Prupe.1G258500_v2.0.a1

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001597
Physical Location & Seq
Forward (+)
26817334 .. 26817989
656 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G258500.1

Sequence Viewer

Length: 375 bp
AACACTCCAATTTGGGGAAAAACTAAAAAAATAAGGGGCATGGCCATGGATGGCAGCAACGCGGAGGAGTTGGCCATTGGGTGCTTCGTCTCCATCAAGACCACCTTGAGTGACGAGTTTCAGGGATTACAAGAGGGTTCGAAAGGAGGGTCTCGGCGGAACATAAGGCTGCTGAAGGCGAACTATATAAAGGAGTTGTCGTATTTGGGTCAAGCCGAAGACCCACTTGATGTCAAAAACTGTTACCTTGATCTCAATAGTCTCCGCGCCAGAGAGGAATTGGCCATTAGACAAGCAGAGGCAGAGTCCGAGAGGATTGGTGTTGGGGTCACCAGCCAGGCCCAGAACATTTTCGACGCCTTGTCTAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

125

Amino Acids

13.65

Weight (kDa)

6.54

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 62, 267
AciI CCGC 3 cut(s) 62, 157, 265
AcoI YGGCCR 3 cut(s) 42, 72, 282
AcuI CTGAAG 1 cut(s) 194
AcyI GRCGYC 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 14
AhdI GACNNNNNGTC 1 cut(s) 361
AjiI CACGTC 1 cut(s) 372
AjnI CCWGG 1 cut(s) 336
Alw26I GTCTC 3 cut(s) 94, 156, 266
AoxI GGCC 4 cut(s) 42, 72, 282, 339
ApeKI GCWGC 2 cut(s) 54, 169
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 340
AsuHPI GGTGA 1 cut(s) 322
AsuII TTCGAA 1 cut(s) 140
BalI TGGCCA 3 cut(s) 44, 74, 284
BbsI GAAGAC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 66, 156
BccI CCATC 2 cut(s) 44, 101
BciT130I CCWGG 1 cut(s) 338
BcoDI GTCTC 3 cut(s) 94, 156, 266
BisI GCNGC 2 cut(s) 55, 170
BlsI GCNGC 2 cut(s) 56, 171
Bme1390I CCNGG 1 cut(s) 338
BmeRI GACNNNNNGTC 1 cut(s) 361
BmgBI CACGTC 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 340
BmrFI CCNGG 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 2 cut(s) 66, 156
Bsh1236I CGCG 2 cut(s) 62, 267
BshFI GGCC 4 cut(s) 44, 74, 284, 341
BslI CCNNNNNNNGG 1 cut(s) 14
BsmAI GTCTC 3 cut(s) 94, 156, 266
BsmBI CGTCTC 1 cut(s) 94
BsnI GGCC 4 cut(s) 44, 74, 284, 341
Bso31I GGTCTC 1 cut(s) 156
Bsp119I TTCGAA 1 cut(s) 140
Bsp143I GATC 1 cut(s) 250
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 3 cut(s) 62, 157, 265
BspANI GGCC 4 cut(s) 44, 74, 284, 341
BspFNI CGCG 2 cut(s) 62, 267
BspT104I TTCGAA 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 156
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 1 cut(s) 250
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 1 cut(s) 242
BstACI GRCGYC 1 cut(s) 357
BstBI TTCGAA 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 366
BstDSI CCRYGG 1 cut(s) 45
BstEII GGTNACC 1 cut(s) 328
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 2 cut(s) 62, 267
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 1 cut(s) 253
BstMAI GTCTC 3 cut(s) 94, 156, 266
BstMBI GATC 1 cut(s) 250
BstNI CCWGG 1 cut(s) 338
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 336
BstUI CGCG 2 cut(s) 62, 267
BstV1I GCAGC 2 cut(s) 66, 156
BstV2I GAAGAC 1 cut(s) 225
BsuRI GGCC 4 cut(s) 44, 74, 284, 341
BtgI CCRYGG 1 cut(s) 45
BtrI CACGTC 1 cut(s) 372
BtsCI GGATG 1 cut(s) 55
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 340
CseI GACGC 1 cut(s) 365
CviAII CATG 2 cut(s) 40, 46
