Prupe.3G303600_v2.0.a1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001530
Physical Location & Seq
Forward (+)
26689506 .. 26690311
806 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G303600.1

Sequence Viewer

Length: 432 bp
ATGGACAAAGTGGCACAATATGAGCGCGTTTTCAATCACTTTGATGCAAATGGAGATGGCAAAATATCACCTTTGGAGCTACAACAATGTGTCCGGGCAATCGGCGGAGAGCTGTCGTTGACAGAGGCAGAGGTGGCAGTGGAGCACCTGGACTCAGATGGAGATGGGCTGCTGTGTTTGGATGACTTTGTCAAGTTTGTTGACGGCGGAAGAGAAGAAGAGAAGGTGAATGATTTGAAGGAGGCGTTTAACATGTACGTGATGGATGATGGGTGTGGGTTTAGTTGCATTACACCCAAGAGCCTTAAGAGGATGCTGAGTAGACTTGGAGAGTCCAAGAGCGTTGATGACTGCAAGGTCATGATTTCCAGATTTGATCTCAATGGTGATGGGGTCCTCAATTTTGATGAGTTTAAGGTCATGATGTTGTGA

Protein Analysis

144

Amino Acids

15.96

Weight (kDa)

4.44

Isoelectric Point (pI)

22.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 356
AccI GTMKAC 1 cut(s) 322
AccII CGCG 1 cut(s) 27
AciI CCGC 2 cut(s) 105, 207
AfaI GTAC 1 cut(s) 257
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 2 cut(s) 34, 238
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 2 cut(s) 79, 112
AluI AGCT 2 cut(s) 79, 112
Alw21I GWGCWC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 169
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 394
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 3 cut(s) 60, 238, 398
AvaII GGWCC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 156
BccI CCATC 6 cut(s) 50, 152, 158, 256, 263, 383
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 149
BcnI CCSGG 1 cut(s) 95
BfrI CTTAAG 1 cut(s) 305
BisI GCNGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 171
Bme1390I CCNGG 2 cut(s) 95, 149
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 1 cut(s) 395
BmrFI CCNGG 2 cut(s) 95, 149
BmsI GCATC 2 cut(s) 34, 303
BpuMI CCSGG 1 cut(s) 95
BsaAI YACGTR 1 cut(s) 259
BseBI CCWGG 1 cut(s) 149
BseGI GGATG 3 cut(s) 187, 271, 318
BseMII CTCAG 2 cut(s) 168, 308
BseXI GCAGC 1 cut(s) 156
Bsh1236I CGCG 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 1 cut(s) 376
BspACI CCGC 2 cut(s) 105, 207
BspCNI CTCAG 2 cut(s) 167, 309
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 2 cut(s) 360, 420
BspLI GGNNCC 1 cut(s) 395
BspTI CTTAAG 1 cut(s) 305
BssMI GATC 1 cut(s) 376
Bst2UI CCWGG 1 cut(s) 149
Bst6I CTCTTC 2 cut(s) 205, 213
BstAFI CTTAAG 1 cut(s) 305
BstBAI YACGTR 1 cut(s) 259
BstDEI CTNAG 2 cut(s) 154, 317
BstF5I GGATG 3 cut(s) 187, 271, 318
BstFNI CGCG 1 cut(s) 27
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 1 cut(s) 379
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 2 cut(s) 93, 147
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 156
BtsCI GGATG 3 cut(s) 187, 271, 318
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
CciI TCATGA 2 cut(s) 360, 420
CfoI GCGC 1 cut(s) 27
Cfr13I GGNCC 1 cut(s) 394
Csp6I GTAC 1 cut(s) 256
CviAII CATG 3 cut(s) 253, 361, 421
CviJI RGCY 4 cut(s) 79, 112, 169, 303
CviKI_1 RGCY 4 cut(s) 79, 112, 169, 303
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 2 cut(s) 154, 317
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
Eam1104I CTCTTC 2 cut(s) 205, 213
EarI CTCTTC 2 cut(s) 205, 213
