RchiOBHm_Chr7g0224821

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001597
Physical Location & Seq
Reverse (-)
47195380 .. 47196355
976 bp
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UTR
Exon/CDS
Intron
PRQ20131

Sequence Viewer

Length: 432 bp
ATGGCCATGGATGGCAGCAATGCAGCAGCAGCGGAGGAATTGGCCGTGGGTTGCTTCGTCTCCATCAAGACCACCTTGGGCGACGACTTCCAGGGCCAGGTCATCACCTTCGACCGCTCCTCCAACATCCTCATCCTTCAAGAGGGTTTGAAAGGAGGGCCTAAGCGGAACATCAGGCTACTCAAGGCCAACTATATCAAGGAGTTGAGCTATTTGGGTCAAGCTGAAGACCCACTTGACATTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCAGAGAGGAATCAGCTATCAGACAAGCAGAGGCAGAGTGTGAGAGGATCAGAGTTGGTGTCACCAGCCAGGCTCAAAACATTTTCGATGCCTTGTCTAAGACCTTAAGGGCTTCCAGTACGCTGGGACAAGACTGTCATAGTTGTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

143

Amino Acids

15.63

Weight (kDa)

4.96

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM12_LSM PF21166 12 - 68 8.9e-14 LSM12, LSM domain
AD PF09793 86 - 129 3.6e-10 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 411
AccBSI CCGCTC 1 cut(s) 117
AciI CCGC 3 cut(s) 32, 115, 166
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 2 cut(s) 3, 42
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 2 cut(s) 97, 142
AflII CTTAAG 1 cut(s) 382
AgsI TTSAA 2 cut(s) 140, 151
AjnI CCWGG 3 cut(s) 90, 96, 345
AluBI AGCT 3 cut(s) 210, 224, 293
AluI AGCT 3 cut(s) 210, 224, 293
Alw26I GTCTC 2 cut(s) 64, 275
AlwI GGATC 1 cut(s) 332
AoxI GGCC 5 cut(s) 3, 42, 94, 158, 186
ApeKI GCWGC 4 cut(s) 15, 23, 26, 29
AspS9I GGNCC 2 cut(s) 94, 158
AsuHPI GGTGA 2 cut(s) 97, 331
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 4 cut(s) 27, 35, 38, 41
BccI CCATC 2 cut(s) 5, 71
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 92, 98, 347
BcoDI GTCTC 2 cut(s) 64, 275
BfrI CTTAAG 1 cut(s) 382
BisI GCNGC 4 cut(s) 16, 24, 27, 30
BlsI GCNGC 4 cut(s) 17, 25, 28, 31
Bme1390I CCNGG 3 cut(s) 92, 98, 347
BmgT120I GGNCC 2 cut(s) 94, 158
BmrFI CCNGG 3 cut(s) 92, 98, 347
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 167
BsaJI CCNNGG 4 cut(s) 6, 45, 75, 91
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 142
Bse1I ACTGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 3 cut(s) 92, 98, 347
BseDI CCNNGG 4 cut(s) 6, 45, 75, 91
BseGI GGATG 3 cut(s) 16, 126, 132
BseLI CCNNNNNNNGG 2 cut(s) 97, 142
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 1 cut(s) 286
BseNI ACTGG 1 cut(s) 393
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 4 cut(s) 27, 35, 38, 41
BseYI CCCAGC 1 cut(s) 400
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 5 cut(s) 5, 44, 96, 160, 188
BsiEI CGRYCG 1 cut(s) 115
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 2 cut(s) 97, 142
BsmAI GTCTC 2 cut(s) 64, 275
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 5 cut(s) 5, 44, 96, 160, 188
Bsp143I GATC 2 cut(s) 259, 324
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 3 cut(s) 32, 115, 166
BspANI GGCC 5 cut(s) 5, 44, 96, 160, 188
BspCNI CTCAG 1 cut(s) 285
BspPI GGATC 1 cut(s) 332
BspTI CTTAAG 1 cut(s) 382
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 393
BssECI CCNNGG 4 cut(s) 6, 45, 75, 91
BssMI GATC 2 cut(s) 259, 324
BssT1I CCWWGG 2 cut(s) 6, 75
Bst2UI CCWGG 3 cut(s) 92, 98, 347
Bst4CI ACNGT 2 cut(s) 251, 413
BstAFI CTTAAG 1 cut(s) 382
BstDEI CTNAG 3 cut(s) 162, 272, 375
BstDSI CCRYGG 2 cut(s) 6, 45
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 3 cut(s) 16, 126, 132
BstKTI GATC 2 cut(s) 262, 327
BstMAI GTCTC 2 cut(s) 64, 275
BstMBI GATC 2 cut(s) 259, 324
