Rh2BG674000

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001530
Physical Location & Seq
Forward (+)
88569889 .. 88574029
4141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG674000.1

Sequence Viewer

Length: 528 bp
ATGGCAAGTGGGAGTGATATAGAAAATGGTCAAGTGGATGAAAGCGTTTCAGTTCATGTAGAACGTAGAGGTATGACTGATGAACAATGGAAAAGCACCTTCAGTACAGAGCTTTTGATGGCCAAATCACCTTTGCCTTCTTCATGTTGCATCTTCAAAGTTCCTCAGATATCACCTTTGGAGCTCCAACAATGTGTTGAGGCAATAGGAGGTGAGCTTTGGTCGTCGGAGGCTGAGGCGGCAGTGGAGTACTTGGACTCCGACAGAGACAGGCTGCTGGGTTTCGACGACTTCGTTAAGTTCGTTGAAGGAGGAGATGAGGAAGAGAAGGTGAATGATTTGAGGGAGGCGTCTAAGATGTACTACATGGATGGATGTGGGTGCATTACGCCCAAAAGTTTGAAGAAGATGCTGAGTAGACTTGGTGAATCTAGGAGTGTTGATGAGTGCAATGTCATGATTGCTAGGTTTGATCTCAATGGAGATGGTGTCCTCAATTTTGATGAGTTTAAGGTCATGATGTTTTGA

Protein Analysis

175

Amino Acids

19.59

Weight (kDa)

4.39

Isoelectric Point (pI)

