MD13G1087800.v1.1

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001597
Physical Location & Seq
Reverse (-)
6149919 .. 6151560
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1087800.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGCCATGGATGGCAGCGTCACGGAGAAGTTGGCCATCGGGTGCTTGGTCTCCGTCAAGACCACGCTAAGCGACGACTTCCAAGGCCAAGTCATCACCTTCGATCGCCCCTCCAACATCGTCGTCATTCATATCCTTTCACCGTTCGCAATTTATCAATTCAAGGGTTCGAAAGGCGGCGCGCGGCGGAACATACGGCTGCTGAAGGCGAACTATATAAAGGAGCTGTCGTACTTGGGTCAGGCGGAAGACCCACTTGATATCAAAAAGTGTTTCCTTGATCTCAATAGTCTGCGAGCCAGGGAGGAATTGGCGATTAGACAAGCGGAGGCAGAGTCGGAGAGGATCGGCGTTGGGGTCACTGGCCAGGCGCAGAACATTTTCGATGCCTTGTCTAAGACGCTTCCAGTGCGCTGGGACAAGACTGTCATAGTTGTGATGAATGAGGTCCGTGTAAGCAGTCCATATACTCCCGAGTCTGTCAGTGGAGGAACTCCTGCTGCCAATGAGCGGGTGAAGAAAGTGCTTGAGCTGGAGAGGAGGAGGTTGCAAACTCGTGGTGGTGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

191

Amino Acids

20.98

Weight (kDa)

9.3

Isoelectric Point (pI)

