RchiOBHm_Chr3g0467191

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001597
Physical Location & Seq
Forward (+)
13438523 .. 13439429
907 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43317

Sequence Viewer

Length: 555 bp
ATGAAGAAGAAGATGGCCAGGGATGGCAGCAATGCAGCAGCAGCGGAGGAATTGGCCGTCGGTTGCTTCGTCTCCATCAAGACCACCTTGGGCGACAACTTCCAGGGCCAGGTCATCACCTTCGACCGCCCCTCCAACATCCTCATCCTTCAAGAGGGTTTGAAAGGAGGGCCTAAGCGGAACATCAGGCTACTCAAGGCCAACTATATCAAGGAGTTGAGCTATTTGGGTCAAGCTGAAGACCCACTTGACAGTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCAGAGAGGAATCAGCTATCAGACAAGCAGAGGCAGAGTGTGAGAGGATCGGAGTTGGTGTCACCAGCCAGGCTCAGAACATTTTCGATGCCTTGTCTAAGACTCCATATCTTCCCGAGTCTGTCAGTGGAGGAACTCCTGCTGCCCATGATCGGGTGAAGAAAGTGCTTGAGTTGGAGAAGACTTTTCCCTCAAGGGATAAGTCATTGTGGGTATGTTTGTATACTCGGCCTTCTCTGAGGGGAGCATTATCTTTTTTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

184

Amino Acids

20.33

Weight (kDa)