EciI GGCGGA 2 cut(s) 120, 222
Eco47I GGWCC 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 147
FaeI CATG 3 cut(s) 256, 364, 424
FaiI YATR 4 cut(s) 21, 254, 362, 422
FatI CATG 3 cut(s) 252, 360, 420
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 170
FokI GGATG 3 cut(s) 194, 278, 325
Fsp4HI GCNGC 1 cut(s) 170
GlaI GCGC 1 cut(s) 26
GluI GCNGC 1 cut(s) 170
HapII CCGG 1 cut(s) 94
HhaI GCGC 1 cut(s) 27
Hin1II CATG 3 cut(s) 256, 364, 424
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HincII GTYRAC 2 cut(s) 120, 202
HindII GTYRAC 2 cut(s) 120, 202
HinfI GANTC 2 cut(s) 152, 332
HpaII CCGG 1 cut(s) 94
HphI GGTGA 3 cut(s) 60, 238, 398
Hpy166II GTNNAC 3 cut(s) 120, 202, 323
Hpy188I TCNGA 1 cut(s) 157
Hpy188III TCNNGA 3 cut(s) 361, 369, 421
Hpy8I GTNNAC 3 cut(s) 120, 202, 323
HpyAV CCTTC 2 cut(s) 217, 232
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 3 cut(s) 47, 288, 354
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 2 cut(s) 154, 317
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 3 cut(s) 256, 364, 424
HspAI GCGC 1 cut(s) 25
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 2 cut(s) 76, 142
LpnPI CCDG 4 cut(s) 107, 134, 161, 382
Lsp1109I GCAGC 1 cut(s) 156
LweI GCATC 2 cut(s) 34, 303
MaeII ACGT 1 cut(s) 258
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 3 cut(s) 222, 227, 230
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 1 cut(s) 400
MlyI GAGTC 2 cut(s) 146, 341
MnlI CCTC 5 cut(s) 118, 124, 235, 303, 407
MseI TTAA 3 cut(s) 249, 306, 414
MslI CAYNNNNRTG 2 cut(s) 42, 257
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 1 cut(s) 94
MspR9I CCNGG 2 cut(s) 95, 149
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 1 cut(s) 95
NdeII GATC 1 cut(s) 376
NlaIII CATG 3 cut(s) 256, 364, 424
NlaIV GGNNCC 1 cut(s) 395
NspI RCATGY 1 cut(s) 256
PagI TCATGA 2 cut(s) 360, 420
PciI ACATGT 1 cut(s) 252
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 1 cut(s) 171
PleI GAGTC 2 cut(s) 146, 340
PpsI GAGTC 2 cut(s) 146, 340
Ppu21I YACGTR 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 394
PscI ACATGT 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 395
PspPI GGNCC 1 cut(s) 394
PspPPI RGGWCCY 1 cut(s) 394
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 2 cut(s) 42, 257
SaqAI TTAA 3 cut(s) 249, 306, 414
SatI GCNGC 1 cut(s) 170
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 394
SchI GAGTC 2 cut(s) 146, 341
ScrFI CCNGG 2 cut(s) 95, 149
SduI GDGCHC 1 cut(s) 147
SetI ASST 9 cut(s) 73, 81, 114, 135, 150, 228, 261, 360, 420
SfaNI GCATC 2 cut(s) 34, 303
SinI GGWCC 1 cut(s) 394
SmiMI CAYNNNNRTG 2 cut(s) 42, 257
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 1 cut(s) 400
SsiI CCGC 2 cut(s) 105, 207
StyD4I CCNGG 2 cut(s) 93, 147
TaiI ACGT 1 cut(s) 261
TasI AATT 1 cut(s) 400
Tru1I TTAA 3 cut(s) 249, 306, 414
Tru9I TTAA 3 cut(s) 249, 306, 414
TscAI CASTG 1 cut(s) 144
TseI GCWGC 1 cut(s) 169
TspRI CASTG 1 cut(s) 144
Tth111I GACNNNGTC 1 cut(s) 188
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 394
XceI RCATGY 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.