Rorug04G0111300

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001597
Physical Location & Seq
Forward (+)
17552126 .. 17552778
653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0111300.1

Sequence Viewer

Length: 627 bp
ATGGAAACACAAACCCAGCAACCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTGTAAGCGGAGCAGGTGGAATCCCACTATAGATCAGATAAGAATCCTCAAGGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTGTAGAGCAGGTTGAGAGGATCTATCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACGTCTATTTTTGGTTCGTGAACCAAAGGGCTCGGGAGAAGCAGAAGAAGAAGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGACAATGTTACCAATTGGAAACATGAGGATCAGTATATTAACTTTGGATCTTCTGCACCTGCTTCTGCTTCTTCCGCTGGTGTGATGATTGCTGTTAACGGGCAGATGGGGAACTATGGTGGTTATGGATCTATGAACATGGAGAAGAGTGCTAGGGATTGTTCAATCTCAGCTGGAAGGGGAACTAGTTCTACTTTCTTTGACATGAATGTAGAACAAACTTTCATGGAACAAAGAGGAGAAGATCACCAGGAGATTGAAACCCTTTCACTGTTTCCCGTGCATGACTACTTCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

208

Amino Acids

23.65

Weight (kDa)

7.75

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 27 - 89 5.3e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 78, 394
Acc36I ACCTGC 3 cut(s) 78, 160, 394
AciI CCGC 2 cut(s) 82, 402
AclWI GGATC 4 cut(s) 188, 363, 382, 463
AfaI GTAC 1 cut(s) 203
AgsI TTSAA 3 cut(s) 220, 492, 587
AhlI ACTAGT 1 cut(s) 512
AjnI CCWGG 1 cut(s) 576
AluBI AGCT 3 cut(s) 130, 193, 500
AluI AGCT 3 cut(s) 130, 193, 500
AlwI GGATC 4 cut(s) 188, 363, 382, 463
Ama87I CYCGRG 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 514
AspS9I GGNCC 1 cut(s) 153
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 2 cut(s) 335, 566
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 300
BanII GRGCYC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 62
BccI CCATC 2 cut(s) 26, 427
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 578
BcnI CCSGG 1 cut(s) 55
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 2 cut(s) 480, 513
BfmI CTRYAG 2 cut(s) 102, 162
BfuAI ACCTGC 3 cut(s) 78, 160, 394
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 2 cut(s) 55, 578
Bme18I GGWCC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 1 cut(s) 155
BmrFI CCNGG 2 cut(s) 55, 578
BpmI CTGGAG 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 116
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 37, 292
BseJI GATNNNNATC 1 cut(s) 116
BseMII CTCAG 1 cut(s) 510
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 62
BseYI CCCAGC 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 2 cut(s) 256, 300
Bsp143I GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
BspACI CCGC 2 cut(s) 82, 402
BspCNI CTCAG 1 cut(s) 509
BspLI GGNNCC 1 cut(s) 155
BspMI ACCTGC 3 cut(s) 78, 160, 394
BspPI GGATC 4 cut(s) 188, 363, 382, 463
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 163, 600
Bst6I CTCTTC 1 cut(s) 467
BstDEI CTNAG 1 cut(s) 496
BstF5I GGATG 2 cut(s) 37, 292
BstKTI GATC 8 cut(s) 109, 183, 213, 311, 358, 377, 458, 574
BstMBI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
BstMWI GCNNNNNNNGC 2 cut(s) 79, 401
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 53, 576
BstSFI CTRYAG 2 cut(s) 102, 162
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 62
BstX2I RGATCY 3 cut(s) 180, 374, 455
BstYI RGATCY 3 cut(s) 180, 374, 455
BtsCI GGATG 2 cut(s) 37, 292
BtsIMutI CAGTG 1 cut(s) 596
BveI ACCTGC 3 cut(s) 78, 160, 394
Cfr13I GGNCC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 202
CviAII CATG 8 cut(s) 70, 293, 301, 350, 466, 532, 553, 611
CviJI RGCY 6 cut(s) 53, 130, 193, 254, 316, 500
CviKI_1 RGCY 6 cut(s) 53, 130, 193, 254, 316, 500
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 496
DpnI GATC 8 cut(s) 108, 182, 212, 310, 357, 376, 457, 573
DpnII GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Eco24I GRGCYC 1 cut(s) 256
