RchiOBHm_Chr4g0444381

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001597
Physical Location & Seq
Forward (+)
65140526 .. 65141995
1470 bp
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UTR
Exon/CDS
Intron
PRQ41207

Sequence Viewer

Length: 339 bp
ATGGGTCAAGCTGAAGACCCACTTGACATTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCCGAGAGGAATCAGCTATCAGACAAGCAGAGGCAGAGTGTGAGAGGATCGGAGTTGGTGTCACCAGCCAGGCTCAGAACATTTTCGATGCCTTGTCTAAGACGCTTCCAGTACGGTGGGACAAGACTGTCATAGTTGTGATGAATGAGGTTCGTGTGAGCAGTCCATATCTTCCCGAGTCTGTCAGCGGAGGAACTCCTGCTGCCAATGATCGGGTGAAGAAAGTGCTTGAGTTGGAGAGGAGGAGGTTGCAAACTCGTGGTGGTGGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

112

Amino Acids

12.4

Weight (kDa)

6.3

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AD PF09793 15 - 97 1.2e-26 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AciI CCGC 1 cut(s) 252
AclWI GGATC 1 cut(s) 119
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 276
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 2 cut(s) 11, 80
AluI AGCT 2 cut(s) 11, 80
Alw26I GTCTC 1 cut(s) 62
AlwI GGATC 1 cut(s) 119
Ama87I CYCGRG 2 cut(s) 66, 239
ApeKI GCWGC 1 cut(s) 266
Asp700I GAANNNNTTC 1 cut(s) 146
AsuHPI GGTGA 2 cut(s) 118, 292
AvaI CYCGRG 2 cut(s) 66, 239
BanII GRGCYC 1 cut(s) 67
BauI CACGAG 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 1 cut(s) 253
BciT130I CCWGG 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 62
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 1 cut(s) 134
BmeT110I CYCGRG 2 cut(s) 66, 239
BmrFI CCNGG 1 cut(s) 134
BmsI GCATC 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 314
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 134
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMII CTCAG 2 cut(s) 73, 152
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 2 cut(s) 319, 322
BseXI GCAGC 1 cut(s) 253
BsiHKCI CYCGRG 2 cut(s) 66, 239
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 197
BsoBI CYCGRG 2 cut(s) 66, 239
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 3 cut(s) 46, 111, 274
BspACI CCGC 1 cut(s) 252
BspCNI CTCAG 2 cut(s) 72, 151
BspPI GGATC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 173
BssMI GATC 3 cut(s) 46, 111, 274
BssSI CACGAG 1 cut(s) 321
Bst2BI CACGAG 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 3 cut(s) 38, 180, 193
BstDEI CTNAG 3 cut(s) 59, 138, 162
BstKTI GATC 3 cut(s) 49, 114, 277
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 3 cut(s) 46, 111, 274
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 132
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 21
BstXI CCANNNNNNTGG 1 cut(s) 180
CseI GACGC 1 cut(s) 175
Csp6I GTAC 1 cut(s) 176
CviJI RGCY 5 cut(s) 11, 65, 80, 132, 137
CviKI_1 RGCY 5 cut(s) 11, 65, 80, 132, 137
CviQI GTAC 1 cut(s) 176
DdeI CTNAG 3 cut(s) 59, 138, 162
DpnI GATC 3 cut(s) 48, 113, 276
DpnII GATC 3 cut(s) 46, 111, 274
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 33
Eco88I CYCGRG 2 cut(s) 66, 239
EcoRII CCWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 67
FaiI YATR 2 cut(s) 197, 232
FalI AAGNNNNNCTT 1 cut(s) 38
FaqI GGGAC 1 cut(s) 197
Fnu4HI GCNGC 1 cut(s) 267
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 267
GluI GCNGC 1 cut(s) 267
HgaI GACGC 1 cut(s) 175
HinfI GANTC 2 cut(s) 74, 242
HphI GGTGA 2 cut(s) 118, 292
Hpy188I TCNGA 4 cut(s) 62, 86, 116, 141
Hpy188III TCNNGA 1 cut(s) 239
HpyCH4III ACNGT 3 cut(s) 38, 180, 193
HpyCH4V TGCA 1 cut(s) 316
HpyF3I CTNAG 3 cut(s) 59, 138, 162
Kzo9I GATC 3 cut(s) 46, 111, 274
LpnPI CCDG 5 cut(s) 119, 142, 146, 186, 276
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 1 cut(s) 142
MaeIII GTNAC 2 cut(s) 38, 124
MalI GATC 3 cut(s) 48, 113, 276
MboI GATC 3 cut(s) 46, 111, 274
MboII GAAGA 3 cut(s) 26, 227, 295
MhlI GDGCHC 1 cut(s) 67
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 8 cut(s) 64, 88, 102, 205, 248, 297, 300, 303
MroXI GAANNNNTTC 1 cut(s) 146
MseI TTAA 2 cut(s) 30, 51
MslI CAYNNNNRTG 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 134
MvaI CCWGG 1 cut(s) 134
NdeII GATC 3 cut(s) 46, 111, 274
NmuCI GTSAC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 1 cut(s) 74
PkrI GCNGC 1 cut(s) 268
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
RsaI GTAC 1 cut(s) 177
RsaNI GTAC 1 cut(s) 176
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 2 cut(s) 30, 51
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 3 cut(s) 46, 111, 274
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 1 cut(s) 67
SetI ASST 5 cut(s) 13, 45, 82, 216, 314
SfaNI GCATC 1 cut(s) 142
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SsiI CCGC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 38, 180, 193
TaqI TCGA 1 cut(s) 150
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 2 cut(s) 30, 51
Tru9I TTAA 2 cut(s) 30, 51
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.