RchiOBHm_Chr4g0427561

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002269
Physical Location & Seq
Forward (+)
52784184 .. 52784798
615 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39648

Sequence Viewer

Length: 606 bp
ATGGCTGGCACCGGATGGATTATTCAAGTTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAGAAGCATCACTGGAATAAGGGGTCAATCTACAAATTACCAGCCAGCCTCACAGACATGAACAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTCGGGCCTTACCATTATGATCCTTCAAATCCAATGGAGGAGCACAAGCATCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGTCTGTTGAGCTGTTTGTAGATGCATTGGCTGAAGTAGAGAATGATTTGAAGGACTCATATACTCTGCTTCATTCTGTGCCGAAAGAAGTTACAGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTTGAAATTTTAAGGACTGCTGCTCATGTACAGCTGGAAGATTATGCTCCTAATGATCCCATCTTCGGCAATCATGGCAGGCTCCATGTTGTGCCATACATCAAGCGTGACATGTTAATGCTAGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAGCATGATAAAGCTAAGGTAAAGTATGTCAACGATGAGTCCAGAGTAATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

201

Amino Acids

23.11

Weight (kDa)

5.98

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 187 5.6e-44 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 2 cut(s) 213, 215
AclWI GGATC 2 cut(s) 173, 431
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 6 cut(s) 26, 63, 186, 295, 386, 533
AluBI AGCT 4 cut(s) 256, 415, 538, 563
AluI AGCT 4 cut(s) 256, 415, 538, 563
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 2 cut(s) 173, 431
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 2 cut(s) 217, 401
ApoI RAATTY 1 cut(s) 387
AspLEI GCGC 2 cut(s) 215, 217
AspS9I GGNCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 204, 388
BccI CCATC 3 cut(s) 9, 362, 449
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 503
BglII AGATCT 1 cut(s) 37
BisI GCNGC 2 cut(s) 218, 402
BlsI GCNGC 2 cut(s) 219, 403
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 10, 464
BmsI GCATC 3 cut(s) 82, 217, 256
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 26
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse1I ACTGG 1 cut(s) 83
BseGI GGATG 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 446
BseNI ACTGG 1 cut(s) 83
BsePI GCGCGC 1 cut(s) 213
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 2 cut(s) 204, 388
Bsh1236I CGCG 2 cut(s) 213, 215
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 446
BsmAI GTCTC 1 cut(s) 160
BsnI GGCC 2 cut(s) 137, 166
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 409
Bsp143I GATC 3 cut(s) 37, 178, 436
BspANI GGCC 2 cut(s) 137, 166
BspFNI CGCG 2 cut(s) 213, 215
BspLI GGNNCC 2 cut(s) 10, 464
BspPI GGATC 2 cut(s) 173, 431
BspT107I GGYRCC 1 cut(s) 8
BsrGI TGTACA 1 cut(s) 409
BsrI ACTGG 1 cut(s) 83
BssHII GCGCGC 1 cut(s) 213
BssMI GATC 3 cut(s) 37, 178, 436
Bst4CI ACNGT 1 cut(s) 154
BstAUI TGTACA 1 cut(s) 409
BstC8I GCNNGC 4 cut(s) 7, 112, 215, 461
BstDEI CTNAG 1 cut(s) 564
BstF5I GGATG 1 cut(s) 20
BstFNI CGCG 2 cut(s) 213, 215
BstHHI GCGC 2 cut(s) 215, 217
BstKTI GATC 3 cut(s) 40, 181, 439
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 3 cut(s) 37, 178, 436
BstMWI GCNNNNNNNGC 2 cut(s) 214, 456
BstNSI RCATGY 1 cut(s) 496
BstUI CGCG 2 cut(s) 213, 215
BstV1I GCAGC 2 cut(s) 204, 388
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 137, 166
BtgZI GCGATG 1 cut(s) 194
BtsCI GGATG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 76
Cac8I GCNNGC 4 cut(s) 7, 112, 215, 461
CfoI GCGC 2 cut(s) 215, 217
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 1 cut(s) 410
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviAII CATG 8 cut(s) 49, 124, 379, 407, 455, 467, 493, 554