Eco47I GGWCC 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 255
EcoO109I RGGNCCY 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 576
EcoT38I GRGCYC 1 cut(s) 256
FaeI CATG 8 cut(s) 73, 296, 304, 353, 469, 535, 556, 614
FaqI GGGAC 1 cut(s) 139
FatI CATG 8 cut(s) 69, 292, 300, 349, 465, 531, 552, 610
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 2 cut(s) 44, 299
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 2 cut(s) 480, 513
GluI GCNGC 1 cut(s) 51
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 173
HapII CCGG 1 cut(s) 54
Hin1II CATG 8 cut(s) 73, 296, 304, 353, 469, 535, 556, 614
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 2 cut(s) 94, 117
HpaI GTTAAC 1 cut(s) 424
HpaII CCGG 1 cut(s) 54
HphI GGTGA 2 cut(s) 335, 566
Hpy166II GTNNAC 3 cut(s) 244, 279, 424
Hpy188I TCNGA 3 cut(s) 111, 286, 623
Hpy188III TCNNGA 3 cut(s) 220, 241, 257
Hpy8I GTNNAC 3 cut(s) 244, 279, 424
HpyAV CCTTC 1 cut(s) 498
HpyCH4III ACNGT 2 cut(s) 163, 600
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 304, 383, 610
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 401
HpyF3I CTNAG 1 cut(s) 496
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 8 cut(s) 73, 296, 304, 353, 469, 535, 556, 614
KspAI GTTAAC 1 cut(s) 424
Kzo9I GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
LmnI GCTCC 2 cut(s) 84, 127
Lsp1109I GCAGC 1 cut(s) 62
MaeI CTAG 2 cut(s) 480, 513
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 334
MalI GATC 8 cut(s) 108, 182, 212, 310, 357, 376, 457, 573
MboI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
MboII GAAGA 6 cut(s) 280, 283, 369, 390, 484, 581
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 3 cut(s) 180, 374, 455
MhlI GDGCHC 2 cut(s) 256, 300
MluCI AATT 1 cut(s) 340
MnlI CCTC 6 cut(s) 22, 29, 131, 171, 346, 557
MroXI GAANNNNTTC 1 cut(s) 514
MseI TTAA 2 cut(s) 366, 423
MspA1I CMGCKG 3 cut(s) 193, 404, 500
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 2 cut(s) 55, 578
MunI CAATTG 1 cut(s) 340
MvaI CCWGG 1 cut(s) 578
MwoI GCNNNNNNNGC 2 cut(s) 79, 401
NciI CCSGG 1 cut(s) 55
NdeII GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
NlaIII CATG 8 cut(s) 73, 296, 304, 353, 469, 535, 556, 614
NlaIV GGNNCC 1 cut(s) 155
NspI RCATGY 1 cut(s) 73
PaqCI CACCTGC 2 cut(s) 78, 394
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 514
PfeI GAWTC 2 cut(s) 94, 117
PkrI GCNGC 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 153
Psp5II RGGWCCY 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 576
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 1 cut(s) 155
PspPI GGNCC 1 cut(s) 153
PspPPI RGGWCCY 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 502, 534
PsuI RGATCY 3 cut(s) 180, 374, 455
PvuII CAGCTG 2 cut(s) 193, 500
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SaqAI TTAA 2 cut(s) 366, 423
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 8 cut(s) 106, 180, 210, 308, 355, 374, 455, 571
Sau96I GGNCC 1 cut(s) 153
ScrFI CCNGG 2 cut(s) 55, 578
SduI GDGCHC 2 cut(s) 256, 300
SetI ASST 8 cut(s) 92, 132, 155, 174, 195, 228, 388, 502
SfcI CTRYAG 2 cut(s) 102, 162
SinI GGWCC 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 1 cut(s) 340
SsiI CCGC 2 cut(s) 82, 402
SspMI CTAG 2 cut(s) 480, 513
StyD4I CCNGG 2 cut(s) 53, 576
TaaI ACNGT 2 cut(s) 163, 600
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 213
TasI AATT 1 cut(s) 340
TfiI GAWTC 2 cut(s) 94, 117
Tru1I TTAA 2 cut(s) 366, 423
Tru9I TTAA 2 cut(s) 366, 423
TscAI CASTG 1 cut(s) 603
TseI GCWGC 1 cut(s) 50
TspDTI ATGAA 4 cut(s) 281, 476, 541, 548
TspRI CASTG 1 cut(s) 603
VpaK11BI GGWCC 1 cut(s) 153
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 514
XspI CTAG 2 cut(s) 480, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.