FvH4_5g08060

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001530
Physical Location & Seq
Forward (+)
4695613 .. 4696035
423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08060.t1

Sequence Viewer

Length: 423 bp
ATGGAGAATCACAGAGGATTTGAGCGTGTGTTCAGCTACTTTGATGAAAACGGCGACGGGAAGATTTCGCCCTCGGAGCTGAGAAACCGGCTAGGGTTGATGGGAGGAGAAATGCTGCAAAACGAGGCGGAGGTTGCCGTTGGGTATTTGGACTCGGACGGAGATGGCTTGCTGGGTCTGGATGATCTTGTTGAGTTGATGGAAGGTGGAGAAGAGGAAGAGAAAATGAAGGGTTTGAGAGAGGCTTTTGAAGTGTATGATTTGGAGGGGTGTGGGTTTATAACACCCAAGAGCTTGAAGAGAATGCTTAACAAGTTGGGTGAGTCCAAGTCTATTGAGGAATGCAAGGCGATGATCAGTCGGTTTGATTTGAATGGGGATGGCCTCATCAGCTTTGAGGAGTTCATAGTAATGATGCAATGA

Protein Analysis

141

Amino Acids

15.72

Weight (kDa)

4.36

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 7 - 30 8.5e-06 EF hand domain
EF-hand_7 PF13499 8 - 67 2.4e-10 EF-hand domain pair
EF-hand_5 PF13202 9 - 29 1.9e-06 EF hand
AIF-1 PF21008 71 - 139 8e-08 Allograft inflammatory factor 1
EF-hand_7 PF13499 78 - 139 1.6e-13 EF-hand domain pair
EF-hand_8 PF13833 94 - 140 1.3e-09 EF-hand domain pair
EF-hand_1 PF00036 114 - 140 4.3e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AciI CCGC 1 cut(s) 128
AgsI TTSAA 3 cut(s) 251, 298, 373
AluBI AGCT 4 cut(s) 36, 79, 294, 393
AluI AGCT 4 cut(s) 36, 79, 294, 393
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 332
BbvI GCAGC 1 cut(s) 102
BccI CCATC 4 cut(s) 94, 158, 193, 374
BceAI ACGGC 2 cut(s) 67, 122
BclI TGATCA 1 cut(s) 354
BfaI CTAG 1 cut(s) 92
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
BmsI GCATC 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 72
Bse118I RCCGGY 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 2 cut(s) 187, 385
BseMII CTCAG 1 cut(s) 71
BseRI GAGGAG 2 cut(s) 120, 413
BseXI GCAGC 1 cut(s) 102
BseYI CCCAGC 1 cut(s) 172
BshFI GGCC 1 cut(s) 384
BsiSI CCGG 1 cut(s) 88
BsmI GAATGC 2 cut(s) 309, 347
BsnI GGCC 1 cut(s) 384
Bsp143I GATC 2 cut(s) 184, 354
BspACI CCGC 1 cut(s) 128
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 87
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 2 cut(s) 184, 354
Bst6I CTCTTC 3 cut(s) 207, 213, 293
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 2 cut(s) 187, 385
BstKTI GATC 2 cut(s) 187, 357
BstMBI GATC 2 cut(s) 184, 354
BstMWI GCNNNNNNNGC 3 cut(s) 76, 134, 390
BstV1I GCAGC 1 cut(s) 102
BsuRI GGCC 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 365
BtsCI GGATG 2 cut(s) 187, 385
Cac8I GCNNGC 1 cut(s) 170
Cfr10I RCCGGY 1 cut(s) 87
CviJI RGCY 8 cut(s) 36, 79, 91, 168, 245, 294, 384, 393
CviKI_1 RGCY 8 cut(s) 36, 79, 91, 168, 245, 294, 384, 393
DdeI CTNAG 1 cut(s) 80
DpnI GATC 2 cut(s) 186, 356
DpnII GATC 2 cut(s) 184, 354
Eam1104I CTCTTC 3 cut(s) 207, 213, 293
EarI CTCTTC 3 cut(s) 207, 213, 293
EciI GGCGGA 1 cut(s) 143
FaiI YATR 3 cut(s) 258, 281, 407
FbaI TGATCA 1 cut(s) 354
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 2 cut(s) 194, 392
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 1 cut(s) 92
GluI GCNGC 1 cut(s) 116
GsaI CCCAGC 1 cut(s) 176
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 88
HinfI GANTC 3 cut(s) 7, 152, 323
HpaII CCGG 1 cut(s) 88
HphI GGTGA 1 cut(s) 332
Hpy188I TCNGA 2 cut(s) 76, 157
Hpy188III TCNNGA 1 cut(s) 179
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 2 cut(s) 197, 223
HpyCH4V TGCA 3 cut(s) 118, 345, 418
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 134, 390
HpyF3I CTNAG 1 cut(s) 80
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 2 cut(s) 184, 354
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 3 cut(s) 101, 158, 164
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 1 cut(s) 405
MaeI CTAG 1 cut(s) 92
MalI GATC 2 cut(s) 186, 356
MboI GATC 2 cut(s) 184, 354
MboII GAAGA 4 cut(s) 73, 224, 230, 310
MlyI GAGTC 2 cut(s) 146, 332
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 410
MspI CCGG 1 cut(s) 88
Mva1269I GAATGC 2 cut(s) 309, 347
MwoI GCNNNNNNNGC 3 cut(s) 76, 134, 390
NdeII GATC 2 cut(s) 184, 354
PctI GAATGC 2 cut(s) 309, 347
PfeI GAWTC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 117
PleI GAGTC 2 cut(s) 146, 331
PpsI GAGTC 2 cut(s) 146, 331
PsiI TTATAA 1 cut(s) 281
PspFI CCCAGC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 410
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 2 cut(s) 184, 354
SchI GAGTC 2 cut(s) 146, 332
SetI ASST 6 cut(s) 38, 81, 135, 208, 296, 395
SfaNI GCATC 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 410
SsiI CCGC 1 cut(s) 128
SspMI CTAG 1 cut(s) 92
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseI GCWGC 1 cut(s) 115
TspDTI ATGAA 3 cut(s) 60, 242, 394
TspGWI ACGGA 1 cut(s) 174
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.