Rh4DG284000

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002269
Physical Location & Seq
Reverse (-)
50546396 .. 50547525
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG284000.1

Sequence Viewer

Length: 1038 bp
ATGCTGGAAATCATAAGGAGTGCTGCTCATGTATTGGATGATTATGCTCCTAATGATCCCATCTTCGGAGAACATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAACATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTAATAGTCAAGTTCCTCTGCTCCAGTAACGTTTCTCCAAACATGGGCAAGTGTGTGCATGTGTTGGACATGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCCCAAATTTATTCGCTACAAAATAAGGTCAAGTGAGCATGACGATGAGATCATCTGGTCCGCAACAGCGCTCAATGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCGCCCGGGGAGTGTTGAGGCTCCCTGTTGTTGTTGTGGATGACACCACCGAGTCCATGTTCTTGAATCTGATGGCGTTTGAAAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCTATGGATGTTGCCCTCTTACACTCGAAAGGGATTATCCAGAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTCTTCAATTCACTGTCTAAGGATGTGACACAGGACCCAGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATGAGTGGCGCGCCAATCTTATTCACACTTACTTCCGAAATCCATGGGCTATTATCTCTTTCATTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGTTATGCCATATATCCCTATTACCATCCACCAAATGATTCTTCTCCTTCCACTGTCATTCTCGTTCCGGCTCCTCCTCCAAAACATTCATCCTCCAACTCCACCTCCCCATCGACACTTGTGATTTCTTTCATCCTCGTTACCATGGTATGCATGCTGACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

345

Amino Acids

38.95

Weight (kDa)

