Rw1G006150

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001597
Physical Location & Seq
Forward (+)
12487471 .. 12488469
999 bp
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UTR
Exon/CDS
Intron
Rw1G006150.1

Sequence Viewer

Length: 429 bp
ATGGCCATGGATGGCAGCAATGCAGCAGCAGCGGAGGAATTGGCCGTGGGTTGCTTCGTCTCCATCAAGACCACCTTGGGCGACGACTTCCAGGGCCAGGGTTTGAAAGGAGGGCCTAAGTGGAATATCAGGCTACTCAAGGCCAACTATATCAAGGAGTTGAGCTATTTGGGTCAAGCTGAAGATCCACTTGACATTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCAGAGATACTCAGGTAGTTTACTTGCTACCCAATTTGGAACAAGCAGAGGCAGAGTGTGAGAGGATCGGAGTTGGTGTCACAAGCCAGGCTCAGAGCATTTTCGATGCCTTGTCTAAGACGCTTCCAGTACGCTGGGACAAGACTGTCATAGTTGTGATGAATGAGGTTCGTGTGAGCAATCCATATCTTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

142

Amino Acids

15.61

Weight (kDa)

4.83

Isoelectric Point (pI)

29.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AD PF09793 89 - 140 2.9e-18 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
AciI CCGC 1 cut(s) 32
AclWI GGATC 2 cut(s) 179, 305
AcoI YGGCCR 2 cut(s) 3, 42
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 3 cut(s) 90, 96, 318
AluBI AGCT 2 cut(s) 165, 179
AluI AGCT 2 cut(s) 165, 179
Alw26I GTCTC 2 cut(s) 64, 230
AlwI GGATC 2 cut(s) 179, 305
AoxI GGCC 5 cut(s) 3, 42, 94, 113, 141
ApeKI GCWGC 4 cut(s) 15, 23, 26, 29
AspS9I GGNCC 2 cut(s) 94, 113
BaeI ACNNNNGTAYC 2 cut(s) 231, 264
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 4 cut(s) 27, 35, 38, 41
BccI CCATC 2 cut(s) 5, 71
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 92, 98, 320
BcoDI GTCTC 2 cut(s) 64, 230
BfaI CTAG 1 cut(s) 427
BisI GCNGC 4 cut(s) 16, 24, 27, 30
BlsI GCNGC 4 cut(s) 17, 25, 28, 31
Bme1390I CCNGG 3 cut(s) 92, 98, 320
BmgT120I GGNCC 2 cut(s) 94, 113
BmrFI CCNGG 3 cut(s) 92, 98, 320
BmsI GCATC 1 cut(s) 328
BpuEI CTTGAG 1 cut(s) 122
BsaJI CCNNGG 5 cut(s) 6, 45, 75, 91, 97
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 3 cut(s) 92, 98, 320
BseDI CCNNGG 5 cut(s) 6, 45, 75, 91, 97
BseGI GGATG 1 cut(s) 16
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 3 cut(s) 241, 257, 338
BseNI ACTGG 1 cut(s) 359
BseXI GCAGC 4 cut(s) 27, 35, 38, 41
BseYI CCCAGC 1 cut(s) 366
BshFI GGCC 5 cut(s) 5, 44, 96, 115, 143
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 2 cut(s) 64, 230
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 5 cut(s) 5, 44, 96, 115, 143
Bsp143I GATC 3 cut(s) 184, 214, 297
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 1 cut(s) 32
BspANI GGCC 5 cut(s) 5, 44, 96, 115, 143
BspCNI CTCAG 3 cut(s) 240, 256, 337
BspPI GGATC 2 cut(s) 179, 305
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 359
BssECI CCNNGG 5 cut(s) 6, 45, 75, 91, 97
BssMI GATC 3 cut(s) 184, 214, 297
BssT1I CCWWGG 2 cut(s) 6, 75
Bst2UI CCWGG 3 cut(s) 92, 98, 320
Bst4CI ACNGT 2 cut(s) 206, 379
BstDEI CTNAG 5 cut(s) 117, 227, 243, 324, 348
BstDSI CCRYGG 2 cut(s) 6, 45
BstF5I GGATG 1 cut(s) 16
BstKTI GATC 3 cut(s) 187, 217, 300
BstMAI GTCTC 2 cut(s) 64, 230
BstMBI GATC 3 cut(s) 184, 214, 297
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 3 cut(s) 92, 98, 320
BstSCI CCNGG 3 cut(s) 90, 96, 318
BstV1I GCAGC 4 cut(s) 27, 35, 38, 41
BstX2I RGATCY 1 cut(s) 184
BstXI CCANNNNNNTGG 1 cut(s) 366