52.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM12_LSM PF21166 10 - 75 1.9e-08 LSM12, LSM domain
AD PF09793 93 - 175 3.5e-26 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AccBSI CCGCTC 1 cut(s) 511
AccII CGCG 2 cut(s) 182, 184
AciI CCGC 6 cut(s) 177, 184, 187, 245, 326, 511
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 3 cut(s) 3, 33, 364
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 510
AgsI TTSAA 1 cut(s) 163
AjnI CCWGG 2 cut(s) 299, 366
AluBI AGCT 2 cut(s) 226, 532
AluI AGCT 2 cut(s) 226, 532
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 1 cut(s) 353
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 4 cut(s) 3, 33, 85, 364
ApeKI GCWGC 3 cut(s) 15, 199, 500
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 4 cut(s) 182, 184, 373, 414
AspS9I GGNCC 1 cut(s) 448
AsuHPI GGTGA 3 cut(s) 88, 132, 526
AsuII TTCGAA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 1 cut(s) 448
BalI TGGCCA 3 cut(s) 5, 35, 366
BauI CACGAG 1 cut(s) 555
BbsI GAAGAC 1 cut(s) 255
BbvI GCAGC 3 cut(s) 27, 186, 487
BccI CCATC 2 cut(s) 5, 44
BceAI ACGGC 1 cut(s) 212
BciT130I CCWGG 2 cut(s) 301, 368
BcoDI GTCTC 1 cut(s) 55
BisI GCNGC 5 cut(s) 16, 178, 185, 200, 501
BlpI GCTNAGC 1 cut(s) 68
BlsI GCNGC 5 cut(s) 17, 179, 186, 201, 502
Bme1390I CCNGG 2 cut(s) 301, 368
Bme18I GGWCC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 448
BmrFI CCNGG 2 cut(s) 301, 368
BmsI GCATC 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 255
BpmI CTGGAG 1 cut(s) 554
Bpu1102I GCTNAGC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 548
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 3 cut(s) 6, 82, 300
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse1I ACTGG 2 cut(s) 367, 407
BseBI CCWGG 2 cut(s) 301, 368
BseDI CCNNGG 3 cut(s) 6, 82, 300
BseGI GGATG 1 cut(s) 16
BseLI CCNNNNNNNGG 1 cut(s) 510
BseNI ACTGG 2 cut(s) 367, 407
BsePI GCGCGC 1 cut(s) 180
BseRI GAGGAG 2 cut(s) 553, 556
BseXI GCAGC 3 cut(s) 27, 186, 487
BseYI CCCAGC 1 cut(s) 414
Bsh1236I CGCG 2 cut(s) 182, 184
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 5, 35, 87, 366
BsiEI CGRYCG 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 473
BslFI GGGAC 1 cut(s) 431
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 431
BsnI GGCC 4 cut(s) 5, 35, 87, 366
Bso31I GGTCTC 1 cut(s) 55
BsoBI CYCGRG 1 cut(s) 473
Bsp119I TTCGAA 1 cut(s) 170
Bsp143I GATC 3 cut(s) 103, 280, 345
Bsp1720I GCTNAGC 1 cut(s) 68
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 6 cut(s) 177, 184, 187, 245, 326, 511
BspANI GGCC 4 cut(s) 5, 35, 87, 366
BspFNI CGCG 2 cut(s) 182, 184
BspPI GGATC 1 cut(s) 353
BspT104I TTCGAA 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 55
BsrBI CCGCTC 1 cut(s) 511
BsrI ACTGG 2 cut(s) 367, 407
BssECI CCNNGG 3 cut(s) 6, 82, 300
BssHII GCGCGC 1 cut(s) 180
BssMI GATC 3 cut(s) 103, 280, 345
BssSI CACGAG 1 cut(s) 555
BssT1I CCWWGG 2 cut(s) 6, 82
Bst2BI CACGAG 1 cut(s) 555
Bst2UI CCWGG 2 cut(s) 301, 368
Bst4CI ACNGT 2 cut(s) 144, 427
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 182, 297
BstDEI CTNAG 2 cut(s) 68, 396
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 16
BstFNI CGCG 2 cut(s) 182, 184
BstHHI GCGC 4 cut(s) 182, 184, 373, 414
BstKTI GATC 3 cut(s) 106, 283, 348
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 3 cut(s) 103, 280, 345
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 409
BstNI CCWGG 2 cut(s) 301, 368
BstSCI CCNGG 2 cut(s) 299, 366
BstUI CGCG 2 cut(s) 182, 184
BstV1I GCAGC 3 cut(s) 27, 186, 487
BstV2I GAAGAC 1 cut(s) 255
BstXI CCANNNNNNTGG 1 cut(s) 414
BsuRI GGCC 4 cut(s) 5, 35, 87, 366
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 1 cut(s) 16
BtsIMutI CAGTG 3 cut(s) 360, 414, 490
Cac8I GCNNGC 2 cut(s) 182, 297
CfoI GCGC 4 cut(s) 182, 184, 373, 414
Cfr13I GGNCC 1 cut(s) 448
CseI GACGC 2 cut(s) 7, 409
Csp6I GTAC 1 cut(s) 232
CviAII CATG 1 cut(s) 7
CviJI RGCY 8 cut(s) 5, 35, 87, 199, 226, 299, 366, 532
