Rmu_sc0006184.1_g000013

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006184.1
OG0001597
Physical Location & Seq
Forward (+)
77479 .. 83735
6257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006184.1_g000013.1.cds

Sequence Viewer

Length: 813 bp
atggatggcagcaatgcagcagcagcggaggaattggccgtgggttgctttgtctccatcaagaccaccttgggcgacgacttccagggccaggtcatcaccttcgaccgcccttccaacatcctcatccttcaagagggtttgaaaggagggcctaagcggaatatcaggctactcaaggccaactatatcaaggagttgagctatttgggtcaagctgaagacccacttgacattaagaactgttaccttgatcttaatagtctcagagccagagatactcaggtagtttacttgctacccaatttggaacaagcagaggcagagtgtgagaggatcggagttggtgtcacaagccaggctcagagcattttcgatgccttgtctaagacgcgagtttttctagcttttggcagtaatgaacggttgaatagccgtgacataatgaagagtcgcggcagagagaaccccgcggccgagatcgatccttcctcggcccacaatcaatcgatcagaagtcagatcagcgcaagatctacaatctataacaaaacctcgcttgccctaccggccgcaagttggcgcgcccgcgcacacgtcaccactccagaaggacacgtatttgttgaggtggtgactggtgcacaccaaaatctctcttccatcgggcaaaagttggattttcttgacccaatcgaagaatatgggcaatggcttgacatatttcttgacccagtcgaaagaggctgccagctcagcccaagaagtaacatggctgacgtcagcggaagcaaggagagggagacgcgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

270

Amino Acids

29.77

Weight (kDa)

