Rorug04G0352300

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001597
Physical Location & Seq
Reverse (-)
52408363 .. 52431353
22991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0352300.1

Sequence Viewer

Length: 1104 bp
ATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATACTAGGTGAGCCAACCGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCTTCCAACGAAGAAAAGCTCTTTGTAGCATGGAGACCTCACTCAATGCAAAAGCTCTTCCGCTCTTCTTACCAAAAAGCTGCTTCCACCGTCATACCAAGACAAATCGAAAAGCACGTAGTTCGGGCACCGGGGTTTCTGAGAGCCGAAGCCCTGGTTGGCTACGTGGGAGGGATGGATTCCATGGTTCTCGACCACCTGAAAACACACTGGGATGGCAAACAGGTGGTCGAGGCTCATCGCCTCACGCAGCTACTCGTTTTGACTCTCTCGGCTAAGTTCTTCATGGGACTCGAAGACCATTGTCGTATTGATAAGCTTGCGGAGTTGATGGACACCATGATGCTGGCGCTTCACACTGTTCCTGTGAATATTCCGGGAACTGCATTTTATCAAGCAATGAAAGCAGCGCAGGCTGTGAGGGAGACTATTCAGTCGTTTATTAAGGAGAAGAAGGAAGTTATGGCTTCTACCGGAAACAAAATGCATGATCTTTTGTCTTATTTGATTGCTACGGCAGACCCGGTTACCGGAAGGTTCATGCCGGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCGTTCAACTCTCCAGCTATGACTACTACTTTTATCGTGAAGTTCCTGGGCGAAAGACCTGACATTTATGACAAAGTTCGAATTGAACAACTGGAGATTTTGAAGTCCAAAAAATCTGGCGAGGCACTCAATTGGGATGATATGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACGAAGGTTTTACAATTCCTAAAGGATGGAAGGTGTATTGGTCGGCTAGCAGCACAAATAAAGTCTCCGAGTATTTTCCAGCACCAGAAAAGTTCGATCCAACCAGGTTCGAGAATGGAAAAGCTCCCCCTGCTTACTCGAACATCCCATTTGGAAGCGGACCTCGAATTTGTCCAGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

367

Amino Acids

41.25

Weight (kDa)