CviKI_1 RGCY 8 cut(s) 5, 35, 87, 199, 226, 299, 366, 532
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 2 cut(s) 68, 396
DpnI GATC 3 cut(s) 105, 282, 347
DpnII GATC 3 cut(s) 103, 280, 345
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 3 cut(s) 3, 33, 364
EciI GGCGGA 2 cut(s) 202, 260
Eco130I CCWWGG 2 cut(s) 6, 82
Eco31I GGTCTC 1 cut(s) 55
Eco32I GATATC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 299, 366
EcoRV GATATC 1 cut(s) 262
EcoT14I CCWWGG 2 cut(s) 6, 82
ErhI CCWWGG 2 cut(s) 6, 82
FaeI CATG 1 cut(s) 10
FaiI YATR 8 cut(s) 8, 132, 194, 216, 218, 431, 466, 468
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 1 cut(s) 431
FatI CATG 1 cut(s) 6
FauI CCCGC 1 cut(s) 504
Fnu4HI GCNGC 5 cut(s) 16, 178, 185, 200, 501
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 5 cut(s) 16, 178, 185, 200, 501
GlaI GCGC 4 cut(s) 181, 183, 372, 413
GluI GCNGC 5 cut(s) 16, 178, 185, 200, 501
GsaI CCCAGC 1 cut(s) 418
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 4 cut(s) 5, 35, 87, 366
HgaI GACGC 2 cut(s) 7, 409
HhaI GCGC 4 cut(s) 182, 184, 373, 414
Hin1II CATG 1 cut(s) 10
Hin6I GCGC 4 cut(s) 180, 182, 371, 412
HinP1I GCGC 4 cut(s) 180, 182, 371, 412
HinfI GANTC 2 cut(s) 335, 476
HphI GGTGA 3 cut(s) 88, 132, 526
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 2 cut(s) 58, 473
Hpy99I CGWCG 2 cut(s) 77, 125
HpyAV CCTTC 2 cut(s) 109, 199
HpyCH4III ACNGT 2 cut(s) 144, 427
HpyCH4V TGCA 1 cut(s) 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 409
HpyF3I CTNAG 2 cut(s) 68, 396
Hsp92II CATG 1 cut(s) 10
HspAI GCGC 4 cut(s) 180, 182, 371, 412
Kzo9I GATC 3 cut(s) 103, 280, 345
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 3 cut(s) 27, 186, 487
LweI GCATC 1 cut(s) 376
MaeIII GTNAC 2 cut(s) 19, 358
MalI GATC 3 cut(s) 105, 282, 347
MbiI CCGCTC 1 cut(s) 511
MboI GATC 3 cut(s) 103, 280, 345
MboII GAAGA 2 cut(s) 260, 529
MlsI TGGCCA 3 cut(s) 5, 35, 366
MluCI AATT 3 cut(s) 150, 158, 308
MluNI TGGCCA 3 cut(s) 5, 35, 366
MlyI GAGTC 2 cut(s) 344, 485
MmeI TCCRAC 2 cut(s) 138, 318
MnlI CCTC 9 cut(s) 121, 298, 322, 336, 439, 482, 531, 534, 537
Mox20I TGGCCA 3 cut(s) 5, 35, 366
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 3 cut(s) 5, 35, 366
MslI CAYNNNNRTG 1 cut(s) 434
Msp20I TGGCCA 3 cut(s) 5, 35, 366
MspR9I CCNGG 2 cut(s) 301, 368
MvaI CCWGG 2 cut(s) 301, 368
MvnI CGCG 2 cut(s) 182, 184
MwoI GCNNNNNNNGC 1 cut(s) 409
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 103, 280, 345
NlaIII CATG 1 cut(s) 10
NmuCI GTSAC 2 cut(s) 19, 358
NspV TTCGAA 1 cut(s) 170
PauI GCGCGC 1 cut(s) 180
PdmI GAANNNNTTC 1 cut(s) 380
PkrI GCNGC 5 cut(s) 17, 179, 186, 201, 502
Ple19I CGATCG 1 cut(s) 106
PleI GAGTC 2 cut(s) 343, 484
PpsI GAGTC 2 cut(s) 343, 484
Psp6I CCWGG 2 cut(s) 299, 366
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 2 cut(s) 299, 366
PspPI GGNCC 1 cut(s) 448
PteI GCGCGC 1 cut(s) 180
PvuI CGATCG 1 cut(s) 106
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 434
SatI GCNGC 5 cut(s) 16, 178, 185, 200, 501
Sau3AI GATC 3 cut(s) 103, 280, 345
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 2 cut(s) 344, 485
ScrFI CCNGG 2 cut(s) 301, 368
SetI ASST 5 cut(s) 101, 228, 450, 534, 548
SfaNI GCATC 1 cut(s) 376
SfuI TTCGAA 1 cut(s) 170
SinI GGWCC 1 cut(s) 448
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 3 cut(s) 150, 158, 308
SsiI CCGC 6 cut(s) 177, 184, 187, 245, 326, 511
StyD4I CCNGG 2 cut(s) 299, 366
StyI CCWWGG 2 cut(s) 6, 82
TaaI ACNGT 2 cut(s) 144, 427
TaqI TCGA 3 cut(s) 102, 170, 384
TasI AATT 3 cut(s) 150, 158, 308
TauI GCSGC 2 cut(s) 180, 187
TscAI CASTG 3 cut(s) 367, 414, 490
TseFI GTSAC 2 cut(s) 19, 358
TseI GCWGC 3 cut(s) 15, 199, 500
Tsp45I GTSAC 2 cut(s) 19, 358
TspDTI ATGAA 2 cut(s) 119, 455
TspGWI ACGGA 3 cut(s) 38, 43, 440
TspRI CASTG 3 cut(s) 367, 414, 490
VpaK11BI GGWCC 1 cut(s) 448
XcmI CCANNNNNNNNNTGG 2 cut(s) 43, 307
XmnI GAANNNNTTC 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.