Rh4AG281300

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002269
Physical Location & Seq
Reverse (-)
59649271 .. 59650190
920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG281300.1

Sequence Viewer

Length: 789 bp
ATGGGCAAGTGTGTGCATGTGTTGGACATGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCCCAAATATATTCGCTACAAAATAAGGTCAAGTGGGCATGACGATGAGATCATCTGGTCCGCAACAGCGCTCAATGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCGCCCGGGGAGTGTTGAGGCTCCCTGTTGTTGTTGTGGATGACACCACCGAGTCCATGTTCTTGAATCTGATGGCGTTTGAAAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGTCAACATCATAGACAATGCTATGGATGTTGCCCTCTTACACTCGAAAGGGATCATCCAGAATGCTTTGGGGAGTGACAAGGCAGTGGCTAATCTGTTCAATTCACTGTGTAAGGATGTGACACAGGACCCGGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATGAGTGGCGTGCTAATCTTATTCACACTTATTTCCGGAATCCATGGGCTATTATCTCTTTAATTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATGCCATATATCCCTATTACCATCCACCAAAGGATACTTCTCCTTCCACTGTCATTCTCGTTCCGGCTCCTCCTCCAAAACATTCATCCTCCGACTCCACCTCCCCATCGACACTTGTGATTTCTTCCATCCTTGTTACCATGGTATGCATGCTGACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

262

Amino Acids

29.44

Weight (kDa)

