Rh4CG438500

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001597
Physical Location & Seq
Forward (+)
67624168 .. 67626730
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG438500.1

Sequence Viewer

Length: 384 bp
ATGGCCATGGATGGCAGCAATGCAGCAGCAGCGGAGGAATTGGCCGTGGGTTGCTTCGTCTCCATCAAGACCACCTTGGGCGACGACTTCCAGGGCCAGGTCATCACCTTCGACCGCCCCTCCAACATCCTCATCCTTCAAGAGGGTTTGAAAGGAGGGCCTAAGCGGAACATCAGGCTACTCAAGGCCAACTATATCAAGGAGTTGAGCTATATGGGTCAAGCTGAAGACCCACTTGACATTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCCGAGAGGAATCAGCTATCAGACAAGCAGAGGCAGAGTGTGAGAGGATCGGAGTTGGTGTCACCAGCCAGGCTCAGAACATTTTCGATGCCTTGTCTAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

127

Amino Acids

13.85

Weight (kDa)

4.85

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM12_LSM PF21166 12 - 68 1e-13 LSM12, LSM domain
AD PF09793 86 - 127 1.2e-09 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 32, 115, 166
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 2 cut(s) 3, 42
AcuI CTGAAG 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 97, 142
AgsI TTSAA 2 cut(s) 140, 151
AjiI CACGTC 1 cut(s) 381
AjnI CCWGG 3 cut(s) 90, 96, 345
AluBI AGCT 3 cut(s) 210, 224, 293
AluI AGCT 3 cut(s) 210, 224, 293
Alw26I GTCTC 2 cut(s) 64, 275
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 5 cut(s) 3, 42, 94, 158, 186
ApeKI GCWGC 4 cut(s) 15, 23, 26, 29
Asp700I GAANNNNTTC 1 cut(s) 359
AspS9I GGNCC 2 cut(s) 94, 158
AsuHPI GGTGA 2 cut(s) 97, 331
AvaI CYCGRG 1 cut(s) 279
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 4 cut(s) 27, 35, 38, 41
BccI CCATC 2 cut(s) 5, 71
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 92, 98, 347
BcoDI GTCTC 2 cut(s) 64, 275
BisI GCNGC 4 cut(s) 16, 24, 27, 30
BlsI GCNGC 4 cut(s) 17, 25, 28, 31
Bme1390I CCNGG 3 cut(s) 92, 98, 347
BmeT110I CYCGRG 1 cut(s) 279
BmgBI CACGTC 1 cut(s) 381
BmgT120I GGNCC 2 cut(s) 94, 158
BmrFI CCNGG 3 cut(s) 92, 98, 347
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 167
BsaJI CCNNGG 4 cut(s) 6, 45, 75, 91
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 321, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 142
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 3 cut(s) 92, 98, 347
BseDI CCNNGG 4 cut(s) 6, 45, 75, 91
BseGI GGATG 3 cut(s) 16, 126, 132
BseLI CCNNNNNNNGG 2 cut(s) 97, 142
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 2 cut(s) 286, 365
BseXI GCAGC 4 cut(s) 27, 35, 38, 41
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 5 cut(s) 5, 44, 96, 160, 188
BsiEI CGRYCG 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 279
BslI CCNNNNNNNGG 2 cut(s) 97, 142
BsmAI GTCTC 2 cut(s) 64, 275
BsmBI CGTCTC 1 cut(s) 64
BsnI GGCC 5 cut(s) 5, 44, 96, 160, 188
BsoBI CYCGRG 1 cut(s) 279
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 2 cut(s) 259, 324
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 3 cut(s) 32, 115, 166
BspANI GGCC 5 cut(s) 5, 44, 96, 160, 188
BspCNI CTCAG 2 cut(s) 285, 364
BspPI GGATC 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 25
BssECI CCNNGG 4 cut(s) 6, 45, 75, 91
BssMI GATC 2 cut(s) 259, 324
BssT1I CCWWGG 2 cut(s) 6, 75
Bst2UI CCWGG 3 cut(s) 92, 98, 347
Bst4CI ACNGT 1 cut(s) 251
BstDEI CTNAG 4 cut(s) 162, 272, 351, 375
BstDSI CCRYGG 2 cut(s) 6, 45
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 3 cut(s) 16, 126, 132
BstKTI GATC 2 cut(s) 262, 327
BstMAI GTCTC 2 cut(s) 64, 275
BstMBI GATC 2 cut(s) 259, 324
