Rh4DG284100

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002269
Physical Location & Seq
Reverse (-)
50548907 .. 50549395
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG284100.1

Sequence Viewer

Length: 489 bp
ATGGTTGTGGATGATTCAACCGAGTCCACGTTCTTGAATATGACAGCATTTGAGAGATTCCATGTTGGAGCAGGAAATGAGGTGACGTCTTACATATTCTTCATGGACAGCATCATAGACAATCACAGAGATGTTGGCCTCCTGCAATCAAAAGGCATCATCCACAACTTTCTGGGAAGTGATGATGCCGTGGCTGAACTTTTGAACTCACTCTCCAAGGATATAACTCTTGACCCACACTGCAGCCTTGATAAAGTTCATAAAAGTGTCTATCACGACTGCAAGAAGCCTTGGAATAACGAGTGGCGCGCTAATCTTATCCAGACTTATTTCAGAAATCCATGGGCTATTATATCTCTTTTTGCAGCAATCTTGCTCTTTGTGCTTACTATAGCTCAGACTGTTACCCAGGTTATGGATGAAATAGCTCCCTCCTCTTCAGAACCGGGGTCTCCTCCTCCACTTAAGGTGTCTTCAAATCGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

162

Amino Acids

18.1

Weight (kDa)

5.2

Isoelectric Point (pI)