8.59

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 3 - 201 3.6e-65 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 552
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 56, 386
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 1 cut(s) 32
AfeI AGCGCT 1 cut(s) 132
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 5 cut(s) 157, 259, 275, 427, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 646, 678
AluBI AGCT 1 cut(s) 614
AluI AGCT 1 cut(s) 614
AlwI GGATC 2 cut(s) 56, 386
Ama87I CYCGRG 1 cut(s) 198
Aor13HI TCCGGA 1 cut(s) 552
Aor51HI AGCGCT 1 cut(s) 132
ApeKI GCWGC 1 cut(s) 465
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AspLEI GCGC 1 cut(s) 133
AspS9I GGNCC 2 cut(s) 120, 454
AsuC2I CCSGG 3 cut(s) 199, 200, 458
AsuHPI GGTGA 1 cut(s) 316
AvaI CYCGRG 1 cut(s) 198
AvaII GGWCC 2 cut(s) 120, 454
BbsI GAAGAC 1 cut(s) 583
BbvI GCAGC 1 cut(s) 477
BccI CCATC 4 cut(s) 259, 654, 739, 761
BceAI ACGGC 1 cut(s) 496
BciVI GTATCC 1 cut(s) 652
BcnI CCSGG 3 cut(s) 199, 200, 458
BfmI CTRYAG 2 cut(s) 502, 609
BfoI RGCGCY 1 cut(s) 134
BfuI GTATCC 1 cut(s) 652
BisI GCNGC 1 cut(s) 466
BlsI GCNGC 1 cut(s) 467
Bme1390I CCNGG 3 cut(s) 199, 200, 458
Bme18I GGWCC 2 cut(s) 120, 454
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 2 cut(s) 120, 454
BmiI GGNNCC 3 cut(s) 215, 456, 693
BmrFI CCNGG 3 cut(s) 199, 200, 458
BpiI GAAGAC 1 cut(s) 583
BpuMI CCSGG 3 cut(s) 199, 200, 458
BsaJI CCNNGG 5 cut(s) 198, 199, 512, 560, 765
BsaWI WCCGGW 1 cut(s) 552
BsaXI ACNNNNNCTCC 2 cut(s) 710, 740
Bse1I ACTGG 1 cut(s) 625
BseAI TCCGGA 1 cut(s) 552
BseDI CCNNGG 5 cut(s) 198, 199, 512, 560, 765
BseGI GGATG 7 cut(s) 238, 358, 381, 448, 646, 710, 753
BseMII CTCAG 1 cut(s) 629
BseNI ACTGG 1 cut(s) 625
BseRI GAGGAG 2 cut(s) 684, 687
BseXI GCAGC 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 4 cut(s) 199, 458, 553, 689
BsmI GAATGC 1 cut(s) 394
BsoBI CYCGRG 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 552
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 3 cut(s) 61, 111, 378
Bsp19I CCATGG 2 cut(s) 560, 765
BspACI CCGC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 628
BspEI TCCGGA 1 cut(s) 552
BspLI GGNNCC 3 cut(s) 215, 456, 693
BspMAI CTGCAG 1 cut(s) 506
BspPI GGATC 2 cut(s) 56, 386
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 1 cut(s) 625
BssECI CCNNGG 5 cut(s) 198, 199, 512, 560, 765
BssMI GATC 3 cut(s) 61, 111, 378
BssT1I CCWWGG 2 cut(s) 560, 765
Bst4CI ACNGT 2 cut(s) 435, 676
Bst6I CTCTTC 2 cut(s) 32, 326
BstAUI TGTACA 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 528, 776
BstDEI CTNAG 1 cut(s) 615
BstDSI CCRYGG 3 cut(s) 512, 560, 765
BstF5I GGATG 7 cut(s) 238, 358, 381, 448, 646, 710, 753
BstH2I RGCGCY 1 cut(s) 134
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 3 cut(s) 64, 114, 381
BstMBI GATC 3 cut(s) 61, 111, 378
BstNSI RCATGY 3 cut(s) 20, 31, 778
BstSCI CCNGG 3 cut(s) 197, 198, 456
BstSFI CTRYAG 2 cut(s) 502, 609
BstV1I GCAGC 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 583
BsuI GTATCC 1 cut(s) 652
BtgI CCRYGG 3 cut(s) 512, 560, 765
BtsCI GGATG 7 cut(s) 238, 358, 381, 448, 646, 710, 753
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 3 cut(s) 417, 431, 672
Cac8I GCNNGC 2 cut(s) 528, 776
CfoI GCGC 1 cut(s) 133
Cfr13I GGNCC 2 cut(s) 120, 454
Cfr9I CCCGGG 1 cut(s) 198
Csp6I GTAC 1 cut(s) 31
CviJI RGCY 9 cut(s) 58, 177, 214, 416, 468, 511, 566, 614, 692
CviKI_1 RGCY 9 cut(s) 58, 177, 214, 416, 468, 511, 566, 614, 692
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 615