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 1 cut(s) 564
DpnI GATC 3 cut(s) 39, 180, 438
DpnII GATC 3 cut(s) 37, 178, 436
Eco147I AGGCCT 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 297
EcoT22I ATGCAT 1 cut(s) 271
FaeI CATG 8 cut(s) 52, 127, 382, 410, 458, 470, 496, 557
FatI CATG 8 cut(s) 48, 123, 378, 406, 454, 466, 492, 553
Fnu4HI GCNGC 2 cut(s) 218, 402
FokI GGATG 1 cut(s) 27
Fsp4HI GCNGC 2 cut(s) 218, 402
FspBI CTAG 1 cut(s) 503
GlaI GCGC 2 cut(s) 214, 216
GluI GCNGC 2 cut(s) 218, 402
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 1 cut(s) 12
HhaI GCGC 2 cut(s) 215, 217
Hin1II CATG 8 cut(s) 52, 127, 382, 410, 458, 470, 496, 557
Hin6I GCGC 2 cut(s) 213, 215
HinP1I GCGC 2 cut(s) 213, 215
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 3 cut(s) 299, 508, 587
HpaII CCGG 1 cut(s) 12
Hpy166II GTNNAC 1 cut(s) 580
Hpy188III TCNNGA 3 cut(s) 43, 530, 591
Hpy8I GTNNAC 1 cut(s) 580
HpyAV CCTTC 5 cut(s) 47, 127, 169, 192, 289
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 269, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 456
HpyF3I CTNAG 1 cut(s) 564
Hsp92II CATG 8 cut(s) 52, 127, 382, 410, 458, 470, 496, 557
HspAI GCGC 2 cut(s) 213, 215
Kzo9I GATC 3 cut(s) 37, 178, 436
LmnI GCTCC 3 cut(s) 199, 433, 468
LpnPI CCDG 8 cut(s) 25, 64, 120, 124, 362, 401, 445, 524
Lsp1109I GCAGC 2 cut(s) 204, 388
LweI GCATC 3 cut(s) 82, 217, 256
MaeI CTAG 1 cut(s) 503
MaeIII GTNAC 2 cut(s) 334, 488
MalI GATC 3 cut(s) 39, 180, 438
MboI GATC 3 cut(s) 37, 178, 436
MboII GAAGA 3 cut(s) 47, 431, 436
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 3 cut(s) 101, 387, 597
MlyI GAGTC 2 cut(s) 293, 596
MnlI CCTC 4 cut(s) 125, 156, 190, 239
Mph1103I ATGCAT 1 cut(s) 271
MseI TTAA 3 cut(s) 30, 392, 497
MslI CAYNNNNRTG 2 cut(s) 122, 497
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 1 cut(s) 12
MvnI CGCG 2 cut(s) 213, 215
MwoI GCNNNNNNNGC 2 cut(s) 214, 456
NdeII GATC 3 cut(s) 37, 178, 436
NlaIII CATG 8 cut(s) 52, 127, 382, 410, 458, 470, 496, 557
NlaIV GGNNCC 2 cut(s) 10, 464
NmuCI GTSAC 1 cut(s) 488
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 1 cut(s) 496
PauI GCGCGC 1 cut(s) 213
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 492
PfeI GAWTC 1 cut(s) 508
PkrI GCNGC 2 cut(s) 219, 403
PleI GAGTC 2 cut(s) 293, 595
PpsI GAGTC 2 cut(s) 293, 595
PscI ACATGT 1 cut(s) 492
PspN4I GGNNCC 2 cut(s) 10, 464
PspPI GGNCC 1 cut(s) 164
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 2 cut(s) 122, 497
SaqAI TTAA 3 cut(s) 30, 392, 497
SatI GCNGC 2 cut(s) 218, 402
Sau3AI GATC 3 cut(s) 37, 178, 436
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 2 cut(s) 293, 596
SduI GDGCHC 1 cut(s) 204
SetI ASST 6 cut(s) 58, 258, 417, 540, 565, 570
SfaNI GCATC 3 cut(s) 82, 217, 256
SmiMI CAYNNNNRTG 2 cut(s) 122, 497
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 3 cut(s) 101, 387, 597
SseBI AGGCCT 1 cut(s) 137
SspMI CTAG 1 cut(s) 503
StuI AGGCCT 1 cut(s) 137
TaaI ACNGT 1 cut(s) 154
TasI AATT 3 cut(s) 101, 387, 597
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 1 cut(s) 508
Tru1I TTAA 3 cut(s) 30, 392, 497
Tru9I TTAA 3 cut(s) 30, 392, 497
TscAI CASTG 1 cut(s) 83
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 2 cut(s) 217, 401
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 5 cut(s) 48, 140, 212, 305, 367
TspRI CASTG 1 cut(s) 83
XapI RAATTY 1 cut(s) 387
XceI RCATGY 1 cut(s) 496
XspI CTAG 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.