9.28

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 367 1.9e-44 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 523
AccB1I GGYRCC 1 cut(s) 217
AccBSI CCGCTC 1 cut(s) 153
AciI CCGC 4 cut(s) 151, 413, 675, 1077
AclWI GGATC 1 cut(s) 1010
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 2 cut(s) 83, 1086
AcuI CTGAAG 1 cut(s) 866
AfiI CCNNNNNNNGG 2 cut(s) 620, 884
AgsI TTSAA 4 cut(s) 28, 685, 764, 781
AjnI CCWGG 5 cut(s) 243, 723, 852, 1022, 1093
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 7 cut(s) 109, 145, 170, 343, 409, 695, 1043
AluI AGCT 7 cut(s) 109, 145, 170, 343, 409, 695, 1043
Alw26I GTCTC 3 cut(s) 118, 509, 988
AlwI GGATC 1 cut(s) 1010
AoxI GGCC 1 cut(s) 672
ApeKI GCWGC 5 cut(s) 170, 340, 497, 902, 969
ApoI RAATTY 2 cut(s) 83, 1086
ArsI GACNNNNNNTTYG 4 cut(s) 303, 335, 1071, 1103
AspLEI GCGC 2 cut(s) 442, 502
AspS9I GGNCC 1 cut(s) 1079
AsuC2I CCSGG 3 cut(s) 222, 468, 614
AsuHPI GGTGA 1 cut(s) 56
AsuII TTCGAA 1 cut(s) 757
AsuNHI GCTAGC 1 cut(s) 965
AvaII GGWCC 1 cut(s) 1079
BaeGI GKGCMC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 393
BbvI GCAGC 5 cut(s) 157, 352, 509, 889, 981
BccI CCATC 4 cut(s) 259, 299, 415, 939
BceAI ACGGC 1 cut(s) 621
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 5 cut(s) 245, 725, 854, 1024, 1095
BcnI CCSGG 3 cut(s) 222, 468, 614
BcoDI GTCTC 3 cut(s) 118, 509, 988
BfaI CTAG 2 cut(s) 41, 966
BfoI RGCGCY 1 cut(s) 443
BglI GCCNNNNNGGC 1 cut(s) 857
BisI GCNGC 6 cut(s) 171, 341, 498, 675, 903, 970
BlsI GCNGC 6 cut(s) 172, 342, 499, 676, 904, 971
Bme1390I CCNGG 8 cut(s) 222, 245, 468, 614, 725, 854, 1024, 1095
Bme18I GGWCC 1 cut(s) 1079
BmgT120I GGNCC 1 cut(s) 1079
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 8 cut(s) 222, 245, 468, 614, 725, 854, 1024, 1095
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 1 cut(s) 423
BmtI GCTAGC 1 cut(s) 969
BmuI ACTGGG 1 cut(s) 310
BoxI GACNNNNGTC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 393
BpmI CTGGAG 3 cut(s) 675, 791, 875
Bpu14I TTCGAA 1 cut(s) 757
BpuMI CCSGG 3 cut(s) 222, 468, 614
BsaAI YACGTR 2 cut(s) 208, 256
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 6 cut(s) 54, 221, 243, 273, 724, 852
BsaWI WCCGGW 2 cut(s) 563, 620
Bsc4I CCNNNNNNNGG 2 cut(s) 620, 884
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 305, 774
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 5 cut(s) 245, 725, 854, 1024, 1095
BseDI CCNNGG 6 cut(s) 54, 221, 243, 273, 724, 852
BseGI GGATG 5 cut(s) 270, 310, 820, 950, 1062
BseLI CCNNNNNNNGG 2 cut(s) 620, 884
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 2 cut(s) 305, 774
BseRI GAGGAG 1 cut(s) 4
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 5 cut(s) 157, 352, 509, 889, 981
BshFI GGCC 1 cut(s) 674
BshNI GGYRCC 1 cut(s) 217
BsiSI CCGG 7 cut(s) 75, 221, 467, 564, 614, 621, 635
BslFI GGGAC 1 cut(s) 393
BslI CCNNNNNNNGG 2 cut(s) 620, 884
BsmAI GTCTC 3 cut(s) 118, 509, 988
BsmFI GGGAC 1 cut(s) 393
BsnI GGCC 1 cut(s) 674
Bso31I GGTCTC 1 cut(s) 118
Bsp119I TTCGAA 1 cut(s) 757
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 2 cut(s) 580, 1015
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 4 cut(s) 151, 413, 675, 1077
BspANI GGCC 1 cut(s) 674
BspCNI CTCAG 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 219
