Rh4AG412700

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001597
Physical Location & Seq
Forward (+)
71155107 .. 71156444
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG412700.1

Sequence Viewer

Length: 507 bp
ATGGCCATGGATGGCAGCAATGCAGCAGCAGCGGAGGAATTGGCCGTGGGTTGCTTCGTCTCCATCAAGACCACCTTGGGCGACGACTTCCAGGGCCAGGGTTTGAAAGGAGGGCCTAAGCGGAACATCAGGCTACTCAAGGCCAACTATATCAAGGAGTTGAGCTATATGGGTCAAGCTGAAGACCCACTTGACATTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCAGAGAGGAATCAGCTATCAGACAAGCAGAGGCAGAGTGTGAGAGGATCGGAGTTGGTGTCACCAGCCAGGCTCAGAACATTTTCGATGCCTTGTCTAAGACGCTTCCAGTACGCTGGGACAAGACTGTCATAGTTGTGATGAATGAGGTTCGTGTGAGCAGTCCATATCTTCCCGAGTCTGTCAGTGGAGGAACTCCTGCTGCCAATGATCGGGTGAAGAAAGTGCTTGAGTTGGAGAGGAGGAGGTTGCAAACTCGTGGTGGTGGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

168

Amino Acids

18.34

Weight (kDa)

