Rh2BG673200

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001530
Physical Location & Seq
Forward (+)
88456649 .. 88457778
1130 bp
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UTR
Exon/CDS
Intron
Rh2BG673200.1

Sequence Viewer

Length: 423 bp
ATGGACAAACTAGCACAATACGAGCGTGTTTTCAATCAATTCGATGGCAATGGAGACGGGAAGATTTCACCTTTGGAGCTCCAACAATGTGTCGAGGCAATAGGAGGTGAGCTTTCGTCGTCGGAGTCTGTGGCTGCGGTGGAGTACTTGGACTCCGACAAAGACGGCCTGCTGGGTTTCGACGACTTCGTTAAGTTCGTTGAAGGAGGAGATGAGGAAGAGAAGGTGAATGATTTGAGGGAGGCGTTTAAGATGTACGACATGGATGGATGTGGGTGCATTACGCCCAAAAGTTTGAAGAGGATGCTGAGTAGACTTGGTGAATCCAGGAGTGTTGATGAGTGCAAGGTCATGATTGCTAGGTTTGATCTCAATGGAGATGGTGTCCTCAATTTTGATGAGTTTAAGGTCATGATGTTTTGA

Protein Analysis

140

Amino Acids

15.67

Weight (kDa)

4.31

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 7 - 35 2.4e-06 EF-hand domain
EF-hand_7 PF13499 8 - 67 4.6e-11 EF-hand domain pair
EF-hand_5 PF13202 10 - 31 8.7e-08 EF hand
EF-hand_8 PF13833 19 - 68 4.6e-06 EF-hand domain pair
AIF-1 PF21008 65 - 139 9.4e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 77 - 139 2.6e-13 EF-hand domain pair
EF-hand_6 PF13405 78 - 107 5.4e-06 EF-hand domain
EF-hand_8 PF13833 92 - 140 1.4e-10 EF-hand domain pair
EF-hand_1 PF00036 114 - 139 6.2e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 313
AciI CCGC 1 cut(s) 137
AfaI GTAC 2 cut(s) 146, 257
AgsI TTSAA 3 cut(s) 34, 203, 298
AjnI CCWGG 1 cut(s) 326
AluBI AGCT 2 cut(s) 79, 112
AluI AGCT 2 cut(s) 79, 112
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 48
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 1 cut(s) 134
AsuHPI GGTGA 4 cut(s) 60, 119, 238, 332
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 121
BccI CCATC 3 cut(s) 38, 260, 374
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 2 cut(s) 11, 360
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
BmcAI AGTACT 1 cut(s) 146
Bme1390I CCNGG 1 cut(s) 328
BmrFI CCNGG 1 cut(s) 328
BmsI GCATC 1 cut(s) 294
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 369, 399
Bse3DI GCAATG 1 cut(s) 55
BseBI CCWGG 1 cut(s) 328
BseGI GGATG 3 cut(s) 271, 275, 309
BseMI GCAATG 1 cut(s) 55
BseMII CTCAG 1 cut(s) 299
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 121
BseYI CCCAGC 1 cut(s) 172
BshFI GGCC 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 81
BsmAI GTCTC 1 cut(s) 48
BsmBI CGTCTC 1 cut(s) 48
BsnI GGCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 367
BspACI CCGC 1 cut(s) 137
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 1 cut(s) 300
BspHI TCATGA 2 cut(s) 351, 411
BsrDI GCAATG 1 cut(s) 55
BssMI GATC 1 cut(s) 367
Bst2UI CCWGG 1 cut(s) 328
Bst6I CTCTTC 2 cut(s) 213, 293
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 1 cut(s) 308
BstF5I GGATG 3 cut(s) 271, 275, 309
BstKTI GATC 1 cut(s) 370
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 1 cut(s) 367
BstNI CCWGG 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 326
BstV1I GCAGC 1 cut(s) 121
BsuRI GGCC 1 cut(s) 168
BtsCI GGATG 3 cut(s) 271, 275, 309
Cac8I GCNNGC 1 cut(s) 170
CciI TCATGA 2 cut(s) 351, 411
