FvH4_4g34930

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001597
Physical Location & Seq
Forward (+)
32439985 .. 32441866
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34930.t1

Sequence Viewer

Length: 546 bp
ATGGATGGCAGCAATGCAGCAGCAGCGGAGGAACTGGCCGTTGGGTGCTTCGTCTCCATCAAGACCACCTTGGGCGACGACTTCCAGGGCCAGGTCATCACCTTTGACCGCCCCTCCAACATCCTCATCCTTCAAGAGGGTTCCAAAGGAGGGCCGAAGCGAAACATCAGGCTACTCAAGGCCAACTTTATCAAGGAGTTGAGCTATTTGGGTCAAGGTGAAGACCCACTTGACATTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCAGAGAGGAGTCAGCTATCAGGCAAGCAGAGGCAGACTGTGAGAGGATTGGTGTGGGTGTCACTGGCCAGGCTCAGAACATTTTCGATGCCTTGTCTAAGACGCTTCCAGTACGCTGGGACAAGACTGTCATAGTTGTGATGAATGAGGTTCGTGTGAGCAGTCCATATCTTCCGGAGTCTGTCAGTGGAGGAACTCCTGCTGCCAATGATCGGGTGAAGAAAGTGCTTGAGTTGGAGAGGAGGAGGTTGCAAACTCGTGGTGGGGGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

182

Amino Acids

19.79

Weight (kDa)