CviJI RGCY 7 cut(s) 44, 74, 169, 215, 284, 336, 341
CviKI_1 RGCY 7 cut(s) 44, 74, 169, 215, 284, 336, 341
DdeI CTNAG 1 cut(s) 366
DpnI GATC 1 cut(s) 252
DpnII GATC 1 cut(s) 250
DriI GACNNNNNGTC 1 cut(s) 361
EaeI YGGCCR 3 cut(s) 42, 72, 282
Eam1105I GACNNNNNGTC 1 cut(s) 361
EciI GGCGGA 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 194
Eco91I GGTNACC 1 cut(s) 328
EcoO65I GGTNACC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 2 cut(s) 43, 49
FaiI YATR 5 cut(s) 41, 47, 164, 186, 188
FalI AAGNNNNNCTT 4 cut(s) 89, 121, 210, 242
FatI CATG 2 cut(s) 39, 45
Fnu4HI GCNGC 2 cut(s) 55, 170
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 2 cut(s) 55, 170
GlaI GCGC 1 cut(s) 268
GluI GCNGC 2 cut(s) 55, 170
HaeIII GGCC 4 cut(s) 44, 74, 284, 341
HgaI GACGC 1 cut(s) 365
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 2 cut(s) 43, 49
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HinfI GANTC 1 cut(s) 305
HphI GGTGA 1 cut(s) 322
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 1 cut(s) 97
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 371
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 371
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 2 cut(s) 43, 49
HspAI GCGC 1 cut(s) 267
Kzo9I GATC 1 cut(s) 250
LpnPI CCDG 6 cut(s) 107, 283, 323, 346, 350, 356
Lsp1109I GCAGC 2 cut(s) 66, 156
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 3 cut(s) 110, 242, 328
MalI GATC 1 cut(s) 252
MboI GATC 1 cut(s) 250
MboII GAAGA 1 cut(s) 230
MlsI TGGCCA 3 cut(s) 44, 74, 284
MluCI AATT 2 cut(s) 9, 278
MluNI TGGCCA 3 cut(s) 44, 74, 284
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 6 cut(s) 58, 127, 140, 268, 292, 306
Mox20I TGGCCA 3 cut(s) 44, 74, 284
MroXI GAANNNNTTC 1 cut(s) 350
MscI TGGCCA 3 cut(s) 44, 74, 284
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 3 cut(s) 44, 74, 284
MspR9I CCNGG 1 cut(s) 338
MvaI CCWGG 1 cut(s) 338
MvnI CGCG 2 cut(s) 62, 267
NcoI CCATGG 1 cut(s) 45
NdeII GATC 1 cut(s) 250
NlaIII CATG 2 cut(s) 43, 49
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 2 cut(s) 110, 328
NspV TTCGAA 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 350
PkrI GCNGC 2 cut(s) 56, 171
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 336
PspEI GGTNACC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 336
PspPI GGNCC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 44
SatI GCNGC 2 cut(s) 55, 170
Sau3AI GATC 1 cut(s) 250
Sau96I GGNCC 1 cut(s) 340
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 1 cut(s) 338
SetI ASST 3 cut(s) 107, 249, 374
SfuI TTCGAA 1 cut(s) 140
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 106
SmoI CTYRAG 1 cut(s) 106
Sse9I AATT 2 cut(s) 9, 278
SsiI CCGC 3 cut(s) 62, 157, 265
StyD4I CCNGG 1 cut(s) 336
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 374
TaqI TCGA 2 cut(s) 140, 354
TasI AATT 2 cut(s) 9, 278
TseFI GTSAC 2 cut(s) 110, 328
TseI GCWGC 2 cut(s) 54, 169
Tsp45I GTSAC 2 cut(s) 110, 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 277
XmnI GAANNNNTTC 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.