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 5 cut(s) 5, 44, 96, 115, 143
BtgI CCRYGG 2 cut(s) 6, 45
BtsCI GGATG 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 94, 113
CseI GACGC 1 cut(s) 361
Csp6I GTAC 1 cut(s) 362
CviAII CATG 1 cut(s) 7
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 5 cut(s) 117, 227, 243, 324, 348
DpnI GATC 3 cut(s) 186, 216, 299
DpnII GATC 3 cut(s) 184, 214, 297
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 2 cut(s) 3, 42
Eco130I CCWWGG 2 cut(s) 6, 75
Eco57I CTGAAG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 113
EcoRII CCWGG 3 cut(s) 90, 96, 318
EcoT14I CCWWGG 2 cut(s) 6, 75
ErhI CCWWGG 2 cut(s) 6, 75
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 10
FaiI YATR 4 cut(s) 8, 150, 383, 418
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 174, 206
FaqI GGGAC 1 cut(s) 383
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 4 cut(s) 16, 24, 27, 30
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 4 cut(s) 16, 24, 27, 30
FspBI CTAG 1 cut(s) 427
GluI GCNGC 4 cut(s) 16, 24, 27, 30
GsaI CCCAGC 1 cut(s) 370
HaeIII GGCC 5 cut(s) 5, 44, 96, 115, 143
HgaI GACGC 1 cut(s) 361
Hin1II CATG 1 cut(s) 10
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 230, 302, 327
Hpy188III TCNNGA 1 cut(s) 67
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 1 cut(s) 86
HpyCH4III ACNGT 2 cut(s) 206, 379
HpyCH4V TGCA 1 cut(s) 23
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 5 cut(s) 117, 227, 243, 324, 348
Hsp92II CATG 1 cut(s) 10
Kzo9I GATC 3 cut(s) 184, 214, 297
Lsp1109I GCAGC 4 cut(s) 27, 35, 38, 41
LweI GCATC 1 cut(s) 328
MaeI CTAG 1 cut(s) 427
MaeIII GTNAC 2 cut(s) 206, 310
MalI GATC 3 cut(s) 186, 216, 299
MboI GATC 3 cut(s) 184, 214, 297
MboII GAAGA 2 cut(s) 194, 413
MflI RGATCY 1 cut(s) 184
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 38, 265
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 5 cut(s) 28, 104, 274, 288, 391
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 198, 219
MslI CAYNNNNRTG 1 cut(s) 386
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 92, 98, 320
MvaI CCWGG 3 cut(s) 92, 98, 320
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 184, 214, 297
NlaIII CATG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 310
PkrI GCNGC 4 cut(s) 17, 25, 28, 31
Psp6I CCWGG 3 cut(s) 90, 96, 318
PspFI CCCAGC 1 cut(s) 366
PspGI CCWGG 3 cut(s) 90, 96, 318
PspPI GGNCC 2 cut(s) 94, 113
PsuI RGATCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 2 cut(s) 198, 219
SatI GCNGC 4 cut(s) 16, 24, 27, 30
Sau3AI GATC 3 cut(s) 184, 214, 297
Sau96I GGNCC 2 cut(s) 94, 113
ScrFI CCNGG 3 cut(s) 92, 98, 320
SetI ASST 6 cut(s) 77, 167, 181, 213, 249, 402
SfaNI GCATC 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 2 cut(s) 38, 265
SsiI CCGC 1 cut(s) 32
SspMI CTAG 1 cut(s) 427
StyD4I CCNGG 3 cut(s) 90, 96, 318
StyI CCWWGG 2 cut(s) 6, 75
TaaI ACNGT 2 cut(s) 206, 379
TaqI TCGA 1 cut(s) 336
TasI AATT 2 cut(s) 38, 265
Tru1I TTAA 2 cut(s) 198, 219
Tru9I TTAA 2 cut(s) 198, 219
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 4 cut(s) 15, 23, 26, 29
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 1 cut(s) 407
XspI CTAG 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.