7.66

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM12_LSM PF21166 10 - 66 2.5e-14 LSM12, LSM domain
AD PF09793 84 - 166 1.4e-25 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 2 cut(s) 26, 109
AcoI YGGCCR 2 cut(s) 36, 337
AfaI GTAC 1 cut(s) 384
AfiI CCNNNNNNNGG 4 cut(s) 91, 136, 150, 483
AgsI TTSAA 1 cut(s) 134
AjnI CCWGG 3 cut(s) 84, 90, 339
AjuI GAANNNNNNNTTGG 2 cut(s) 24, 56
AluBI AGCT 2 cut(s) 204, 287
AluI AGCT 2 cut(s) 204, 287
Alw26I GTCTC 2 cut(s) 58, 269
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 5 cut(s) 36, 88, 152, 180, 337
ApeKI GCWGC 5 cut(s) 9, 17, 20, 23, 473
Asp700I GAANNNNTTC 1 cut(s) 353
AspS9I GGNCC 2 cut(s) 88, 152
AsuHPI GGTGA 3 cut(s) 91, 230, 499
BalI TGGCCA 1 cut(s) 339
BauI CACGAG 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 5 cut(s) 21, 29, 32, 35, 460
BccI CCATC 1 cut(s) 65
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 3 cut(s) 86, 92, 341
BcoDI GTCTC 2 cut(s) 58, 269
BisI GCNGC 5 cut(s) 10, 18, 21, 24, 474
BlsI GCNGC 5 cut(s) 11, 19, 22, 25, 475
Bme1390I CCNGG 3 cut(s) 86, 92, 341
BmgT120I GGNCC 2 cut(s) 88, 152
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 3 cut(s) 86, 92, 341
BmsI GCATC 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 2 cut(s) 161, 521
BsaJI CCNNGG 2 cut(s) 69, 85
BsaWI WCCGGW 1 cut(s) 445
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 136, 150, 483
Bse1I ACTGG 3 cut(s) 39, 340, 380
Bse3DI GCAATG 1 cut(s) 19
BseAI TCCGGA 1 cut(s) 445
BseBI CCWGG 3 cut(s) 86, 92, 341
BseDI CCNNGG 2 cut(s) 69, 85
BseGI GGATG 3 cut(s) 10, 120, 126
BseLI CCNNNNNNNGG 4 cut(s) 91, 136, 150, 483
BseMI GCAATG 1 cut(s) 19
BseMII CTCAG 2 cut(s) 280, 359
BseNI ACTGG 3 cut(s) 39, 340, 380
BseRI GAGGAG 3 cut(s) 293, 526, 529
BseXI GCAGC 5 cut(s) 21, 29, 32, 35, 460
BseYI CCCAGC 1 cut(s) 387
BshFI GGCC 5 cut(s) 38, 90, 154, 182, 339
BsiSI CCGG 1 cut(s) 446
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 4 cut(s) 91, 136, 150, 483
BsmAI GTCTC 2 cut(s) 58, 269
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 5 cut(s) 38, 90, 154, 182, 339
Bsp13I TCCGGA 1 cut(s) 445
Bsp143I GATC 2 cut(s) 253, 481
BspACI CCGC 2 cut(s) 26, 109
BspANI GGCC 5 cut(s) 38, 90, 154, 182, 339
BspCNI CTCAG 2 cut(s) 279, 358
BspEI TCCGGA 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 19
BsrI ACTGG 3 cut(s) 39, 340, 380
BssECI CCNNGG 2 cut(s) 69, 85
BssMI GATC 2 cut(s) 253, 481
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 3 cut(s) 86, 92, 341
Bst4CI ACNGT 3 cut(s) 245, 311, 400
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 3 cut(s) 266, 345, 369
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 3 cut(s) 10, 120, 126
BstKTI GATC 2 cut(s) 256, 484
BstMAI GTCTC 2 cut(s) 58, 269
BstMBI GATC 2 cut(s) 253, 481
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 3 cut(s) 86, 92, 341
BstSCI CCNGG 3 cut(s) 84, 90, 339
BstV1I GCAGC 5 cut(s) 21, 29, 32, 35, 460
BstV2I GAAGAC 1 cut(s) 228
BstXI CCANNNNNNTGG 1 cut(s) 387
BsuRI GGCC 5 cut(s) 38, 90, 154, 182, 339
BtsCI GGATG 3 cut(s) 10, 120, 126
BtsIMutI CAGTG 2 cut(s) 333, 463
Cac8I GCNNGC 1 cut(s) 297
Cfr13I GGNCC 2 cut(s) 88, 152
CseI GACGC 1 cut(s) 382
Csp6I GTAC 1 cut(s) 383
CviQI GTAC 1 cut(s) 383
DdeI CTNAG 3 cut(s) 266, 345, 369
DpnI GATC 2 cut(s) 255, 483
DpnII GATC 2 cut(s) 253, 481
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 2 cut(s) 36, 337
Eco130I CCWWGG 1 cut(s) 69
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoRII CCWGG 3 cut(s) 84, 90, 339
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 58
FaiI YATR 2 cut(s) 404, 439
FalI AAGNNNNNCTT 6 cut(s) 53, 85, 170, 202, 213, 245
FaqI GGGAC 1 cut(s) 404
Fnu4HI GCNGC 5 cut(s) 10, 18, 21, 24, 474
FokI GGATG 3 cut(s) 17, 107, 113
Fsp4HI GCNGC 5 cut(s) 10, 18, 21, 24, 474
GluI GCNGC 5 cut(s) 10, 18, 21, 24, 474
GsaI CCCAGC 1 cut(s) 391
HaeIII GGCC 5 cut(s) 38, 90, 154, 182, 339
HapII CCGG 1 cut(s) 446
HgaI GACGC 1 cut(s) 382
HinfI GANTC 2 cut(s) 281, 449
HpaII CCGG 1 cut(s) 446
HphI GGTGA 3 cut(s) 91, 230, 499
Hpy188I TCNGA 2 cut(s) 269, 348
Hpy188III TCNNGA 3 cut(s) 61, 134, 446
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 3 cut(s) 245, 311, 400
HpyCH4V TGCA 2 cut(s) 17, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 3 cut(s) 266, 345, 369
Kpn2I TCCGGA 1 cut(s) 445
Kzo9I GATC 2 cut(s) 253, 481
Lsp1109I GCAGC 5 cut(s) 21, 29, 32, 35, 460
LweI GCATC 1 cut(s) 349
MaeIII GTNAC 2 cut(s) 245, 331
MalI GATC 2 cut(s) 255, 483
MboI GATC 2 cut(s) 253, 481
MboII GAAGA 3 cut(s) 233, 434, 502
MlsI TGGCCA 1 cut(s) 339
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 2 cut(s) 290, 458
MmeI TCCRAC 2 cut(s) 141, 486
Mox20I TGGCCA 1 cut(s) 339
MroI TCCGGA 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 353
MscI TGGCCA 1 cut(s) 339
MseI TTAA 2 cut(s) 237, 258
MslI CAYNNNNRTG 1 cut(s) 407
Msp20I TGGCCA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 1 cut(s) 446
MspR9I CCNGG 3 cut(s) 86, 92, 341
MvaI CCWGG 3 cut(s) 86, 92, 341
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 2 cut(s) 253, 481
NlaIV GGNNCC 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 353
PkrI GCNGC 5 cut(s) 11, 19, 22, 25, 475
PleI GAGTC 2 cut(s) 289, 457
PpsI GAGTC 2 cut(s) 289, 457
Psp6I CCWGG 3 cut(s) 84, 90, 339
PspFI CCCAGC 1 cut(s) 387
PspGI CCWGG 3 cut(s) 84, 90, 339
PspN4I GGNNCC 1 cut(s) 142
PspPI GGNCC 2 cut(s) 88, 152
RsaI GTAC 1 cut(s) 384
RsaNI GTAC 1 cut(s) 383
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 2 cut(s) 237, 258
SatI GCNGC 5 cut(s) 10, 18, 21, 24, 474
Sau3AI GATC 2 cut(s) 253, 481
Sau96I GGNCC 2 cut(s) 88, 152
SchI GAGTC 2 cut(s) 290, 458
ScrFI CCNGG 3 cut(s) 86, 92, 341
SetI ASST 9 cut(s) 71, 96, 104, 206, 220, 252, 289, 423, 521
SfaNI GCATC 1 cut(s) 349
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 176, 500
SmoI CTYRAG 2 cut(s) 176, 500
SsiI CCGC 2 cut(s) 26, 109
StyD4I CCNGG 3 cut(s) 84, 90, 339
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 3 cut(s) 245, 311, 400
TaqI TCGA 1 cut(s) 357
Tru1I TTAA 2 cut(s) 237, 258
Tru9I TTAA 2 cut(s) 237, 258
TscAI CASTG 2 cut(s) 340, 463
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 5 cut(s) 9, 17, 20, 23, 473
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 1 cut(s) 428
TspRI CASTG 2 cut(s) 340, 463
XagI CCTNNNNNAGG 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.