6.7

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 2 - 284 6.5e-82 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 777
AciI CCGC 1 cut(s) 372
AclI AACGTT 1 cut(s) 237
AclWI GGATC 2 cut(s) 50, 305
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 281
AfaI GTAC 1 cut(s) 281
AfeI AGCGCT 1 cut(s) 381
AfiI CCNNNNNNNGG 2 cut(s) 65, 251
AflIII ACRYGT 2 cut(s) 111, 276
AgsI TTSAA 7 cut(s) 152, 170, 406, 508, 524, 676, 722
AjuI GAANNNNNNNTTGG 2 cut(s) 895, 927
AluBI AGCT 4 cut(s) 157, 182, 208, 863
AluI AGCT 4 cut(s) 157, 182, 208, 863
AlwI GGATC 2 cut(s) 50, 305
Ama87I CYCGRG 1 cut(s) 447
Aor51HI AGCGCT 1 cut(s) 381
ApeKI GCWGC 2 cut(s) 23, 714
ApoI RAATTY 1 cut(s) 317
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AscI GGCGCGCC 1 cut(s) 775
AspLEI GCGC 3 cut(s) 382, 777, 779
AspS9I GGNCC 2 cut(s) 369, 703
AsuC2I CCSGG 2 cut(s) 448, 449
AsuHPI GGTGA 1 cut(s) 565
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 2 cut(s) 369, 703
BbsI GAAGAC 1 cut(s) 832
BbvI GCAGC 2 cut(s) 10, 726
BccI CCATC 4 cut(s) 68, 508, 903, 988
BceAI ACGGC 1 cut(s) 745
BcnI CCSGG 2 cut(s) 448, 449
BfmI CTRYAG 2 cut(s) 751, 858
BfoI RGCGCY 1 cut(s) 383
BisI GCNGC 2 cut(s) 24, 715
BlsI GCNGC 2 cut(s) 25, 716
Bme1390I CCNGG 2 cut(s) 448, 449
Bme18I GGWCC 2 cut(s) 369, 703
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 2 cut(s) 369, 703
BmiI GGNNCC 4 cut(s) 83, 464, 705, 942
BmrFI CCNGG 2 cut(s) 448, 449
BpiI GAAGAC 1 cut(s) 832
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 2 cut(s) 448, 449
BsaJI CCNNGG 5 cut(s) 447, 448, 761, 809, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 251
Bse1I ACTGG 2 cut(s) 231, 874
Bse3DI GCAATG 1 cut(s) 828
BseDI CCNNGG 5 cut(s) 447, 448, 761, 809, 1014
BseGI GGATG 8 cut(s) 43, 193, 487, 607, 697, 895, 959, 1002
BseLI CCNNNNNNNGG 2 cut(s) 65, 251
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 1 cut(s) 878
BseNI ACTGG 2 cut(s) 231, 874
BsePI GCGCGC 1 cut(s) 775
BseRI GAGGAG 2 cut(s) 933, 936
BseXI GCAGC 2 cut(s) 10, 726
Bsh1236I CGCG 1 cut(s) 777
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 2 cut(s) 448, 938
BslI CCNNNNNNNGG 2 cut(s) 65, 251
BsmI GAATGC 1 cut(s) 643
BsoBI CYCGRG 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 3 cut(s) 55, 310, 360
Bsp19I CCATGG 2 cut(s) 809, 1014
BspACI CCGC 1 cut(s) 372
BspCNI CTCAG 1 cut(s) 877
BspFNI CGCG 1 cut(s) 777
BspLI GGNNCC 4 cut(s) 83, 464, 705, 942
BspMAI CTGCAG 1 cut(s) 755
BspPI GGATC 2 cut(s) 50, 305
BsrDI GCAATG 1 cut(s) 828
BsrGI TGTACA 1 cut(s) 279
BsrI ACTGG 2 cut(s) 231, 874
BssECI CCNNGG 5 cut(s) 447, 448, 761, 809, 1014
BssHII GCGCGC 1 cut(s) 775
BssMI GATC 3 cut(s) 55, 310, 360
BssT1I CCWWGG 2 cut(s) 809, 1014
Bst4CI ACNGT 2 cut(s) 684, 925
Bst6I CTCTTC 3 cut(s) 281, 575, 677
BstAUI TGTACA 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 777, 1025
BstDEI CTNAG 3 cut(s) 183, 687, 864
BstDSI CCRYGG 3 cut(s) 761, 809, 1014
BstF5I GGATG 8 cut(s) 43, 193, 487, 607, 697, 895, 959, 1002
BstFNI CGCG 1 cut(s) 777
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 3 cut(s) 382, 777, 779
BstKTI GATC 3 cut(s) 58, 313, 363
BstMBI GATC 3 cut(s) 55, 310, 360
BstNSI RCATGY 4 cut(s) 115, 269, 280, 1027
BstSCI CCNGG 2 cut(s) 446, 447
BstSFI CTRYAG 2 cut(s) 751, 858
BstUI CGCG 1 cut(s) 777
BstV1I GCAGC 2 cut(s) 10, 726
BstV2I GAAGAC 1 cut(s) 832
BtgI CCRYGG 3 cut(s) 761, 809, 1014
BtsCI GGATG 8 cut(s) 43, 193, 487, 607, 697, 895, 959, 1002
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 666, 680, 921
Cac8I GCNNGC 2 cut(s) 777, 1025
CfoI GCGC 3 cut(s) 382, 777, 779
Cfr13I GGNCC 2 cut(s) 369, 703
Cfr9I CCCGGG 1 cut(s) 447
Csp6I GTAC 1 cut(s) 280
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 3 cut(s) 183, 687, 864
DpnI GATC 3 cut(s) 57, 312, 362
DpnII GATC 3 cut(s) 55, 310, 360
Eam1104I CTCTTC 3 cut(s) 281, 575, 677
EarI CTCTTC 3 cut(s) 281, 575, 677
Eco130I CCWWGG 2 cut(s) 809, 1014
