FvH4_6g53150

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001530
Physical Location & Seq
Forward (+)
39028268 .. 39028969
702 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53150.t1

Sequence Viewer

Length: 423 bp
ATGGACAAACTAGCACAATACGAGCGTGTTTTCAATCAATTCGATGGAAACGGAGACGGGAAGATATCACCTATGGAGCTCCAACAATGCGTTGAGGCAATAGGAGGCGAGCTTTCGTCATCGGAGGCTGAGGCGGCGGTGGAGTACTTAGATTCCGACAGAGACGGGCTGCTGGGTTTCGACGACTTCGTAAAGTTTGTCGAAGGAGGAGATGAGGAAGAGAAGGTGAATGATTTGAGGGAGGCGTTTAAGATGTACGACACGGATGGATGTGGGTGCATTACGCCCAAGAGTTTGAAGAGGATGCTGAGTAGACTTGGTGAATCGAGAAGTGTTGATGAGTGCAAGGTCATGATTGCTAAGTTTGATCTCAACGGGGATGGTGTCCTCAATTTTGATGAGTTTAAGGTCATGATGTTTTGA

Protein Analysis

141

Amino Acids

15.67

Weight (kDa)

4.28

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 7 - 35 2.2e-06 EF-hand domain
EF-hand_7 PF13499 8 - 67 5.4e-12 EF-hand domain pair
EF-hand_5 PF13202 10 - 31 2.9e-08 EF hand
EF-hand_8 PF13833 19 - 68 1.5e-06 EF-hand domain pair
EF-hand_7 PF13499 77 - 139 9.5e-15 EF-hand domain pair
EF-hand_6 PF13405 78 - 107 4.4e-07 EF-hand domain
EF-hand_8 PF13833 92 - 140 1.5e-10 EF-hand domain pair
EF-hand_1 PF00036 114 - 139 2.9e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 313
AciI CCGC 2 cut(s) 134, 137
AfaI GTAC 2 cut(s) 146, 257
AgsI TTSAA 2 cut(s) 34, 298
AluBI AGCT 2 cut(s) 79, 112
AluI AGCT 2 cut(s) 79, 112
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 48, 156
ApeKI GCWGC 1 cut(s) 169
AsuHPI GGTGA 3 cut(s) 60, 238, 332
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 156
BccI CCATC 3 cut(s) 38, 260, 374
BcoDI GTCTC 2 cut(s) 48, 156
BfaI CTAG 1 cut(s) 11
BisI GCNGC 2 cut(s) 135, 170
BlsI GCNGC 2 cut(s) 136, 171
BmcAI AGTACT 1 cut(s) 146
BmsI GCATC 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 129
BseGI GGATG 4 cut(s) 271, 275, 309, 385
BseMII CTCAG 2 cut(s) 120, 299
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 156
BseYI CCCAGC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 81
BsmAI GTCTC 2 cut(s) 48, 156
BsmBI CGTCTC 2 cut(s) 48, 156
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 367
BspACI CCGC 2 cut(s) 134, 137
BspCNI CTCAG 2 cut(s) 121, 300
BspHI TCATGA 2 cut(s) 351, 411
BssMI GATC 1 cut(s) 367
Bst6I CTCTTC 2 cut(s) 213, 293
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 4 cut(s) 129, 148, 308, 360
BstF5I GGATG 4 cut(s) 271, 275, 309, 385
BstKTI GATC 1 cut(s) 370
BstMAI GTCTC 2 cut(s) 48, 156
BstMBI GATC 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstV1I GCAGC 1 cut(s) 156
BtsCI GGATG 4 cut(s) 271, 275, 309, 385
Cac8I GCNNGC 1 cut(s) 110
CciI TCATGA 2 cut(s) 351, 411
Csp6I GTAC 2 cut(s) 145, 256
CviAII CATG 2 cut(s) 352, 412
CviJI RGCY 4 cut(s) 79, 112, 128, 169
CviKI_1 RGCY 4 cut(s) 79, 112, 128, 169
CviQI GTAC 2 cut(s) 145, 256
DdeI CTNAG 4 cut(s) 129, 148, 308, 360
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 213, 293
EarI CTCTTC 2 cut(s) 213, 293
Ecl136II GAGCTC 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 81
Eco32I GATATC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 79
EcoRV GATATC 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 81
Esp3I CGTCTC 2 cut(s) 48, 156
FaeI CATG 2 cut(s) 355, 415
FaiI YATR 3 cut(s) 74, 353, 413
FatI CATG 2 cut(s) 351, 411
FblI GTMKAC 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 135, 170
FokI GGATG 4 cut(s) 278, 282, 316, 392
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 2 cut(s) 135, 170
FspBI CTAG 1 cut(s) 11
GluI GCNGC 2 cut(s) 135, 170
GsaI CCCAGC 1 cut(s) 176
Hin1II CATG 2 cut(s) 355, 415
HinfI GANTC 2 cut(s) 152, 323
HphI GGTGA 3 cut(s) 60, 238, 332
Hpy166II GTNNAC 1 cut(s) 314
Hpy188I TCNGA 2 cut(s) 124, 157
Hpy188III TCNNGA 3 cut(s) 327, 352, 412
Hpy8I GTNNAC 1 cut(s) 314
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 197, 217
HpyCH4V TGCA 2 cut(s) 279, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 4 cut(s) 129, 148, 308, 360
Hsp92II CATG 2 cut(s) 355, 415
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 2 cut(s) 76, 84
LpnPI CCDG 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 156
LweI GCATC 1 cut(s) 294
MaeI CTAG 1 cut(s) 11
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 3 cut(s) 73, 230, 310
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 2 cut(s) 38, 391
MmeI TCCRAC 2 cut(s) 106, 180
MseI TTAA 2 cut(s) 249, 405
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeII GATC 1 cut(s) 367
NlaIII CATG 2 cut(s) 355, 415
PagI TCATGA 2 cut(s) 351, 411
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 2 cut(s) 152, 323
PkrI GCNGC 2 cut(s) 136, 171
Psp124BI GAGCTC 1 cut(s) 81
PspFI CCCAGC 1 cut(s) 172
RsaI GTAC 2 cut(s) 146, 257
RsaNI GTAC 2 cut(s) 145, 256
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 249, 405
SatI GCNGC 2 cut(s) 135, 170
Sau3AI GATC 1 cut(s) 367
ScaI AGTACT 1 cut(s) 146
SduI GDGCHC 1 cut(s) 81
SetI ASST 6 cut(s) 73, 81, 114, 228, 351, 411
SfaNI GCATC 1 cut(s) 294
Sse9I AATT 2 cut(s) 38, 391
SsiI CCGC 2 cut(s) 134, 137
SspMI CTAG 1 cut(s) 11
SstI GAGCTC 1 cut(s) 81
TaqI TCGA 4 cut(s) 42, 180, 201, 326
TasI AATT 2 cut(s) 38, 391
TatI WGTACW 1 cut(s) 144
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 2 cut(s) 152, 323
Tru1I TTAA 2 cut(s) 249, 405
Tru9I TTAA 2 cut(s) 249, 405
TseI GCWGC 1 cut(s) 169
TspGWI ACGGA 2 cut(s) 66, 278
XmiI GTMKAC 1 cut(s) 313
XspI CTAG 1 cut(s) 11
ZrmI AGTACT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.