53.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 124 - 174 1.2e-07 EF-hand domain pair
EF-hand_8 PF13833 127 - 175 2.3e-10 EF-hand domain pair
EF-hand_1 PF00036 149 - 174 2.2e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 418
AciI CCGC 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 85
AcyI GRCGYC 1 cut(s) 350
AfaI GTAC 3 cut(s) 106, 251, 362
AgsI TTSAA 3 cut(s) 157, 308, 403
AluBI AGCT 3 cut(s) 112, 184, 217
AluI AGCT 3 cut(s) 112, 184, 217
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 261
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 274
AsuHPI GGTGA 5 cut(s) 120, 165, 224, 343, 437
BalI TGGCCA 1 cut(s) 122
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 186
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 261
BccI CCATC 3 cut(s) 112, 365, 479
BcoDI GTCTC 1 cut(s) 261
BfaI CTAG 2 cut(s) 432, 465
BisI GCNGC 2 cut(s) 240, 275
BlsI GCNGC 2 cut(s) 241, 276
BmcAI AGTACT 1 cut(s) 251
BmsI GCATC 2 cut(s) 159, 399
Bpu10I CCTNAGC 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 350
BsaXI ACNNNNNCTCC 4 cut(s) 242, 272, 474, 504
Bse3DI GCAATG 1 cut(s) 457
BseGI GGATG 3 cut(s) 43, 376, 380
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 3 cut(s) 179, 225, 404
BseRI GAGGAG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 261
BseYI CCCAGC 1 cut(s) 277
BshFI GGCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 261
BsnI GGCC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 1 cut(s) 472
BspACI CCGC 1 cut(s) 239
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 3 cut(s) 178, 226, 405
BspHI TCATGA 2 cut(s) 456, 516
BsrDI GCAATG 1 cut(s) 457
BssMI GATC 1 cut(s) 472
BssNI GRCGYC 1 cut(s) 350
Bst6I CTCTTC 1 cut(s) 318
BstACI GRCGYC 1 cut(s) 350
BstDEI CTNAG 4 cut(s) 165, 234, 354, 413
BstF5I GGATG 3 cut(s) 43, 376, 380
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 1 cut(s) 472
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 261
BsuRI GGCC 1 cut(s) 122
BtsCI GGATG 3 cut(s) 43, 376, 380
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 249
CciI TCATGA 2 cut(s) 456, 516
CseI GACGC 1 cut(s) 339
Csp6I GTAC 3 cut(s) 105, 250, 361
CviAII CATG 5 cut(s) 56, 144, 367, 457, 517
CviJI RGCY 6 cut(s) 112, 122, 184, 217, 233, 274
CviKI_1 RGCY 6 cut(s) 112, 122, 184, 217, 233, 274
CviQI GTAC 3 cut(s) 105, 250, 361
DdeI CTNAG 4 cut(s) 165, 234, 354, 413
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 171
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 184
EcoRV GATATC 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 5 cut(s) 59, 147, 370, 460, 520
FaiI YATR 7 cut(s) 20, 57, 74, 145, 368, 458, 518
FatI CATG 5 cut(s) 55, 143, 366, 456, 516
FblI GTMKAC 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 240, 275
FokI GGATG 3 cut(s) 50, 383, 387
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 2 cut(s) 240, 275
FspBI CTAG 2 cut(s) 432, 465
GluI GCNGC 2 cut(s) 240, 275
GsaI CCCAGC 1 cut(s) 281
HaeIII GGCC 1 cut(s) 122
HgaI GACGC 1 cut(s) 339
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 5 cut(s) 59, 147, 370, 460, 520
HinfI GANTC 2 cut(s) 257, 428
HphI GGTGA 5 cut(s) 120, 165, 224, 343, 437
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 3 cut(s) 168, 229, 262
Hpy188III TCNNGA 2 cut(s) 457, 517
Hpy8I GTNNAC 1 cut(s) 419
Hpy99I CGWCG 2 cut(s) 229, 290
HpyAV CCTTC 4 cut(s) 109, 147, 302, 322
HpyCH4IV ACGT 1 cut(s) 64
HpyCH4V TGCA 3 cut(s) 150, 384, 450
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 4 cut(s) 165, 234, 354, 413
HpySE526I ACGT 1 cut(s) 64
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 5 cut(s) 59, 147, 370, 460, 520
Kzo9I GATC 1 cut(s) 472
LmnI GCTCC 2 cut(s) 181, 189
LpnPI CCDG 2 cut(s) 256, 263
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 2 cut(s) 159, 399
MaeI CTAG 2 cut(s) 432, 465
MaeII ACGT 1 cut(s) 64
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 5 cut(s) 132, 145, 335, 415, 418
MhlI GDGCHC 1 cut(s) 186
MlsI TGGCCA 1 cut(s) 122
MluCI AATT 1 cut(s) 496
MluNI TGGCCA 1 cut(s) 122
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 3 cut(s) 207, 211, 285
Mox20I TGGCCA 1 cut(s) 122
MscI TGGCCA 1 cut(s) 122
MseI TTAA 2 cut(s) 297, 510
Msp20I TGGCCA 1 cut(s) 122
MwoI GCNNNNNNNGC 1 cut(s) 239
NdeII GATC 1 cut(s) 472
NlaIII CATG 5 cut(s) 59, 147, 370, 460, 520
PagI TCATGA 2 cut(s) 456, 516
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 300
PfeI GAWTC 1 cut(s) 428
PkrI GCNGC 2 cut(s) 241, 276
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 186
PspFI CCCAGC 1 cut(s) 277
RsaI GTAC 3 cut(s) 106, 251, 362
RsaNI GTAC 3 cut(s) 105, 250, 361
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 297, 510
SatI GCNGC 2 cut(s) 240, 275
Sau3AI GATC 1 cut(s) 472
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 1 cut(s) 251
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 2 cut(s) 159, 399
Sse9I AATT 1 cut(s) 496
SsiI CCGC 1 cut(s) 239
SspMI CTAG 2 cut(s) 432, 465
SstI GAGCTC 1 cut(s) 186
TaiI ACGT 1 cut(s) 67
TaqI TCGA 1 cut(s) 285
TasI AATT 1 cut(s) 496
TatI WGTACW 3 cut(s) 104, 249, 360
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 2 cut(s) 297, 510
Tru9I TTAA 2 cut(s) 297, 510
TscAI CASTG 1 cut(s) 249
TseI GCWGC 1 cut(s) 274
TspDTI ATGAA 4 cut(s) 44, 54, 96, 132
TspRI CASTG 1 cut(s) 249
XmiI GTMKAC 1 cut(s) 418
XspI CTAG 2 cut(s) 432, 465
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.