BspOI GCTAGC 1 cut(s) 969
BspPI GGATC 1 cut(s) 1010
BspQI GCTCTTC 2 cut(s) 152, 160
BspT104I TTCGAA 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 118
BsrBI CCGCTC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 2 cut(s) 305, 774
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 6 cut(s) 54, 221, 243, 273, 724, 852
BssMI GATC 2 cut(s) 580, 1015
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 5 cut(s) 245, 725, 854, 1024, 1095
Bst4CI ACNGT 3 cut(s) 55, 181, 451
Bst6I CTCTTC 3 cut(s) 152, 160, 885
BstBAI YACGTR 2 cut(s) 208, 256
BstBI TTCGAA 1 cut(s) 757
BstC8I GCNNGC 4 cut(s) 411, 438, 504, 967
BstDEI CTNAG 2 cut(s) 230, 366
BstDSI CCRYGG 2 cut(s) 54, 273
BstEII GGTNACC 1 cut(s) 616
BstF5I GGATG 5 cut(s) 270, 310, 820, 950, 1062
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 2 cut(s) 442, 502
BstKTI GATC 2 cut(s) 583, 1018
BstMAI GTCTC 3 cut(s) 118, 509, 988
BstMBI GATC 2 cut(s) 580, 1015
BstMWI GCNNNNNNNGC 4 cut(s) 494, 503, 857, 1049
BstNI CCWGG 5 cut(s) 245, 725, 854, 1024, 1095
BstPAI GACNNNNGTC 1 cut(s) 393
BstPI GGTNACC 1 cut(s) 616
BstSCI CCNGG 8 cut(s) 220, 243, 466, 612, 723, 852, 1022, 1093
BstSLI GKGCMC 1 cut(s) 220
BstV1I GCAGC 5 cut(s) 157, 352, 509, 889, 981
BstV2I GAAGAC 1 cut(s) 393
BstXI CCANNNNNNTGG 1 cut(s) 436
BsuRI GGCC 1 cut(s) 674
BtgI CCRYGG 2 cut(s) 54, 273
BtgZI GCGATG 2 cut(s) 314, 875
BtsCI GGATG 5 cut(s) 270, 310, 820, 950, 1062
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 3 cut(s) 69, 298, 447
Cac8I GCNNGC 4 cut(s) 411, 438, 504, 967
CfoI GCGC 2 cut(s) 442, 502
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 1079
CsiI ACCWGGT 1 cut(s) 1022
CviAII CATG 6 cut(s) 120, 274, 376, 430, 578, 631
DdeI CTNAG 2 cut(s) 230, 366
DpnI GATC 2 cut(s) 582, 1017
DpnII GATC 2 cut(s) 580, 1015
DrdI GACNNNNNNGTC 1 cut(s) 523
DseDI GACNNNNNNGTC 1 cut(s) 523
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 3 cut(s) 152, 160, 885
EarI CTCTTC 3 cut(s) 152, 160, 885
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 1079
Eco57I CTGAAG 1 cut(s) 866
Eco91I GGTNACC 1 cut(s) 616
EcoO65I GGTNACC 1 cut(s) 616
EcoRII CCWGG 5 cut(s) 243, 723, 852, 1022, 1093
EcoT14I CCWWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 6 cut(s) 123, 277, 379, 433, 581, 634
FaqI GGGAC 1 cut(s) 393
FatI CATG 6 cut(s) 119, 273, 375, 429, 577, 630
Fnu4HI GCNGC 6 cut(s) 171, 341, 498, 675, 903, 970
FokI GGATG 5 cut(s) 277, 317, 827, 957, 1049
Fsp4HI GCNGC 6 cut(s) 171, 341, 498, 675, 903, 970
FspBI CTAG 2 cut(s) 41, 966
GlaI GCGC 2 cut(s) 441, 501
GluI GCNGC 6 cut(s) 171, 341, 498, 675, 903, 970
GsuI CTGGAG 3 cut(s) 675, 791, 875
HaeII RGCGCY 1 cut(s) 443
HaeIII GGCC 1 cut(s) 674
HapII CCGG 7 cut(s) 75, 221, 467, 564, 614, 621, 635
HhaI GCGC 2 cut(s) 442, 502
Hin1II CATG 6 cut(s) 123, 277, 379, 433, 581, 634
Hin6I GCGC 2 cut(s) 440, 500
HinP1I GCGC 2 cut(s) 440, 500
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 3 cut(s) 269, 355, 381
HpaII CCGG 7 cut(s) 75, 221, 467, 564, 614, 621, 635
HphI GGTGA 1 cut(s) 56
Hpy188I TCNGA 2 cut(s) 231, 988
Hpy188III TCNNGA 3 cut(s) 281, 715, 1030
HpyAV CCTTC 4 cut(s) 538, 618, 917, 943
HpyCH4III ACNGT 3 cut(s) 55, 181, 451
HpyCH4IV ACGT 3 cut(s) 207, 255, 846
HpyCH4V TGCA 5 cut(s) 139, 476, 577, 823, 905