8.87

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM12_LSM PF21166 16 - 72 9.4e-14 LSM12, LSM domain
AD PF09793 90 - 131 2.1e-09 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 512
AciI CCGC 3 cut(s) 44, 127, 178
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 2 cut(s) 15, 54
AcuI CTGAAG 1 cut(s) 258
AfiI CCNNNNNNNGG 4 cut(s) 109, 154, 441, 442
AgsI TTSAA 2 cut(s) 152, 163
AjnI CCWGG 4 cut(s) 17, 102, 108, 357
AluBI AGCT 3 cut(s) 222, 236, 305
AluI AGCT 3 cut(s) 222, 236, 305
Alw26I GTCTC 2 cut(s) 76, 287
AlwI GGATC 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 6 cut(s) 15, 54, 106, 170, 198, 518
ApeKI GCWGC 5 cut(s) 27, 35, 38, 41, 431
Asp700I GAANNNNTTC 1 cut(s) 371
AspS9I GGNCC 2 cut(s) 106, 170
AsuHPI GGTGA 3 cut(s) 109, 343, 457
AvaI CYCGRG 1 cut(s) 404
BalI TGGCCA 1 cut(s) 17
BbsI GAAGAC 2 cut(s) 246, 476
BbvI GCAGC 5 cut(s) 39, 47, 50, 53, 418
BccI CCATC 3 cut(s) 7, 17, 83
BceAI ACGGC 1 cut(s) 41
BciT130I CCWGG 4 cut(s) 19, 104, 110, 359
BcoDI GTCTC 2 cut(s) 76, 287
BisI GCNGC 5 cut(s) 28, 36, 39, 42, 432
BlsI GCNGC 5 cut(s) 29, 37, 40, 43, 433
Bme1390I CCNGG 4 cut(s) 19, 104, 110, 359
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 2 cut(s) 106, 170
BmrFI CCNGG 4 cut(s) 19, 104, 110, 359
BmsI GCATC 1 cut(s) 367
BpiI GAAGAC 2 cut(s) 246, 476
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 3 cut(s) 179, 466, 479
BsaJI CCNNGG 3 cut(s) 18, 87, 103
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 333, 363
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 154, 441, 442
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 4 cut(s) 19, 104, 110, 359
BseDI CCNNGG 3 cut(s) 18, 87, 103
BseGI GGATG 3 cut(s) 28, 138, 144
BseLI CCNNNNNNNGG 4 cut(s) 109, 154, 441, 442
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 3 cut(s) 298, 377, 518
BseXI GCAGC 5 cut(s) 39, 47, 50, 53, 418
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 6 cut(s) 17, 56, 108, 172, 200, 520
BsiEI CGRYCG 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 404
BslI CCNNNNNNNGG 4 cut(s) 109, 154, 441, 442
BsmAI GTCTC 2 cut(s) 76, 287
BsmBI CGTCTC 1 cut(s) 76
BsnI GGCC 6 cut(s) 17, 56, 108, 172, 200, 520
BsoBI CYCGRG 1 cut(s) 404
Bsp143I GATC 3 cut(s) 271, 336, 439
BspACI CCGC 3 cut(s) 44, 127, 178
BspANI GGCC 6 cut(s) 17, 56, 108, 172, 200, 520
BspCNI CTCAG 3 cut(s) 297, 376, 519
BspPI GGATC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 18, 87, 103
BssMI GATC 3 cut(s) 271, 336, 439
BssNAI GTATAC 1 cut(s) 513
BssT1I CCWWGG 1 cut(s) 87
Bst1107I GTATAC 1 cut(s) 513
Bst2UI CCWGG 4 cut(s) 19, 104, 110, 359
Bst4CI ACNGT 2 cut(s) 254, 263
BstDEI CTNAG 5 cut(s) 174, 284, 363, 387, 527
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 3 cut(s) 28, 138, 144
BstKTI GATC 3 cut(s) 274, 339, 442
BstMAI GTCTC 2 cut(s) 76, 287
BstMBI GATC 3 cut(s) 271, 336, 439
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstNI CCWGG 4 cut(s) 19, 104, 110, 359
BstSCI CCNGG 4 cut(s) 17, 102, 108, 357
BstV1I GCAGC 5 cut(s) 39, 47, 50, 53, 418
BstV2I GAAGAC 2 cut(s) 246, 476
BstZ17I GTATAC 1 cut(s) 513
BsuRI GGCC 6 cut(s) 17, 56, 108, 172, 200, 520
BtsCI GGATG 3 cut(s) 28, 138, 144
BtsIMutI CAGTG 1 cut(s) 421
Cfr13I GGNCC 2 cut(s) 106, 170
CviAII CATG 1 cut(s) 437
DdeI CTNAG 5 cut(s) 174, 284, 363, 387, 527
DpnI GATC 3 cut(s) 273, 338, 441
DpnII GATC 3 cut(s) 271, 336, 439
EaeI YGGCCR 2 cut(s) 15, 54
Eco130I CCWWGG 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 258
Eco88I CYCGRG 1 cut(s) 404
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 170
EcoRII CCWGG 4 cut(s) 17, 102, 108, 357
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 1 cut(s) 440
FaiI YATR 5 cut(s) 207, 397, 438, 505, 513
FalI AAGNNNNNCTT 4 cut(s) 71, 103, 231, 263
FatI CATG 1 cut(s) 436
FblI GTMKAC 1 cut(s) 512
Fnu4HI GCNGC 5 cut(s) 28, 36, 39, 42, 432
FokI GGATG 3 cut(s) 35, 125, 131
Fsp4HI GCNGC 5 cut(s) 28, 36, 39, 42, 432
GluI GCNGC 5 cut(s) 28, 36, 39, 42, 432
HaeIII GGCC 6 cut(s) 17, 56, 108, 172, 200, 520
Hin1II CATG 1 cut(s) 440
HinfI GANTC 3 cut(s) 299, 391, 407
HphI GGTGA 3 cut(s) 109, 343, 457
Hpy166II GTNNAC 1 cut(s) 513
Hpy188I TCNGA 5 cut(s) 287, 311, 341, 366, 528
Hpy188III TCNNGA 3 cut(s) 79, 152, 404
Hpy8I GTNNAC 1 cut(s) 513
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 130, 158, 531
HpyCH4III ACNGT 2 cut(s) 254, 263
HpyCH4V TGCA 1 cut(s) 35
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 5 cut(s) 174, 284, 363, 387, 527
Hsp92II CATG 1 cut(s) 440
Kzo9I GATC 3 cut(s) 271, 336, 439
LmnI GCTCC 1 cut(s) 533
Lsp1109I GCAGC 5 cut(s) 39, 47, 50, 53, 418
LweI GCATC 1 cut(s) 367
MaeIII GTNAC 2 cut(s) 263, 349
MalI GATC 3 cut(s) 273, 338, 441
MboI GATC 3 cut(s) 271, 336, 439
MboII GAAGA 7 cut(s) 16, 19, 22, 251, 392, 460, 481
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 1 cut(s) 50
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 2 cut(s) 385, 416
MmeI TCCRAC 2 cut(s) 159, 444
Mox20I TGGCCA 1 cut(s) 17
MroXI GAANNNNTTC 1 cut(s) 371
MscI TGGCCA 1 cut(s) 17
MseI TTAA 2 cut(s) 276, 553
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 44
MspR9I CCNGG 4 cut(s) 19, 104, 110, 359
MvaI CCWGG 4 cut(s) 19, 104, 110, 359
MwoI GCNNNNNNNGC 1 cut(s) 41
NdeII GATC 3 cut(s) 271, 336, 439
NlaIII CATG 1 cut(s) 440
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 5 cut(s) 29, 37, 40, 43, 433
PleI GAGTC 2 cut(s) 385, 415
PpsI GAGTC 2 cut(s) 385, 415
Psp6I CCWGG 4 cut(s) 17, 102, 108, 357
PspGI CCWGG 4 cut(s) 17, 102, 108, 357
PspPI GGNCC 2 cut(s) 106, 170
SaqAI TTAA 2 cut(s) 276, 553
SatI GCNGC 5 cut(s) 28, 36, 39, 42, 432
Sau3AI GATC 3 cut(s) 271, 336, 439
Sau96I GGNCC 2 cut(s) 106, 170
SchI GAGTC 2 cut(s) 385, 416
ScrFI CCNGG 4 cut(s) 19, 104, 110, 359
SetI ASST 7 cut(s) 89, 114, 122, 224, 238, 270, 307
SfaNI GCATC 1 cut(s) 367
SmlI CTYRAG 3 cut(s) 194, 458, 481
SmoI CTYRAG 3 cut(s) 194, 458, 481
Sse9I AATT 1 cut(s) 50
SsiI CCGC 3 cut(s) 44, 127, 178
StyD4I CCNGG 4 cut(s) 17, 102, 108, 357
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 254, 263
TaqI TCGA 2 cut(s) 123, 375
TasI AATT 1 cut(s) 50
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 2 cut(s) 276, 553
Tru9I TTAA 2 cut(s) 276, 553
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 5 cut(s) 27, 35, 38, 41, 431
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 421
XagI CCTNNNNNAGG 1 cut(s) 152
XmiI GTMKAC 1 cut(s) 512
XmnI GAANNNNTTC 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.