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 3 cut(s) 92, 98, 347
BstSCI CCNGG 3 cut(s) 90, 96, 345
BstV1I GCAGC 4 cut(s) 27, 35, 38, 41
BstV2I GAAGAC 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 400
BsuRI GGCC 5 cut(s) 5, 44, 96, 160, 188
BtgI CCRYGG 2 cut(s) 6, 45
BtsCI GGATG 3 cut(s) 16, 126, 132
Cfr13I GGNCC 2 cut(s) 94, 158
Csp6I GTAC 1 cut(s) 396
CviAII CATG 1 cut(s) 7
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 3 cut(s) 162, 272, 375
DpnI GATC 2 cut(s) 261, 326
DpnII GATC 2 cut(s) 259, 324
DrdI GACNNNNNNGTC 1 cut(s) 411
DseDI GACNNNNNNGTC 1 cut(s) 411
EaeI YGGCCR 2 cut(s) 3, 42
Eco130I CCWWGG 2 cut(s) 6, 75
Eco57I CTGAAG 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 3 cut(s) 90, 96, 345
EcoT14I CCWWGG 2 cut(s) 6, 75
ErhI CCWWGG 2 cut(s) 6, 75
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 10
FaiI YATR 3 cut(s) 8, 195, 417
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 219, 251
FaqI GGGAC 1 cut(s) 417
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 4 cut(s) 16, 24, 27, 30
FokI GGATG 3 cut(s) 23, 113, 119
Fsp4HI GCNGC 4 cut(s) 16, 24, 27, 30
GluI GCNGC 4 cut(s) 16, 24, 27, 30
GsaI CCCAGC 1 cut(s) 404
HaeIII GGCC 5 cut(s) 5, 44, 96, 160, 188
Hin1II CATG 1 cut(s) 10
HinfI GANTC 1 cut(s) 287
HphI GGTGA 2 cut(s) 97, 331
Hpy188I TCNGA 3 cut(s) 275, 299, 329
Hpy188III TCNNGA 2 cut(s) 67, 140
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 118, 146
HpyCH4III ACNGT 2 cut(s) 251, 413
HpyCH4V TGCA 1 cut(s) 23
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 3 cut(s) 162, 272, 375
Hsp92II CATG 1 cut(s) 10
Kzo9I GATC 2 cut(s) 259, 324
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 4 cut(s) 27, 35, 38, 41
LweI GCATC 1 cut(s) 355
MaeIII GTNAC 2 cut(s) 251, 337
MalI GATC 2 cut(s) 261, 326
MbiI CCGCTC 1 cut(s) 117
MboI GATC 2 cut(s) 259, 324
MboII GAAGA 1 cut(s) 239
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 8 cut(s) 28, 130, 136, 140, 149, 277, 301, 315
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 243, 264, 383
MslI CAYNNNNRTG 1 cut(s) 420
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 32
MspCI CTTAAG 1 cut(s) 382
MspR9I CCNGG 3 cut(s) 92, 98, 347
MvaI CCWGG 3 cut(s) 92, 98, 347
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 6
NdeII GATC 2 cut(s) 259, 324
NlaIII CATG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 4 cut(s) 17, 25, 28, 31
Psp6I CCWGG 3 cut(s) 90, 96, 345
PspFI CCCAGC 1 cut(s) 400
PspGI CCWGG 3 cut(s) 90, 96, 345
PspPI GGNCC 2 cut(s) 94, 158
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
RseI CAYNNNNRTG 1 cut(s) 420
SaqAI TTAA 3 cut(s) 243, 264, 383
SatI GCNGC 4 cut(s) 16, 24, 27, 30
Sau3AI GATC 2 cut(s) 259, 324
Sau96I GGNCC 2 cut(s) 94, 158
ScrFI CCNGG 3 cut(s) 92, 98, 347
SetI ASST 8 cut(s) 77, 102, 110, 212, 226, 258, 295, 383
SfaNI GCATC 1 cut(s) 355
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 2 cut(s) 182, 382
SmoI CTYRAG 2 cut(s) 182, 382
Sse9I AATT 1 cut(s) 38
SsiI CCGC 3 cut(s) 32, 115, 166
StyD4I CCNGG 3 cut(s) 90, 96, 345
StyI CCWWGG 2 cut(s) 6, 75
TaaI ACNGT 2 cut(s) 251, 413
TaqI TCGA 2 cut(s) 111, 363
TasI AATT 1 cut(s) 38
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 3 cut(s) 243, 264, 383
Tru9I TTAA 3 cut(s) 243, 264, 383
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 4 cut(s) 15, 23, 26, 29
Tsp45I GTSAC 1 cut(s) 337
Vha464I CTTAAG 1 cut(s) 382
XagI CCTNNNNNAGG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.