Rroxscaffold_5G00384910

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001597
Physical Location & Seq
Forward (+)
64126682 .. 64128400
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384910.1

Sequence Viewer

Length: 555 bp
ATGGCCATGGATGGCAGCAATGCAGCAGCAGCGGAGGAATTGGCCGTGGGTTGCTTCGTCTCCATCAAGACCACCTTGGGCGACGACTTCCAGGGCCAGGTCATCACCTTCGACCGCCCCTCCAACATCCTCATCCTTCAAGAGGGTTTGAAAGGAGGGCCTAAGCGGAACATCAGGCTACTCAAGGCCAACTACATCAAGGAGTTGAGCTATTTGGGTCAAGCTGAAGACCCACTTGACATTAAGAACTGTTATCTTGATCTTAATAGTCTCAGGGCCAGAGAGGAATCAGCTATCAGACAAGCAGAGGCAGAGTGTGAGAGGATCGGGGTCGGTGTCACCAGCCAGGCTCAGAACATTTTCGATGCCTTGTCTAAGACGCTTCCAGTACGCTGGGACAAGACTGTCATAGTTGTGATGAATGAGGTTCGTGTGAGCAGTCCATATCTTCCCGAGTCTGTCAGTGGAGGAACTCCTGCTGCCAATGATCGGGTGAAGAAAGTGCTTGAGTTGGAGAGGAGGAGGTTGCAAACTCGTGGTGGTGGTGGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

184

Amino Acids

20.12

Weight (kDa)