29.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 2 - 129 1.4e-42 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 415
AccII CGCG 1 cut(s) 309
AcuI CTGAAG 1 cut(s) 423
AcyI GRCGYC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 415
AflII CTTAAG 1 cut(s) 464
AgsI TTSAA 4 cut(s) 18, 37, 205, 477
AjnI CCWGG 1 cut(s) 408
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 2 cut(s) 395, 428
AluI AGCT 2 cut(s) 395, 428
Alw26I GTCTC 1 cut(s) 456
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 2 cut(s) 243, 365
AspLEI GCGC 2 cut(s) 309, 311
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 465
BbvI GCAGC 2 cut(s) 255, 377
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 410
BcnI CCSGG 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 456
BfmI CTRYAG 2 cut(s) 241, 390
BfrI CTTAAG 1 cut(s) 464
BisI GCNGC 2 cut(s) 244, 366
BlsI GCNGC 2 cut(s) 245, 367
Bme1390I CCNGG 2 cut(s) 410, 447
BmrFI CCNGG 2 cut(s) 410, 447
BmsI GCATC 3 cut(s) 120, 165, 175
BpiI GAAGAC 1 cut(s) 465
BpuMI CCSGG 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 6 cut(s) 189, 216, 290, 341, 408, 446
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 410
BseDI CCNNGG 6 cut(s) 189, 216, 290, 341, 408, 446
BseGI GGATG 3 cut(s) 16, 159, 424
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 410
BsePI GCGCGC 1 cut(s) 307
BseRI GAGGAG 3 cut(s) 424, 444, 447
BseXI GCAGC 2 cut(s) 255, 377
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 1 cut(s) 138
BsiSI CCGG 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 1 cut(s) 456
BsnI GGCC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 456
Bsp19I CCATGG 1 cut(s) 341
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 1 cut(s) 309
BspMAI CTGCAG 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 456
BssECI CCNNGG 6 cut(s) 189, 216, 290, 341, 408, 446
BssHII GCGCGC 1 cut(s) 307
BssNI GRCGYC 1 cut(s) 86
BssT1I CCWWGG 3 cut(s) 216, 290, 341
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 1 cut(s) 442
BstACI GRCGYC 1 cut(s) 86
BstAFI CTTAAG 1 cut(s) 464
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 2 cut(s) 189, 341
BstF5I GGATG 3 cut(s) 16, 159, 424
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 2 cut(s) 309, 311
BstMAI GTCTC 1 cut(s) 456
BstMWI GCNNNNNNNGC 1 cut(s) 382
BstNI CCWGG 1 cut(s) 410
BstSCI CCNGG 2 cut(s) 408, 445
BstSFI CTRYAG 2 cut(s) 241, 390
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 255, 377
BstV2I GAAGAC 1 cut(s) 465
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 2 cut(s) 189, 341
BtsCI GGATG 3 cut(s) 16, 159, 424
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 2 cut(s) 309, 311
CviAII CATG 3 cut(s) 62, 103, 342
CviJI RGCY 7 cut(s) 138, 194, 246, 289, 347, 395, 428
CviKI_1 RGCY 7 cut(s) 138, 194, 246, 289, 347, 395, 428
DdeI CTNAG 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 442
EarI CTCTTC 1 cut(s) 442
Eco130I CCWWGG 3 cut(s) 216, 290, 341
Eco31I GGTCTC 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 408
EcoT14I CCWWGG 3 cut(s) 216, 290, 341
ErhI CCWWGG 3 cut(s) 216, 290, 341
FaeI CATG 3 cut(s) 65, 106, 345
FatI CATG 3 cut(s) 61, 102, 341
Fnu4HI GCNGC 2 cut(s) 244, 366
FokI GGATG 3 cut(s) 23, 146, 431
Fsp4HI GCNGC 2 cut(s) 244, 366
GlaI GCGC 2 cut(s) 308, 310
GluI GCNGC 2 cut(s) 244, 366
HaeIII GGCC 1 cut(s) 138
HapII CCGG 1 cut(s) 446
HhaI GCGC 2 cut(s) 309, 311
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 3 cut(s) 65, 106, 345
Hin6I GCGC 2 cut(s) 307, 309
HinP1I GCGC 2 cut(s) 307, 309
HinfI GANTC 3 cut(s) 14, 23, 57
HpaII CCGG 1 cut(s) 446
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 3 cut(s) 335, 399, 442
Hpy188III TCNNGA 4 cut(s) 34, 230, 275, 322
Hpy8I GTNNAC 1 cut(s) 27
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 2 cut(s) 29, 86
HpyCH4V TGCA 5 cut(s) 145, 243, 282, 365, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 382
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 2 cut(s) 29, 86
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 3 cut(s) 65, 106, 345
HspAI GCGC 2 cut(s) 307, 309
LmnI GCTCC 2 cut(s) 68, 433
LpnPI CCDG 7 cut(s) 57, 155, 158, 335, 395, 422, 459
Lsp1109I GCAGC 2 cut(s) 255, 377
LweI GCATC 3 cut(s) 120, 165, 175
MaeII ACGT 2 cut(s) 29, 86
MaeIII GTNAC 2 cut(s) 82, 403
MboII GAAGA 3 cut(s) 91, 429, 465
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 6 cut(s) 73, 149, 442, 445, 465, 468
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 2 cut(s) 129, 264
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 1 cut(s) 446
MspR9I CCNGG 2 cut(s) 410, 447
MvaI CCWGG 1 cut(s) 410
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 382
NciI CCSGG 1 cut(s) 447
NcoI CCATGG 1 cut(s) 341
NlaIII CATG 3 cut(s) 65, 106, 345
NmuCI GTSAC 1 cut(s) 82
PauI GCGCGC 1 cut(s) 307
PfeI GAWTC 2 cut(s) 14, 57
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 2 cut(s) 245, 367
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 408
PspGI CCWGG 1 cut(s) 408
PstI CTGCAG 1 cut(s) 245
PteI GCGCGC 1 cut(s) 307
RseI CAYNNNNRTG 2 cut(s) 129, 264
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 2 cut(s) 244, 366
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 2 cut(s) 410, 447
SetI ASST 7 cut(s) 32, 84, 89, 397, 414, 430, 471
SfaNI GCATC 3 cut(s) 120, 165, 175
SfcI CTRYAG 2 cut(s) 241, 390
SmiMI CAYNNNNRTG 2 cut(s) 129, 264
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
StyD4I CCNGG 2 cut(s) 408, 445
StyI CCWWGG 3 cut(s) 216, 290, 341
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 2 cut(s) 32, 89
TfiI GAWTC 2 cut(s) 14, 57
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 245
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 2 cut(s) 243, 365
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 3 cut(s) 91, 248, 435
TspRI CASTG 1 cut(s) 245
Van91I CCANNNNNTGG 1 cut(s) 415
Vha464I CTTAAG 1 cut(s) 464
ZraI GACGTC 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.