut(s) 347, 533
SmoI CTYRAG 2 cut(s) 347, 533
Sse9I AATT 4 cut(s) 181, 214, 657, 1348
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 7 cut(s) 244, 344, 427, 575, 1076, 1136, 1376
SspMI CTAG 2 cut(s) 834, 1356
SstI GAGCTC 1 cut(s) 42
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 6 cut(s) 147, 552, 640, 803, 857, 1053
StyI CCWWGG 2 cut(s) 1172, 1220
SwaI ATTTAAAT 2 cut(s) 186, 219
TaaI ACNGT 1 cut(s) 754
TaiI ACGT 1 cut(s) 1182
TaqI TCGA 3 cut(s) 42, 62, 92
TasI AATT 4 cut(s) 181, 214, 657, 1348
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 9 cut(s) 378, 394, 538, 757, 813, 864, 905, 919, 1319
Tru1I TTAA 6 cut(s) 30, 126, 185, 218, 795, 1344
Tru9I TTAA 6 cut(s) 30, 126, 185, 218, 795, 1344
TscAI CASTG 1 cut(s) 743
TseFI GTSAC 2 cut(s) 734, 964
TseI GCWGC 4 cut(s) 385, 650, 1125, 1391
Tsp45I GTSAC 2 cut(s) 734, 964
TspDTI ATGAA 4 cut(s) 400, 615, 813, 973
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 1 cut(s) 743
Van91I CCANNNNNTGG 1 cut(s) 709
VneI GTGCAC 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 199
XceI RCATGY 2 cut(s) 526, 1338
XspI CTAG 2 cut(s) 834, 1356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.