5.51

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 783
AccII CGCG 6 cut(s) 394, 456, 473, 585, 591, 808
AciI CCGC 9 cut(s) 26, 109, 160, 456, 471, 473, 573, 589, 786
AclWI GGATC 2 cut(s) 344, 479
AcoI YGGCCR 3 cut(s) 36, 474, 570
AcuI CTGAAG 1 cut(s) 240
AcyI GRCGYC 1 cut(s) 780
AfiI CCNNNNNNNGG 3 cut(s) 91, 136, 579
AflIII ACRYGT 3 cut(s) 595, 616, 806
AgsI TTSAA 3 cut(s) 134, 145, 430
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 3 cut(s) 84, 90, 357
AluBI AGCT 4 cut(s) 204, 218, 407, 754
AluI AGCT 4 cut(s) 204, 218, 407, 754
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 3 cut(s) 58, 269, 797
Alw44I GTGCAC 1 cut(s) 642
AlwI GGATC 2 cut(s) 344, 479
AoxI GGCC 7 cut(s) 36, 88, 152, 180, 474, 495, 570
ApaLI GTGCAC 1 cut(s) 642
ApeKI GCWGC 5 cut(s) 9, 17, 20, 23, 747
AscI GGCGCGCC 1 cut(s) 583
AspLEI GCGC 4 cut(s) 530, 585, 587, 593
AspS9I GGNCC 3 cut(s) 88, 152, 496
AsuHPI GGTGA 3 cut(s) 91, 592, 646
BaeGI GKGCMC 1 cut(s) 646
BaeI ACNNNNGTAYC 2 cut(s) 270, 303
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 5 cut(s) 21, 29, 32, 35, 734
BccI CCATC 2 cut(s) 65, 671
BceAI ACGGC 2 cut(s) 23, 420
BciT130I CCWGG 3 cut(s) 86, 92, 359
BcoDI GTCTC 3 cut(s) 58, 269, 797
BfaI CTAG 1 cut(s) 404
BglI GCCNNNNNGGC 1 cut(s) 569
BglII AGATCT 1 cut(s) 533
BisI GCNGC 8 cut(s) 10, 18, 21, 24, 457, 474, 573, 748
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 8 cut(s) 11, 19, 22, 25, 458, 475, 574, 749
Bme1390I CCNGG 3 cut(s) 86, 92, 359
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 3 cut(s) 88, 152, 496
BmrFI CCNGG 3 cut(s) 86, 92, 359
BmrI ACTGGG 1 cut(s) 728
BmsI GCATC 1 cut(s) 367
BmuI ACTGGG 1 cut(s) 728
BpiI GAAGAC 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 1 cut(s) 156
Bpu1102I GCTNAGC 1 cut(s) 755
BpuEI CTTGAG 1 cut(s) 161
Bsa29I ATCGAT 2 cut(s) 483, 509
BsaAI YACGTR 1 cut(s) 619
BsaHI GRCGYC 1 cut(s) 780
BsaJI CCNNGG 5 cut(s) 39, 69, 85, 471, 492
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 136, 579
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 2 cut(s) 643, 734
Bse3DI GCAATG 2 cut(s) 19, 716
BseBI CCWGG 3 cut(s) 86, 92, 359
BseCI ATCGAT 2 cut(s) 483, 509
BseDI CCNNGG 5 cut(s) 39, 69, 85, 471, 492
BseGI GGATG 3 cut(s) 10, 120, 126
BseLI CCNNNNNNNGG 3 cut(s) 91, 136, 579
BseMI GCAATG 2 cut(s) 19, 716
BseMII CTCAG 4 cut(s) 280, 296, 377, 769
BseNI ACTGG 2 cut(s) 643, 734
BsePI GCGCGC 1 cut(s) 583
BseSI GKGCMC 1 cut(s) 646
BseX3I CGGCCG 2 cut(s) 474, 570
BseXI GCAGC 5 cut(s) 21, 29, 32, 35, 734
Bsh1236I CGCG 6 cut(s) 394, 456, 473, 585, 591, 808
Bsh1285I CGRYCG 3 cut(s) 109, 477, 573
BshFI GGCC 7 cut(s) 38, 90, 154, 182, 476, 497, 572
BshVI ATCGAT 2 cut(s) 483, 509
BsiEI CGRYCG 3 cut(s) 109, 477, 573
BsiHKAI GWGCWC 1 cut(s) 646
BsiSI CCGG 1 cut(s) 569
BslI CCNNNNNNNGG 3 cut(s) 91, 136, 579
BsmAI GTCTC 3 cut(s) 58, 269, 797
BsmBI CGTCTC 1 cut(s) 797
BsnI GGCC 7 cut(s) 38, 90, 154, 182, 476, 497, 572
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 7 cut(s) 253, 336, 480, 484, 510, 522, 533
Bsp1720I GCTNAGC 1 cut(s) 755
BspACI CCGC 9 cut(s) 26, 109, 160, 456, 471, 473, 573, 589, 786
BspANI GGCC 7 cut(s) 38, 90, 154, 182, 476, 497, 572
BspCNI CTCAG 4 cut(s) 279, 295, 376, 768