Csp6I GTAC 2 cut(s) 145, 256
CviAII CATG 3 cut(s) 262, 352, 412
CviJI RGCY 4 cut(s) 79, 112, 134, 168
CviKI_1 RGCY 4 cut(s) 79, 112, 134, 168
CviQI GTAC 2 cut(s) 145, 256
DdeI CTNAG 1 cut(s) 308
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 213, 293
EarI CTCTTC 2 cut(s) 213, 293
Ecl136II GAGCTC 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 326
EcoT38I GRGCYC 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 3 cut(s) 265, 355, 415
FaiI YATR 3 cut(s) 263, 353, 413
FatI CATG 3 cut(s) 261, 351, 411
FblI GTMKAC 1 cut(s) 313
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 3 cut(s) 278, 282, 316
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 2 cut(s) 11, 360
GluI GCNGC 1 cut(s) 135
GsaI CCCAGC 1 cut(s) 176
HaeIII GGCC 1 cut(s) 168
Hin1II CATG 3 cut(s) 265, 355, 415
HinfI GANTC 3 cut(s) 125, 152, 323
HphI GGTGA 4 cut(s) 60, 119, 238, 332
Hpy166II GTNNAC 1 cut(s) 314
Hpy188I TCNGA 2 cut(s) 124, 157
Hpy188III TCNNGA 2 cut(s) 352, 412
Hpy8I GTNNAC 1 cut(s) 314
Hpy99I CGWCG 3 cut(s) 121, 124, 185
HpyAV CCTTC 2 cut(s) 197, 217
HpyCH4V TGCA 2 cut(s) 279, 345
HpyF3I CTNAG 1 cut(s) 308
Hsp92II CATG 3 cut(s) 265, 355, 415
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 2 cut(s) 76, 84
LpnPI CCDG 4 cut(s) 158, 182, 313, 340
Lsp1109I GCAGC 1 cut(s) 121
LweI GCATC 1 cut(s) 294
MaeI CTAG 2 cut(s) 11, 360
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 3 cut(s) 73, 230, 310
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 2 cut(s) 38, 391
MlyI GAGTC 2 cut(s) 134, 146
MmeI TCCRAC 3 cut(s) 102, 106, 180
MnlI CCTC 8 cut(s) 88, 98, 200, 208, 231, 235, 294, 398
MseI TTAA 3 cut(s) 192, 249, 405
MspR9I CCNGG 1 cut(s) 328
MvaI CCWGG 1 cut(s) 328
NdeII GATC 1 cut(s) 367
NlaIII CATG 3 cut(s) 265, 355, 415
PagI TCATGA 2 cut(s) 351, 411
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 195
PfeI GAWTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 326
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 2 cut(s) 133, 146
PpsI GAGTC 2 cut(s) 133, 146
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 326
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 1 cut(s) 326
RsaI GTAC 2 cut(s) 146, 257
RsaNI GTAC 2 cut(s) 145, 256
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 192, 249, 405
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 1 cut(s) 367
ScaI AGTACT 1 cut(s) 146
SchI GAGTC 2 cut(s) 134, 146
ScrFI CCNGG 1 cut(s) 328
SduI GDGCHC 1 cut(s) 81
SetI ASST 8 cut(s) 73, 81, 109, 114, 228, 351, 365, 411
SfaNI GCATC 1 cut(s) 294
Sse9I AATT 2 cut(s) 38, 391
SsiI CCGC 1 cut(s) 137
SspMI CTAG 2 cut(s) 11, 360
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 1 cut(s) 326
TaqI TCGA 3 cut(s) 42, 93, 180
TasI AATT 2 cut(s) 38, 391
TatI WGTACW 1 cut(s) 144
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 3 cut(s) 192, 249, 405
Tru9I TTAA 3 cut(s) 192, 249, 405
TseI GCWGC 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 313
XspI CTAG 2 cut(s) 11, 360
ZrmI AGTACT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.