7.65

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AD PF09793 71 - 153 3.9e-26 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AciI CCGC 2 cut(s) 32, 121
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 2 cut(s) 3, 42
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 1 cut(s) 345
AfiI CCNNNNNNNGG 2 cut(s) 97, 444
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 3 cut(s) 90, 96, 300
AluBI AGCT 3 cut(s) 165, 179, 248
AluI AGCT 3 cut(s) 165, 179, 248
Alw26I GTCTC 2 cut(s) 64, 230
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 5 cut(s) 3, 42, 94, 113, 141
ApeKI GCWGC 5 cut(s) 15, 23, 26, 29, 434
Asp700I GAANNNNTTC 1 cut(s) 314
AspS9I GGNCC 2 cut(s) 94, 113
AsuHPI GGTGA 2 cut(s) 286, 460
AvaI CYCGRG 1 cut(s) 407
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 489
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 5 cut(s) 27, 35, 38, 41, 421
BccI CCATC 2 cut(s) 5, 71
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 92, 98, 302
BcoDI GTCTC 2 cut(s) 64, 230
BisI GCNGC 5 cut(s) 16, 24, 27, 30, 435
BlsI GCNGC 5 cut(s) 17, 25, 28, 31, 436
Bme1390I CCNGG 3 cut(s) 92, 98, 302
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 94, 113
BmrFI CCNGG 3 cut(s) 92, 98, 302
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 117
BpuEI CTTGAG 2 cut(s) 122, 482
BsaJI CCNNGG 5 cut(s) 6, 45, 75, 91, 97
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 444
Bse1I ACTGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 3 cut(s) 92, 98, 302
BseDI CCNNGG 5 cut(s) 6, 45, 75, 91, 97
BseGI GGATG 1 cut(s) 16
BseLI CCNNNNNNNGG 2 cut(s) 97, 444
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 2 cut(s) 241, 320
BseNI ACTGG 1 cut(s) 341
BseRI GAGGAG 2 cut(s) 487, 490
BseXI GCAGC 5 cut(s) 27, 35, 38, 41, 421
BseYI CCCAGC 1 cut(s) 348
BshFI GGCC 5 cut(s) 5, 44, 96, 115, 143
BsiHKCI CYCGRG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 2 cut(s) 97, 444
BsmAI GTCTC 2 cut(s) 64, 230
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 5 cut(s) 5, 44, 96, 115, 143
BsoBI CYCGRG 1 cut(s) 407
Bsp143I GATC 3 cut(s) 214, 279, 442
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 2 cut(s) 32, 121
BspANI GGCC 5 cut(s) 5, 44, 96, 115, 143
BspCNI CTCAG 2 cut(s) 240, 319
BspPI GGATC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 5 cut(s) 6, 45, 75, 91, 97
BssMI GATC 3 cut(s) 214, 279, 442
BssSI CACGAG 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 6, 75
Bst2BI CACGAG 1 cut(s) 489
Bst2UI CCWGG 3 cut(s) 92, 98, 302
Bst4CI ACNGT 2 cut(s) 206, 361
BstDEI CTNAG 4 cut(s) 117, 227, 306, 330
BstDSI CCRYGG 2 cut(s) 6, 45
BstF5I GGATG 1 cut(s) 16
BstKTI GATC 3 cut(s) 217, 282, 445
BstMAI GTCTC 2 cut(s) 64, 230
BstMBI GATC 3 cut(s) 214, 279, 442
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 3 cut(s) 92, 98, 302
BstSCI CCNGG 3 cut(s) 90, 96, 300
BstV1I GCAGC 5 cut(s) 27, 35, 38, 41, 421
BstV2I GAAGAC 1 cut(s) 189
BstXI CCANNNNNNTGG 1 cut(s) 348
BsuRI GGCC 5 cut(s) 5, 44, 96, 115, 143
BtgI CCRYGG 2 cut(s) 6, 45
BtsCI GGATG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 94, 113
CseI GACGC 1 cut(s) 343
Csp6I GTAC 1 cut(s) 344
CviAII CATG 1 cut(s) 7
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 4 cut(s) 117, 227, 306, 330
DpnI GATC 3 cut(s) 216, 281, 444
DpnII GATC 3 cut(s) 214, 279, 442
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 2 cut(s) 3, 42
Eco130I CCWWGG 2 cut(s) 6, 75
Eco57I CTGAAG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 113
EcoRII CCWGG 3 cut(s) 90, 96, 300
EcoT14I CCWWGG 2 cut(s) 6, 75
ErhI CCWWGG 2 cut(s) 6, 75
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 10
FaiI YATR 6 cut(s) 8, 150, 168, 170, 365, 400
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 174, 206
FaqI GGGAC 1 cut(s) 365
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 5 cut(s) 16, 24, 27, 30, 435
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 5 cut(s) 16, 24, 27, 30, 435
GluI GCNGC 5 cut(s) 16, 24, 27, 30, 435
GsaI CCCAGC 1 cut(s) 352
HaeIII GGCC 5 cut(s) 5, 44, 96, 115, 143
HgaI GACGC 1 cut(s) 343
Hin1II CATG 1 cut(s) 10
HinfI GANTC 2 cut(s) 242, 410
HphI GGTGA 2 cut(s) 286, 460
Hpy188I TCNGA 4 cut(s) 230, 254, 284, 309
Hpy188III TCNNGA 2 cut(s) 67, 407
Hpy99I CGWCG 1 cut(s) 86
HpyCH4III ACNGT 2 cut(s) 206, 361
HpyCH4V TGCA 2 cut(s) 23, 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 4 cut(s) 117, 227, 306, 330
Hsp92II CATG 1 cut(s) 10
Kzo9I GATC 3 cut(s) 214, 279, 442
Lsp1109I GCAGC 5 cut(s) 27, 35, 38, 41, 421
LweI GCATC 1 cut(s) 310
MaeIII GTNAC 2 cut(s) 206, 292
MalI GATC 3 cut(s) 216, 281, 444
MboI GATC 3 cut(s) 214, 279, 442
MboII GAAGA 3 cut(s) 194, 395, 463
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 447
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 314
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 198, 219
MslI CAYNNNNRTG 1 cut(s) 368
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 92, 98, 302
MvaI CCWGG 3 cut(s) 92, 98, 302
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 214, 279, 442
NlaIII CATG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 5 cut(s) 17, 25, 28, 31, 436
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
Psp6I CCWGG 3 cut(s) 90, 96, 300
PspFI CCCAGC 1 cut(s) 348
PspGI CCWGG 3 cut(s) 90, 96, 300
PspPI GGNCC 2 cut(s) 94, 113
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 2 cut(s) 198, 219
SatI GCNGC 5 cut(s) 16, 24, 27, 30, 435
Sau3AI GATC 3 cut(s) 214, 279, 442
Sau96I GGNCC 2 cut(s) 94, 113
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 3 cut(s) 92, 98, 302
SetI ASST 7 cut(s) 77, 167, 181, 213, 250, 384, 482
SfaNI GCATC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 137, 461
SmoI CTYRAG 2 cut(s) 137, 461
Sse9I AATT 1 cut(s) 38
SsiI CCGC 2 cut(s) 32, 121
StyD4I CCNGG 3 cut(s) 90, 96, 300
StyI CCWWGG 2 cut(s) 6, 75
TaaI ACNGT 2 cut(s) 206, 361
TaqI TCGA 1 cut(s) 318
TasI AATT 1 cut(s) 38
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 2 cut(s) 198, 219
Tru9I TTAA 2 cut(s) 198, 219
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 5 cut(s) 15, 23, 26, 29, 434
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 1 cut(s) 389
TspRI CASTG 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.