6.39

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM12_LSM PF21166 12 - 68 2.3e-13 LSM12, LSM domain
AD PF09793 86 - 168 4.8e-26 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
AciI CCGC 3 cut(s) 32, 115, 166
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 2 cut(s) 3, 42
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 390
AfiI CCNNNNNNNGG 3 cut(s) 97, 142, 489
AgsI TTSAA 2 cut(s) 140, 151
AjnI CCWGG 3 cut(s) 90, 96, 345
AluBI AGCT 3 cut(s) 210, 224, 293
AluI AGCT 3 cut(s) 210, 224, 293
Alw26I GTCTC 2 cut(s) 64, 275
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 6 cut(s) 3, 42, 94, 158, 186, 276
ApeKI GCWGC 5 cut(s) 15, 23, 26, 29, 479
Asp700I GAANNNNTTC 1 cut(s) 359
AspS9I GGNCC 3 cut(s) 94, 158, 276
AsuHPI GGTGA 3 cut(s) 97, 331, 505
AvaI CYCGRG 1 cut(s) 452
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 534
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 5 cut(s) 27, 35, 38, 41, 466
BccI CCATC 2 cut(s) 5, 71
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 92, 98, 347
BcoDI GTCTC 2 cut(s) 64, 275
BisI GCNGC 5 cut(s) 16, 24, 27, 30, 480
BlsI GCNGC 5 cut(s) 17, 25, 28, 31, 481
Bme1390I CCNGG 3 cut(s) 92, 98, 347
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 3 cut(s) 94, 158, 276
BmrFI CCNGG 3 cut(s) 92, 98, 347
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 2 cut(s) 167, 527
BsaJI CCNNGG 4 cut(s) 6, 45, 75, 91
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 142, 489
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 3 cut(s) 92, 98, 347
BseDI CCNNGG 4 cut(s) 6, 45, 75, 91
BseGI GGATG 3 cut(s) 16, 126, 132
BseLI CCNNNNNNNGG 3 cut(s) 97, 142, 489
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 2 cut(s) 286, 365
BseNI ACTGG 1 cut(s) 386
BseRI GAGGAG 2 cut(s) 532, 535
BseXI GCAGC 5 cut(s) 27, 35, 38, 41, 466
BseYI CCCAGC 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 6 cut(s) 5, 44, 96, 160, 188, 278
BsiEI CGRYCG 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 3 cut(s) 97, 142, 489
BsmAI GTCTC 2 cut(s) 64, 275
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 6 cut(s) 5, 44, 96, 160, 188, 278
BsoBI CYCGRG 1 cut(s) 452
Bsp143I GATC 3 cut(s) 259, 324, 487
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 3 cut(s) 32, 115, 166
BspANI GGCC 6 cut(s) 5, 44, 96, 160, 188, 278
BspCNI CTCAG 2 cut(s) 285, 364
BspPI GGATC 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 386
BssECI CCNNGG 4 cut(s) 6, 45, 75, 91
BssMI GATC 3 cut(s) 259, 324, 487
BssSI CACGAG 1 cut(s) 534
BssT1I CCWWGG 2 cut(s) 6, 75
Bst2BI CACGAG 1 cut(s) 534
Bst2UI CCWGG 3 cut(s) 92, 98, 347
Bst4CI ACNGT 2 cut(s) 251, 406
BstDEI CTNAG 4 cut(s) 162, 272, 351, 375
BstDSI CCRYGG 2 cut(s) 6, 45
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 3 cut(s) 16, 126, 132
BstKTI GATC 3 cut(s) 262, 327, 490
BstMAI GTCTC 2 cut(s) 64, 275
BstMBI GATC 3 cut(s) 259, 324, 487
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 3 cut(s) 92, 98, 347
BstSCI CCNGG 3 cut(s) 90, 96, 345
BstV1I GCAGC 5 cut(s) 27, 35, 38, 41, 466
BstV2I GAAGAC 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 393
BsuRI GGCC 6 cut(s) 5, 44, 96, 160, 188, 278
BtgI CCRYGG 2 cut(s) 6, 45
BtsCI GGATG 3 cut(s) 16, 126, 132
BtsIMutI CAGTG 1 cut(s) 469
Cfr13I GGNCC 3 cut(s) 94, 158, 276
CseI GACGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 389
CviAII CATG 1 cut(s) 7
CviQI GTAC 1 cut(s) 389
DdeI CTNAG 4 cut(s) 162, 272, 351, 375
DpnI GATC 3 cut(s) 261, 326, 489
DpnII GATC 3 cut(s) 259, 324, 487
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
EaeI YGGCCR 2 cut(s) 3, 42
Eco130I CCWWGG 2 cut(s) 6, 75
Eco57I CTGAAG 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 452
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 3 cut(s) 90, 96, 345
EcoT14I CCWWGG 2 cut(s) 6, 75
ErhI CCWWGG 2 cut(s) 6, 75
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 10
FaiI YATR 3 cut(s) 8, 410, 445
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 219, 251
FaqI GGGAC 1 cut(s) 410
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 5 cut(s) 16, 24, 27, 30, 480
FokI GGATG 3 cut(s) 23, 113, 119
Fsp4HI GCNGC 5 cut(s) 16, 24, 27, 30, 480
GluI GCNGC 5 cut(s) 16, 24, 27, 30, 480
GsaI CCCAGC 1 cut(s) 397
HaeIII GGCC 6 cut(s) 5, 44, 96, 160, 188, 278
HgaI GACGC 1 cut(s) 388
Hin1II CATG 1 cut(s) 10
HinfI GANTC 2 cut(s) 287, 455
HphI GGTGA 3 cut(s) 97, 331, 505
Hpy188I TCNGA 2 cut(s) 299, 354
Hpy188III TCNNGA 4 cut(s) 67, 140, 257, 452
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 118, 146
HpyCH4III ACNGT 2 cut(s) 251, 406
HpyCH4V TGCA 2 cut(s) 23, 529
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 4 cut(s) 162, 272, 351, 375
Hsp92II CATG 1 cut(s) 10
Kzo9I GATC 3 cut(s) 259, 324, 487
Lsp1109I GCAGC 5 cut(s) 27, 35, 38, 41, 466
LweI GCATC 1 cut(s) 355
MaeIII GTNAC 1 cut(s) 337
MalI GATC 3 cut(s) 261, 326, 489
MboI GATC 3 cut(s) 259, 324, 487
MboII GAAGA 3 cut(s) 239, 440, 508
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 464
MmeI TCCRAC 2 cut(s) 147, 492
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 359
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 243, 264
MslI CAYNNNNRTG 1 cut(s) 413
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 92, 98, 347
MvaI CCWGG 3 cut(s) 92, 98, 347
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 259, 324, 487
NlaIII CATG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 359
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 5 cut(s) 17, 25, 28, 31, 481
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
Psp6I CCWGG 3 cut(s) 90, 96, 345
PspFI CCCAGC 1 cut(s) 393
PspGI CCWGG 3 cut(s) 90, 96, 345
PspPI GGNCC 3 cut(s) 94, 158, 276
RsaI GTAC 1 cut(s) 390
RsaNI GTAC 1 cut(s) 389
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 2 cut(s) 243, 264
SatI GCNGC 5 cut(s) 16, 24, 27, 30, 480
Sau3AI GATC 3 cut(s) 259, 324, 487
Sau96I GGNCC 3 cut(s) 94, 158, 276
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 3 cut(s) 92, 98, 347
SetI ASST 8 cut(s) 77, 102, 110, 212, 226, 295, 429, 527
SfaNI GCATC 1 cut(s) 355
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 2 cut(s) 182, 506
SmoI CTYRAG 2 cut(s) 182, 506
Sse9I AATT 1 cut(s) 38
SsiI CCGC 3 cut(s) 32, 115, 166
StyD4I CCNGG 3 cut(s) 90, 96, 345
StyI CCWWGG 2 cut(s) 6, 75
TaaI ACNGT 2 cut(s) 251, 406
TaqI TCGA 2 cut(s) 111, 363
TasI AATT 1 cut(s) 38
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 2 cut(s) 243, 264
Tru9I TTAA 2 cut(s) 243, 264
TscAI CASTG 1 cut(s) 469
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 5 cut(s) 15, 23, 26, 29, 479
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 1 cut(s) 434
TspRI CASTG 1 cut(s) 469
XagI CCTNNNNNAGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.