BspDI ATCGAT 2 cut(s) 483, 509
BspFNI CGCG 6 cut(s) 394, 456, 473, 585, 591, 808
BspPI GGATC 2 cut(s) 344, 479
BsrDI GCAATG 2 cut(s) 19, 716
BsrFI RCCGGY 1 cut(s) 568
BsrI ACTGG 2 cut(s) 643, 734
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 5 cut(s) 39, 69, 85, 471, 492
BssHII GCGCGC 1 cut(s) 583
BssMI GATC 7 cut(s) 253, 336, 480, 484, 510, 522, 533
BssNI GRCGYC 1 cut(s) 780
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 3 cut(s) 86, 92, 359
Bst4CI ACNGT 2 cut(s) 245, 426
Bst6I CTCTTC 2 cut(s) 443, 664
BstACI GRCGYC 1 cut(s) 780
BstBAI YACGTR 1 cut(s) 619
BstC8I GCNNGC 4 cut(s) 561, 585, 589, 752
BstDEI CTNAG 6 cut(s) 156, 266, 282, 363, 387, 755
BstDSI CCRYGG 2 cut(s) 39, 471
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 3 cut(s) 10, 120, 126
BstFNI CGCG 6 cut(s) 394, 456, 473, 585, 591, 808
BstHHI GCGC 4 cut(s) 530, 585, 587, 593
BstKTI GATC 7 cut(s) 256, 339, 483, 487, 513, 525, 536
BstMAI GTCTC 3 cut(s) 58, 269, 797
BstMBI GATC 7 cut(s) 253, 336, 480, 484, 510, 522, 533
BstMCI CGRYCG 3 cut(s) 109, 477, 573
BstMWI GCNNNNNNNGC 3 cut(s) 23, 569, 756
BstNI CCWGG 3 cut(s) 86, 92, 359
BstSCI CCNGG 3 cut(s) 84, 90, 357
BstSLI GKGCMC 1 cut(s) 646
BstUI CGCG 6 cut(s) 394, 456, 473, 585, 591, 808
BstV1I GCAGC 5 cut(s) 21, 29, 32, 35, 734
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 1 cut(s) 533
BstYI RGATCY 1 cut(s) 533
BstZI CGGCCG 2 cut(s) 474, 570
Bsu15I ATCGAT 2 cut(s) 483, 509
BsuRI GGCC 7 cut(s) 38, 90, 154, 182, 476, 497, 572
BsuTUI ATCGAT 2 cut(s) 483, 509
BtgI CCRYGG 2 cut(s) 39, 471
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 3 cut(s) 10, 120, 126
Cac8I GCNNGC 4 cut(s) 561, 585, 589, 752
CfoI GCGC 4 cut(s) 530, 585, 587, 593
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 3 cut(s) 88, 152, 496
Cfr42I CCGCGG 1 cut(s) 474
ClaI ATCGAT 2 cut(s) 483, 509
CseI GACGC 1 cut(s) 400
CviAII CATG 1 cut(s) 772
DdeI CTNAG 6 cut(s) 156, 266, 282, 363, 387, 755
DpnI GATC 7 cut(s) 255, 338, 482, 486, 512, 524, 535
DpnII GATC 7 cut(s) 253, 336, 480, 484, 510, 522, 533
EaeI YGGCCR 3 cut(s) 36, 474, 570
EagI CGGCCG 2 cut(s) 474, 570
Eam1104I CTCTTC 2 cut(s) 443, 664
EarI CTCTTC 2 cut(s) 443, 664
EclXI CGGCCG 2 cut(s) 474, 570
Eco130I CCWWGG 1 cut(s) 69
Eco52I CGGCCG 2 cut(s) 474, 570
Eco57I CTGAAG 1 cut(s) 240
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 152
EcoRII CCWGG 3 cut(s) 84, 90, 357
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 797
FaeI CATG 1 cut(s) 775
FaiI YATR 6 cut(s) 189, 443, 546, 705, 722, 773
FalI AAGNNNNNCTT 4 cut(s) 53, 85, 213, 245
FatI CATG 1 cut(s) 771
FauI CCCGC 2 cut(s) 478, 596
Fnu4HI GCNGC 8 cut(s) 10, 18, 21, 24, 457, 474, 573, 748
FokI GGATG 3 cut(s) 17, 107, 113
Fsp4HI GCNGC 8 cut(s) 10, 18, 21, 24, 457, 474, 573, 748
FspBI CTAG 1 cut(s) 404
GlaI GCGC 4 cut(s) 529, 584, 586, 592
GluI GCNGC 8 cut(s) 10, 18, 21, 24, 457, 474, 573, 748
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 7 cut(s) 38, 90, 154, 182, 476, 497, 572
HapII CCGG 1 cut(s) 569
HgaI GACGC 1 cut(s) 400
HhaI GCGC 4 cut(s) 530, 585, 587, 593
Hin1I GRCGYC 1 cut(s) 780
Hin1II CATG 1 cut(s) 775
Hin6I GCGC 4 cut(s) 528, 583, 585, 591
HinP1I GCGC 4 cut(s) 528, 583, 585, 591
HinfI GANTC 1 cut(s) 451
HpaII CCGG 1 cut(s) 569