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 3 cut(s) 92, 98, 347
BstSCI CCNGG 3 cut(s) 90, 96, 345
BstV1I GCAGC 4 cut(s) 27, 35, 38, 41
BstV2I GAAGAC 1 cut(s) 234
BsuRI GGCC 5 cut(s) 5, 44, 96, 160, 188
BtgI CCRYGG 2 cut(s) 6, 45
BtrI CACGTC 1 cut(s) 381
BtsCI GGATG 3 cut(s) 16, 126, 132
Cfr13I GGNCC 2 cut(s) 94, 158
CviAII CATG 1 cut(s) 7
DdeI CTNAG 4 cut(s) 162, 272, 351, 375
DpnI GATC 2 cut(s) 261, 326
DpnII GATC 2 cut(s) 259, 324
EaeI YGGCCR 2 cut(s) 3, 42
Eco130I CCWWGG 2 cut(s) 6, 75
Eco24I GRGCYC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 279
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 3 cut(s) 90, 96, 345
EcoT14I CCWWGG 2 cut(s) 6, 75
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 2 cut(s) 6, 75
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 10
FaiI YATR 4 cut(s) 8, 195, 213, 215
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 219, 251
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 4 cut(s) 16, 24, 27, 30
FokI GGATG 3 cut(s) 23, 113, 119
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 4 cut(s) 16, 24, 27, 30
GluI GCNGC 4 cut(s) 16, 24, 27, 30
HaeIII GGCC 5 cut(s) 5, 44, 96, 160, 188
Hin1II CATG 1 cut(s) 10
HinfI GANTC 1 cut(s) 287
HphI GGTGA 2 cut(s) 97, 331
Hpy188I TCNGA 4 cut(s) 275, 299, 329, 354
Hpy188III TCNNGA 2 cut(s) 67, 140
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 118, 146
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 1 cut(s) 23
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 4 cut(s) 162, 272, 351, 375
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 1 cut(s) 10
Kzo9I GATC 2 cut(s) 259, 324
LpnPI CCDG 8 cut(s) 77, 83, 104, 110, 160, 332, 355, 359
Lsp1109I GCAGC 4 cut(s) 27, 35, 38, 41
LweI GCATC 1 cut(s) 355
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 2 cut(s) 251, 337
MalI GATC 2 cut(s) 261, 326
MboI GATC 2 cut(s) 259, 324
MboII GAAGA 1 cut(s) 239
MhlI GDGCHC 1 cut(s) 280
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 8 cut(s) 28, 130, 136, 140, 149, 277, 301, 315
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 359
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 243, 264
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 92, 98, 347
MvaI CCWGG 3 cut(s) 92, 98, 347
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 6
NdeII GATC 2 cut(s) 259, 324
NlaIII CATG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 359
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 4 cut(s) 17, 25, 28, 31
Psp6I CCWGG 3 cut(s) 90, 96, 345
PspGI CCWGG 3 cut(s) 90, 96, 345
PspPI GGNCC 2 cut(s) 94, 158
SaqAI TTAA 2 cut(s) 243, 264
SatI GCNGC 4 cut(s) 16, 24, 27, 30
Sau3AI GATC 2 cut(s) 259, 324
Sau96I GGNCC 2 cut(s) 94, 158
ScrFI CCNGG 3 cut(s) 92, 98, 347
SduI GDGCHC 1 cut(s) 280
SetI ASST 8 cut(s) 77, 102, 110, 212, 226, 258, 295, 383
SfaNI GCATC 1 cut(s) 355
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 1 cut(s) 38
SsiI CCGC 3 cut(s) 32, 115, 166
StyD4I CCNGG 3 cut(s) 90, 96, 345
StyI CCWWGG 2 cut(s) 6, 75
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 383
TaqI TCGA 2 cut(s) 111, 363
TasI AATT 1 cut(s) 38
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 2 cut(s) 243, 264
Tru9I TTAA 2 cut(s) 243, 264
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 4 cut(s) 15, 23, 26, 29
Tsp45I GTSAC 1 cut(s) 337
XagI CCTNNNNNAGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.