Eco47I GGWCC 2 cut(s) 369, 703
Eco47III AGCGCT 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 703
EcoT14I CCWWGG 2 cut(s) 809, 1014
EcoT22I ATGCAT 1 cut(s) 1025
ErhI CCWWGG 2 cut(s) 809, 1014
Fnu4HI GCNGC 2 cut(s) 24, 715
FokI GGATG 8 cut(s) 50, 200, 494, 614, 704, 882, 946, 989
Fsp4HI GCNGC 2 cut(s) 24, 715
GlaI GCGC 3 cut(s) 381, 776, 778
GluI GCNGC 2 cut(s) 24, 715
GsuI CTGGAG 1 cut(s) 214
HaeII RGCGCY 1 cut(s) 383
HapII CCGG 2 cut(s) 448, 938
HhaI GCGC 3 cut(s) 382, 777, 779
Hin6I GCGC 3 cut(s) 380, 775, 777
HinP1I GCGC 3 cut(s) 380, 775, 777
HinfI GANTC 7 cut(s) 127, 289, 396, 494, 508, 528, 908
HpaII CCGG 2 cut(s) 448, 938
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 1 cut(s) 855
Hpy188I TCNGA 4 cut(s) 68, 513, 803, 867
Hpy188III TCNNGA 4 cut(s) 149, 406, 505, 634
Hpy8I GTNNAC 1 cut(s) 855
HpyAV CCTTC 3 cut(s) 185, 728, 927
HpyCH4III ACNGT 2 cut(s) 684, 925
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 5 cut(s) 265, 416, 730, 753, 1023
HpyF3I CTNAG 3 cut(s) 183, 687, 864
HpySE526I ACGT 1 cut(s) 237
HspAI GCGC 3 cut(s) 380, 775, 777
Kzo9I GATC 3 cut(s) 55, 310, 360
LmnI GCTCC 6 cut(s) 52, 87, 233, 468, 539, 946
Lsp1109I GCAGC 2 cut(s) 10, 726
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 7 cut(s) 107, 199, 233, 553, 650, 694, 1009
MalI GATC 3 cut(s) 57, 312, 362
MboI GATC 3 cut(s) 55, 310, 360
MboII GAAGA 6 cut(s) 55, 298, 562, 664, 832, 903
MfeI CAATTG 1 cut(s) 1033
MluCI AATT 4 cut(s) 317, 676, 723, 1033
MlyI GAGTC 2 cut(s) 298, 503
MmeI TCCRAC 2 cut(s) 252, 990
Mph1103I ATGCAT 1 cut(s) 1025
MseI TTAA 1 cut(s) 116
MslI CAYNNNNRTG 2 cut(s) 116, 354
MspI CCGG 2 cut(s) 448, 938
MspR9I CCNGG 2 cut(s) 448, 449
MunI CAATTG 1 cut(s) 1033
Mva1269I GAATGC 1 cut(s) 643
MvnI CGCG 1 cut(s) 777
NciI CCSGG 2 cut(s) 448, 449
NcoI CCATGG 2 cut(s) 809, 1014
NdeII GATC 3 cut(s) 55, 310, 360
NlaIV GGNNCC 4 cut(s) 83, 464, 705, 942
NmuCI GTSAC 5 cut(s) 107, 199, 553, 650, 694
NsiI ATGCAT 1 cut(s) 1025
NspI RCATGY 4 cut(s) 115, 269, 280, 1027
PaeI GCATGC 1 cut(s) 1027
PalAI GGCGCGCC 1 cut(s) 775
PauI GCGCGC 1 cut(s) 775
PciI ACATGT 2 cut(s) 111, 276
PctI GAATGC 1 cut(s) 643
PfeI GAWTC 5 cut(s) 127, 396, 508, 528, 908
PkrI GCNGC 2 cut(s) 25, 716
PleI GAGTC 2 cut(s) 297, 502
PpsI GAGTC 2 cut(s) 297, 502
PpuMI RGGWCCY 1 cut(s) 703
PscI ACATGT 2 cut(s) 111, 276
Psp1406I AACGTT 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 703
PspN4I GGNNCC 4 cut(s) 83, 464, 705, 942
PspPI GGNCC 2 cut(s) 369, 703
PspPPI RGGWCCY 1 cut(s) 703
PstI CTGCAG 1 cut(s) 755
PteI GCGCGC 1 cut(s) 775
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 2 cut(s) 116, 354
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 2 cut(s) 24, 715
Sau3AI GATC 3 cut(s) 55, 310, 360
Sau96I GGNCC 2 cut(s) 369, 703
SchI GAGTC 2 cut(s) 298, 503
ScrFI CCNGG 2 cut(s) 448, 449
SetI ASST 9 cut(s) 159, 184, 210, 240, 341, 404, 555, 865, 977
SfcI CTRYAG 2 cut(s) 751, 858
SgsI GGCGCGCC 1 cut(s) 775
SinI GGWCC 2 cut(s) 369, 703
SmaI CCCGGG 1 cut(s) 449
SmiMI CAYNNNNRTG 2 cut(s) 116, 354
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SphI GCATGC 1 cut(s) 1027
Sse9I AATT 4 cut(s) 317, 676, 723, 1033
SsiI CCGC 1 cut(s) 372
StyD4I CCNGG 2 cut(s) 446, 447
StyI CCWWGG 2 cut(s) 809, 1014
TaaI ACNGT 2 cut(s) 684, 925
TaiI ACGT 1 cut(s) 240
TaqI TCGA 2 cut(s) 620, 983
TasI AATT 4 cut(s) 317, 676, 723, 1033
TatI WGTACW 1 cut(s) 279
TfiI GAWTC 5 cut(s) 127, 396, 508, 528, 908
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 3 cut(s) 666, 687, 928
TseFI GTSAC 5 cut(s) 107, 199, 553, 650, 694
TseI GCWGC 2 cut(s) 23, 714
Tsp45I GTSAC 5 cut(s) 107, 199, 553, 650, 694
TspDTI ATGAA 6 cut(s) 204, 402, 562, 817, 948, 991
TspMI CCCGGG 1 cut(s) 447
TspRI CASTG 3 cut(s) 666, 687, 928
VpaK11BI GGWCC 2 cut(s) 369, 703
XapI RAATTY 1 cut(s) 317
XceI RCATGY 4 cut(s) 115, 269, 280, 1027
XmaI CCCGGG 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.