DpnI GATC 3 cut(s) 63, 113, 380
DpnII GATC 3 cut(s) 61, 111, 378
Eam1104I CTCTTC 2 cut(s) 32, 326
EarI CTCTTC 2 cut(s) 32, 326
Eco130I CCWWGG 2 cut(s) 560, 765
Eco47I GGWCC 2 cut(s) 120, 454
Eco47III AGCGCT 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 454
EcoT14I CCWWGG 2 cut(s) 560, 765
EcoT22I ATGCAT 1 cut(s) 776
ErhI CCWWGG 2 cut(s) 560, 765
Fnu4HI GCNGC 1 cut(s) 466
FokI GGATG 7 cut(s) 245, 365, 368, 455, 633, 697, 740
Fsp4HI GCNGC 1 cut(s) 466
GlaI GCGC 1 cut(s) 132
GluI GCNGC 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 134
HapII CCGG 4 cut(s) 199, 458, 553, 689
HhaI GCGC 1 cut(s) 133
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 7 cut(s) 40, 147, 245, 259, 279, 556, 719
HpaII CCGG 4 cut(s) 199, 458, 553, 689
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 2 cut(s) 331, 606
Hpy188I TCNGA 3 cut(s) 264, 618, 718
Hpy188III TCNNGA 4 cut(s) 157, 256, 385, 553
Hpy8I GTNNAC 2 cut(s) 331, 606
HpyAV CCTTC 2 cut(s) 479, 678
HpyCH4III ACNGT 2 cut(s) 435, 676
HpyCH4V TGCA 5 cut(s) 16, 167, 481, 504, 774
HpyF3I CTNAG 1 cut(s) 615
HspAI GCGC 1 cut(s) 131
Kpn2I TCCGGA 1 cut(s) 552
Kzo9I GATC 3 cut(s) 61, 111, 378
LmnI GCTCC 3 cut(s) 219, 290, 697
Lsp1109I GCAGC 1 cut(s) 477
MaeIII GTNAC 4 cut(s) 304, 401, 445, 760
MalI GATC 3 cut(s) 63, 113, 380
MboI GATC 3 cut(s) 61, 111, 378
MboII GAAGA 4 cut(s) 49, 313, 583, 741
MfeI CAATTG 1 cut(s) 784
MluCI AATT 4 cut(s) 427, 474, 579, 784
MlyI GAGTC 3 cut(s) 49, 254, 713
MmeI TCCRAC 1 cut(s) 741
Mph1103I ATGCAT 1 cut(s) 776
MroI TCCGGA 1 cut(s) 552
MseI TTAA 1 cut(s) 578
MslI CAYNNNNRTG 1 cut(s) 105
MspI CCGG 4 cut(s) 199, 458, 553, 689
MspR9I CCNGG 3 cut(s) 199, 200, 458
MunI CAATTG 1 cut(s) 784
Mva1269I GAATGC 1 cut(s) 394
NciI CCSGG 3 cut(s) 199, 200, 458
NcoI CCATGG 2 cut(s) 560, 765
NdeII GATC 3 cut(s) 61, 111, 378
NlaIV GGNNCC 3 cut(s) 215, 456, 693
NmuCI GTSAC 3 cut(s) 304, 401, 445
NsiI ATGCAT 1 cut(s) 776
NspI RCATGY 3 cut(s) 20, 31, 778
PaeI GCATGC 1 cut(s) 778
PciI ACATGT 1 cut(s) 27
PctI GAATGC 1 cut(s) 394
PfeI GAWTC 4 cut(s) 147, 259, 279, 556
PkrI GCNGC 1 cut(s) 467
PleI GAGTC 3 cut(s) 48, 253, 713
PpsI GAGTC 3 cut(s) 48, 253, 713
PpuMI RGGWCCY 1 cut(s) 454
PscI ACATGT 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 454
PspN4I GGNNCC 3 cut(s) 215, 456, 693
PspPI GGNCC 2 cut(s) 120, 454
PspPPI RGGWCCY 1 cut(s) 454
PstI CTGCAG 1 cut(s) 506
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 105
SaqAI TTAA 1 cut(s) 578
SatI GCNGC 1 cut(s) 466
Sau3AI GATC 3 cut(s) 61, 111, 378
Sau96I GGNCC 2 cut(s) 120, 454
SchI GAGTC 3 cut(s) 49, 254, 713
ScrFI CCNGG 3 cut(s) 199, 200, 458
SetI ASST 5 cut(s) 92, 155, 306, 616, 728
SfcI CTRYAG 2 cut(s) 502, 609
SinI GGWCC 2 cut(s) 120, 454
SmaI CCCGGG 1 cut(s) 200
SmiMI CAYNNNNRTG 1 cut(s) 105
SphI GCATGC 1 cut(s) 778
Sse9I AATT 4 cut(s) 427, 474, 579, 784
SsiI CCGC 1 cut(s) 123
StyD4I CCNGG 3 cut(s) 197, 198, 456
StyI CCWWGG 2 cut(s) 560, 765
TaaI ACNGT 2 cut(s) 435, 676
TaqI TCGA 2 cut(s) 371, 734
TasI AATT 4 cut(s) 427, 474, 579, 784
TatI WGTACW 1 cut(s) 30
TfiI GAWTC 4 cut(s) 147, 259, 279, 556
Tru1I TTAA 1 cut(s) 578
Tru9I TTAA 1 cut(s) 578
TscAI CASTG 3 cut(s) 417, 438, 679
TseFI GTSAC 3 cut(s) 304, 401, 445
TseI GCWGC 1 cut(s) 465
Tsp45I GTSAC 3 cut(s) 304, 401, 445
TspDTI ATGAA 3 cut(s) 153, 313, 699
TspMI CCCGGG 1 cut(s) 198
TspRI CASTG 3 cut(s) 417, 438, 679
VpaK11BI GGWCC 2 cut(s) 120, 454
XceI RCATGY 3 cut(s) 20, 31, 778
XmaI CCCGGG 1 cut(s) 198
Zsp2I ATGCAT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.