HpyF10VI GCNNNNNNNGC 4 cut(s) 494, 503, 857, 1049
HpyF3I CTNAG 2 cut(s) 230, 366
HpySE526I ACGT 3 cut(s) 207, 255, 846
Hsp92II CATG 6 cut(s) 123, 277, 379, 433, 581, 634
HspAI GCGC 2 cut(s) 440, 500
Kzo9I GATC 2 cut(s) 580, 1015
LguI GCTCTTC 2 cut(s) 152, 160
LmnI GCTCC 1 cut(s) 1048
Lsp1109I GCAGC 5 cut(s) 157, 352, 509, 889, 981
LweI GCATC 1 cut(s) 423
MabI ACCWGGT 1 cut(s) 1022
MaeI CTAG 2 cut(s) 41, 966
MaeII ACGT 3 cut(s) 207, 255, 846
MaeIII GTNAC 2 cut(s) 77, 616
MalI GATC 2 cut(s) 582, 1017
MbiI CCGCTC 1 cut(s) 153
MboI GATC 2 cut(s) 580, 1015
MboII GAAGA 9 cut(s) 16, 16, 113, 139, 147, 364, 398, 553, 872
MfeI CAATTG 1 cut(s) 808
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 7 cut(s) 83, 645, 657, 759, 808, 933, 1086
MlyI GAGTC 2 cut(s) 349, 375
MmeI TCCRAC 2 cut(s) 120, 1043
Mph1103I ATGCAT 1 cut(s) 579
MseI TTAA 1 cut(s) 534
MslI CAYNNNNRTG 2 cut(s) 303, 431
MspI CCGG 7 cut(s) 75, 221, 467, 564, 614, 621, 635
MspR9I CCNGG 8 cut(s) 222, 245, 468, 614, 725, 854, 1024, 1095
MunI CAATTG 1 cut(s) 808
MvaI CCWGG 5 cut(s) 245, 725, 854, 1024, 1095
MwoI GCNNNNNNNGC 4 cut(s) 494, 503, 857, 1049
NciI CCSGG 3 cut(s) 222, 468, 614
NcoI CCATGG 1 cut(s) 273
NdeII GATC 2 cut(s) 580, 1015
NheI GCTAGC 1 cut(s) 965
NlaIII CATG 6 cut(s) 123, 277, 379, 433, 581, 634
NlaIV GGNNCC 1 cut(s) 219
NmeAIII GCCGAG 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 77
NsiI ATGCAT 1 cut(s) 579
NspV TTCGAA 1 cut(s) 757
PciSI GCTCTTC 2 cut(s) 152, 160
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 1 cut(s) 269
PfoI TCCNGGA 2 cut(s) 466, 1093
PkrI GCNGC 6 cut(s) 172, 342, 499, 676, 904, 971
PleI GAGTC 2 cut(s) 349, 375
PpsI GAGTC 2 cut(s) 349, 375
Ppu21I YACGTR 2 cut(s) 208, 256
PshAI GACNNNNGTC 1 cut(s) 393
Psp6I CCWGG 5 cut(s) 243, 723, 852, 1022, 1093
PspEI GGTNACC 1 cut(s) 616
PspGI CCWGG 5 cut(s) 243, 723, 852, 1022, 1093
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 1079
RseI CAYNNNNRTG 2 cut(s) 303, 431
SapI GCTCTTC 2 cut(s) 152, 160
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 6 cut(s) 171, 341, 498, 675, 903, 970
Sau3AI GATC 2 cut(s) 580, 1015
Sau96I GGNCC 1 cut(s) 1079
SchI GAGTC 2 cut(s) 349, 375
ScrFI CCNGG 8 cut(s) 222, 245, 468, 614, 725, 854, 1024, 1095
SduI GDGCHC 1 cut(s) 220
SexAI ACCWGGT 1 cut(s) 1022
SfaNI GCATC 1 cut(s) 423
SfuI TTCGAA 1 cut(s) 757
SinI GGWCC 1 cut(s) 1079
SmiMI CAYNNNNRTG 2 cut(s) 303, 431
Sse9I AATT 7 cut(s) 83, 645, 657, 759, 808, 933, 1086
SsiI CCGC 4 cut(s) 151, 413, 675, 1077
SspI AATATT 1 cut(s) 463
SspMI CTAG 2 cut(s) 41, 966
StyD4I CCNGG 8 cut(s) 220, 243, 466, 612, 723, 852, 1022, 1093
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 3 cut(s) 55, 181, 451
TaiI ACGT 3 cut(s) 210, 258, 849
TaqI TCGA 9 cut(s) 198, 282, 321, 384, 757, 1014, 1029, 1058, 1084
TasI AATT 7 cut(s) 83, 645, 657, 759, 808, 933, 1086
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 3 cut(s) 76, 305, 454
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 5 cut(s) 170, 340, 497, 902, 969
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 6 cut(s) 17, 364, 506, 619, 657, 845
TspRI CASTG 3 cut(s) 76, 305, 454
VpaK11BI GGWCC 1 cut(s) 1079
XapI RAATTY 2 cut(s) 83, 1086
XspI CTAG 2 cut(s) 41, 966
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.