HphI GGTGA 3 cut(s) 91, 592, 646
Hpy166II GTNNAC 2 cut(s) 292, 644
Hpy188I TCNGA 5 cut(s) 269, 341, 366, 515, 522
Hpy188III TCNNGA 5 cut(s) 61, 134, 608, 686, 728
Hpy8I GTNNAC 2 cut(s) 292, 644
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 5 cut(s) 112, 123, 140, 498, 605
HpyCH4III ACNGT 2 cut(s) 245, 426
HpyCH4IV ACGT 3 cut(s) 597, 618, 780
HpyCH4V TGCA 2 cut(s) 17, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 569, 756
HpyF3I CTNAG 6 cut(s) 156, 266, 282, 363, 387, 755
HpySE526I ACGT 3 cut(s) 597, 618, 780
Hsp92I GRCGYC 1 cut(s) 780
Hsp92II CATG 1 cut(s) 775
HspAI GCGC 4 cut(s) 528, 583, 585, 591
KspI CCGCGG 1 cut(s) 474
Kzo9I GATC 7 cut(s) 253, 336, 480, 484, 510, 522, 533
Lsp1109I GCAGC 5 cut(s) 21, 29, 32, 35, 734
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 404
MaeII ACGT 3 cut(s) 597, 618, 780
MaeIII GTNAC 6 cut(s) 245, 349, 437, 598, 634, 767
MalI GATC 7 cut(s) 255, 338, 482, 486, 512, 524, 535
MboI GATC 7 cut(s) 253, 336, 480, 484, 510, 522, 533
MboII GAAGA 4 cut(s) 233, 460, 651, 710
MflI RGATCY 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 2 cut(s) 32, 304
MluI ACGCGT 1 cut(s) 806
MlyI GAGTC 1 cut(s) 460
MmeI TCCRAC 2 cut(s) 141, 657
MseI TTAA 2 cut(s) 237, 258
MspA1I CMGCKG 3 cut(s) 26, 473, 786
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 3 cut(s) 86, 92, 359
MvaI CCWGG 3 cut(s) 86, 92, 359
MvnI CGCG 6 cut(s) 394, 456, 473, 585, 591, 808
MwoI GCNNNNNNNGC 3 cut(s) 23, 569, 756
NdeII GATC 7 cut(s) 253, 336, 480, 484, 510, 522, 533
NlaIII CATG 1 cut(s) 775
NmeAIII GCCGAG 2 cut(s) 473, 502
NmuCI GTSAC 4 cut(s) 349, 437, 598, 634
PalAI GGCGCGCC 1 cut(s) 583
PauI GCGCGC 1 cut(s) 583
PflFI GACNNNGTC 1 cut(s) 734
PkrI GCNGC 8 cut(s) 11, 19, 22, 25, 458, 475, 574, 749
PleI GAGTC 1 cut(s) 459
PpsI GAGTC 1 cut(s) 459
Ppu21I YACGTR 1 cut(s) 619
Psp6I CCWGG 3 cut(s) 84, 90, 357
PspGI CCWGG 3 cut(s) 84, 90, 357
PspPI GGNCC 3 cut(s) 88, 152, 496
PsuI RGATCY 1 cut(s) 533
PsyI GACNNNGTC 1 cut(s) 734
PteI GCGCGC 1 cut(s) 583
SacII CCGCGG 1 cut(s) 474
SaqAI TTAA 2 cut(s) 237, 258
SatI GCNGC 8 cut(s) 10, 18, 21, 24, 457, 474, 573, 748
Sau3AI GATC 7 cut(s) 253, 336, 480, 484, 510, 522, 533
Sau96I GGNCC 3 cut(s) 88, 152, 496
SchI GAGTC 1 cut(s) 460
ScrFI CCNGG 3 cut(s) 86, 92, 359
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 1 cut(s) 367
Sfr303I CCGCGG 1 cut(s) 474
SgrBI CCGCGG 1 cut(s) 474
SgsI GGCGCGCC 1 cut(s) 583
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 2 cut(s) 32, 304
SsiI CCGC 9 cut(s) 26, 109, 160, 456, 471, 473, 573, 589, 786
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 3 cut(s) 84, 90, 357
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 245, 426
TaiI ACGT 3 cut(s) 600, 621, 783
TaqI TCGA 6 cut(s) 105, 375, 483, 509, 696, 738
TasI AATT 2 cut(s) 32, 304
TauI GCSGC 3 cut(s) 459, 476, 575
Tru1I TTAA 2 cut(s) 237, 258
Tru9I TTAA 2 cut(s) 237, 258
TseFI GTSAC 4 cut(s) 349, 437, 598, 634
TseI GCWGC 5 cut(s) 9, 17, 20, 23, 747
Tsp45I GTSAC 4 cut(s) 349, 437, 598, 634
TspDTI ATGAA 2 cut(s) 435, 461
Tth111I GACNNNGTC 1 cut(s) 734
VneI GTGCAC 1 cut(s) 642
XagI CCTNNNNNAGG 1 cut(s) 134
XspI CTAG 1 cut(s) 404
ZraI GACGTC 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.